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The map label for this gene is mlaE [H]

Identifier: 120609708

GI number: 120609708

Start: 1115828

End: 1116610

Strand: Direct

Name: mlaE [H]

Synonym: Aave_1014

Alternate gene names: 120609708

Gene position: 1115828-1116610 (Clockwise)

Preceding gene: 120609707

Following gene: 120609709

Centisome position: 20.85

GC content: 69.6

Gene sequence:

>783_bases
ATGAGCTGGTGGAAACCTTCCGATGTCGGCTTCGCCACGCGCAGCCTGCTGGCGGACATGGGCCGCGCGGCGCGGCTGTT
CGTGCGTCTGGCCGGCCTGATCGGCCCGACCCTGCAGCGCTTCGGGCTGGTGCGCGACCAGTTGCACTTCCTGGGCAACT
ATTCGCTGTCCATCATTTCCGTGTCGGGCCTGTTCGTGGGCTTCGTGCTGGGCCTGCAGGGCTACTACACGCTGCAGCGC
TACGGCTCCGCGCAGGCGCTGGGCCTGCTCGTGGCGCTGTCGCTGGTGCGCGAGCTCGGCCCCGTCGTCTCCGCGCTGCT
GTTCGCGGGCCGCGCTGGCACTTCGCTCACGGCGGAGATCGGCCTGATGCGCGCGGGCGAGCAGCTCTCGGCCATGGAGA
TGATGGCCGTGGACCCGGTGCGCCGCATCCTGGCCCCGCGCTTTTGGGCCGGGGTGATCGCGATGCCGCTGCTGGCCGCG
GTGTTCAGCGCGGTGGGCGTGATCGGCGGCTGGATCGTCGGCGTGGTCATGCTCGGCGTGGACGGCGGTGCCTTCTGGGG
CCAGATGCAGAGCGGGGTGGACGTGTGGGCGGACGTGGGCAACGGCGTGCTCAAGAGCTTCGTCTTCGGCGTCACGGTCA
CTTTCATCGCCCTGCTGCAGGGCTACATCGCCAAGCCCACGCCCGAGGGCGTGTCCCGCGCCACCACCCGCACCGTGGTG
ATGGCCTCGCTCACGGTGCTGGGGCTGGATTTCCTGCTCACGGCCTGGATGTTCAGCATCTGA

Upstream 100 bases:

>100_bases
TCGTGCATGCGCTGCCCACCGGGCCGGTGCCGTTCCATTACCCCGGCCCTTCGGTGGCCGACGATTTCGGGCCGCTGGCC
GGCCCGGGCGGAGGCGCGCG

Downstream 100 bases:

>100_bases
CGCGGCAGCGCCCAAGAACCCTTCAGAGAGAAAACACCATGCAGCATTCCAAGAACGACGTCTGGGTCGGCCTCTTCGTC
CTCCTCGGCGGTGCGGCCCT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MSWWKPSDVGFATRSLLADMGRAARLFVRLAGLIGPTLQRFGLVRDQLHFLGNYSLSIISVSGLFVGFVLGLQGYYTLQR
YGSAQALGLLVALSLVRELGPVVSALLFAGRAGTSLTAEIGLMRAGEQLSAMEMMAVDPVRRILAPRFWAGVIAMPLLAA
VFSAVGVIGGWIVGVVMLGVDGGAFWGQMQSGVDVWADVGNGVLKSFVFGVTVTFIALLQGYIAKPTPEGVSRATTRTVV
MASLTVLGLDFLLTAWMFSI

Sequences:

>Translated_260_residues
MSWWKPSDVGFATRSLLADMGRAARLFVRLAGLIGPTLQRFGLVRDQLHFLGNYSLSIISVSGLFVGFVLGLQGYYTLQR
YGSAQALGLLVALSLVRELGPVVSALLFAGRAGTSLTAEIGLMRAGEQLSAMEMMAVDPVRRILAPRFWAGVIAMPLLAA
VFSAVGVIGGWIVGVVMLGVDGGAFWGQMQSGVDVWADVGNGVLKSFVFGVTVTFIALLQGYIAKPTPEGVSRATTRTVV
MASLTVLGLDFLLTAWMFSI
>Mature_259_residues
SWWKPSDVGFATRSLLADMGRAARLFVRLAGLIGPTLQRFGLVRDQLHFLGNYSLSIISVSGLFVGFVLGLQGYYTLQRY
GSAQALGLLVALSLVRELGPVVSALLFAGRAGTSLTAEIGLMRAGEQLSAMEMMAVDPVRRILAPRFWAGVIAMPLLAAV
FSAVGVIGGWIVGVVMLGVDGGAFWGQMQSGVDVWADVGNGVLKSFVFGVTVTFIALLQGYIAKPTPEGVSRATTRTVVM
ASLTVLGLDFLLTAWMFSI

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=245, Percent_Identity=60.4081632653061, Blast_Score=262, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 27637; Mature: 27506

Theoretical pI: Translated: 10.33; Mature: 10.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SWWKPSDVGFATRSLLADMGRAARLFVRLAGLIGPTLQRFGLVRDQLHFLGNYSLSIIS
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
VSGLFVGFVLGLQGYYTLQRYGSAQALGLLVALSLVRELGPVVSALLFAGRAGTSLTAEI
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
GLMRAGEQLSAMEMMAVDPVRRILAPRFWAGVIAMPLLAAVFSAVGVIGGWIVGVVMLGV
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DGGAFWGQMQSGVDVWADVGNGVLKSFVFGVTVTFIALLQGYIAKPTPEGVSRATTRTVV
CCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
MASLTVLGLDFLLTAWMFSI
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]