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The map label for this gene is rhtB [H]

Identifier: 120609284

GI number: 120609284

Start: 637048

End: 637731

Strand: Direct

Name: rhtB [H]

Synonym: Aave_0584

Alternate gene names: 120609284

Gene position: 637048-637731 (Clockwise)

Preceding gene: 120609283

Following gene: 120609285

Centisome position: 11.9

GC content: 73.98

Gene sequence:

>684_bases
GTGCCGGATTTCCACCATCTGCTGCTGTTCATCGCCGCGGGCTGGCTGCTGAACCTCACGCCCGGGCCGGATGTCTTCTA
CATCGCCACGCATGCCCTGCGATCGGGGGCGCGCGCCGGCATCGTGGCGGGCCTGGGCGTCACCGCGGGCTGCTTCGTCC
ACGTGGCCGCCGCGGCGCTCGGCGTGGGGGCGCTGCTTGCCACGTCGGCCACCGCGTTCTCCGTGCTCAAGTGGGCCGGT
GCGGCCTACCTGGTCTGGATGGGCGTGCGCATGCTGCGCGCCCGGCCGGGCGGCGGCCTCGCGGCCGTGGCCCGCACCGC
GGGACCGGCGGGCGAGGGCGCCGCCGCTGTGGACGCCGCAGCCGGCGCGCCGCTGCGCAAGGTCTTCCTGGGCGGCTTCT
GGACCAACGTGCTCAACCCGAAGGTGGCCATCTTCTTCCTGGCCTTCGTTCCCCAGTTCATCGCGCCGGGCGCGGAGCAC
GAGGCCCTGGCCTTCGTGCTGCTGGGCACGCTGTTCAACCTCAATGCCATCCCCGTGAACGTGGGCTGGGCCCTGGCCGC
CGCCTGGATTGCGCGGCGCGCCGCCCTGGTGCAGCGCCGCATGCACTGGCTCGACCGCGCGGCCGGCGCCATGTTCGTCG
GCTTCGGCATCAGGCTCGCGCTGTCCGAGCGGCCGGGCCCCTGA

Upstream 100 bases:

>100_bases
TGAAGAACTTCCTCTCGATGAAGGCGTGACCGCCATGGACGCCTCCCCGGTCTCCACCGGTCCCGCGCCGGCCGCCGCGG
GTCGCCGGTAAGGATTCGCC

Downstream 100 bases:

>100_bases
ATCTCCCGATCTCCAGAAAAAGGCTTCCCGCCATGTCCATCACCCCCCAGGAAGCGCTGCAGCGCACGATCGAGCACCGC
GAAATCTTCCACGACGAGAT

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MPDFHHLLLFIAAGWLLNLTPGPDVFYIATHALRSGARAGIVAGLGVTAGCFVHVAAAALGVGALLATSATAFSVLKWAG
AAYLVWMGVRMLRARPGGGLAAVARTAGPAGEGAAAVDAAAGAPLRKVFLGGFWTNVLNPKVAIFFLAFVPQFIAPGAEH
EALAFVLLGTLFNLNAIPVNVGWALAAAWIARRAALVQRRMHWLDRAAGAMFVGFGIRLALSERPGP

Sequences:

>Translated_227_residues
MPDFHHLLLFIAAGWLLNLTPGPDVFYIATHALRSGARAGIVAGLGVTAGCFVHVAAAALGVGALLATSATAFSVLKWAG
AAYLVWMGVRMLRARPGGGLAAVARTAGPAGEGAAAVDAAAGAPLRKVFLGGFWTNVLNPKVAIFFLAFVPQFIAPGAEH
EALAFVLLGTLFNLNAIPVNVGWALAAAWIARRAALVQRRMHWLDRAAGAMFVGFGIRLALSERPGP
>Mature_226_residues
PDFHHLLLFIAAGWLLNLTPGPDVFYIATHALRSGARAGIVAGLGVTAGCFVHVAAAALGVGALLATSATAFSVLKWAGA
AYLVWMGVRMLRARPGGGLAAVARTAGPAGEGAAAVDAAAGAPLRKVFLGGFWTNVLNPKVAIFFLAFVPQFIAPGAEHE
ALAFVLLGTLFNLNAIPVNVGWALAAAWIARRAALVQRRMHWLDRAAGAMFVGFGIRLALSERPGP

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=214, Percent_Identity=29.9065420560748, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI48994978, Length=209, Percent_Identity=31.1004784688995, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1788099, Length=202, Percent_Identity=25.7425742574257, Blast_Score=75, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 23513; Mature: 23382

Theoretical pI: Translated: 11.80; Mature: 11.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDFHHLLLFIAAGWLLNLTPGPDVFYIATHALRSGARAGIVAGLGVTAGCFVHVAAAAL
CCHHHHHHHHHHHHHEEECCCCCCEEHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHH
GVGALLATSATAFSVLKWAGAAYLVWMGVRMLRARPGGGLAAVARTAGPAGEGAAAVDAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCHHHHHH
AGAPLRKVFLGGFWTNVLNPKVAIFFLAFVPQFIAPGAEHEALAFVLLGTLFNLNAIPVN
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEC
VGWALAAAWIARRAALVQRRMHWLDRAAGAMFVGFGIRLALSERPGP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHCEEEEECCCCCC
>Mature Secondary Structure 
PDFHHLLLFIAAGWLLNLTPGPDVFYIATHALRSGARAGIVAGLGVTAGCFVHVAAAAL
CHHHHHHHHHHHHHEEECCCCCCEEHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHH
GVGALLATSATAFSVLKWAGAAYLVWMGVRMLRARPGGGLAAVARTAGPAGEGAAAVDAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCHHHHHH
AGAPLRKVFLGGFWTNVLNPKVAIFFLAFVPQFIAPGAEHEALAFVLLGTLFNLNAIPVN
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEC
VGWALAAAWIARRAALVQRRMHWLDRAAGAMFVGFGIRLALSERPGP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]