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The map label for this gene is mrcA [H]

Identifier: 120609689

GI number: 120609689

Start: 1090457

End: 1092850

Strand: Reverse

Name: mrcA [H]

Synonym: Aave_0995

Alternate gene names: 120609689

Gene position: 1092850-1090457 (Counterclockwise)

Preceding gene: 120609728

Following gene: 120609687

Centisome position: 20.42

GC content: 65.46

Gene sequence:

>2394_bases
ATGCCGCCTCCCCCGACCGACAAGACCGCTCGCGAGAGCGCTTCCGCCCGTCCCACGTGGCTCCGCTGGCTGCTCAGGAG
CTTGGCCTGGCTCTTCGCGATCGCGGCCGCGGGCGCGCTGGGCCTCGCCCTGCTGATCGCCGTCGCGCTGGCGGTGGCGT
ACCCCAACCTGCCGGACATCTCCGACCTCGCCGACTACCGGCCCAAGCTGCCTCTGCGCGTCTATTCCGCCGAAGGGGCC
CTGCTCGGCGAGTTCGGCGAGGAGCGCCGCAATCTCACGCCCATCCAGGACATCCCCAAGGTCATGACCAATGCGGTGCT
GGCCATCGAGGATGCCCGCTTCTTCCAGCATGGCGGCGTGGACTACAAGGGCATGGTCCGCGCGGCCCTGGCCAATCTGG
GCCGCGTGAAGAGTCAGGGAGCCTCCACCATCACCATGCAGGTGGCAAGAAACGTTTATCTGTCGTCCGAGAAAACAATT
ACTCGCAAAATTTACGAAATATTACTAACTTTCAAACTCGAGCATCTGTTGACCAAAAATCAGATTCTTGAGATTTACAT
GAACCAGATTTACCTGGGCAACCGGGCCTATGGTTTCGCTGCCGCATCGGAGGCGTATTTCGGAAAACCGCTGAAATCCA
TCAGCATCGCCGAGGCGGCGATGCTGGCGGGCCTGCCCAAGGCACCTTCCGCGTACAACCCCATCAGCAACCCCAAACGC
GCCCGCGTGCGCCAGCTCTACATCATCGACCGCATGGTGGAGAACGGCTTCATCACGCCCGAGGAGGGCGCGGCCGCCAA
GCAGGAAGACCTGAAGATCCGTTCCGGCGGCCCGGACAATACCCGCATCCATGCCGAATACGTGGCCGAGATGGCGCGCC
AGCTCATCTTCGCGCAATATGGCAGCGAAGCCTATACGCGCGGCCTGAATGTCTATACCACCCTCACCGCGGGGGACCAG
GAAGCGGCGTACCAGGCTTTGCGCAAGGGCATCATGGATTACGAGCGCCGCCAGCATTACCGCGGGCCGGAGAAATTCGT
CGCCCTGCCCACCGATCCCCAGGAAGCCGAGGACGTCATCGACGACACGCTCGCGGGCCATCCCGACAATGGCGACGTGC
TCTCGGCGGTGGTACTGGAAGCTTCTCCGAAGAAGATCGTGGCGGCGCGCGCGAACGGCGAGCCCATCGAGATCACCGGC
GACGGCCTCAAGCCCGCGCAGTCCGGGCTCAGTGACAAGGCGCCGCCCAACATCCGCATCCGCCGCGGCGCGGTGATCCG
CATCATCCAGACGCCCAAGAAGACCTGGGACATCACGCAACTGCCCGAGGTCGAAGGCGCTTTCGTGGCCGTCGACCCCC
GCAACGGCGCGATCAAGGCGCTCGTGGGCGGCTTCGATTTCGACAAGAACAAGTTCAACCACGTCACCCAGGCGTGGCGG
CAGCCGGGCTCGAGCTTCAAGCCCTTCATCTATTCCGCGGCGCTCGAGAAGGGGTTCACGCCTGCGACGGTGGTGAACGA
CGCCCCGCTGTTCTTCAGCGCAGGCACCACGGGCGGGCAGCCCTGGGAACCCAAGAACTACGACGGCAAGTACGACGGCC
CCATGACCCTGCGCACCGCGCTGGCCAAGTCCAAAAACGTCGTGTCCATCCGCATCCTCCAGGCCGTGGGCCCGAAGACC
GCGCAGGACTGGGTCACGCGATTCGGCTTCGACGCGGACAAGCACCCTGCCTACCTGACGATGGCGCTGGGCGCCGGCTC
CGTGACGCCGATGCAGATGGTGTCCGCGTATTCGGTGTTCGCCAACGGCGGCTACCGCGTGAACCCCTATCTCATCACCC
GGGTGACGGACCACAAGGGACGCATCCTGTCCAACTTCGAGCCGCCGGCGACCACCGACAATCCGCGCGCCATCGACGCG
CGCAATGCGTTCATCATGGACAGCCTGTTGCAGGAGGTGACGCGTTCGGGCACGGCCGCCCGCGCGCAGGCGACGCTCAA
GCGTCCGGACGTCTATGGCAAGACCGGCACCACCAACGATGCCATGGATGCGTGGTTCGCGGGCTTCCAGCCCACCATCG
CGGCGGTCACCTGGATCGGCTATGACACCCCGCGCAACCTGGGCAGCCGAGAAACCGGCGGCGGCCTGAGCCTGCCGGTC
TGGATCAGCTTCATGGAGCGCGCCCTGAAGGGGGTGCCCGTGGCCGAGCCCACGGTGCCACCCGGCGTGGTGAATGTCGG
CGGCGAATGGTTCTACGAGGAATTCGCCCGCAATGCCGGCGTGGCGAGCGTGGGCATGGAAGACCATCCCGCCGCCGCGG
TGCCGGGCGCCCCGCCGCAGGCCCCTCCCCCGGCGGAAGAGCGCAACCGCATCCTGGACCTGTTCAGGAACTGA

Upstream 100 bases:

>100_bases
GCGCTGCCGCGTCGGGCGCGGGCTCCGGGGGACGACCCTGAAAAAACGTGATGCAACGTTCGATTTTTTATAATGCCCGG
TTGATTTGCACTGGACAGAT

Downstream 100 bases:

>100_bases
ACCCGCCCCCCGCCTTCCCGCCAGTGCAGTGCGGCGGGCGATCGAGGCACCCCGGCGCCTGCCGCGGTGCCTCTTCTTTT
CTGGCGAGCAGCCCCTGGTT

Product: 1A family penicillin-binding protein

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 797; Mature: 796

Protein sequence:

>797_residues
MPPPPTDKTARESASARPTWLRWLLRSLAWLFAIAAAGALGLALLIAVALAVAYPNLPDISDLADYRPKLPLRVYSAEGA
LLGEFGEERRNLTPIQDIPKVMTNAVLAIEDARFFQHGGVDYKGMVRAALANLGRVKSQGASTITMQVARNVYLSSEKTI
TRKIYEILLTFKLEHLLTKNQILEIYMNQIYLGNRAYGFAAASEAYFGKPLKSISIAEAAMLAGLPKAPSAYNPISNPKR
ARVRQLYIIDRMVENGFITPEEGAAAKQEDLKIRSGGPDNTRIHAEYVAEMARQLIFAQYGSEAYTRGLNVYTTLTAGDQ
EAAYQALRKGIMDYERRQHYRGPEKFVALPTDPQEAEDVIDDTLAGHPDNGDVLSAVVLEASPKKIVAARANGEPIEITG
DGLKPAQSGLSDKAPPNIRIRRGAVIRIIQTPKKTWDITQLPEVEGAFVAVDPRNGAIKALVGGFDFDKNKFNHVTQAWR
QPGSSFKPFIYSAALEKGFTPATVVNDAPLFFSAGTTGGQPWEPKNYDGKYDGPMTLRTALAKSKNVVSIRILQAVGPKT
AQDWVTRFGFDADKHPAYLTMALGAGSVTPMQMVSAYSVFANGGYRVNPYLITRVTDHKGRILSNFEPPATTDNPRAIDA
RNAFIMDSLLQEVTRSGTAARAQATLKRPDVYGKTGTTNDAMDAWFAGFQPTIAAVTWIGYDTPRNLGSRETGGGLSLPV
WISFMERALKGVPVAEPTVPPGVVNVGGEWFYEEFARNAGVASVGMEDHPAAAVPGAPPQAPPPAEERNRILDLFRN

Sequences:

>Translated_797_residues
MPPPPTDKTARESASARPTWLRWLLRSLAWLFAIAAAGALGLALLIAVALAVAYPNLPDISDLADYRPKLPLRVYSAEGA
LLGEFGEERRNLTPIQDIPKVMTNAVLAIEDARFFQHGGVDYKGMVRAALANLGRVKSQGASTITMQVARNVYLSSEKTI
TRKIYEILLTFKLEHLLTKNQILEIYMNQIYLGNRAYGFAAASEAYFGKPLKSISIAEAAMLAGLPKAPSAYNPISNPKR
ARVRQLYIIDRMVENGFITPEEGAAAKQEDLKIRSGGPDNTRIHAEYVAEMARQLIFAQYGSEAYTRGLNVYTTLTAGDQ
EAAYQALRKGIMDYERRQHYRGPEKFVALPTDPQEAEDVIDDTLAGHPDNGDVLSAVVLEASPKKIVAARANGEPIEITG
DGLKPAQSGLSDKAPPNIRIRRGAVIRIIQTPKKTWDITQLPEVEGAFVAVDPRNGAIKALVGGFDFDKNKFNHVTQAWR
QPGSSFKPFIYSAALEKGFTPATVVNDAPLFFSAGTTGGQPWEPKNYDGKYDGPMTLRTALAKSKNVVSIRILQAVGPKT
AQDWVTRFGFDADKHPAYLTMALGAGSVTPMQMVSAYSVFANGGYRVNPYLITRVTDHKGRILSNFEPPATTDNPRAIDA
RNAFIMDSLLQEVTRSGTAARAQATLKRPDVYGKTGTTNDAMDAWFAGFQPTIAAVTWIGYDTPRNLGSRETGGGLSLPV
WISFMERALKGVPVAEPTVPPGVVNVGGEWFYEEFARNAGVASVGMEDHPAAAVPGAPPQAPPPAEERNRILDLFRN
>Mature_796_residues
PPPPTDKTARESASARPTWLRWLLRSLAWLFAIAAAGALGLALLIAVALAVAYPNLPDISDLADYRPKLPLRVYSAEGAL
LGEFGEERRNLTPIQDIPKVMTNAVLAIEDARFFQHGGVDYKGMVRAALANLGRVKSQGASTITMQVARNVYLSSEKTIT
RKIYEILLTFKLEHLLTKNQILEIYMNQIYLGNRAYGFAAASEAYFGKPLKSISIAEAAMLAGLPKAPSAYNPISNPKRA
RVRQLYIIDRMVENGFITPEEGAAAKQEDLKIRSGGPDNTRIHAEYVAEMARQLIFAQYGSEAYTRGLNVYTTLTAGDQE
AAYQALRKGIMDYERRQHYRGPEKFVALPTDPQEAEDVIDDTLAGHPDNGDVLSAVVLEASPKKIVAARANGEPIEITGD
GLKPAQSGLSDKAPPNIRIRRGAVIRIIQTPKKTWDITQLPEVEGAFVAVDPRNGAIKALVGGFDFDKNKFNHVTQAWRQ
PGSSFKPFIYSAALEKGFTPATVVNDAPLFFSAGTTGGQPWEPKNYDGKYDGPMTLRTALAKSKNVVSIRILQAVGPKTA
QDWVTRFGFDADKHPAYLTMALGAGSVTPMQMVSAYSVFANGGYRVNPYLITRVTDHKGRILSNFEPPATTDNPRAIDAR
NAFIMDSLLQEVTRSGTAARAQATLKRPDVYGKTGTTNDAMDAWFAGFQPTIAAVTWIGYDTPRNLGSRETGGGLSLPVW
ISFMERALKGVPVAEPTVPPGVVNVGGEWFYEEFARNAGVASVGMEDHPAAAVPGAPPQAPPPAEERNRILDLFRN

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG5009

COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=584, Percent_Identity=40.9246575342466, Blast_Score=460, Evalue=1e-130,
Organism=Escherichia coli, GI1786343, Length=269, Percent_Identity=32.7137546468402, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1788867, Length=181, Percent_Identity=37.5690607734807, Blast_Score=106, Evalue=5e-24,
Organism=Escherichia coli, GI1789601, Length=168, Percent_Identity=33.3333333333333, Blast_Score=88, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.-

Molecular weight: Translated: 86807; Mature: 86676

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPPPTDKTARESASARPTWLRWLLRSLAWLFAIAAAGALGLALLIAVALAVAYPNLPDI
CCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
SDLADYRPKLPLRVYSAEGALLGEFGEERRNLTPIQDIPKVMTNAVLAIEDARFFQHGGV
HHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHEEEEEHHHHHHCCCC
DYKGMVRAALANLGRVKSQGASTITMQVARNVYLSSEKTITRKIYEILLTFKLEHLLTKN
CHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
QILEIYMNQIYLGNRAYGFAAASEAYFGKPLKSISIAEAAMLAGLPKAPSAYNPISNPKR
HHHHHHHHHHEECCCCCCCHHCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
ARVRQLYIIDRMVENGFITPEEGAAAKQEDLKIRSGGPDNTRIHAEYVAEMARQLIFAQY
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCEEECCCCCCCEEEHHHHHHHHHHHHHHHH
GSEAYTRGLNVYTTLTAGDQEAAYQALRKGIMDYERRQHYRGPEKFVALPTDPQEAEDVI
CCHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHEEECCCCHHHHHHHH
DDTLAGHPDNGDVLSAVVLEASPKKIVAARANGEPIEITGDGLKPAQSGLSDKAPPNIRI
HHHHCCCCCCCHHHHHEEECCCCCEEEEEECCCCEEEEECCCCCHHHCCCCCCCCCCEEE
RRGAVIRIIQTPKKTWDITQLPEVEGAFVAVDPRNGAIKALVGGFDFDKNKFNHVTQAWR
ECCCEEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHH
QPGSSFKPFIYSAALEKGFTPATVVNDAPLFFSAGTTGGQPWEPKNYDGKYDGPMTLRTA
CCCCCCCHHHHHHHHHCCCCCCEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHH
LAKSKNVVSIRILQAVGPKTAQDWVTRFGFDADKHPAYLTMALGAGSVTPMQMVSAYSVF
HHCCCCEEEEEEEECCCCCCHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHH
ANGGYRVNPYLITRVTDHKGRILSNFEPPATTDNPRAIDARNAFIMDSLLQEVTRSGTAA
CCCCEEECCEEEEEEECCCCCEECCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHCCCHH
RAQATLKRPDVYGKTGTTNDAMDAWFAGFQPTIAAVTWIGYDTPRNLGSRETGGGLSLPV
HHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCHHHEEEEECCCCCCCCCCCCCCCCCCHHH
WISFMERALKGVPVAEPTVPPGVVNVGGEWFYEEFARNAGVASVGMEDHPAAAVPGAPPQ
HHHHHHHHHCCCCCCCCCCCCCEEECCHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCC
APPPAEERNRILDLFRN
CCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
PPPPTDKTARESASARPTWLRWLLRSLAWLFAIAAAGALGLALLIAVALAVAYPNLPDI
CCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
SDLADYRPKLPLRVYSAEGALLGEFGEERRNLTPIQDIPKVMTNAVLAIEDARFFQHGGV
HHHHHCCCCCCEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHEEEEEHHHHHHCCCC
DYKGMVRAALANLGRVKSQGASTITMQVARNVYLSSEKTITRKIYEILLTFKLEHLLTKN
CHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
QILEIYMNQIYLGNRAYGFAAASEAYFGKPLKSISIAEAAMLAGLPKAPSAYNPISNPKR
HHHHHHHHHHEECCCCCCCHHCCHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
ARVRQLYIIDRMVENGFITPEEGAAAKQEDLKIRSGGPDNTRIHAEYVAEMARQLIFAQY
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCEEECCCCCCCEEEHHHHHHHHHHHHHHHH
GSEAYTRGLNVYTTLTAGDQEAAYQALRKGIMDYERRQHYRGPEKFVALPTDPQEAEDVI
CCHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCHHEEECCCCHHHHHHHH
DDTLAGHPDNGDVLSAVVLEASPKKIVAARANGEPIEITGDGLKPAQSGLSDKAPPNIRI
HHHHCCCCCCCHHHHHEEECCCCCEEEEEECCCCEEEEECCCCCHHHCCCCCCCCCCEEE
RRGAVIRIIQTPKKTWDITQLPEVEGAFVAVDPRNGAIKALVGGFDFDKNKFNHVTQAWR
ECCCEEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHH
QPGSSFKPFIYSAALEKGFTPATVVNDAPLFFSAGTTGGQPWEPKNYDGKYDGPMTLRTA
CCCCCCCHHHHHHHHHCCCCCCEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCHHHHHH
LAKSKNVVSIRILQAVGPKTAQDWVTRFGFDADKHPAYLTMALGAGSVTPMQMVSAYSVF
HHCCCCEEEEEEEECCCCCCHHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHH
ANGGYRVNPYLITRVTDHKGRILSNFEPPATTDNPRAIDARNAFIMDSLLQEVTRSGTAA
CCCCEEECCEEEEEEECCCCCEECCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHCCCHH
RAQATLKRPDVYGKTGTTNDAMDAWFAGFQPTIAAVTWIGYDTPRNLGSRETGGGLSLPV
HHHHHHCCCCCCCCCCCCCHHHHHHHCCCCCHHHEEEEECCCCCCCCCCCCCCCCCCHHH
WISFMERALKGVPVAEPTVPPGVVNVGGEWFYEEFARNAGVASVGMEDHPAAAVPGAPPQ
HHHHHHHHHCCCCCCCCCCCCCEEECCHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCC
APPPAEERNRILDLFRN
CCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA