Click here to switch to the map view.

The map label for this gene is gltD [H]

Identifier: 120609660

GI number: 120609660

Start: 1059297

End: 1060652

Strand: Direct

Name: gltD [H]

Synonym: Aave_0966

Alternate gene names: 120609660

Gene position: 1059297-1060652 (Clockwise)

Preceding gene: 120609657

Following gene: 120609661

Centisome position: 19.79

GC content: 72.42

Gene sequence:

>1356_bases
ATGGAAACGACGACCGCGAGCGTTGGCTGCGGCGTGCATGGCGCACGCCTGGACCTGGCCGCCTACGCACACAACTTCAG
CGACGCGCACCCTCCGCTCACCCGCCCGCAGGCGCTGATCGAGGCCGAGCGCTGCTACTACTGTTACGAGGCGCCCTGCG
CCACGGCCTGCCCGACGGGCATCGACGTGCCGTCCTTCATCCACCGCATCGCGCAGGGCAACGAGCGTGGCGCGGCGCGC
GCGATCCTGGAGGCCAACCCGCTGGGCGGCATGTGCGCCCGGGTCTGCCCGACGGAAGTGCTGTGCGAGCAGGCCTGCGT
GCGCAACGTGAACGAGGACAAGCCGGTGGAGATCGGCGCACTGCAGCGGTATGCGACGGACGCGCATTTCGCGCAGCCGG
GCGCGCCGCTCTTCACGCGCTCCGCGTCCACGGGCCGGCGCGTGGCCGTGGTGGGTGCCGGCCCCGCGGGCCTGGCCTGC
GCGCACGGACTGGCGCGGCGCGGGCACGACGTGATGCTGTTCGACGCGCGGCCCAAGCTCGGCGGGCTCAACGAATACGG
CCTCGCGAGCTACAAGACCACGGGCGGCTTCGCGCAGAAGGAAATCGAGTGGCTGCTGTCCATCGGCGGCATCGAACCGC
GCTGCGGGCAGGTGCTGGGGCGCGACATCACGCTCGCGGGGCTGCTGCAGGCTTATGACGCGGTGTTCCTCGGCCTCGGG
CTGGCGGGCGTGAACGCGCTGGGCATCGCCGAGCCGCAGGCGGAAGGACTGCGCAATGCGGTGGACTTCATCGCCGACCT
GCGGCAGGCGCAGGACCTCTCCACGCTGCCCGTGGGCTGCCGCGTGGTGGTGGTGGGCGGCGGCATGACGGCGGTGGATG
CCGCCGTGCAGGCCGCCAAGCTCGGCGCGGAGGAGGTGACCATGGTCTATCGCCGCGGGCAGGAGAGCATGTCCGCCTCG
CTCGTGGAGCAGCAGTGGGCGCAGACGAATGGCGTCACGGTGCGGTACTGGGCCGCGCCGAAGGAACTGCTCTGCGAAGG
CGGGGCGGTGCGCGGCGTGCGATTCGCGGCCACGGCGGTGCGGGGCGGGCGCCTGGTGGAAACCGGCGAGACCTTCGCGC
TCGAGGCGGACATGGTGCTCAAGGCGATCGGCCAGAGCTATCTCTCCGAATACGCCGGCGCCGAGGTGGCGCTGAGTCAG
GGCCGCATCGCCACCGATGCCGACGGCCGCACCAGCGTGCCGCGCGTCTGGGCGGGCGGCGACTGCCGTGCCGGCGGGCG
CGACCTCACCGTGGAAGCCGTGGAGCACGGCAAGGTGGCCGCGGTGTCCATCCACGAAGCGCTGCTGGCGGCCTGA

Upstream 100 bases:

>100_bases
GCCGCCGGGCTGGCCGCTTCCACCGCACGCCCCAGGCGCGCGCTTCCTCCCCCCTGCCTTCCTGATCCCCGAGCGGGACA
TACGACACAGGAGCCTTCCG

Downstream 100 bases:

>100_bases
AAGCCGCCGCCGACCCCACGACCCCCGGAGCAAGACAACGGAGAAACACATGGCAGACATCCGCAGCGATTTCCTCGGCA
TCCGCAGCCCGAACCCCTTC

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
METTTASVGCGVHGARLDLAAYAHNFSDAHPPLTRPQALIEAERCYYCYEAPCATACPTGIDVPSFIHRIAQGNERGAAR
AILEANPLGGMCARVCPTEVLCEQACVRNVNEDKPVEIGALQRYATDAHFAQPGAPLFTRSASTGRRVAVVGAGPAGLAC
AHGLARRGHDVMLFDARPKLGGLNEYGLASYKTTGGFAQKEIEWLLSIGGIEPRCGQVLGRDITLAGLLQAYDAVFLGLG
LAGVNALGIAEPQAEGLRNAVDFIADLRQAQDLSTLPVGCRVVVVGGGMTAVDAAVQAAKLGAEEVTMVYRRGQESMSAS
LVEQQWAQTNGVTVRYWAAPKELLCEGGAVRGVRFAATAVRGGRLVETGETFALEADMVLKAIGQSYLSEYAGAEVALSQ
GRIATDADGRTSVPRVWAGGDCRAGGRDLTVEAVEHGKVAAVSIHEALLAA

Sequences:

>Translated_451_residues
METTTASVGCGVHGARLDLAAYAHNFSDAHPPLTRPQALIEAERCYYCYEAPCATACPTGIDVPSFIHRIAQGNERGAAR
AILEANPLGGMCARVCPTEVLCEQACVRNVNEDKPVEIGALQRYATDAHFAQPGAPLFTRSASTGRRVAVVGAGPAGLAC
AHGLARRGHDVMLFDARPKLGGLNEYGLASYKTTGGFAQKEIEWLLSIGGIEPRCGQVLGRDITLAGLLQAYDAVFLGLG
LAGVNALGIAEPQAEGLRNAVDFIADLRQAQDLSTLPVGCRVVVVGGGMTAVDAAVQAAKLGAEEVTMVYRRGQESMSAS
LVEQQWAQTNGVTVRYWAAPKELLCEGGAVRGVRFAATAVRGGRLVETGETFALEADMVLKAIGQSYLSEYAGAEVALSQ
GRIATDADGRTSVPRVWAGGDCRAGGRDLTVEAVEHGKVAAVSIHEALLAA
>Mature_451_residues
METTTASVGCGVHGARLDLAAYAHNFSDAHPPLTRPQALIEAERCYYCYEAPCATACPTGIDVPSFIHRIAQGNERGAAR
AILEANPLGGMCARVCPTEVLCEQACVRNVNEDKPVEIGALQRYATDAHFAQPGAPLFTRSASTGRRVAVVGAGPAGLAC
AHGLARRGHDVMLFDARPKLGGLNEYGLASYKTTGGFAQKEIEWLLSIGGIEPRCGQVLGRDITLAGLLQAYDAVFLGLG
LAGVNALGIAEPQAEGLRNAVDFIADLRQAQDLSTLPVGCRVVVVGGGMTAVDAAVQAAKLGAEEVTMVYRRGQESMSAS
LVEQQWAQTNGVTVRYWAAPKELLCEGGAVRGVRFAATAVRGGRLVETGETFALEADMVLKAIGQSYLSEYAGAEVALSQ
GRIATDADGRTSVPRVWAGGDCRAGGRDLTVEAVEHGKVAAVSIHEALLAA

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=453, Percent_Identity=33.7748344370861, Blast_Score=194, Evalue=2e-49,
Organism=Homo sapiens, GI237757300, Length=106, Percent_Identity=46.2264150943396, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1789606, Length=446, Percent_Identity=34.7533632286996, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1788811, Length=459, Percent_Identity=34.2047930283224, Blast_Score=205, Evalue=4e-54,
Organism=Escherichia coli, GI87082180, Length=442, Percent_Identity=34.3891402714932, Blast_Score=188, Evalue=6e-49,
Organism=Escherichia coli, GI1788468, Length=426, Percent_Identity=34.037558685446, Blast_Score=187, Evalue=9e-49,
Organism=Escherichia coli, GI1789243, Length=387, Percent_Identity=29.9741602067183, Blast_Score=115, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI71984108, Length=472, Percent_Identity=33.2627118644068, Blast_Score=185, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI17570289, Length=458, Percent_Identity=24.4541484716157, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6320030, Length=490, Percent_Identity=28.1632653061224, Blast_Score=130, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24640763, Length=449, Percent_Identity=34.5211581291759, Blast_Score=200, Evalue=2e-51,
Organism=Drosophila melanogaster, GI18858217, Length=449, Percent_Identity=34.5211581291759, Blast_Score=200, Evalue=2e-51,
Organism=Drosophila melanogaster, GI24665547, Length=462, Percent_Identity=28.5714285714286, Blast_Score=152, Evalue=6e-37,
Organism=Drosophila melanogaster, GI24665543, Length=462, Percent_Identity=28.5714285714286, Blast_Score=152, Evalue=6e-37,
Organism=Drosophila melanogaster, GI28574881, Length=462, Percent_Identity=28.5714285714286, Blast_Score=151, Evalue=7e-37,
Organism=Drosophila melanogaster, GI24665539, Length=462, Percent_Identity=28.5714285714286, Blast_Score=151, Evalue=7e-37,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 47174; Mature: 47174

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METTTASVGCGVHGARLDLAAYAHNFSDAHPPLTRPQALIEAERCYYCYEAPCATACPTG
CCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHEEEEECCCCCCCCCCC
IDVPSFIHRIAQGNERGAARAILEANPLGGMCARVCPTEVLCEQACVRNVNEDKPVEIGA
CCHHHHHHHHHCCCCCCHHEEEEECCCCCCHHHHHCCHHHHHHHHHHHCCCCCCCEEHHH
LQRYATDAHFAQPGAPLFTRSASTGRRVAVVGAGPAGLACAHGLARRGHDVMLFDARPKL
HHHHHCCCCCCCCCCCEEECCCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCC
GGLNEYGLASYKTTGGFAQKEIEWLLSIGGIEPRCGQVLGRDITLAGLLQAYDAVFLGLG
CCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHH
LAGVNALGIAEPQAEGLRNAVDFIADLRQAQDLSTLPVGCRVVVVGGGMTAVDAAVQAAK
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHH
LGAEEVTMVYRRGQESMSASLVEQQWAQTNGVTVRYWAAPKELLCEGGAVRGVRFAATAV
CCHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHCCCCCCCEEEEEHHH
RGGRLVETGETFALEADMVLKAIGQSYLSEYAGAEVALSQGRIATDADGRTSVPRVWAGG
CCCEEEECCCEEEEHHHHHHHHHHHHHHHHHCCCEEEECCCCEEECCCCCCCCCEEECCC
DCRAGGRDLTVEAVEHGKVAAVSIHEALLAA
CCCCCCCCEEEEEECCCCEEEEEHHHHHHCC
>Mature Secondary Structure
METTTASVGCGVHGARLDLAAYAHNFSDAHPPLTRPQALIEAERCYYCYEAPCATACPTG
CCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHEEEEECCCCCCCCCCC
IDVPSFIHRIAQGNERGAARAILEANPLGGMCARVCPTEVLCEQACVRNVNEDKPVEIGA
CCHHHHHHHHHCCCCCCHHEEEEECCCCCCHHHHHCCHHHHHHHHHHHCCCCCCCEEHHH
LQRYATDAHFAQPGAPLFTRSASTGRRVAVVGAGPAGLACAHGLARRGHDVMLFDARPKL
HHHHHCCCCCCCCCCCEEECCCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEECCCCC
GGLNEYGLASYKTTGGFAQKEIEWLLSIGGIEPRCGQVLGRDITLAGLLQAYDAVFLGLG
CCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHH
LAGVNALGIAEPQAEGLRNAVDFIADLRQAQDLSTLPVGCRVVVVGGGMTAVDAAVQAAK
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHH
LGAEEVTMVYRRGQESMSASLVEQQWAQTNGVTVRYWAAPKELLCEGGAVRGVRFAATAV
CCHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHCCCCCCCEEEEEHHH
RGGRLVETGETFALEADMVLKAIGQSYLSEYAGAEVALSQGRIATDADGRTSVPRVWAGG
CCCEEEECCCEEEEHHHHHHHHHHHHHHHHHCCCEEEECCCCEEECCCCCCCCCEEECCC
DCRAGGRDLTVEAVEHGKVAAVSIHEALLAA
CCCCCCCCEEEEEECCCCEEEEEHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]