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The map label for this gene is xthA [H]

Identifier: 120610134

GI number: 120610134

Start: 1595060

End: 1595836

Strand: Reverse

Name: xthA [H]

Synonym: Aave_1447

Alternate gene names: 120610134

Gene position: 1595836-1595060 (Counterclockwise)

Preceding gene: 120610135

Following gene: 120610100

Centisome position: 29.81

GC content: 64.48

Gene sequence:

>777_bases
ATGAAGCTGGCCACCTGGAACGTCAACTCCCTCTCCATACGGCTGCCCCAGGTGCTGGCCTGGCTGGCCGACAACCCCGT
GGATGCCATCGGCCTGCAGGAACTCAAGCTGGTGGACGAGAAATTCCCCCACGATGCCTTCGAATCCGCCGGCTACCACG
CCGTGAGCTTCGGGCAGAAAACCTACAACGGGGTCGCCATCCTGAGCCGCACGCCCGTACGCGACGTGGTGCGCAACATC
CCCGGGCACGAAGACACCCAGGCCCGCGTGATCGCGGCGACGCTGGACACGCCCCAAGGCCCGCTCCGGTTCATCAACTG
CTACTTCGTGAACGGCCAGGCCCCGGGCACGGAAAAGTTCGCCTACAAGATGCTCTGGCTGGCCGCGCTGCACCGATGGG
TCAGGGAGGAACTGCTCGCCCATCCTCGGCTGGTGCTCGTGGGCGACTTCAACGTAGCGCCGGAAGACCGTGACTCCTTC
GATCCCGTGGGCTTGAAAGACACCATCCACCACACCGTGGAAGAGCGCACCCATTTCCAGAGCCTTCTGCAACTGGGCCT
CACGGACGCCTACCGCATGTTCGAGCAACCCGAGAAAAGCTTCTCGTGGTGGGACTACCGCATGCTGGGTTTCCAGAAGA
ACCGGGGCTTGCGCATCGACCATATCCTGGTGAGCGAAGCCCTGCGCGGCCAAGTCACCGCGTGCACGATCGACCGGCAA
CCTCGCAAGAACCCGCAGCCCAGCGACCACGCGCCGGTCGTGGCCACGCTCGGCTGA

Upstream 100 bases:

>100_bases
CGCCGGCAGTGCGGCCCTTCCCTTTTCCTTCGTCAGCTACCGGCGCCGCTGAAGCCGCCCGCCGGCAAGCTCCGTCCTTC
CTCGCATCACTCTCCCTCCT

Downstream 100 bases:

>100_bases
CACTGCCAGCCGACAACGCGCAAAACAAAAAAGCCGCGGCCCGCAGCGCAACGCGGCTGCGGGCTTCGCGGCTTATCACC
TGCCAGCGGCGGGCAGGCAA

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MKLATWNVNSLSIRLPQVLAWLADNPVDAIGLQELKLVDEKFPHDAFESAGYHAVSFGQKTYNGVAILSRTPVRDVVRNI
PGHEDTQARVIAATLDTPQGPLRFINCYFVNGQAPGTEKFAYKMLWLAALHRWVREELLAHPRLVLVGDFNVAPEDRDSF
DPVGLKDTIHHTVEERTHFQSLLQLGLTDAYRMFEQPEKSFSWWDYRMLGFQKNRGLRIDHILVSEALRGQVTACTIDRQ
PRKNPQPSDHAPVVATLG

Sequences:

>Translated_258_residues
MKLATWNVNSLSIRLPQVLAWLADNPVDAIGLQELKLVDEKFPHDAFESAGYHAVSFGQKTYNGVAILSRTPVRDVVRNI
PGHEDTQARVIAATLDTPQGPLRFINCYFVNGQAPGTEKFAYKMLWLAALHRWVREELLAHPRLVLVGDFNVAPEDRDSF
DPVGLKDTIHHTVEERTHFQSLLQLGLTDAYRMFEQPEKSFSWWDYRMLGFQKNRGLRIDHILVSEALRGQVTACTIDRQ
PRKNPQPSDHAPVVATLG
>Mature_258_residues
MKLATWNVNSLSIRLPQVLAWLADNPVDAIGLQELKLVDEKFPHDAFESAGYHAVSFGQKTYNGVAILSRTPVRDVVRNI
PGHEDTQARVIAATLDTPQGPLRFINCYFVNGQAPGTEKFAYKMLWLAALHRWVREELLAHPRLVLVGDFNVAPEDRDSF
DPVGLKDTIHHTVEERTHFQSLLQLGLTDAYRMFEQPEKSFSWWDYRMLGFQKNRGLRIDHILVSEALRGQVTACTIDRQ
PRKNPQPSDHAPVVATLG

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=265, Percent_Identity=30.188679245283, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI18375503, Length=265, Percent_Identity=30.188679245283, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI18375501, Length=265, Percent_Identity=30.188679245283, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1788046, Length=270, Percent_Identity=34.8148148148148, Blast_Score=140, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI71989536, Length=271, Percent_Identity=28.0442804428044, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI221330655, Length=267, Percent_Identity=27.7153558052434, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI17136678, Length=267, Percent_Identity=27.7153558052434, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29247; Mature: 29247

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS00728 AP_NUCLEASE_F1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLATWNVNSLSIRLPQVLAWLADNPVDAIGLQELKLVDEKFPHDAFESAGYHAVSFGQK
CEEEEECCCCEEEEHHHHHHHHHCCCCCHHCHHHHHHHHHHCCCHHHHHCCCEEEECCCC
TYNGVAILSRTPVRDVVRNIPGHEDTQARVIAATLDTPQGPLRFINCYFVNGQAPGTEKF
CCCCEEEEECCCHHHHHHHCCCCCCCCEEEEEEECCCCCCCEEEEEEEEECCCCCCHHHH
AYKMLWLAALHRWVREELLAHPRLVLVGDFNVAPEDRDSFDPVGLKDTIHHTVEERTHFQ
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
SLLQLGLTDAYRMFEQPEKSFSWWDYRMLGFQKNRGLRIDHILVSEALRGQVTACTIDRQ
HHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCEEHHHHHHHHHCCCEEEEEECCC
PRKNPQPSDHAPVVATLG
CCCCCCCCCCCCEEEECC
>Mature Secondary Structure
MKLATWNVNSLSIRLPQVLAWLADNPVDAIGLQELKLVDEKFPHDAFESAGYHAVSFGQK
CEEEEECCCCEEEEHHHHHHHHHCCCCCHHCHHHHHHHHHHCCCHHHHHCCCEEEECCCC
TYNGVAILSRTPVRDVVRNIPGHEDTQARVIAATLDTPQGPLRFINCYFVNGQAPGTEKF
CCCCEEEEECCCHHHHHHHCCCCCCCCEEEEEEECCCCCCCEEEEEEEEECCCCCCHHHH
AYKMLWLAALHRWVREELLAHPRLVLVGDFNVAPEDRDSFDPVGLKDTIHHTVEERTHFQ
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
SLLQLGLTDAYRMFEQPEKSFSWWDYRMLGFQKNRGLRIDHILVSEALRGQVTACTIDRQ
HHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCEEHHHHHHHHHCCCEEEEEECCC
PRKNPQPSDHAPVVATLG
CCCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]