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The map label for this gene is acnB [H]

Identifier: 120610884

GI number: 120610884

Start: 2429415

End: 2432006

Strand: Direct

Name: acnB [H]

Synonym: Aave_2210

Alternate gene names: 120610884

Gene position: 2429415-2432006 (Clockwise)

Preceding gene: 120610883

Following gene: 120610885

Centisome position: 45.39

GC content: 67.09

Gene sequence:

>2592_bases
ATGTTGAAAGCCTACCGTGACCATGTGGCCGAGCGCGCCGCCCTGGGCATCCCGCCCCTGCCGCTCGACGCGAAGCAGGT
CGCCGAACTGATCGAGCTGATCAAGAACCCGCCCGCCGGCGAGGAAGCGTTCCTGCTGGACCTGCTCACGCACCGCGTGC
CCCCGGGCGTGGACGATGCCGCCAAGGTCAAGGCCAGCTTCCTGGCCGCCGTGGCGCACGGCGACGTGAAGGTGGGCCTG
ATCTCCAAGGCCAAGGCCACGGAGCTGCTGGGCACCATGGTGGGCGGCTACAACGTCCATCCCCTGATCGAGCTGCTGGA
CGACGCCGAAGTGGCGGGCGTGGCCGCCGAGGGCCTGAAGAAGACGCTGCTGATGTTCGACTTCTTCAACGACGTGGCCG
AGAAGGCCAAGGCCGGCAACGCCCGTGCCAAGGAAGTGATGCAGTCGTGGGCCGACGCCGAATGGTTCACGAGCCGCCCC
GAAGTCGAGAAGAAGATCACCGTCACCGTCTTCAAGGTGCCCGGCGAGACCAACACCGATGACCTCTCCCCGGCGCCCGA
CGCCTGGAGCCGCCCGGACATCCCGCTGCACTACCTCGCCATGCTGAAGAACACCCGTCCCGACGCGGCCTTCAAGCCCG
AGGAAGACGGCAAGCGCGGCCCGATGCAGTTCATCGAGGACCTGAAGAAGAAGGGCCATGTCGTGGCCTACGTGGGCGAC
GTGGTGGGCACGGGCTCGTCCCGCAAGTCGGCCACCAACAGCGTGATCTGGGCCACCGGCCAGGACATTCCCTTCGTGCC
GAACAAGCGCTTCGGCGGCGTGACGCTGGGCGGCAAGATCGCTCCCATCTTCTTCAACACGCAGGAAGACTCGGGCTCGC
TGCCGATCGAGGTCGATGTGTCGAAGCTGGAAATGGGCGACGTGATCGACGTGCTGCCCTATGAAGGCAAGCTGGTGCGC
AACGGCGAAACCGTCGCCGAGTTCCAGCTCAAGAGCGACGTGCTGCTCGACGAAGTGCGCGCCGGCGGCCGCATCAACCT
GATCATCGGCCGCTCGCTGACCGCCAAGGCGCGCGAGTTCCTGGGCCTGCCCGCATCCACCGCATTCCGCCTGCCGCAGG
CGCCCGCCGAATCCAAGGCCGGCTTCACGCTGGCGCAGAAGATGGTCGGCCGCGCCGTGGGCCTGCCGGAAGGCCAGGGC
GTGCGCCCCGGTACCTACTGCGAGCCGAAGATGACCACCGTGGGCAGCCAGGACACCACCGGCCCGATGACCCGCGACGA
GCTGAAGGACCTGGCCTGCCTGGGCTTCTCGGCCGACATGGTGATGCAGTCGTTCTGCCACACGGCCGCCTATCCGAAGC
CCGTGGACGTGAAGACGCACCGCGAGCTGCCCGCCTTCATCAGCAACCGCGGCGGCGTCGCGCTGCGCCCCGGCGACGGC
GTGATCCACAGCTGGCTGAACCGCCTGCTGCTGCCCGACACCGTGGGCACCGGCGGTGATTCGCACACCCGCTTCCCGAT
CGGCATCTCGTTCCCGGCGGGCTCCGGCCTCGTGGCCTTCGGCGCCGCCACCGGCGTGATGCCGCTGGACATGCCCGAAT
CCGTGCTGGTGCGCTTCAAGGGCGAACTGCAGCCCGGCGTGACGCTGCGCGACCTGGTGCATGCCATTCCGCTGTACGCC
ATCAAGCAGGGCCTGCTGACGGTGGCCAAGGCCGGCAAGAAGAACATCTTCTCGGGCCGCATCCTGGAAATCGAGGGCCT
GCCGGACCTGAAGGTGGAACAGGCGTTCGAGCTGTCCGACGCCTCCGCCGAGCGCTCGGCCGCCGGCTGCACGATCAAGC
TCAACCCCGAGCCGATCAAGGAATACCTCACCAGCAACGTCGTGCTGATGAAGAACATGATCGCCGACGGCTACGCCGAC
GCGCGCACCCTCAAGCGCCGCATCGAGAAGGTCGAGGCCTGGCTGGCCAACCCCGAGCTGCTGGAGGCCGACAAGGACGC
CGAGTACGCCGCCGTGATCGAGATCGACCTGGCCGACATCACGGAGCCGATCGTCTGCTGCCCGAACGATCCGGACGACG
CCAAGTTCCTCTCGGAAGTGGCCGGCACCCAGATCGACGAAGCCTTCATCGGTTCGTGCATGACCAACATCGGCCACTTC
CGCGCAGCCGCCAAGCTGCTGGGCGGCCAGCGCGACATTCCCGTCAAGCTGTGGGTCGCGCCGCCGACCAAGATGGACCA
GAACGAGCTGATCAAGGAAGGCCACTACGCCGCGTTCGGCACGGCCGGCGCCCGCACCGAAATGCCGGGCTGCTCGCTGT
GCATGGGCAACCAGGCGCAGGTGCGCGAGGGCGCGACGGTGATCTCCACCTCCACCCGCAACTTCCCGAACCGCCTGGGC
AAGAACACCAACGTGTTCCTGGGCTCGGCCGAACTGGCCGCCATCGCCTCGCGCATCGGCAAGCTGCCCACCAAGGAGGA
ATACCTCAAGGAAATGGGCGTGATCGATGCCGACAAGGCGAGCGTCTATCGCTACATGAACTTCGACCAGATCGAGGAAT
ACGCGGACGCCGCCAAGTCCGTGGCGGCCTGA

Upstream 100 bases:

>100_bases
CTGCGGCGTCCTGGCCTGCGATGGACGGGCGCCGCCCGCGGCGCCCGCAGGACTGGCTTTGCCCCCAAAAATCCAACGAT
CGAACCCACCGGAGAACACC

Downstream 100 bases:

>100_bases
AGGCCGGGCGGGCCCCGGGGGCCCGCCCGCTTCCGCTGCCGGACTGACGTGCCGCACTGCAAGGGCCCCGGGGAGCGATC
CCCGGGGCCTTTGCTTTGTC

Product: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

Products: NA

Alternate protein names: Aconitase 2; 2-methylisocitrate dehydratase; Citrate hydro-lyase 2 [H]

Number of amino acids: Translated: 863; Mature: 863

Protein sequence:

>863_residues
MLKAYRDHVAERAALGIPPLPLDAKQVAELIELIKNPPAGEEAFLLDLLTHRVPPGVDDAAKVKASFLAAVAHGDVKVGL
ISKAKATELLGTMVGGYNVHPLIELLDDAEVAGVAAEGLKKTLLMFDFFNDVAEKAKAGNARAKEVMQSWADAEWFTSRP
EVEKKITVTVFKVPGETNTDDLSPAPDAWSRPDIPLHYLAMLKNTRPDAAFKPEEDGKRGPMQFIEDLKKKGHVVAYVGD
VVGTGSSRKSATNSVIWATGQDIPFVPNKRFGGVTLGGKIAPIFFNTQEDSGSLPIEVDVSKLEMGDVIDVLPYEGKLVR
NGETVAEFQLKSDVLLDEVRAGGRINLIIGRSLTAKAREFLGLPASTAFRLPQAPAESKAGFTLAQKMVGRAVGLPEGQG
VRPGTYCEPKMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYPKPVDVKTHRELPAFISNRGGVALRPGDG
VIHSWLNRLLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFGAATGVMPLDMPESVLVRFKGELQPGVTLRDLVHAIPLYA
IKQGLLTVAKAGKKNIFSGRILEIEGLPDLKVEQAFELSDASAERSAAGCTIKLNPEPIKEYLTSNVVLMKNMIADGYAD
ARTLKRRIEKVEAWLANPELLEADKDAEYAAVIEIDLADITEPIVCCPNDPDDAKFLSEVAGTQIDEAFIGSCMTNIGHF
RAAAKLLGGQRDIPVKLWVAPPTKMDQNELIKEGHYAAFGTAGARTEMPGCSLCMGNQAQVREGATVISTSTRNFPNRLG
KNTNVFLGSAELAAIASRIGKLPTKEEYLKEMGVIDADKASVYRYMNFDQIEEYADAAKSVAA

Sequences:

>Translated_863_residues
MLKAYRDHVAERAALGIPPLPLDAKQVAELIELIKNPPAGEEAFLLDLLTHRVPPGVDDAAKVKASFLAAVAHGDVKVGL
ISKAKATELLGTMVGGYNVHPLIELLDDAEVAGVAAEGLKKTLLMFDFFNDVAEKAKAGNARAKEVMQSWADAEWFTSRP
EVEKKITVTVFKVPGETNTDDLSPAPDAWSRPDIPLHYLAMLKNTRPDAAFKPEEDGKRGPMQFIEDLKKKGHVVAYVGD
VVGTGSSRKSATNSVIWATGQDIPFVPNKRFGGVTLGGKIAPIFFNTQEDSGSLPIEVDVSKLEMGDVIDVLPYEGKLVR
NGETVAEFQLKSDVLLDEVRAGGRINLIIGRSLTAKAREFLGLPASTAFRLPQAPAESKAGFTLAQKMVGRAVGLPEGQG
VRPGTYCEPKMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYPKPVDVKTHRELPAFISNRGGVALRPGDG
VIHSWLNRLLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFGAATGVMPLDMPESVLVRFKGELQPGVTLRDLVHAIPLYA
IKQGLLTVAKAGKKNIFSGRILEIEGLPDLKVEQAFELSDASAERSAAGCTIKLNPEPIKEYLTSNVVLMKNMIADGYAD
ARTLKRRIEKVEAWLANPELLEADKDAEYAAVIEIDLADITEPIVCCPNDPDDAKFLSEVAGTQIDEAFIGSCMTNIGHF
RAAAKLLGGQRDIPVKLWVAPPTKMDQNELIKEGHYAAFGTAGARTEMPGCSLCMGNQAQVREGATVISTSTRNFPNRLG
KNTNVFLGSAELAAIASRIGKLPTKEEYLKEMGVIDADKASVYRYMNFDQIEEYADAAKSVAA
>Mature_863_residues
MLKAYRDHVAERAALGIPPLPLDAKQVAELIELIKNPPAGEEAFLLDLLTHRVPPGVDDAAKVKASFLAAVAHGDVKVGL
ISKAKATELLGTMVGGYNVHPLIELLDDAEVAGVAAEGLKKTLLMFDFFNDVAEKAKAGNARAKEVMQSWADAEWFTSRP
EVEKKITVTVFKVPGETNTDDLSPAPDAWSRPDIPLHYLAMLKNTRPDAAFKPEEDGKRGPMQFIEDLKKKGHVVAYVGD
VVGTGSSRKSATNSVIWATGQDIPFVPNKRFGGVTLGGKIAPIFFNTQEDSGSLPIEVDVSKLEMGDVIDVLPYEGKLVR
NGETVAEFQLKSDVLLDEVRAGGRINLIIGRSLTAKAREFLGLPASTAFRLPQAPAESKAGFTLAQKMVGRAVGLPEGQG
VRPGTYCEPKMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYPKPVDVKTHRELPAFISNRGGVALRPGDG
VIHSWLNRLLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFGAATGVMPLDMPESVLVRFKGELQPGVTLRDLVHAIPLYA
IKQGLLTVAKAGKKNIFSGRILEIEGLPDLKVEQAFELSDASAERSAAGCTIKLNPEPIKEYLTSNVVLMKNMIADGYAD
ARTLKRRIEKVEAWLANPELLEADKDAEYAAVIEIDLADITEPIVCCPNDPDDAKFLSEVAGTQIDEAFIGSCMTNIGHF
RAAAKLLGGQRDIPVKLWVAPPTKMDQNELIKEGHYAAFGTAGARTEMPGCSLCMGNQAQVREGATVISTSTRNFPNRLG
KNTNVFLGSAELAAIASRIGKLPTKEEYLKEMGVIDADKASVYRYMNFDQIEEYADAAKSVAA

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]

COG id: COG1049

COG function: function code C; Aconitase B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Escherichia coli, GI2367097, Length=865, Percent_Identity=74.1040462427746, Blast_Score=1264, Evalue=0.0,
Organism=Escherichia coli, GI1786259, Length=377, Percent_Identity=25.9946949602122, Blast_Score=70, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6322261, Length=400, Percent_Identity=28.25, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR004406
- InterPro:   IPR015930
- InterPro:   IPR015933
- InterPro:   IPR015929 [H]

Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]

EC number: =4.2.1.3; =4.2.1.99 [H]

Molecular weight: Translated: 92845; Mature: 92845

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKAYRDHVAERAALGIPPLPLDAKQVAELIELIKNPPAGEEAFLLDLLTHRVPPGVDDA
CCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHH
AKVKASFLAAVAHGDVKVGLISKAKATELLGTMVGGYNVHPLIELLDDAEVAGVAAEGLK
HHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHH
KTLLMFDFFNDVAEKAKAGNARAKEVMQSWADAEWFTSRPEVEKKITVTVFKVPGETNTD
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHCCCCCCCCCEEEEEEEECCCCCCCC
DLSPAPDAWSRPDIPLHYLAMLKNTRPDAAFKPEEDGKRGPMQFIEDLKKKGHVVAYVGD
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECC
VVGTGSSRKSATNSVIWATGQDIPFVPNKRFGGVTLGGKIAPIFFNTQEDSGSLPIEVDV
HHCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEECCEEEEEEEECCCCCCCCCEEEEH
SKLEMGDVIDVLPYEGKLVRNGETVAEFQLKSDVLLDEVRAGGRINLIIGRSLTAKAREF
HHHCCCCEEEECCCCCEEEECCCCEEEEHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHH
LGLPASTAFRLPQAPAESKAGFTLAQKMVGRAVGLPEGQGVRPGTYCEPKMTTVGSQDTT
HCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCC
GPMTRDELKDLACLGFSADMVMQSFCHTAAYPKPVDVKTHRELPAFISNRGGVALRPGDG
CCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCEEECCCCC
VIHSWLNRLLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFGAATGVMPLDMPESVLVRFK
HHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCEEEECCCCCCCCCCCCHHHHHEEC
GELQPGVTLRDLVHAIPLYAIKQGLLTVAKAGKKNIFSGRILEIEGLPDLKVEQAFELSD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHCCCC
ASAERSAAGCTIKLNPEPIKEYLTSNVVLMKNMIADGYADARTLKRRIEKVEAWLANPEL
CCCHHHHCCCEEEECHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHH
LEADKDAEYAAVIEIDLADITEPIVCCPNDPDDAKFLSEVAGTQIDEAFIGSCMTNIGHF
CCCCCCCCEEEEEEEEHHHCCCCEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
RAAAKLLGGQRDIPVKLWVAPPTKMDQNELIKEGHYAAFGTAGARTEMPGCSLCMGNQAQ
HHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHCCCCEEEECCCCCCCCCCCCEECCCHHH
VREGATVISTSTRNFPNRLGKNTNVFLGSAELAAIASRIGKLPTKEEYLKEMGVIDADKA
HHCCCEEEECCCCHHHHHHCCCCEEEECCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCHH
SVYRYMNFDQIEEYADAAKSVAA
HHHHHCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLKAYRDHVAERAALGIPPLPLDAKQVAELIELIKNPPAGEEAFLLDLLTHRVPPGVDDA
CCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHH
AKVKASFLAAVAHGDVKVGLISKAKATELLGTMVGGYNVHPLIELLDDAEVAGVAAEGLK
HHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHH
KTLLMFDFFNDVAEKAKAGNARAKEVMQSWADAEWFTSRPEVEKKITVTVFKVPGETNTD
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHCCCCCCCCCEEEEEEEECCCCCCCC
DLSPAPDAWSRPDIPLHYLAMLKNTRPDAAFKPEEDGKRGPMQFIEDLKKKGHVVAYVGD
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECC
VVGTGSSRKSATNSVIWATGQDIPFVPNKRFGGVTLGGKIAPIFFNTQEDSGSLPIEVDV
HHCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEECCEEEEEEEECCCCCCCCCEEEEH
SKLEMGDVIDVLPYEGKLVRNGETVAEFQLKSDVLLDEVRAGGRINLIIGRSLTAKAREF
HHHCCCCEEEECCCCCEEEECCCCEEEEHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHH
LGLPASTAFRLPQAPAESKAGFTLAQKMVGRAVGLPEGQGVRPGTYCEPKMTTVGSQDTT
HCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCC
GPMTRDELKDLACLGFSADMVMQSFCHTAAYPKPVDVKTHRELPAFISNRGGVALRPGDG
CCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHCCCCCEEECCCCC
VIHSWLNRLLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFGAATGVMPLDMPESVLVRFK
HHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCEEEECCCCCCCCCCCCHHHHHEEC
GELQPGVTLRDLVHAIPLYAIKQGLLTVAKAGKKNIFSGRILEIEGLPDLKVEQAFELSD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHCCCC
ASAERSAAGCTIKLNPEPIKEYLTSNVVLMKNMIADGYADARTLKRRIEKVEAWLANPEL
CCCHHHHCCCEEEECHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHH
LEADKDAEYAAVIEIDLADITEPIVCCPNDPDDAKFLSEVAGTQIDEAFIGSCMTNIGHF
CCCCCCCCEEEEEEEEHHHCCCCEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
RAAAKLLGGQRDIPVKLWVAPPTKMDQNELIKEGHYAAFGTAGARTEMPGCSLCMGNQAQ
HHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHCCCCEEEECCCCCCCCCCCCEECCCHHH
VREGATVISTSTRNFPNRLGKNTNVFLGSAELAAIASRIGKLPTKEEYLKEMGVIDADKA
HHCCCEEEECCCCHHHHHHCCCCEEEECCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCHH
SVYRYMNFDQIEEYADAAKSVAA
HHHHHCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]