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The map label for this gene is folA [H]

Identifier: 120610135

GI number: 120610135

Start: 1595885

End: 1596382

Strand: Reverse

Name: folA [H]

Synonym: Aave_1448

Alternate gene names: 120610135

Gene position: 1596382-1595885 (Counterclockwise)

Preceding gene: 120610136

Following gene: 120610134

Centisome position: 29.82

GC content: 69.48

Gene sequence:

>498_bases
ATGACACTGCGACTCATCTACGCACGGGCCGCCAATGGCGGCATCGGTCTCCACAACGCCATGCCCTGGCACCTGCCCGA
AGACCTGGCGCATTTCAAGCAGCTCACGCTGGGCGCCACGGTGGTCATGGGGCGCAAGACCTGGGATTCCCTGCCTGCCC
GCTTCCGTCCCCTGCCCGGCCGCACGAACATCGTGGTCACGCGCCAGGCCGGCTGGCACGCGGACGGCGCCCTGTCCGCC
TCCAGCCTGGAGCAGGCGGTGGAACTGGCCCGGCCGCATGGCGATACGGTATGGGTGATGGGCGGTGCGCAGATCTACGC
CCAGGCCCTTCCGATGGCGGACGGCGTGGAGGTCACCGAAATCCACCGGGATTTCGAAGCGGACGCCTTCGCCCCCGTGC
TGGGCCCCGAATGGGAGGAGACCGCGCGCACCCCCGTGCAGACCTCCGCCGGCAGTGCGGCCCTTCCCTTTTCCTTCGTC
AGCTACCGGCGCCGCTGA

Upstream 100 bases:

>100_bases
TGCTCACGCGCCGCAAATGGCTGCAGTGGGGGATGTATGGCGTCGCAGGCCTGGGAATCGCCATCGGCGTCCTCGCGGCC
CTCCACCTCTGAACAATGCC

Downstream 100 bases:

>100_bases
AGCCGCCCGCCGGCAAGCTCCGTCCTTCCTCGCATCACTCTCCCTCCTATGAAGCTGGCCACCTGGAACGTCAACTCCCT
CTCCATACGGCTGCCCCAGG

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 165; Mature: 164

Protein sequence:

>165_residues
MTLRLIYARAANGGIGLHNAMPWHLPEDLAHFKQLTLGATVVMGRKTWDSLPARFRPLPGRTNIVVTRQAGWHADGALSA
SSLEQAVELARPHGDTVWVMGGAQIYAQALPMADGVEVTEIHRDFEADAFAPVLGPEWEETARTPVQTSAGSAALPFSFV
SYRRR

Sequences:

>Translated_165_residues
MTLRLIYARAANGGIGLHNAMPWHLPEDLAHFKQLTLGATVVMGRKTWDSLPARFRPLPGRTNIVVTRQAGWHADGALSA
SSLEQAVELARPHGDTVWVMGGAQIYAQALPMADGVEVTEIHRDFEADAFAPVLGPEWEETARTPVQTSAGSAALPFSFV
SYRRR
>Mature_164_residues
TLRLIYARAANGGIGLHNAMPWHLPEDLAHFKQLTLGATVVMGRKTWDSLPARFRPLPGRTNIVVTRQAGWHADGALSAS
SLEQAVELARPHGDTVWVMGGAQIYAQALPMADGVEVTEIHRDFEADAFAPVLGPEWEETARTPVQTSAGSAALPFSFVS
YRRR

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=152, Percent_Identity=34.2105263157895, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI28827793, Length=146, Percent_Identity=34.2465753424658, Blast_Score=78, Evalue=3e-15,
Organism=Escherichia coli, GI1786233, Length=165, Percent_Identity=36.3636363636364, Blast_Score=97, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17505899, Length=181, Percent_Identity=34.2541436464088, Blast_Score=99, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6324810, Length=125, Percent_Identity=32, Blast_Score=81, Evalue=7e-17,
Organism=Drosophila melanogaster, GI116008026, Length=155, Percent_Identity=31.6129032258064, Blast_Score=86, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24647458, Length=155, Percent_Identity=31.6129032258064, Blast_Score=86, Evalue=8e-18,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18001; Mature: 17869

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLRLIYARAANGGIGLHNAMPWHLPEDLAHFKQLTLGATVVMGRKTWDSLPARFRPLPG
CEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCEEEEECCCCHHCCCHHCCCCCC
RTNIVVTRQAGWHADGALSASSLEQAVELARPHGDTVWVMGGAQIYAQALPMADGVEVTE
CCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECHHHHHHHCCCCCCCCHHH
IHRDFEADAFAPVLGPEWEETARTPVQTSAGSAALPFSFVSYRRR
HHHCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHCCC
>Mature Secondary Structure 
TLRLIYARAANGGIGLHNAMPWHLPEDLAHFKQLTLGATVVMGRKTWDSLPARFRPLPG
EEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCEEEEECCCCHHCCCHHCCCCCC
RTNIVVTRQAGWHADGALSASSLEQAVELARPHGDTVWVMGGAQIYAQALPMADGVEVTE
CCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECHHHHHHHCCCCCCCCHHH
IHRDFEADAFAPVLGPEWEETARTPVQTSAGSAALPFSFVSYRRR
HHHCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6434541 [H]