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The map label for this gene is tilS [H]

Identifier: 120610995

GI number: 120610995

Start: 2551771

End: 2552814

Strand: Direct

Name: tilS [H]

Synonym: Aave_2323

Alternate gene names: 120610995

Gene position: 2551771-2552814 (Clockwise)

Preceding gene: 120610994

Following gene: 120610996

Centisome position: 47.67

GC content: 73.37

Gene sequence:

>1044_bases
ATGACGCTGGCTTTCGATACGGCCATGGACCGTTTCCGGCCGGCGCTGCCCCTGGGCGTGGCGCTGAGCGGCGGGGCGGA
TTCGACGGCGCTGCTGCACGCCTGCGCGCAGCGCTGGCCGGGGCAGGTGCATGCGCTGCACGTGCACCATGGATTGCAGG
CCGCGGCGGATGGTTTCGAAACTCATTGCCGGGCGCTCTGCGGGCGGCTGGGTGTGCCGCTGCGCATCGCGCGTGTGGAT
GCGCGCCATGCTGGGGGTGAGAGCCCCGAGGATGCGGCCCGGCAGCGGCGCTATGAAGCCCTGCGGGACCTGGCCGGCGG
CCAGCAGGGAGCCCATCAGGCAGGTACGGACGGGGTCATGCCGGAGATGGAAGCGGGGCAGGGCGGACCGGGAGGCGATG
GGGACCCGGATAGGCCCGCGGCCGGGCGACCCGTGCTGGCCTCCGTTGCGCTGGCCCAGCATGCCGACGACCAGGTGGAA
ACCGTGTTGCTCGCGCTCTCGCGCGGCGCCGGGGTGGCGGGGCTCGCCGCCATGCCGGCCTTCTGGCGGCGGGGCGGCAT
CGACTGGCACCGGCCGCTGCTGGACGTCGCAGGCGCGGACGTACGCGCCTGGCTGACCGCGCGCGGCGAGGGCTGGGTCG
AGGATCCGACGAACGCGGACGAGCGCTATACCCGAAACCGCATCCGCGCGGGGCTGCTGCCGGCGCTGCAGGCCGCGTTT
CCCCAGTTCCGCGACACCTTCGCGCGCAGTGCCTCGCACGCGGCGGAAGCTGCCGTGCTGCTGCGCGAACTGGCCGGGGC
CGATCTGCAGGCCACGGGCCTTCCTCCGCGCATCGCCGTGCTGCGCGCGCTGGGCCGGGAACGCCAGGCCAACCTCCTGC
GCCACTGGCTGCGCACGGCGCATGCCACCACGCCCACGGCTGCGCAGTTGGGCGAACTGCTCGACCAGATCGCTGCCTGC
GCCACGCGCGGCCACCGCATCCGCATCAAAGTGGGACGGGGCTTCGTGGTGCGCCAGGGCGACGAGCTCGGTTGGTACAA
TTGA

Upstream 100 bases:

>100_bases
CTGCGCCACGAATGATTGATATGTTCAGGGCATTCGTATCCATGCCTGCTGCACGGCCCTTCGGGGCCGTTTTCGTTTTC
CCTGGGCGAGGGGCGGCGCG

Downstream 100 bases:

>100_bases
CGGGTTTTGGTCTTCTCTCTCATTCGCACGAAACACGGCGGCGCGCCAAAACACCTCAGCCACAGAGCATAGGGTGCCTA
CGAGGTCCCGGGGCCTGTGA

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MTLAFDTAMDRFRPALPLGVALSGGADSTALLHACAQRWPGQVHALHVHHGLQAAADGFETHCRALCGRLGVPLRIARVD
ARHAGGESPEDAARQRRYEALRDLAGGQQGAHQAGTDGVMPEMEAGQGGPGGDGDPDRPAAGRPVLASVALAQHADDQVE
TVLLALSRGAGVAGLAAMPAFWRRGGIDWHRPLLDVAGADVRAWLTARGEGWVEDPTNADERYTRNRIRAGLLPALQAAF
PQFRDTFARSASHAAEAAVLLRELAGADLQATGLPPRIAVLRALGRERQANLLRHWLRTAHATTPTAAQLGELLDQIAAC
ATRGHRIRIKVGRGFVVRQGDELGWYN

Sequences:

>Translated_347_residues
MTLAFDTAMDRFRPALPLGVALSGGADSTALLHACAQRWPGQVHALHVHHGLQAAADGFETHCRALCGRLGVPLRIARVD
ARHAGGESPEDAARQRRYEALRDLAGGQQGAHQAGTDGVMPEMEAGQGGPGGDGDPDRPAAGRPVLASVALAQHADDQVE
TVLLALSRGAGVAGLAAMPAFWRRGGIDWHRPLLDVAGADVRAWLTARGEGWVEDPTNADERYTRNRIRAGLLPALQAAF
PQFRDTFARSASHAAEAAVLLRELAGADLQATGLPPRIAVLRALGRERQANLLRHWLRTAHATTPTAAQLGELLDQIAAC
ATRGHRIRIKVGRGFVVRQGDELGWYN
>Mature_346_residues
TLAFDTAMDRFRPALPLGVALSGGADSTALLHACAQRWPGQVHALHVHHGLQAAADGFETHCRALCGRLGVPLRIARVDA
RHAGGESPEDAARQRRYEALRDLAGGQQGAHQAGTDGVMPEMEAGQGGPGGDGDPDRPAAGRPVLASVALAQHADDQVET
VLLALSRGAGVAGLAAMPAFWRRGGIDWHRPLLDVAGADVRAWLTARGEGWVEDPTNADERYTRNRIRAGLLPALQAAFP
QFRDTFARSASHAAEAAVLLRELAGADLQATGLPPRIAVLRALGRERQANLLRHWLRTAHATTPTAAQLGELLDQIAACA
TRGHRIRIKVGRGFVVRQGDELGWYN

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=327, Percent_Identity=35.474006116208, Blast_Score=131, Evalue=7e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946 [H]

EC number: NA

Molecular weight: Translated: 36968; Mature: 36837

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLAFDTAMDRFRPALPLGVALSGGADSTALLHACAQRWPGQVHALHVHHGLQAAADGFE
CEECHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHH
THCRALCGRLGVPLRIARVDARHAGGESPEDAARQRRYEALRDLAGGQQGAHQAGTDGVM
HHHHHHHHHCCCCEEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PEMEAGQGGPGGDGDPDRPAAGRPVLASVALAQHADDQVETVLLALSRGAGVAGLAAMPA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHH
FWRRGGIDWHRPLLDVAGADVRAWLTARGEGWVEDPTNADERYTRNRIRAGLLPALQAAF
HHHHCCCCHHHHHHHHCCCCCEEHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
PQFRDTFARSASHAAEAAVLLRELAGADLQATGLPPRIAVLRALGRERQANLLRHWLRTA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHH
HATTPTAAQLGELLDQIAACATRGHRIRIKVGRGFVVRQGDELGWYN
CCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEECCCCCCCCC
>Mature Secondary Structure 
TLAFDTAMDRFRPALPLGVALSGGADSTALLHACAQRWPGQVHALHVHHGLQAAADGFE
EECHHHHHHHHCCCCCCCEEECCCCCHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHH
THCRALCGRLGVPLRIARVDARHAGGESPEDAARQRRYEALRDLAGGQQGAHQAGTDGVM
HHHHHHHHHCCCCEEEEEEHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PEMEAGQGGPGGDGDPDRPAAGRPVLASVALAQHADDQVETVLLALSRGAGVAGLAAMPA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHH
FWRRGGIDWHRPLLDVAGADVRAWLTARGEGWVEDPTNADERYTRNRIRAGLLPALQAAF
HHHHCCCCHHHHHHHHCCCCCEEHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
PQFRDTFARSASHAAEAAVLLRELAGADLQATGLPPRIAVLRALGRERQANLLRHWLRTA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHH
HATTPTAAQLGELLDQIAACATRGHRIRIKVGRGFVVRQGDELGWYN
CCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA