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The map label for this gene is leuD

Identifier: 120609909

GI number: 120609909

Start: 1346875

End: 1347528

Strand: Reverse

Name: leuD

Synonym: Aave_1222

Alternate gene names: 120609909

Gene position: 1347528-1346875 (Counterclockwise)

Preceding gene: 120609910

Following gene: 120609908

Centisome position: 25.17

GC content: 66.06

Gene sequence:

>654_bases
ATGCAGAAATTCACCCTCCACAAAGGCCTCGTGGCCCCCATGGACCGCGAGAACGTCGATACCGACGCCATCATCCCCAA
GCAGTTCCTGAAGTCGATCAAGAAGACGGGCTTCGGCCCCAACCTGTTCGACGAGTGGCGCTACCTGGACCAGCCGGGCC
AGCCCGGCGTGCCGGAATCGGCCCGCAAGCCCAACCCCGATTTCGTGCTGAACCAGCCGCGCTACGCGGGCGCCTCCATC
CTGCTGGCGCGCAAGAACTTCGGCTGCGGATCGAGCCGCGAGCATGCGCCCTGGGCGCTGGACCAGTACGGCTTCCGCGC
CATCATCGCGCCGAGCTTCGCCGACATCTTCTTCAACAACAGCTTCAAGAACGGCCTGCTGCCCATCGTGCTGCCCGAGG
CCACGGTCTCCCAGCTGTTCGACGAGGTGCATGCCTTCCCGGGCTACGAACTGACCGTCGATCTGGAGCGCCAGGTCATC
GTGCGCGCCCAGGGCGCCGAGATCCCGTTCGAGGTGAACGCCTTCCGCAAGTACTGCCTGCTCAACGGCTTCGACGACAT
CGGCCTCACGCTGCGCCAGGCCGACAAGATCCGCGCCTTCGAGGCGGAGCGGCTGGCCACCAAGCCCTGGCTCGCGCACA
CCATGCCGGCCTGA

Upstream 100 bases:

>100_bases
TGCAACACCGTCAAGGGCATGGGCCAGGACGTGCAGCGCGCCGGCAGCGCCATCGAGCGCGCCGCCAAGTAATCCGAACC
GAACCGCAACGGACACTGCC

Downstream 100 bases:

>100_bases
TTCCCCGAGACCTTCTTCGCAACCGCACCCAAGAGAACTACCAATGAAAATCGCAGTCCTGCCCGGCGACGGCATCGGCC
CCGAAATCGTGGCCGAGGCC

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MQKFTLHKGLVAPMDRENVDTDAIIPKQFLKSIKKTGFGPNLFDEWRYLDQPGQPGVPESARKPNPDFVLNQPRYAGASI
LLARKNFGCGSSREHAPWALDQYGFRAIIAPSFADIFFNNSFKNGLLPIVLPEATVSQLFDEVHAFPGYELTVDLERQVI
VRAQGAEIPFEVNAFRKYCLLNGFDDIGLTLRQADKIRAFEAERLATKPWLAHTMPA

Sequences:

>Translated_217_residues
MQKFTLHKGLVAPMDRENVDTDAIIPKQFLKSIKKTGFGPNLFDEWRYLDQPGQPGVPESARKPNPDFVLNQPRYAGASI
LLARKNFGCGSSREHAPWALDQYGFRAIIAPSFADIFFNNSFKNGLLPIVLPEATVSQLFDEVHAFPGYELTVDLERQVI
VRAQGAEIPFEVNAFRKYCLLNGFDDIGLTLRQADKIRAFEAERLATKPWLAHTMPA
>Mature_217_residues
MQKFTLHKGLVAPMDRENVDTDAIIPKQFLKSIKKTGFGPNLFDEWRYLDQPGQPGVPESARKPNPDFVLNQPRYAGASI
LLARKNFGCGSSREHAPWALDQYGFRAIIAPSFADIFFNNSFKNGLLPIVLPEATVSQLFDEVHAFPGYELTVDLERQVI
VRAQGAEIPFEVNAFRKYCLLNGFDDIGLTLRQADKIRAFEAERLATKPWLAHTMPA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=203, Percent_Identity=58.6206896551724, Blast_Score=245, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6321429, Length=207, Percent_Identity=48.792270531401, Blast_Score=189, Evalue=4e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_ACIAC (A1TLH5)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_969587.1
- STRING:   A1TLH5
- GeneID:   4665527
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_1222
- NMPDR:   fig|397945.5.peg.1071
- eggNOG:   COG0066
- HOGENOM:   HBG304838
- OMA:   DEISITM
- PhylomeDB:   A1TLH5
- ProtClustDB:   PRK01641
- BioCyc:   AAVE397945:AAVE_1222-MONOMER
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 24423; Mature: 24423

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKFTLHKGLVAPMDRENVDTDAIIPKQFLKSIKKTGFGPNLFDEWRYLDQPGQPGVPES
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCC
ARKPNPDFVLNQPRYAGASILLARKNFGCGSSREHAPWALDQYGFRAIIAPSFADIFFNN
CCCCCCCEEECCCCCCCEEEEEEECCCCCCCCCCCCCEEHHHHCCEEEECCHHHHHHCCC
SFKNGLLPIVLPEATVSQLFDEVHAFPGYELTVDLERQVIVRAQGAEIPFEVNAFRKYCL
CCCCCCCEEEECHHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCCCCCEEHHHHHHHHH
LNGFDDIGLTLRQADKIRAFEAERLATKPWLAHTMPA
HCCCCHHCHHHHHHHHHHHHHHHHHHCCCCHHCCCCC
>Mature Secondary Structure
MQKFTLHKGLVAPMDRENVDTDAIIPKQFLKSIKKTGFGPNLFDEWRYLDQPGQPGVPES
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCC
ARKPNPDFVLNQPRYAGASILLARKNFGCGSSREHAPWALDQYGFRAIIAPSFADIFFNN
CCCCCCCEEECCCCCCCEEEEEEECCCCCCCCCCCCCEEHHHHCCEEEECCHHHHHHCCC
SFKNGLLPIVLPEATVSQLFDEVHAFPGYELTVDLERQVIVRAQGAEIPFEVNAFRKYCL
CCCCCCCEEEECHHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCCCCCEEHHHHHHHHH
LNGFDDIGLTLRQADKIRAFEAERLATKPWLAHTMPA
HCCCCHHCHHHHHHHHHHHHHHHHHHCCCCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA