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The map label for this gene is leuC

Identifier: 120609910

GI number: 120609910

Start: 1347711

End: 1349132

Strand: Reverse

Name: leuC

Synonym: Aave_1223

Alternate gene names: 120609910

Gene position: 1349132-1347711 (Counterclockwise)

Preceding gene: 120609911

Following gene: 120609909

Centisome position: 25.2

GC content: 67.79

Gene sequence:

>1422_bases
ATGGGACGCACCCTCTACGACAAGATCTGGGACGAGCATGTCGTCCATACCGAAGAGGACGGCACGGCCATCCTCTACAT
CGACCGCCACCTGGTGCATGAAGTGACCAGCCCGCAGGCGTTCGAGGGCATCCGCCAGGCCGGCCGCAAGGTCTGGCGCG
TCAGCTCCATCGTCGCCACGGCCGACCACAACACGCCCACCACCGGCTGGGAGCGCGGCTACGACGGCATCGAGGACCCG
ATCAGCAAGGAACAGATCACCACGCTGGACAAGAACATCGCCGAAGTGGGCGCCGCCGCCTTCTTCCCGTTCATGAGCAA
GCGCCAGGGCATCGTGCACGTGATCGGCCCCGAGAACGGCGCCACCCTGCCCGGCATGACCGTGGTCTGCGGCGACAGCC
ATACCAGCACGCACGGCGCGTTCGGCGCCCTGGCGCACGGCATCGGCACCAGCGAGGTCGAACACGTGATGGCCACGCAG
ACCCTGCTGGCCAAGAAGGCGAAGAACATGCTGATCCGCGTGGAGGGCCAGGTCGCCCCCGGCGTGACGGCCAAGGACAT
CGTGCTGGCCATCATCGGCAAGATCGGCACGGCCGGCGGCACGGGCTACACCATCGAGTTCGCGGGCCCCGCCATCCGCG
CCCTGAGCATGGAAGGCCGCATGACGGTCTGCAACATGGCCATCGAGGCCGGCGCCCGCGCCGGCCTGGTGGCGGTGGAC
GACAAGACCATCGAGTACGTGAAGAACCGGCCGCTCTCCCCCACCGGTGTGGAATGGGATCAGGCCGTCGCCTACTGGAA
GACGCTGCATTCGGATGCCGACGCCCACTTCGACACCGTCGTCACCTTGAACGGCGCCGACATCGTTCCGCAGGTCACCT
GGGGCACCTCGCCCGAGATGGTGCTGGGCGTGGACGCCAGCGTGCCCGACCCGGACAAGGAAAAGGACCCGAACAAGCGC
AGCGCCATCGAGCGCGCGCTCACCTACATGGCACTGGAGCCCGGCAAGCCGCTGAACGACATCTTCGTGGACAAGGTGTT
CATCGGCAGTTGCACCAACAGCCGCATCGAGGACATGCGCGAAGCCGCTGCCGTGGTGAAAAAGCTGGGCCGGAAGGTGG
CACGGAACATCAAGGTGGCGATGGTGGTGCCCGGCTCGGGCCTGGTGAAGGAACAGGCCGAGCGCGAAGGCCTGGACCAG
GTCTTCAAGGCCGCGGGCTTCGAGTGGCGCGAGCCCGGCTGCTCCATGTGCCTGGCCATGAACGCCGACCGCCTGGAGCC
GGGCGAGCGCTGCGCGTCCACCAGCAACCGCAACTTCGAAGGTCGCCAGGGCGCGGGCGGGCGCACCCACCTCGTCAGCC
CCGCCATGGCGGCCGCCGCGGCCGTGCACGGGCACTTCGTGGACATCCGCAAGTTCGCCTGA

Upstream 100 bases:

>100_bases
CGGCTGCTGGCCGACCATCTGGCACCGCCGGAACGCAAGACCTCTTCCTAGAATCCACCCACCCATCCCCACCCTGAACC
AGCCCAAGAGAGAACACACC

Downstream 100 bases:

>100_bases
CCGCCACCAGCCCCTCCAGGAATCCGCCATGAAAACTTTCACCGCCCTGTTCGCGCTGGCCCTGGGCCTGCTGCTGGCCG
GCTGCAACACCGTCAAGGGC

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MGRTLYDKIWDEHVVHTEEDGTAILYIDRHLVHEVTSPQAFEGIRQAGRKVWRVSSIVATADHNTPTTGWERGYDGIEDP
ISKEQITTLDKNIAEVGAAAFFPFMSKRQGIVHVIGPENGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVMATQ
TLLAKKAKNMLIRVEGQVAPGVTAKDIVLAIIGKIGTAGGTGYTIEFAGPAIRALSMEGRMTVCNMAIEAGARAGLVAVD
DKTIEYVKNRPLSPTGVEWDQAVAYWKTLHSDADAHFDTVVTLNGADIVPQVTWGTSPEMVLGVDASVPDPDKEKDPNKR
SAIERALTYMALEPGKPLNDIFVDKVFIGSCTNSRIEDMREAAAVVKKLGRKVARNIKVAMVVPGSGLVKEQAEREGLDQ
VFKAAGFEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVHGHFVDIRKFA

Sequences:

>Translated_473_residues
MGRTLYDKIWDEHVVHTEEDGTAILYIDRHLVHEVTSPQAFEGIRQAGRKVWRVSSIVATADHNTPTTGWERGYDGIEDP
ISKEQITTLDKNIAEVGAAAFFPFMSKRQGIVHVIGPENGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVMATQ
TLLAKKAKNMLIRVEGQVAPGVTAKDIVLAIIGKIGTAGGTGYTIEFAGPAIRALSMEGRMTVCNMAIEAGARAGLVAVD
DKTIEYVKNRPLSPTGVEWDQAVAYWKTLHSDADAHFDTVVTLNGADIVPQVTWGTSPEMVLGVDASVPDPDKEKDPNKR
SAIERALTYMALEPGKPLNDIFVDKVFIGSCTNSRIEDMREAAAVVKKLGRKVARNIKVAMVVPGSGLVKEQAEREGLDQ
VFKAAGFEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVHGHFVDIRKFA
>Mature_472_residues
GRTLYDKIWDEHVVHTEEDGTAILYIDRHLVHEVTSPQAFEGIRQAGRKVWRVSSIVATADHNTPTTGWERGYDGIEDPI
SKEQITTLDKNIAEVGAAAFFPFMSKRQGIVHVIGPENGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVMATQT
LLAKKAKNMLIRVEGQVAPGVTAKDIVLAIIGKIGTAGGTGYTIEFAGPAIRALSMEGRMTVCNMAIEAGARAGLVAVDD
KTIEYVKNRPLSPTGVEWDQAVAYWKTLHSDADAHFDTVVTLNGADIVPQVTWGTSPEMVLGVDASVPDPDKEKDPNKRS
AIERALTYMALEPGKPLNDIFVDKVFIGSCTNSRIEDMREAAAVVKKLGRKVARNIKVAMVVPGSGLVKEQAEREGLDQV
FKAAGFEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVHGHFVDIRKFA

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=382, Percent_Identity=29.3193717277487, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI8659555, Length=437, Percent_Identity=26.7734553775744, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI41352693, Length=368, Percent_Identity=26.6304347826087, Blast_Score=92, Evalue=8e-19,
Organism=Escherichia coli, GI1786259, Length=472, Percent_Identity=63.771186440678, Blast_Score=586, Evalue=1e-169,
Organism=Escherichia coli, GI1787531, Length=365, Percent_Identity=26.3013698630137, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI87081781, Length=356, Percent_Identity=24.1573033707865, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI25149337, Length=381, Percent_Identity=29.1338582677165, Blast_Score=135, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI32564738, Length=381, Percent_Identity=29.1338582677165, Blast_Score=134, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI25149342, Length=318, Percent_Identity=28.3018867924528, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17568399, Length=438, Percent_Identity=27.6255707762557, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6321429, Length=474, Percent_Identity=63.9240506329114, Blast_Score=612, Evalue=1e-176,
Organism=Saccharomyces cerevisiae, GI6320440, Length=465, Percent_Identity=26.8817204301075, Blast_Score=139, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6323335, Length=369, Percent_Identity=29.5392953929539, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6322261, Length=368, Percent_Identity=26.3586956521739, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI281365315, Length=458, Percent_Identity=27.5109170305677, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI17864292, Length=458, Percent_Identity=27.5109170305677, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI161076999, Length=396, Percent_Identity=28.2828282828283, Blast_Score=119, Evalue=7e-27,
Organism=Drosophila melanogaster, GI28571643, Length=369, Percent_Identity=26.8292682926829, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24645686, Length=441, Percent_Identity=28.3446712018141, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI17137564, Length=375, Percent_Identity=28, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_ACIAC (A1TLH6)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_969588.1
- STRING:   A1TLH6
- GeneID:   4665149
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_1223
- NMPDR:   fig|397945.5.peg.1073
- eggNOG:   COG0065
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- PhylomeDB:   A1TLH6
- ProtClustDB:   PRK05478
- BioCyc:   AAVE397945:AAVE_1223-MONOMER
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 50848; Mature: 50717

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRTLYDKIWDEHVVHTEEDGTAILYIDRHLVHEVTSPQAFEGIRQAGRKVWRVSSIVAT
CCCCHHHHHHHHHCEEECCCCCEEEEECHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEE
ADHNTPTTGWERGYDGIEDPISKEQITTLDKNIAEVGAAAFFPFMSKRQGIVHVIGPENG
CCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
ATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVMATQTLLAKKAKNMLIRVEGQVAP
CCCCCEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCC
GVTAKDIVLAIIGKIGTAGGTGYTIEFAGPAIRALSMEGRMTVCNMAIEAGARAGLVAVD
CCCHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEECCCCCHHHHHHHHCCCCCCEEEEC
DKTIEYVKNRPLSPTGVEWDQAVAYWKTLHSDADAHFDTVVTLNGADIVPQVTWGTSPEM
CHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEECCCCCCE
VLGVDASVPDPDKEKDPNKRSAIERALTYMALEPGKPLNDIFVDKVFIGSCTNSRIEDMR
EEEECCCCCCCCCCCCCCHHHHHHHHHHHHEECCCCCHHHHHHHHHEECCCCCHHHHHHH
EAAAVVKKLGRKVARNIKVAMVVPGSGLVKEQAEREGLDQVFKAAGFEWREPGCSMCLAM
HHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEC
NADRLEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVHGHFVDIRKFA
CCCCCCCHHHHHCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHEEEHHHCC
>Mature Secondary Structure 
GRTLYDKIWDEHVVHTEEDGTAILYIDRHLVHEVTSPQAFEGIRQAGRKVWRVSSIVAT
CCCHHHHHHHHHCEEECCCCCEEEEECHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEE
ADHNTPTTGWERGYDGIEDPISKEQITTLDKNIAEVGAAAFFPFMSKRQGIVHVIGPENG
CCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
ATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVMATQTLLAKKAKNMLIRVEGQVAP
CCCCCEEEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCC
GVTAKDIVLAIIGKIGTAGGTGYTIEFAGPAIRALSMEGRMTVCNMAIEAGARAGLVAVD
CCCHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEECCCCCHHHHHHHHCCCCCCEEEEC
DKTIEYVKNRPLSPTGVEWDQAVAYWKTLHSDADAHFDTVVTLNGADIVPQVTWGTSPEM
CHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEECCCCCCE
VLGVDASVPDPDKEKDPNKRSAIERALTYMALEPGKPLNDIFVDKVFIGSCTNSRIEDMR
EEEECCCCCCCCCCCCCCHHHHHHHHHHHHEECCCCCHHHHHHHHHEECCCCCHHHHHHH
EAAAVVKKLGRKVARNIKVAMVVPGSGLVKEQAEREGLDQVFKAAGFEWREPGCSMCLAM
HHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEC
NADRLEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAAAAAVHGHFVDIRKFA
CCCCCCCHHHHHCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHEEEHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA