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The map label for this gene is phbI [H]

Identifier: 120609057

GI number: 120609057

Start: 378751

End: 380529

Strand: Direct

Name: phbI [H]

Synonym: Aave_0354

Alternate gene names: 120609057

Gene position: 378751-380529 (Clockwise)

Preceding gene: 120609056

Following gene: 120609058

Centisome position: 7.08

GC content: 70.43

Gene sequence:

>1779_bases
ATGACATTCGCCGTCCACGGCCTGGCCGTCGCCCGGGGCATCGCCATCGGGCGCGCGGTGCTCGTGGCGTCGAGCCGGGT
GGACGTGGCGCACTATTTCATCGAGCCCGAGCAGGTGCGCGCGGAGATCATCCGCGTGCGCAACGGGCGCAATGCCGTGG
TCGAGGAGCTGCAGCGCCTGCAGGCCGAGCTGCCGGCCGATGCTCCGCCCGAGCTCGCCGCGCTGCTGGACGTGCACCTG
ATGCTGCTGCAGGACGAGGCGCTGGTGAGCGGCGTCAAGCACTGGATCACGGACCGCCTCTACAACGCCGAATGGGCGCT
GACCACGCAACTGGAGATCATCGCGCGCCAGTTCGACGAGATGGAGGACGAATACCTGCGCGAGCGCAAGGCCGACCTGG
AACAGGTGGTCGAGCGCATCCTGCGGCACATGAAGGGGGTGGCAAGCCCCGTCGCGCCGCCGCCCTGCAGTCCGCGCCGC
GGCAGCGCCGCGCTGGGCTTGCAACAGGACCTGCTGCCGTGCGACATGGTGGACGTGCCGCTGGTGCTGGTGGCGCACGA
TCTCTCGCCGGCCGACATGCTGCAGTTCAAGCAGAGCGTGTTCGCGGGCTTCGTGACCGACGTGGGCGGCAAGACCTCGC
ACACGGCCATCGTGGCGCGCAGCATGGACATTCCCGCCGTGGTGGGCGCGCGTGCCGCGAGCCAGCTGGTGCGGCAGGAC
GACTGGGTGATCATCGACGGGGATGCGGGCATCATGATCGTCGATCCGTCGCCGATCATCCTGGCCGAGTACGGCTTCCG
CCAGCGGCAGACCGAACTGGAGCGCGAACGGCTGTCGCGCCTGCGGCACACGCCGGCGGTGACGCTCGACGGCGAGCGCA
TCGAACTGCTGGCGAACATCGAGCAGCCGGCCGATGCCGCGGCCGCCGTGCGTGCCGGCGCGGCGGGCGTGGGGCTGTTC
CGCAGCGAATTCATGTTCATGGGCCGGGGCGGCCACCTGCCGGGCGAGGAGGAGCAGTACCAGGCCTACCGCGCCGCGGT
GGAGGGCATGCAGGGCCTGCCCGTCACGCTGCGCACCATCGACGTGGGCGCGGACAAGCCGCTGGACCACAAGGCCGCCA
AGGAGGCAGCCAGCCACCTCAACCCGGCGCTGGGCCTGCGGGCGATCCGCTGGAGCCTGGCCGATCCGACCATGTTCCGC
ACGCAGTTGCGGGCCGTGCTGCGCGCGGCCGCGCACGGGCCGGTGAACCTGCTGTTCCCGATGCTCGCGCACGTCGGTGA
GATCCGGCAGACACTGGCGCAGGTGGACATGGCACGCGCCGAGCTGGATGCGCGCGGTGCATCCTACGGGCCGGTGCGGC
TGGGGGCGATGATCGAGGTGCCCGCGGCGGCGCTGATCGTGCGGCGCTTCCTGCAGTATTTCGATTTCCTGTCGATCGGC
ACCAACGACCTGATCCAGTACACCCTGGCGATCGACCGTGCCGACGAGAGCGTGGCCCACCTCTACGATCCCATGCACCC
GGCCGTGCTGCAGCTCGTGGCGGACGTGATCGCCGAAGGCCACCGGCAGGGCAAGAACGTGTGCCTTTGCGGCGAGGCCG
CGGGCGACGTGGCGATGACACGGCTGCTGCTGGGCCTGGGCCTGCGCAGCTTCTCCATGCATCCCGCGCAGATCCTGGCG
GTGAAGCAGGAAGTGCTGCGTTCGGACACCCGCAAGCTGGCGCCCTGGGCGCGCACGGTGATCGACGCGGACAACCCGGC
GGACGTGCTGGGCGGCTGA

Upstream 100 bases:

>100_bases
GCGCTGCGCGCGCTCATCGACGGCAAGTTCGGCGAGGGGGAGTGAGTCCGGGAATGACCCCCTCAGCAACCCGTTCAGCA
GCCCGTTACGGAGACCCCCG

Downstream 100 bases:

>100_bases
CGGCCGTCCGCGGCCTGCGCCTGGCGTCGGTCCGCCGAAACTCCGGCCCGGCCCGGCCCGGTGCGCCTTGGCCTGCGCGC
GGTTTTTACGCCGCGTTTAT

Product: phosphoenolpyruvate--protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I; Protein I [H]

Number of amino acids: Translated: 592; Mature: 591

Protein sequence:

>592_residues
MTFAVHGLAVARGIAIGRAVLVASSRVDVAHYFIEPEQVRAEIIRVRNGRNAVVEELQRLQAELPADAPPELAALLDVHL
MLLQDEALVSGVKHWITDRLYNAEWALTTQLEIIARQFDEMEDEYLRERKADLEQVVERILRHMKGVASPVAPPPCSPRR
GSAALGLQQDLLPCDMVDVPLVLVAHDLSPADMLQFKQSVFAGFVTDVGGKTSHTAIVARSMDIPAVVGARAASQLVRQD
DWVIIDGDAGIMIVDPSPIILAEYGFRQRQTELERERLSRLRHTPAVTLDGERIELLANIEQPADAAAAVRAGAAGVGLF
RSEFMFMGRGGHLPGEEEQYQAYRAAVEGMQGLPVTLRTIDVGADKPLDHKAAKEAASHLNPALGLRAIRWSLADPTMFR
TQLRAVLRAAAHGPVNLLFPMLAHVGEIRQTLAQVDMARAELDARGASYGPVRLGAMIEVPAAALIVRRFLQYFDFLSIG
TNDLIQYTLAIDRADESVAHLYDPMHPAVLQLVADVIAEGHRQGKNVCLCGEAAGDVAMTRLLLGLGLRSFSMHPAQILA
VKQEVLRSDTRKLAPWARTVIDADNPADVLGG

Sequences:

>Translated_592_residues
MTFAVHGLAVARGIAIGRAVLVASSRVDVAHYFIEPEQVRAEIIRVRNGRNAVVEELQRLQAELPADAPPELAALLDVHL
MLLQDEALVSGVKHWITDRLYNAEWALTTQLEIIARQFDEMEDEYLRERKADLEQVVERILRHMKGVASPVAPPPCSPRR
GSAALGLQQDLLPCDMVDVPLVLVAHDLSPADMLQFKQSVFAGFVTDVGGKTSHTAIVARSMDIPAVVGARAASQLVRQD
DWVIIDGDAGIMIVDPSPIILAEYGFRQRQTELERERLSRLRHTPAVTLDGERIELLANIEQPADAAAAVRAGAAGVGLF
RSEFMFMGRGGHLPGEEEQYQAYRAAVEGMQGLPVTLRTIDVGADKPLDHKAAKEAASHLNPALGLRAIRWSLADPTMFR
TQLRAVLRAAAHGPVNLLFPMLAHVGEIRQTLAQVDMARAELDARGASYGPVRLGAMIEVPAAALIVRRFLQYFDFLSIG
TNDLIQYTLAIDRADESVAHLYDPMHPAVLQLVADVIAEGHRQGKNVCLCGEAAGDVAMTRLLLGLGLRSFSMHPAQILA
VKQEVLRSDTRKLAPWARTVIDADNPADVLGG
>Mature_591_residues
TFAVHGLAVARGIAIGRAVLVASSRVDVAHYFIEPEQVRAEIIRVRNGRNAVVEELQRLQAELPADAPPELAALLDVHLM
LLQDEALVSGVKHWITDRLYNAEWALTTQLEIIARQFDEMEDEYLRERKADLEQVVERILRHMKGVASPVAPPPCSPRRG
SAALGLQQDLLPCDMVDVPLVLVAHDLSPADMLQFKQSVFAGFVTDVGGKTSHTAIVARSMDIPAVVGARAASQLVRQDD
WVIIDGDAGIMIVDPSPIILAEYGFRQRQTELERERLSRLRHTPAVTLDGERIELLANIEQPADAAAAVRAGAAGVGLFR
SEFMFMGRGGHLPGEEEQYQAYRAAVEGMQGLPVTLRTIDVGADKPLDHKAAKEAASHLNPALGLRAIRWSLADPTMFRT
QLRAVLRAAAHGPVNLLFPMLAHVGEIRQTLAQVDMARAELDARGASYGPVRLGAMIEVPAAALIVRRFLQYFDFLSIGT
NDLIQYTLAIDRADESVAHLYDPMHPAVLQLVADVIAEGHRQGKNVCLCGEAAGDVAMTRLLLGLGLRSFSMHPAQILAV
KQEVLRSDTRKLAPWARTVIDADNPADVLGG

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=561, Percent_Identity=37.6114081996435, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI1789193, Length=540, Percent_Identity=34.4444444444444, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI1788726, Length=592, Percent_Identity=29.222972972973, Blast_Score=250, Evalue=2e-67,
Organism=Escherichia coli, GI48994992, Length=536, Percent_Identity=32.6492537313433, Blast_Score=250, Evalue=2e-67,
Organism=Escherichia coli, GI1787994, Length=511, Percent_Identity=25.440313111546, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI226510935, Length=186, Percent_Identity=24.7311827956989, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 64571; Mature: 64440

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFAVHGLAVARGIAIGRAVLVASSRVDVAHYFIEPEQVRAEIIRVRNGRNAVVEELQRL
CCEEEHHHHHHHHHHHHHHHHEECCCCCHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHH
QAELPADAPPELAALLDVHLMLLQDEALVSGVKHWITDRLYNAEWALTTQLEIIARQFDE
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHH
MEDEYLRERKADLEQVVERILRHMKGVASPVAPPPCSPRRGSAALGLQQDLLPCDMVDVP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHCCCCCCHHCCC
LVLVAHDLSPADMLQFKQSVFAGFVTDVGGKTSHTAIVARSMDIPAVVGARAASQLVRQD
CEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCC
DWVIIDGDAGIMIVDPSPIILAEYGFRQRQTELERERLSRLRHTPAVTLDGERIELLANI
CEEEEECCCCEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEECC
EQPADAAAAVRAGAAGVGLFRSEFMFMGRGGHLPGEEEQYQAYRAAVEGMQGLPVTLRTI
CCCHHHHHHHHCCCHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEE
DVGADKPLDHKAAKEAASHLNPALGLRAIRWSLADPTMFRTQLRAVLRAAAHGPVNLLFP
ECCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHH
MLAHVGEIRQTLAQVDMARAELDARGASYGPVRLGAMIEVPAAALIVRRFLQYFDFLSIG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHCC
TNDLIQYTLAIDRADESVAHLYDPMHPAVLQLVADVIAEGHRQGKNVCLCGEAAGDVAMT
CHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHH
RLLLGLGLRSFSMHPAQILAVKQEVLRSDTRKLAPWARTVIDADNPADVLGG
HHHHHHCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCC
>Mature Secondary Structure 
TFAVHGLAVARGIAIGRAVLVASSRVDVAHYFIEPEQVRAEIIRVRNGRNAVVEELQRL
CEEEHHHHHHHHHHHHHHHHEECCCCCHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHH
QAELPADAPPELAALLDVHLMLLQDEALVSGVKHWITDRLYNAEWALTTQLEIIARQFDE
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHH
MEDEYLRERKADLEQVVERILRHMKGVASPVAPPPCSPRRGSAALGLQQDLLPCDMVDVP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHCCCCCCHHCCC
LVLVAHDLSPADMLQFKQSVFAGFVTDVGGKTSHTAIVARSMDIPAVVGARAASQLVRQD
CEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCC
DWVIIDGDAGIMIVDPSPIILAEYGFRQRQTELERERLSRLRHTPAVTLDGERIELLANI
CEEEEECCCCEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEECC
EQPADAAAAVRAGAAGVGLFRSEFMFMGRGGHLPGEEEQYQAYRAAVEGMQGLPVTLRTI
CCCHHHHHHHHCCCHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEE
DVGADKPLDHKAAKEAASHLNPALGLRAIRWSLADPTMFRTQLRAVLRAAAHGPVNLLFP
ECCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHH
MLAHVGEIRQTLAQVDMARAELDARGASYGPVRLGAMIEVPAAALIVRRFLQYFDFLSIG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHCC
TNDLIQYTLAIDRADESVAHLYDPMHPAVLQLVADVIAEGHRQGKNVCLCGEAAGDVAMT
CHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHH
RLLLGLGLRSFSMHPAQILAVKQEVLRSDTRKLAPWARTVIDADNPADVLGG
HHHHHHCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1653223 [H]