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The map label for this gene is wecB [C]

Identifier: 120609647

GI number: 120609647

Start: 1038975

End: 1040057

Strand: Direct

Name: wecB [C]

Synonym: Aave_0953

Alternate gene names: 120609647

Gene position: 1038975-1040057 (Clockwise)

Preceding gene: 120609646

Following gene: 120609648

Centisome position: 19.41

GC content: 55.4

Gene sequence:

>1083_bases
ATGAAAAAAATTCTTACTGTAGTGGGTGCCCGCCCCCAATTCATCAAGGCGGCAGCCATATCGCGGGCCATCTCCCAGCG
CCATGTCGATCGTTTTATAGAAGTCCTAGTCCATACAGGGCAACACTACGATGCGAACATGTCGCAGGTGTTTTTTGATG
AACTGCAGATTCCGGCTCCTAAATACAACATGGAAGTGTCAGGTGGGGGACATGGCGTCATGACCGGACGCATGTTGGAA
GGAGTCGAGAAGATCATTCAGGAAGAGCGTCCGGATTGGCTGCTCATCTATGGAGATACCAACTCCACTCTCGCTGGAGC
ACTCGCAGCCGCCAAATTGCACATTCCCATCGCGCACGTAGAGGCCGGGCTCAGGTCTTTCAACATGCGGATGCCGGAGG
AAATCAACCGCATCCTCTCGGACAGGGTTTCCAGCCTGCTTTTCTGTCCTACTGCAGCAGCAGTGGAGAACCTGAGAAGA
GAAGGGCTGGAAAAAGGAGTGCATAATGTCGGCGATGTGACGTATGACGTCGCCCTGTTCTACAGGGAGATTGCGCTGCA
GCGCAGCGAAATATTGAATCGCCTTGGCGTCAAGTCTGGCGATTACGTTCTCGCCACTTGCCATCGTGCGGAAAATACGG
ACGATCCAGGGCGGATGTCTCAGATATTGGAGGCCCTGGGCAGGATCGTGGAAACTCGCCCGGTCGTTTTGCCCCTGCAC
CCGAGAACCAGAAATGCCATCCATGGCTATGGACTGGGCGACCGTCTGAACAAAATCATGGTCACAGATCCGTTGCCATA
CCTCGACATCGTCGCGCTGGAGCAATGTGCGCACACCATCGTCACCGACTCGGGCGGCGTACAGAAAGAGGCTTTCTTCT
ACGGTGTTCCTTGCGTCACCGTGCGGAGCGAAACGGAGTGGATCGAAACCGTCGATTCCGGGTGGAATACGATCGCCGGT
GCTGATGCCCGCAAGATACTGGACGCCATGGATGCAGGCCGCCCTGCTGCCTCGAAACCTGAAGTTTATGGCAAGGGTGA
CGCGGCAGACCGTATTCTGGACATCATGGGCGCTGATCGATGA

Upstream 100 bases:

>100_bases
GTGAGGCGGCGAATCGCATCATCAGTCTGCCCATGAGTGCCGACATTACTGAGCAAGACCAGCGAGACGTCGTTGCTGCA
CTGGCAATGGCATTGAAAAA

Downstream 100 bases:

>100_bases
ATCTGATCCATCTCATCTGGGCGCACCGCTGGATCATCGCTGCGACCACCGTGGTCGATGTGAAGTCGAGATTCCGGGGA
ACGGTGATAGGAATCCTCTG

Product: UDP-N-acetylglucosamine 2-epimerase

Products: UDP-N-acetyl-D-mannosamine

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKKILTVVGARPQFIKAAAISRAISQRHVDRFIEVLVHTGQHYDANMSQVFFDELQIPAPKYNMEVSGGGHGVMTGRMLE
GVEKIIQEERPDWLLIYGDTNSTLAGALAAAKLHIPIAHVEAGLRSFNMRMPEEINRILSDRVSSLLFCPTAAAVENLRR
EGLEKGVHNVGDVTYDVALFYREIALQRSEILNRLGVKSGDYVLATCHRAENTDDPGRMSQILEALGRIVETRPVVLPLH
PRTRNAIHGYGLGDRLNKIMVTDPLPYLDIVALEQCAHTIVTDSGGVQKEAFFYGVPCVTVRSETEWIETVDSGWNTIAG
ADARKILDAMDAGRPAASKPEVYGKGDAADRILDIMGADR

Sequences:

>Translated_360_residues
MKKILTVVGARPQFIKAAAISRAISQRHVDRFIEVLVHTGQHYDANMSQVFFDELQIPAPKYNMEVSGGGHGVMTGRMLE
GVEKIIQEERPDWLLIYGDTNSTLAGALAAAKLHIPIAHVEAGLRSFNMRMPEEINRILSDRVSSLLFCPTAAAVENLRR
EGLEKGVHNVGDVTYDVALFYREIALQRSEILNRLGVKSGDYVLATCHRAENTDDPGRMSQILEALGRIVETRPVVLPLH
PRTRNAIHGYGLGDRLNKIMVTDPLPYLDIVALEQCAHTIVTDSGGVQKEAFFYGVPCVTVRSETEWIETVDSGWNTIAG
ADARKILDAMDAGRPAASKPEVYGKGDAADRILDIMGADR
>Mature_360_residues
MKKILTVVGARPQFIKAAAISRAISQRHVDRFIEVLVHTGQHYDANMSQVFFDELQIPAPKYNMEVSGGGHGVMTGRMLE
GVEKIIQEERPDWLLIYGDTNSTLAGALAAAKLHIPIAHVEAGLRSFNMRMPEEINRILSDRVSSLLFCPTAAAVENLRR
EGLEKGVHNVGDVTYDVALFYREIALQRSEILNRLGVKSGDYVLATCHRAENTDDPGRMSQILEALGRIVETRPVVLPLH
PRTRNAIHGYGLGDRLNKIMVTDPLPYLDIVALEQCAHTIVTDSGGVQKEAFFYGVPCVTVRSETEWIETVDSGWNTIAG
ADARKILDAMDAGRPAASKPEVYGKGDAADRILDIMGADR

Specific function: Catalyzes The Reversible Epimerization At C-2 Of UDP-N- Acetylglucosamine (UDP-Glcnac) And Thereby Provides Bacteria With UDP-N-Acetylmannosamine (UDP-Mannac), The Activated Donor Of Mannac Residues. Also Involved In Bacteriophage N4 Adsorption. [C]

COG id: COG0381

COG function: function code M; UDP-N-acetylglucosamine 2-epimerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-N-acetylglucosamine 2-epimerase family [H]

Homologues:

Organism=Escherichia coli, GI48994967, Length=382, Percent_Identity=32.4607329842932, Blast_Score=145, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003331 [H]

Pfam domain/function: PF02350 Epimerase_2 [H]

EC number: 5.1.3.14

Molecular weight: Translated: 39639; Mature: 39639

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILTVVGARPQFIKAAAISRAISQRHVDRFIEVLVHTGQHYDANMSQVFFDELQIPAP
CCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCC
KYNMEVSGGGHGVMTGRMLEGVEKIIQEERPDWLLIYGDTNSTLAGALAAAKLHIPIAHV
CCCEEECCCCCCEEHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCHHHHH
EAGLRSFNMRMPEEINRILSDRVSSLLFCPTAAAVENLRREGLEKGVHNVGDVTYDVALF
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
YREIALQRSEILNRLGVKSGDYVLATCHRAENTDDPGRMSQILEALGRIVETRPVVLPLH
HHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEECC
PRTRNAIHGYGLGDRLNKIMVTDPLPYLDIVALEQCAHTIVTDSGGVQKEAFFYGVPCVT
CCCCCCCCCCCCHHHHCEEEEECCCCHHHHHHHHHHHHHHEECCCCCCCCCEEECCCEEE
VRSETEWIETVDSGWNTIAGADARKILDAMDAGRPAASKPEVYGKGDAADRILDIMGADR
ECCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MKKILTVVGARPQFIKAAAISRAISQRHVDRFIEVLVHTGQHYDANMSQVFFDELQIPAP
CCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCC
KYNMEVSGGGHGVMTGRMLEGVEKIIQEERPDWLLIYGDTNSTLAGALAAAKLHIPIAHV
CCCEEECCCCCCEEHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCHHHHH
EAGLRSFNMRMPEEINRILSDRVSSLLFCPTAAAVENLRREGLEKGVHNVGDVTYDVALF
HHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
YREIALQRSEILNRLGVKSGDYVLATCHRAENTDDPGRMSQILEALGRIVETRPVVLPLH
HHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEECC
PRTRNAIHGYGLGDRLNKIMVTDPLPYLDIVALEQCAHTIVTDSGGVQKEAFFYGVPCVT
CCCCCCCCCCCCHHHHCEEEEECCCCHHHHHHHHHHHHHHEECCCCCCCCCEEECCCEEE
VRSETEWIETVDSGWNTIAGADARKILDAMDAGRPAASKPEVYGKGDAADRILDIMGADR
ECCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: UDP-N-acetylglucosamine [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.63 {UDP-N-acetylglucosamine}} 1 {UDP-N-acetylmannosamine}} 0.5 {UDP-N-acetylmannosamine}} [C]

Substrates: UDP-N-acetyl-D-glucosamine

Specific reaction: UDP-N-acetyl-D-glucosamine = UDP-N-acetyl-D-mannosamine

General reaction: Epimerization [C]

Inhibitor: PCMB [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA