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The map label for this gene is leuB [H]

Identifier: 120609908

GI number: 120609908

Start: 1345758

End: 1346831

Strand: Reverse

Name: leuB [H]

Synonym: Aave_1221

Alternate gene names: 120609908

Gene position: 1346831-1345758 (Counterclockwise)

Preceding gene: 120609909

Following gene: 120609907

Centisome position: 25.16

GC content: 67.78

Gene sequence:

>1074_bases
ATGAAAATCGCAGTCCTGCCCGGCGACGGCATCGGCCCCGAAATCGTGGCCGAGGCCGTGAAGGTGCTCGAAACGCTCGA
CCTTCCCTTCGAGATGGAATCCGCCCTGGTCGGCGGCGCGGCCTACGAGGCCCACGGCCACCCCCTGCCCGAGTCCACGC
TGACGCTCGCCCGGGAAGCCGATGCCGTGCTGTTCGGCGCCGTGGGCGACTGGAAGTACGACAAGCTCGACCGCCCGCTG
CGTCCCGAGCAGGCCATCCTGGGCCTGCGCAAGAACCTCGGCCTGTTCGCCAACTTCCGCCCGGCGATCTGCTACGAGCA
GCTCACGCACGCCTCCAGCCTCAAGCCCGAACTCGTCGCGGGCCTGGACATCCTCATCATCCGGGAGCTGACGGGCGACA
TCTACTTCGGCCAGCCGCGCGGCCGCCGCACGGCCACCGACGGGCATTTCCCCGGCGCCGAGGAAGCCTTCGACACCATG
CGCTACAGCCGCCCGGAGATCGAGCGCATCGCGCACGTCGCCTTCCAGGCCGCGCGCAAGCGCAGCAGGAAGGTCACGAG
CGTGGACAAGGCCAACGTGCTGGAAACCTTCCAGTTCTGGAAGGACGTGGTCACCGACGTGCACAAGGAATACCCCGACG
TGGAGCTGGAGCACATGTACGTGGACAACGCCGCCATGCAGCTGGTGAAGAAGCCCAAGGCCTTCGACGTGGTGGTGACG
GGCAACATGTTCGGCGACATTCTTTCGGACGAGGCCTCCATGCTCACGGGTTCCATCGGCATGCTGCCTTCGGCCAGCCT
GAACAGCAGGAACCAGGGCCTGTACGAGCCCAGCCACGGCAGCGCGCCCGACATCGCCGGCCAGGGCATCGCCAACCCGC
TGGCCACCATCCTGTCGGCCGCCATGATGCTGCGCTTCAGCCTGAACCAGGAAGCCGCCGCCCAGCGCATCGAAGCCGCC
GTGCAGAACGTGCTGGCCCAGGGCCTGCGCACGCCCGACATCTGGAGCGAGGGCACGACCAAGGTCGGCACGGCGCAGAT
GGGCGATGCGGTGGTGAAGGCCCTGGCGCGCTGA

Upstream 100 bases:

>100_bases
TTCGAGGCGGAGCGGCTGGCCACCAAGCCCTGGCTCGCGCACACCATGCCGGCCTGATTCCCCGAGACCTTCTTCGCAAC
CGCACCCAAGAGAACTACCA

Downstream 100 bases:

>100_bases
CACCTTACCCGTTCCACTTCCCGACCAGCGGCCCACAAGGGCCGCTTTTTTTCGGCTGCCGCCCGGCGCATCATGGGTCC
GGATGCGGCCAGACGGCCAG

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MKIAVLPGDGIGPEIVAEAVKVLETLDLPFEMESALVGGAAYEAHGHPLPESTLTLAREADAVLFGAVGDWKYDKLDRPL
RPEQAILGLRKNLGLFANFRPAICYEQLTHASSLKPELVAGLDILIIRELTGDIYFGQPRGRRTATDGHFPGAEEAFDTM
RYSRPEIERIAHVAFQAARKRSRKVTSVDKANVLETFQFWKDVVTDVHKEYPDVELEHMYVDNAAMQLVKKPKAFDVVVT
GNMFGDILSDEASMLTGSIGMLPSASLNSRNQGLYEPSHGSAPDIAGQGIANPLATILSAAMMLRFSLNQEAAAQRIEAA
VQNVLAQGLRTPDIWSEGTTKVGTAQMGDAVVKALAR

Sequences:

>Translated_357_residues
MKIAVLPGDGIGPEIVAEAVKVLETLDLPFEMESALVGGAAYEAHGHPLPESTLTLAREADAVLFGAVGDWKYDKLDRPL
RPEQAILGLRKNLGLFANFRPAICYEQLTHASSLKPELVAGLDILIIRELTGDIYFGQPRGRRTATDGHFPGAEEAFDTM
RYSRPEIERIAHVAFQAARKRSRKVTSVDKANVLETFQFWKDVVTDVHKEYPDVELEHMYVDNAAMQLVKKPKAFDVVVT
GNMFGDILSDEASMLTGSIGMLPSASLNSRNQGLYEPSHGSAPDIAGQGIANPLATILSAAMMLRFSLNQEAAAQRIEAA
VQNVLAQGLRTPDIWSEGTTKVGTAQMGDAVVKALAR
>Mature_357_residues
MKIAVLPGDGIGPEIVAEAVKVLETLDLPFEMESALVGGAAYEAHGHPLPESTLTLAREADAVLFGAVGDWKYDKLDRPL
RPEQAILGLRKNLGLFANFRPAICYEQLTHASSLKPELVAGLDILIIRELTGDIYFGQPRGRRTATDGHFPGAEEAFDTM
RYSRPEIERIAHVAFQAARKRSRKVTSVDKANVLETFQFWKDVVTDVHKEYPDVELEHMYVDNAAMQLVKKPKAFDVVVT
GNMFGDILSDEASMLTGSIGMLPSASLNSRNQGLYEPSHGSAPDIAGQGIANPLATILSAAMMLRFSLNQEAAAQRIEAA
VQNVLAQGLRTPDIWSEGTTKVGTAQMGDAVVKALAR

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=339, Percent_Identity=32.4483775811209, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI4758582, Length=344, Percent_Identity=29.0697674418605, Blast_Score=121, Evalue=8e-28,
Organism=Homo sapiens, GI28178821, Length=347, Percent_Identity=29.1066282420749, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI28178816, Length=362, Percent_Identity=29.0055248618785, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI28178838, Length=339, Percent_Identity=28.3185840707965, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI28178819, Length=181, Percent_Identity=34.8066298342541, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI87081683, Length=360, Percent_Identity=53.3333333333333, Blast_Score=369, Evalue=1e-103,
Organism=Escherichia coli, GI1788101, Length=372, Percent_Identity=35.2150537634409, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1787381, Length=403, Percent_Identity=25.3101736972705, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71986051, Length=349, Percent_Identity=32.378223495702, Blast_Score=155, Evalue=4e-38,
Organism=Caenorhabditis elegans, GI17550882, Length=360, Percent_Identity=31.1111111111111, Blast_Score=144, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI25144293, Length=346, Percent_Identity=33.2369942196532, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17505779, Length=336, Percent_Identity=32.1428571428571, Blast_Score=132, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6319830, Length=371, Percent_Identity=45.0134770889488, Blast_Score=276, Evalue=4e-75,
Organism=Saccharomyces cerevisiae, GI6322097, Length=359, Percent_Identity=35.3760445682451, Blast_Score=181, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6324291, Length=344, Percent_Identity=32.5581395348837, Blast_Score=147, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6324709, Length=362, Percent_Identity=32.8729281767956, Blast_Score=137, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24643268, Length=341, Percent_Identity=32.258064516129, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24643270, Length=341, Percent_Identity=32.258064516129, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI161078635, Length=347, Percent_Identity=27.6657060518732, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI161078637, Length=347, Percent_Identity=27.6657060518732, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI161078633, Length=347, Percent_Identity=27.6657060518732, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24650122, Length=347, Percent_Identity=27.6657060518732, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24661184, Length=372, Percent_Identity=27.6881720430108, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI161078639, Length=345, Percent_Identity=27.8260869565217, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI281362242, Length=353, Percent_Identity=28.6118980169972, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24648872, Length=353, Percent_Identity=28.6118980169972, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI20130355, Length=170, Percent_Identity=31.1764705882353, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 38782; Mature: 38782

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAVLPGDGIGPEIVAEAVKVLETLDLPFEMESALVGGAAYEAHGHPLPESTLTLAREA
CEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHC
DAVLFGAVGDWKYDKLDRPLRPEQAILGLRKNLGLFANFRPAICYEQLTHASSLKPELVA
CEEEEECCCCCCHHHCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHH
GLDILIIRELTGDIYFGQPRGRRTATDGHFPGAEEAFDTMRYSRPEIERIAHVAFQAARK
CCHHEEEEECCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RSRKVTSVDKANVLETFQFWKDVVTDVHKEYPDVELEHMYVDNAAMQLVKKPKAFDVVVT
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHCCCCEEEEEE
GNMFGDILSDEASMLTGSIGMLPSASLNSRNQGLYEPSHGSAPDIAGQGIANPLATILSA
CCHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
AMMLRFSLNQEAAAQRIEAAVQNVLAQGLRTPDIWSEGTTKVGTAQMGDAVVKALAR
HHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKIAVLPGDGIGPEIVAEAVKVLETLDLPFEMESALVGGAAYEAHGHPLPESTLTLAREA
CEEEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHC
DAVLFGAVGDWKYDKLDRPLRPEQAILGLRKNLGLFANFRPAICYEQLTHASSLKPELVA
CEEEEECCCCCCHHHCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHH
GLDILIIRELTGDIYFGQPRGRRTATDGHFPGAEEAFDTMRYSRPEIERIAHVAFQAARK
CCHHEEEEECCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RSRKVTSVDKANVLETFQFWKDVVTDVHKEYPDVELEHMYVDNAAMQLVKKPKAFDVVVT
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHCCCCEEEEEE
GNMFGDILSDEASMLTGSIGMLPSASLNSRNQGLYEPSHGSAPDIAGQGIANPLATILSA
CCHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
AMMLRFSLNQEAAAQRIEAAVQNVLAQGLRTPDIWSEGTTKVGTAQMGDAVVKALAR
HHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA