Click here to switch to the map view.

The map label for this gene is pinE [H]

Identifier: 120611070

GI number: 120611070

Start: 2620820

End: 2621413

Strand: Reverse

Name: pinE [H]

Synonym: Aave_2399

Alternate gene names: 120611070

Gene position: 2621413-2620820 (Counterclockwise)

Preceding gene: 120611071

Following gene: 120611063

Centisome position: 48.97

GC content: 62.63

Gene sequence:

>594_bases
ATGAGTCGGTTAGTCGGGTATGCGCGCGTCAGCACTAGGGAGCAGGAGACTGCGATGCAGCTTGCGGCGTTGCGGGCTGC
TGGGGTGGGCGTGGTGTATGAAGAGCAGGCCAGTGCAGTGCGCCAGCGGCCGGTATGGGAGCAGTGTTTAGCTGCCCTCA
AAAGAGGCGATGTGTTGGTGGTCTATAAGCTCGATCGGCTGGCGCGGTCCACGTCTCATTTCGTCACCACGTTTGACTTG
TTGCGAGCTCGTGGCGTTGGGTTCCGGAGCCTGACCGAAGCGATTGAAACTGTGACGCCTCAGGGGCGCATGTACCTACA
ACTATTGGCAGTGTTCGCGGAGTTTGAGCGGGAGTTGATCAGGGAGCGCTGCGTAGCTGGGCAGCGTGCCGCGCGGGCGG
CTGGGAAGACCTGGGGGCGTAAGGCCCAGTTAACGCGGGAAGAGGTGGCTCTCGCCGTTCAGGCGTGGCGCAGTGGGTGG
TACACCCAAGAAGTATTGGCGGCCATGCTGGGCGTGCCTGTCGGCACGTTGCGGTACCACGTCCATCGCCATGAGGGCCG
CGGGCGGCATGGGGCTTGGAGCACAGATAAGTGA

Upstream 100 bases:

>100_bases
TAGCGGCCCTTTTGGGCCGTTTTTCTTGCCGTCGCTACCGTGCCATTGCCGTCAAAACTTGGAGTTACGTTCCCGGAACG
AAAACACTATGGAGGGTTGA

Downstream 100 bases:

>100_bases
AGTTGTCCCGCTTCCACGGACGGTTATCGGCTTGATTCTCAAGCCGGGATTTGGTCTCTCTCGTTGATCTCCGTTGCCTG
ACACGCCTCGGCGACGACGC

Product: resolvase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 197; Mature: 196

Protein sequence:

>197_residues
MSRLVGYARVSTREQETAMQLAALRAAGVGVVYEEQASAVRQRPVWEQCLAALKRGDVLVVYKLDRLARSTSHFVTTFDL
LRARGVGFRSLTEAIETVTPQGRMYLQLLAVFAEFERELIRERCVAGQRAARAAGKTWGRKAQLTREEVALAVQAWRSGW
YTQEVLAAMLGVPVGTLRYHVHRHEGRGRHGAWSTDK

Sequences:

>Translated_197_residues
MSRLVGYARVSTREQETAMQLAALRAAGVGVVYEEQASAVRQRPVWEQCLAALKRGDVLVVYKLDRLARSTSHFVTTFDL
LRARGVGFRSLTEAIETVTPQGRMYLQLLAVFAEFERELIRERCVAGQRAARAAGKTWGRKAQLTREEVALAVQAWRSGW
YTQEVLAAMLGVPVGTLRYHVHRHEGRGRHGAWSTDK
>Mature_196_residues
SRLVGYARVSTREQETAMQLAALRAAGVGVVYEEQASAVRQRPVWEQCLAALKRGDVLVVYKLDRLARSTSHFVTTFDLL
RARGVGFRSLTEAIETVTPQGRMYLQLLAVFAEFERELIRERCVAGQRAARAAGKTWGRKAQLTREEVALAVQAWRSGWY
TQEVLAAMLGVPVGTLRYHVHRHEGRGRHGAWSTDK

Specific function: This protein catalyzes the inversion of an 1800-bp E.coli DNA fragment, the P region, which can exist in either orientation. The function of the inversion is not yet clear [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=175, Percent_Identity=45.1428571428571, Blast_Score=139, Evalue=1e-34,
Organism=Escherichia coli, GI1787638, Length=167, Percent_Identity=35.9281437125749, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1787827, Length=167, Percent_Identity=35.3293413173653, Blast_Score=91, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 22139; Mature: 22008

Theoretical pI: Translated: 10.80; Mature: 10.80

Prosite motif: PS00397 RECOMBINASES_1 ; PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLVGYARVSTREQETAMQLAALRAAGVGVVYEEQASAVRQRPVWEQCLAALKRGDVLV
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHCCHHHHHHHHHHCCCEEE
VYKLDRLARSTSHFVTTFDLLRARGVGFRSLTEAIETVTPQGRMYLQLLAVFAEFERELI
EEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
RERCVAGQRAARAAGKTWGRKAQLTREEVALAVQAWRSGWYTQEVLAAMLGVPVGTLRYH
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHH
VHRHEGRGRHGAWSTDK
HHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SRLVGYARVSTREQETAMQLAALRAAGVGVVYEEQASAVRQRPVWEQCLAALKRGDVLV
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHCCHHHHHHHHHHCCCEEE
VYKLDRLARSTSHFVTTFDLLRARGVGFRSLTEAIETVTPQGRMYLQLLAVFAEFERELI
EEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
RERCVAGQRAARAAGKTWGRKAQLTREEVALAVQAWRSGWYTQEVLAAMLGVPVGTLRYH
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHH
VHRHEGRGRHGAWSTDK
HHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3894006; 6310572; 8905232; 9278503 [H]