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The map label for this gene is hisF

Identifier: 120609740

GI number: 120609740

Start: 1152162

End: 1152962

Strand: Direct

Name: hisF

Synonym: Aave_1047

Alternate gene names: 120609740

Gene position: 1152162-1152962 (Clockwise)

Preceding gene: 120609739

Following gene: 120609741

Centisome position: 21.52

GC content: 71.54

Gene sequence:

>801_bases
ATGCTGGCCAAACGCATCATTCCCTGTCTGGACGTCACCGGAGGCCGCGTCGTGAAGGGCGTGAACTTCCTGGAGTTGCG
CGATGCCGGCGACCCGGTGGAGATCGCCGCGCGCTACAACGGCCAGGGTGCCGACGAGCTCACTTTCCTGGACATCACCG
CCACCAGCGACGGGCGCGACCTGATCCTGCCCATCATCGAGGCCGTGGCCAGCCAGGTGTTCATCCCCCTCACGGTGGGC
GGCGGCGTGCGCACCGTGGAGGACGTGCGGCGCCTGCTGAACGCGGGCGCGGACAAGACCAGTTTCAATTCCGCCGCCAT
CGCCAACCCGGAGGTGATCGACGCCGCCTCCGCGAAATACGGCGCGCAGTGCATCGTGGTGGCCATCGACGCCAAGCGCC
GCACCCCCGAGGAGGCCGCGCGCCCCGGCCCGGACGGCGCGCCCCGCGGCGAAGGCTGGGACGTGTACAGCCATGGCGGG
CGCAAGAACACCGGCCTGGACGCCGTGCAGTGGGCCGCCGAGATGGCGCGCCGCGGCGCGGGCGAGATCCTGCTCACCAG
CATGGACCGGGACGGCACCAAGTCCGGCTTCGACCTGGCCCTGACCCGCGCCGTGAGCGACGCGGTGAGCGTGCCGGTGA
TCGCCTCGGGCGGCGTGGGCGGCCTCGACGACCTGGCCGACGGCGTGCAGCAGGGCGGGGCGGACGCGGTCCTGGCCGCC
AGCATCTTCCACTATGGCGAATACACGGTGGCGCAGGCCAAGGAACGCATGGCCGCACGGGGCATTCCCGTGCGCCTCTA
G

Upstream 100 bases:

>100_bases
CTGGCAGCGGTTCGAAGTGCTGCGCTGACGGCTCCCTGGGTTCCGTTCCCCGTTCCTCTTTTCTTCCTTACTTTCCCTCT
CTCTCTCGCAGGACCCGCCC

Downstream 100 bases:

>100_bases
GGGCATTCCCCCGGAGGACATCGTGGTTCATGCTGCAGTGCAACAAAAAAGCGGAGAATGGGGCGATGAGCTGGCTCGAT
GAAGTGAAATGGGATGCGCA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MLAKRIIPCLDVTGGRVVKGVNFLELRDAGDPVEIAARYNGQGADELTFLDITATSDGRDLILPIIEAVASQVFIPLTVG
GGVRTVEDVRRLLNAGADKTSFNSAAIANPEVIDAASAKYGAQCIVVAIDAKRRTPEEAARPGPDGAPRGEGWDVYSHGG
RKNTGLDAVQWAAEMARRGAGEILLTSMDRDGTKSGFDLALTRAVSDAVSVPVIASGGVGGLDDLADGVQQGGADAVLAA
SIFHYGEYTVAQAKERMAARGIPVRL

Sequences:

>Translated_266_residues
MLAKRIIPCLDVTGGRVVKGVNFLELRDAGDPVEIAARYNGQGADELTFLDITATSDGRDLILPIIEAVASQVFIPLTVG
GGVRTVEDVRRLLNAGADKTSFNSAAIANPEVIDAASAKYGAQCIVVAIDAKRRTPEEAARPGPDGAPRGEGWDVYSHGG
RKNTGLDAVQWAAEMARRGAGEILLTSMDRDGTKSGFDLALTRAVSDAVSVPVIASGGVGGLDDLADGVQQGGADAVLAA
SIFHYGEYTVAQAKERMAARGIPVRL
>Mature_266_residues
MLAKRIIPCLDVTGGRVVKGVNFLELRDAGDPVEIAARYNGQGADELTFLDITATSDGRDLILPIIEAVASQVFIPLTVG
GGVRTVEDVRRLLNAGADKTSFNSAAIANPEVIDAASAKYGAQCIVVAIDAKRRTPEEAARPGPDGAPRGEGWDVYSHGG
RKNTGLDAVQWAAEMARRGAGEILLTSMDRDGTKSGFDLALTRAVSDAVSVPVIASGGVGGLDDLADGVQQGGADAVLAA
SIFHYGEYTVAQAKERMAARGIPVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=266, Percent_Identity=40.2255639097744, Blast_Score=197, Evalue=8e-52,
Organism=Escherichia coli, GI87082028, Length=252, Percent_Identity=30.1587301587302, Blast_Score=97, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6319725, Length=315, Percent_Identity=34.9206349206349, Blast_Score=160, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_ACIAC (A1TL06)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_969418.1
- ProteinModelPortal:   A1TL06
- SMR:   A1TL06
- STRING:   A1TL06
- GeneID:   4669508
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_1047
- NMPDR:   fig|397945.5.peg.910
- eggNOG:   COG0107
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- PhylomeDB:   A1TL06
- ProtClustDB:   PRK02083
- BioCyc:   AAVE397945:AAVE_1047-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27685; Mature: 27685

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVTGGRVVKGVNFLELRDAGDPVEIAARYNGQGADELTFLDITATSDGRD
CCHHHCCCHHCCCCCEEECCCCEEEECCCCCCEEEEEEECCCCCCCEEEEEEEECCCCCC
LILPIIEAVASQVFIPLTVGGGVRTVEDVRRLLNAGADKTSFNSAAIANPEVIDAASAKY
HHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCCCCCC
GAQCIVVAIDAKRRTPEEAARPGPDGAPRGEGWDVYSHGGRKNTGLDAVQWAAEMARRGA
CCEEEEEEEECCCCCHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHCCC
GEILLTSMDRDGTKSGFDLALTRAVSDAVSVPVIASGGVGGLDDLADGVQQGGADAVLAA
CCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCCCHHHHH
SIFHYGEYTVAQAKERMAARGIPVRL
HHHHCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLAKRIIPCLDVTGGRVVKGVNFLELRDAGDPVEIAARYNGQGADELTFLDITATSDGRD
CCHHHCCCHHCCCCCEEECCCCEEEECCCCCCEEEEEEECCCCCCCEEEEEEEECCCCCC
LILPIIEAVASQVFIPLTVGGGVRTVEDVRRLLNAGADKTSFNSAAIANPEVIDAASAKY
HHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCCCCCCC
GAQCIVVAIDAKRRTPEEAARPGPDGAPRGEGWDVYSHGGRKNTGLDAVQWAAEMARRGA
CCEEEEEEEECCCCCHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHCCC
GEILLTSMDRDGTKSGFDLALTRAVSDAVSVPVIASGGVGGLDDLADGVQQGGADAVLAA
CCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCCCHHHHH
SIFHYGEYTVAQAKERMAARGIPVRL
HHHHCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA