Click here to switch to the map view.

The map label for this gene is clcB [H]

Identifier: 120608907

GI number: 120608907

Start: 223732

End: 224961

Strand: Direct

Name: clcB [H]

Synonym: Aave_0199

Alternate gene names: 120608907

Gene position: 223732-224961 (Clockwise)

Preceding gene: 120608904

Following gene: 120608908

Centisome position: 4.18

GC content: 72.44

Gene sequence:

>1230_bases
ATGACCGCAATCCCCTCCGCCGGCCTGCGCATCGAGCGCATCGAGGCGCGCATCCTCGACATCCCCACCATCCGGCCGCA
CAAGTTCTCGTTCGGAGCGATCCACCGCCAGAGCCCGGTGGTAGTGCAGCTGTGGCTGGCGGACGGCGCCTGCGGCTTCG
GCGAGGCCGCCACCATCGGCGGCCCCTCGTGGAACGAGGAATCGCCCGAGACCATCCTGCACGCCATCACGCAGTACCTC
GGCCCCGCCGTGGCCGGGCAGGACGCGGGCCGCTTCGAGCAGCTGCTGGCGCGCATGGACGCCGTCTGCAAGGGCAATCG
CTTCGCCAAGAGCGCCGTGGAAATGGCCGCCATCGACGCCGTGGCCCGTTCCCTGGGCCTGCCCGCCTGGCAGCTGCTGG
GCGGCAAGCAGCACGAAAGCCTGCCCCTGGCCTGGACGCTCGCCAGCGGCGATTCCGCCCGCGACGTGGAAGAGGCCGAG
CGCATGCTGGAGGCCCGCCGCCACCGCATCTTCAAGCTCAAGATCGGCGCGCGCGCCCCGCAGGACGACGTGGCCCATGT
CACCCGCATCGCCCGCGGCCTGGACGGGCGCGCCGGCATGACCGTGGACATCAACCAGGCCTGGGACGGCAACACCGCGC
GCCGCTACGTGCCGCAGCTCATCGATGCGGGCGTGAGCCTGATCGAGCAGCCCGTCGCGCAATGGAACGTGGAGGCCCTG
CGCCAGCTCACCGCCGGCCTGCCCGCCGGCGCGCTGGTGATGGCCGACGAAACCGTCTGCACGCCGCAGGACGCCATGCT
GCTGGCGCGGCAGGGCGCCTGCCATGTCTTCTCCCTGAAAGTCGCCAAGCACGGCGGCCTGCTGCGCACCCGCGCAGTCG
CCGCCGTGGCCGAGGCCGCGGACATCGGCTGGTACGGCGGCACCATGCTGGAAACGTCGCTGGGCAGCGCCGCATCGGCC
CACGTTTTCGCCACGCTGCCCGGCACGCACCACAACTGCGAGCTGTTCGGCCCGCAACTGCTGGTGGACGACATCGTCGC
CACGCCCATGACCGTGCGCGACTTCGCGCTGCAATTGCCCGATGGCCCCGGCTTCGGCGTGGAGGTGCTGCCCGAAGCAC
TGCGCCGTTTCGACCGCGCCCGCGAAGGCCTCGCGCCCGTGCACGTGGACATGGGCCGCCAGGCCGCCGCCCCGGCCGCG
GAGCCGGCGCCTGCCGCCCCCTCTGCCTGA

Upstream 100 bases:

>100_bases
ACGGGTGAATGGAAGGTGCGCATTATCGTTCGATGGCATCGAATGAATCGTGTCATATATACCATTGGATCGCAGGCTTT
CTCCGGGATAAGGTGCAGCC

Downstream 100 bases:

>100_bases
CGCCTGCGCCACCCCTTCATCCATCCAACCCTCATCGAACCTCAGAATCCGGAGACAAGCACCATGCCATCCATCCGCGC
CCACGCACTGCCGTCCGCCC

Product: muconate and chloromuconate cycloisomerase

Products: NA

Alternate protein names: Muconate cycloisomerase II [H]

Number of amino acids: Translated: 409; Mature: 408

Protein sequence:

>409_residues
MTAIPSAGLRIERIEARILDIPTIRPHKFSFGAIHRQSPVVVQLWLADGACGFGEAATIGGPSWNEESPETILHAITQYL
GPAVAGQDAGRFEQLLARMDAVCKGNRFAKSAVEMAAIDAVARSLGLPAWQLLGGKQHESLPLAWTLASGDSARDVEEAE
RMLEARRHRIFKLKIGARAPQDDVAHVTRIARGLDGRAGMTVDINQAWDGNTARRYVPQLIDAGVSLIEQPVAQWNVEAL
RQLTAGLPAGALVMADETVCTPQDAMLLARQGACHVFSLKVAKHGGLLRTRAVAAVAEAADIGWYGGTMLETSLGSAASA
HVFATLPGTHHNCELFGPQLLVDDIVATPMTVRDFALQLPDGPGFGVEVLPEALRRFDRAREGLAPVHVDMGRQAAAPAA
EPAPAAPSA

Sequences:

>Translated_409_residues
MTAIPSAGLRIERIEARILDIPTIRPHKFSFGAIHRQSPVVVQLWLADGACGFGEAATIGGPSWNEESPETILHAITQYL
GPAVAGQDAGRFEQLLARMDAVCKGNRFAKSAVEMAAIDAVARSLGLPAWQLLGGKQHESLPLAWTLASGDSARDVEEAE
RMLEARRHRIFKLKIGARAPQDDVAHVTRIARGLDGRAGMTVDINQAWDGNTARRYVPQLIDAGVSLIEQPVAQWNVEAL
RQLTAGLPAGALVMADETVCTPQDAMLLARQGACHVFSLKVAKHGGLLRTRAVAAVAEAADIGWYGGTMLETSLGSAASA
HVFATLPGTHHNCELFGPQLLVDDIVATPMTVRDFALQLPDGPGFGVEVLPEALRRFDRAREGLAPVHVDMGRQAAAPAA
EPAPAAPSA
>Mature_408_residues
TAIPSAGLRIERIEARILDIPTIRPHKFSFGAIHRQSPVVVQLWLADGACGFGEAATIGGPSWNEESPETILHAITQYLG
PAVAGQDAGRFEQLLARMDAVCKGNRFAKSAVEMAAIDAVARSLGLPAWQLLGGKQHESLPLAWTLASGDSARDVEEAER
MLEARRHRIFKLKIGARAPQDDVAHVTRIARGLDGRAGMTVDINQAWDGNTARRYVPQLIDAGVSLIEQPVAQWNVEALR
QLTAGLPAGALVMADETVCTPQDAMLLARQGACHVFSLKVAKHGGLLRTRAVAAVAEAADIGWYGGTMLETSLGSAASAH
VFATLPGTHHNCELFGPQLLVDDIVATPMTVRDFALQLPDGPGFGVEVLPEALRRFDRAREGLAPVHVDMGRQAAAPAAE
PAPAAPSA

Specific function: Highly active toward chlorinated substrates but retains diminished activity toward the non-chlorinated substrates [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013370
- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =5.5.1.7 [H]

Molecular weight: Translated: 43492; Mature: 43361

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAIPSAGLRIERIEARILDIPTIRPHKFSFGAIHRQSPVVVQLWLADGACGFGEAATIG
CCCCCCCCCEEEEEHHHEECCCCCCCCCCCCCCEECCCCEEEEEEEECCCCCCCCCCCCC
GPSWNEESPETILHAITQYLGPAVAGQDAGRFEQLLARMDAVCKGNRFAKSAVEMAAIDA
CCCCCCCCHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
VARSLGLPAWQLLGGKQHESLPLAWTLASGDSARDVEEAERMLEARRHRIFKLKIGARAP
HHHHHCCCHHHHCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCEEEEEEECCCCC
QDDVAHVTRIARGLDGRAGMTVDINQAWDGNTARRYVPQLIDAGVSLIEQPVAQWNVEAL
HHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
RQLTAGLPAGALVMADETVCTPQDAMLLARQGACHVFSLKVAKHGGLLRTRAVAAVAEAA
HHHHCCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEEEEEHHCCCHHHHHHHHHHHHHH
DIGWYGGTMLETSLGSAASAHVFATLPGTHHNCELFGPQLLVDDIVATPMTVRDFALQLP
HCCCCCCHHHHHHCCCCCCCEEEEECCCCCCCCEECCCHHHHHHHHCCCCCHHHHEEECC
DGPGFGVEVLPEALRRFDRAREGLAPVHVDMGRQAAAPAAEPAPAAPSA
CCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TAIPSAGLRIERIEARILDIPTIRPHKFSFGAIHRQSPVVVQLWLADGACGFGEAATIG
CCCCCCCCEEEEEHHHEECCCCCCCCCCCCCCEECCCCEEEEEEEECCCCCCCCCCCCC
GPSWNEESPETILHAITQYLGPAVAGQDAGRFEQLLARMDAVCKGNRFAKSAVEMAAIDA
CCCCCCCCHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
VARSLGLPAWQLLGGKQHESLPLAWTLASGDSARDVEEAERMLEARRHRIFKLKIGARAP
HHHHHCCCHHHHCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCEEEEEEECCCCC
QDDVAHVTRIARGLDGRAGMTVDINQAWDGNTARRYVPQLIDAGVSLIEQPVAQWNVEAL
HHHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
RQLTAGLPAGALVMADETVCTPQDAMLLARQGACHVFSLKVAKHGGLLRTRAVAAVAEAA
HHHHCCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEEEEEHHCCCHHHHHHHHHHHHHH
DIGWYGGTMLETSLGSAASAHVFATLPGTHHNCELFGPQLLVDDIVATPMTVRDFALQLP
HCCCCCCHHHHHHCCCCCCCEEEEECCCCCCCCEECCCHHHHHHHHCCCCCHHHHEEECC
DGPGFGVEVLPEALRRFDRAREGLAPVHVDMGRQAAAPAAEPAPAAPSA
CCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3299368; 3609743; 2583528 [H]