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The map label for this gene is tuf1 [H]

Identifier: 120609039

GI number: 120609039

Start: 368694

End: 369884

Strand: Direct

Name: tuf1 [H]

Synonym: Aave_0336

Alternate gene names: 120609039

Gene position: 368694-369884 (Clockwise)

Preceding gene: 120609038

Following gene: 120609040

Centisome position: 6.89

GC content: 64.48

Gene sequence:

>1191_bases
ATGGCAAAAGGTAAGTTCGAACGTACCAAGCCCCACGTGAACGTGGGCACGATCGGCCACGTGGACCATGGCAAGACGAC
GCTGACGGCAGCGATCGCCACGGTGCTGTCCGCCAAGTTCGGCGGCGAAGCCAAGAAGTACGACGAAATCGACGCAGCGC
CCGAAGAAAAGGCCCGCGGCATCACCATCAACACCGCACACGTGGAATACGAGACGGCCAACCGCCACTACGCCCACGTG
GACTGCCCCGGCCACGCCGACTACGTCAAGAACATGATCACCGGTGCCGCCCAGATGGACGGCGCCATCCTGGTGTGCTC
GGCCGCCGACGGCCCGATGCCCCAGACGCGCGAGCACATCCTGCTGGCCCGCCAGGTGGGCGTGCCCTACATCATCGTGT
TCCTGAACAAGTGCGACATGGTGGATGACGAAGAGCTGCTCGAGCTCGTCGAGATGGAAGTGCGCGAACTCCTCGACAAG
TACGACTTCCCCGGCGACGACACCCCCATCGTGCGCGGCTCGGCCAAGCTGGCCCTCGAAGGCGACAAGGGCCCCCTGGG
CGAGCAAGCCATCGACAAGCTGGCCGAAGCCCTGGACACCTACATCCCGACGCCCGAGCGCGCCGTGGACGGTGCCTTCC
TGATGCCCGTGGAAGACGTGTTCTCGATCTCCGGTCGCGGTACCGTGGTGACGGGTCGTGTCGAGCGCGGCATCATCAAG
GTCGGCGAAGAAATCGAAATCGTGGGCATCCGCGACACGCAGAAGACCACCTGCACGGGCGTGGAAATGTTCCGCAAGCT
GCTGGACCAGGGCCAAGCCGGCGACAACGTCGGCCTGCTGCTGCGCGGCACGAAGCGTGAAGACGTCGAGCGCGGCCAGG
TGCTGTGCAAGCCCGGCTCCATCAAGCCGCACACCCACTTCACGGCCGAGGTGTACGTGCTGTCGAAGGACGAAGGCGGC
CGCCACACGCCCTTCTTCAACAACTACCGTCCGCAGTTCTACTTCCGCACGACCGACGTGACCGGCGCCATTGAGCTGCC
GGCCGACAAGGAAATGGTCATGCCCGGCGACAACGTGTCGATCACCGTCAAGCTGATCAACCCCATCGCCATGGAAGAAG
GCCTGCGCTTCGCCATCCGCGAAGGCGGCCGTACGGTCGGCGCCGGCGTCGTGGCCAAGATCATTGCTTAA

Upstream 100 bases:

>100_bases
GCGATCGCATGCCCTTCGTTGCCCGTGGCGAAGGAATCCGCAATGCGATGCAGACGTAAAACCAACACACGGGCATTGCT
CTTTATTCTGGAGTTGAGAA

Downstream 100 bases:

>100_bases
ATTCGAGCAATAAGGAATCTGCCATGTCCAAGCAAAAGATCCGTATCCGCCTGAAGGCTTTCGATTACAAGCTGATCGAC
CAGTCCGCCGCCGAGATCGT

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu 1 [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLSAKFGGEAKKYDEIDAAPEEKARGITINTAHVEYETANRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLDK
YDFPGDDTPIVRGSAKLALEGDKGPLGEQAIDKLAEALDTYIPTPERAVDGAFLMPVEDVFSISGRGTVVTGRVERGIIK
VGEEIEIVGIRDTQKTTCTGVEMFRKLLDQGQAGDNVGLLLRGTKREDVERGQVLCKPGSIKPHTHFTAEVYVLSKDEGG
RHTPFFNNYRPQFYFRTTDVTGAIELPADKEMVMPGDNVSITVKLINPIAMEEGLRFAIREGGRTVGAGVVAKIIA

Sequences:

>Translated_396_residues
MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLSAKFGGEAKKYDEIDAAPEEKARGITINTAHVEYETANRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLDK
YDFPGDDTPIVRGSAKLALEGDKGPLGEQAIDKLAEALDTYIPTPERAVDGAFLMPVEDVFSISGRGTVVTGRVERGIIK
VGEEIEIVGIRDTQKTTCTGVEMFRKLLDQGQAGDNVGLLLRGTKREDVERGQVLCKPGSIKPHTHFTAEVYVLSKDEGG
RHTPFFNNYRPQFYFRTTDVTGAIELPADKEMVMPGDNVSITVKLINPIAMEEGLRFAIREGGRTVGAGVVAKIIA
>Mature_395_residues
AKGKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLSAKFGGEAKKYDEIDAAPEEKARGITINTAHVEYETANRHYAHVD
CPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLDKY
DFPGDDTPIVRGSAKLALEGDKGPLGEQAIDKLAEALDTYIPTPERAVDGAFLMPVEDVFSISGRGTVVTGRVERGIIKV
GEEIEIVGIRDTQKTTCTGVEMFRKLLDQGQAGDNVGLLLRGTKREDVERGQVLCKPGSIKPHTHFTAEVYVLSKDEGGR
HTPFFNNYRPQFYFRTTDVTGAIELPADKEMVMPGDNVSITVKLINPIAMEEGLRFAIREGGRTVGAGVVAKIIA

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=395, Percent_Identity=57.4683544303797, Blast_Score=483, Evalue=1e-136,
Organism=Homo sapiens, GI223555963, Length=436, Percent_Identity=29.1284403669725, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI5729864, Length=436, Percent_Identity=29.1284403669725, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI4503475, Length=448, Percent_Identity=28.3482142857143, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI4503471, Length=442, Percent_Identity=29.185520361991, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI194018522, Length=434, Percent_Identity=28.5714285714286, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI194097354, Length=434, Percent_Identity=28.5714285714286, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI54607086, Length=332, Percent_Identity=29.2168674698795, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI194018520, Length=434, Percent_Identity=28.5714285714286, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI46094014, Length=435, Percent_Identity=28.735632183908, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI4503507, Length=275, Percent_Identity=28.3636363636364, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI94966754, Length=135, Percent_Identity=32.5925925925926, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI157426893, Length=290, Percent_Identity=25.1724137931034, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI53729339, Length=231, Percent_Identity=32.034632034632, Blast_Score=66, Evalue=7e-11,
Organism=Homo sapiens, GI53729337, Length=231, Percent_Identity=32.034632034632, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI1790412, Length=396, Percent_Identity=80.5555555555556, Blast_Score=629, Evalue=0.0,
Organism=Escherichia coli, GI1789737, Length=396, Percent_Identity=80.5555555555556, Blast_Score=628, Evalue=0.0,
Organism=Escherichia coli, GI2367247, Length=342, Percent_Identity=30.4093567251462, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI48994988, Length=243, Percent_Identity=30.8641975308642, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1789108, Length=319, Percent_Identity=28.5266457680251, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1788922, Length=334, Percent_Identity=25.4491017964072, Blast_Score=61, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI17556456, Length=403, Percent_Identity=52.3573200992556, Blast_Score=410, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=44.1860465116279, Blast_Score=332, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI17552884, Length=442, Percent_Identity=29.8642533936652, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17569207, Length=442, Percent_Identity=29.8642533936652, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI32566303, Length=434, Percent_Identity=28.3410138248848, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17506081, Length=304, Percent_Identity=29.9342105263158, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI32566629, Length=349, Percent_Identity=29.512893982808, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI115532067, Length=438, Percent_Identity=26.027397260274, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI115532065, Length=438, Percent_Identity=26.027397260274, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=38.7755102040816, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17509919, Length=268, Percent_Identity=26.4925373134328, Blast_Score=88, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17557151, Length=237, Percent_Identity=27.4261603375527, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=395, Percent_Identity=65.5696202531646, Blast_Score=530, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6325337, Length=440, Percent_Identity=29.7727272727273, Blast_Score=148, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6319594, Length=440, Percent_Identity=29.7727272727273, Blast_Score=148, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6320377, Length=339, Percent_Identity=30.0884955752212, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6322937, Length=358, Percent_Identity=26.8156424581006, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=268, Percent_Identity=27.6119402985075, Blast_Score=90, Evalue=6e-19,
Organism=Drosophila melanogaster, GI281363316, Length=394, Percent_Identity=59.6446700507614, Blast_Score=490, Evalue=1e-139,
Organism=Drosophila melanogaster, GI17864358, Length=394, Percent_Identity=59.6446700507614, Blast_Score=490, Evalue=1e-139,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=51.5544041450777, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24652838, Length=448, Percent_Identity=29.9107142857143, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI17137572, Length=448, Percent_Identity=29.9107142857143, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI45553807, Length=448, Percent_Identity=29.2410714285714, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI45553816, Length=448, Percent_Identity=29.2410714285714, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24651721, Length=448, Percent_Identity=29.2410714285714, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI17864154, Length=448, Percent_Identity=29.2410714285714, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI17137380, Length=344, Percent_Identity=30.5232558139535, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI19922690, Length=305, Percent_Identity=29.8360655737705, Blast_Score=121, Evalue=9e-28,
Organism=Drosophila melanogaster, GI45550900, Length=344, Percent_Identity=29.0697674418605, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24647046, Length=269, Percent_Identity=27.8810408921933, Blast_Score=95, Evalue=9e-20,
Organism=Drosophila melanogaster, GI24647048, Length=269, Percent_Identity=27.8810408921933, Blast_Score=95, Evalue=1e-19,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43129; Mature: 42998

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLSAKFGGEAKKYDEIDAAPEEKARG
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCC
ITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLDKYDFPGDDTPIVRGSAKLALE
HEEHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEEE
GDKGPLGEQAIDKLAEALDTYIPTPERAVDGAFLMPVEDVFSISGRGTVVTGRVERGIIK
CCCCCCCHHHHHHHHHHHHHHCCCCCHHCCCEEEEEHHHHHCCCCCCEEEECHHHCCCEE
VGEEIEIVGIRDTQKTTCTGVEMFRKLLDQGQAGDNVGLLLRGTKREDVERGQVLCKPGS
CCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCEEECCCC
IKPHTHFTAEVYVLSKDEGGRHTPFFNNYRPQFYFRTTDVTGAIELPADKEMVMPGDNVS
CCCCCEEEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCCEEECCCCCE
ITVKLINPIAMEEGLRFAIREGGRTVGAGVVAKIIA
EEEEEECCHHHHHCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKGKFERTKPHVNVGTIGHVDHGKTTLTAAIATVLSAKFGGEAKKYDEIDAAPEEKARG
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCC
ITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHH
LLARQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLDKYDFPGDDTPIVRGSAKLALE
HEEHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEEE
GDKGPLGEQAIDKLAEALDTYIPTPERAVDGAFLMPVEDVFSISGRGTVVTGRVERGIIK
CCCCCCCHHHHHHHHHHHHHHCCCCCHHCCCEEEEEHHHHHCCCCCCEEEECHHHCCCEE
VGEEIEIVGIRDTQKTTCTGVEMFRKLLDQGQAGDNVGLLLRGTKREDVERGQVLCKPGS
CCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCEEECCCC
IKPHTHFTAEVYVLSKDEGGRHTPFFNNYRPQFYFRTTDVTGAIELPADKEMVMPGDNVS
CCCCCEEEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCCEEECCCCCE
ITVKLINPIAMEEGLRFAIREGGRTVGAGVVAKIIA
EEEEEECCHHHHHCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA