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The map label for this gene is bphA [H]

Identifier: 120608873

GI number: 120608873

Start: 184498

End: 185763

Strand: Direct

Name: bphA [H]

Synonym: Aave_0165

Alternate gene names: 120608873

Gene position: 184498-185763 (Clockwise)

Preceding gene: 120608872

Following gene: 120608874

Centisome position: 3.45

GC content: 65.8

Gene sequence:

>1266_bases
ATGAGCACGCCCCACCCCGTCTTCCCATTGCAGCCCGTCTGGGAGAGCCCCGGCACCCATCGCGTGCCGTTCGCGGCCTA
CACCAGCGAAGAGATCTACCGCCGCGAGCTGGAACGATTTTTCTACCGCGGCCACTGGTGCTACGTGGGGCTGGAGGCCG
AAGTGCCCAACCCCGGCGACTTCAAGCGCACGGCGATCGGCGAGCGCTCGGTGATCCTGGTGCGCGGCGAGGACATGCAG
TTGCACGTGGTGGAGAACGTCTGCGCGCACCGCGGCATGCGGTTTTGCCGCGAGCGGCACGGCAACCGCAAGGAGTTCGT
CTGCCCCTACCACCAGTGGAGCTACAGGCTCGATGGCGCGCTGCAGGGCGTGCCGTTCCGGCGCGGAGTGAAGCAGGACG
GCAAGGTGCATGGCGGCATGCCGGCGGATTTCAAGCTCGAGGAGCACAGCCTCACGCGGCTCAAGGTGGCCGTGCGCGGC
GGGGTGGTGTTCGCCTCTTTCGACCACGGCGTGGAATCGCTGGAGGACTTCATGGGCCCGGTGATCCTGGGCTATTTCGA
CCGCATGTTCAACGGCCGCAAGCTCAAGATCCTCGGCTACAACCGCCAGCGCATTCCGGGCAACTGGAAGCTGATGCAGG
AGAACATCAAGGACCCGTACCACCCCGGCCTGCTGCACACCTGGTTCGTCACCTTCGGCCTCTGGCGGGCGGACAACAAG
TCGGAATTGAAGATGGATGCGCGCCACCGCCATGCGGCCATGGTGTCCACGCGCGGCGAGGCCAAGAAGCAGTCGGACGT
GTCGAACGTTTCCAGCTTCAAGGCCGGCATGCAGCTGGAAGACCCGCGCTTTCTCGACATCGTGCCCGAGCCCTGGTGGG
GCGGCCCCACGGCGGTGATGACCACGCTGTTCCCGAGCGTGATCCTGCAGCAGCAGGTCAACAGCGTCTCCACGCGCCAC
ATCCAGCCCGTGGGCCACGATGCCTTCGATTTCGTGTGGACGCATTTCGGCTACGAGGACGACACCGACGAGATGACCGA
GCGCCGCCTGCGCCAGTCCAACCTGTTCGGGCCGGCGGGCTTCGTGTCGGCCGACGACGGCGAGGTGATCGAGTTCTCGC
AGCAGGGCTTCGAGCAGAAGCCCTTCCACCGCGCGCTGGTGGAACTGGGCGGCCACGAAGTGGGCGACACCGACCACATG
GTGACCGAGACGCTGATCCGCGGCATGTACGAATACTGGCGCGGCGTGATGGAGAAGGAGGACTGA

Upstream 100 bases:

>100_bases
GTGTCGCTGCTGGCGCGCCAGCGCGGCATCGCGCCCGAGCATCTCTATGCGGACGCTTTCTACGCCAGTACGCCCTGACA
CATCCCGAGGAGCCAACGAC

Downstream 100 bases:

>100_bases
GGCCATGAGCATGAAGACACCCATCGACTTCGCCGACTGGTTCGCGCTGCAGCAGCTCTATGCCGACTATGCCTCGGCGC
TGGACGCCGCCGACTGGGAC

Product: Rieske (2Fe-2S) domain-containing protein

Products: NA

Alternate protein names: Biphenyl 2,3-dioxygenase [H]

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MSTPHPVFPLQPVWESPGTHRVPFAAYTSEEIYRRELERFFYRGHWCYVGLEAEVPNPGDFKRTAIGERSVILVRGEDMQ
LHVVENVCAHRGMRFCRERHGNRKEFVCPYHQWSYRLDGALQGVPFRRGVKQDGKVHGGMPADFKLEEHSLTRLKVAVRG
GVVFASFDHGVESLEDFMGPVILGYFDRMFNGRKLKILGYNRQRIPGNWKLMQENIKDPYHPGLLHTWFVTFGLWRADNK
SELKMDARHRHAAMVSTRGEAKKQSDVSNVSSFKAGMQLEDPRFLDIVPEPWWGGPTAVMTTLFPSVILQQQVNSVSTRH
IQPVGHDAFDFVWTHFGYEDDTDEMTERRLRQSNLFGPAGFVSADDGEVIEFSQQGFEQKPFHRALVELGGHEVGDTDHM
VTETLIRGMYEYWRGVMEKED

Sequences:

>Translated_421_residues
MSTPHPVFPLQPVWESPGTHRVPFAAYTSEEIYRRELERFFYRGHWCYVGLEAEVPNPGDFKRTAIGERSVILVRGEDMQ
LHVVENVCAHRGMRFCRERHGNRKEFVCPYHQWSYRLDGALQGVPFRRGVKQDGKVHGGMPADFKLEEHSLTRLKVAVRG
GVVFASFDHGVESLEDFMGPVILGYFDRMFNGRKLKILGYNRQRIPGNWKLMQENIKDPYHPGLLHTWFVTFGLWRADNK
SELKMDARHRHAAMVSTRGEAKKQSDVSNVSSFKAGMQLEDPRFLDIVPEPWWGGPTAVMTTLFPSVILQQQVNSVSTRH
IQPVGHDAFDFVWTHFGYEDDTDEMTERRLRQSNLFGPAGFVSADDGEVIEFSQQGFEQKPFHRALVELGGHEVGDTDHM
VTETLIRGMYEYWRGVMEKED
>Mature_420_residues
STPHPVFPLQPVWESPGTHRVPFAAYTSEEIYRRELERFFYRGHWCYVGLEAEVPNPGDFKRTAIGERSVILVRGEDMQL
HVVENVCAHRGMRFCRERHGNRKEFVCPYHQWSYRLDGALQGVPFRRGVKQDGKVHGGMPADFKLEEHSLTRLKVAVRGG
VVFASFDHGVESLEDFMGPVILGYFDRMFNGRKLKILGYNRQRIPGNWKLMQENIKDPYHPGLLHTWFVTFGLWRADNKS
ELKMDARHRHAAMVSTRGEAKKQSDVSNVSSFKAGMQLEDPRFLDIVPEPWWGGPTAVMTTLFPSVILQQQVNSVSTRHI
QPVGHDAFDFVWTHFGYEDDTDEMTERRLRQSNLFGPAGFVSADDGEVIEFSQQGFEQKPFHRALVELGGHEVGDTDHMV
TETLIRGMYEYWRGVMEKED

Specific function: Part Of The Multicomponent 3-Phenylpropionate Dioxygenase. Converts 3-Phenylpropionic Acid (Pp) Into Cis-3-(3- Carboxyethyl)-3,5-Cyclohexadiene-1,2-Diol (Pp-Dihydrodiol). [C]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=468, Percent_Identity=27.7777777777778, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1788103, Length=209, Percent_Identity=29.1866028708134, Blast_Score=92, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: =1.14.12.18 [H]

Molecular weight: Translated: 48518; Mature: 48386

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS00570 RING_HYDROXYL_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPHPVFPLQPVWESPGTHRVPFAAYTSEEIYRRELERFFYRGHWCYVGLEAEVPNPGD
CCCCCCCCCCCHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCC
FKRTAIGERSVILVRGEDMQLHVVENVCAHRGMRFCRERHGNRKEFVCPYHQWSYRLDGA
CCHHHCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHHHHCCCCCCEEECHHHCCEEECCC
LQGVPFRRGVKQDGKVHGGMPADFKLEEHSLTRLKVAVRGGVVFASFDHGVESLEDFMGP
CCCCCHHCCCCCCCCCCCCCCCCCEECCCCCEEEEEEEECCEEEEECHHHHHHHHHHHHH
VILGYFDRMFNGRKLKILGYNRQRIPGNWKLMQENIKDPYHPGLLHTWFVTFGLWRADNK
HHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCH
SELKMDARHRHAAMVSTRGEAKKQSDVSNVSSFKAGMQLEDPRFLDIVPEPWWGGPTAVM
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCHHHHH
TTLFPSVILQQQVNSVSTRHIQPVGHDAFDFVWTHFGYEDDTDEMTERRLRQSNLFGPAG
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCC
FVSADDGEVIEFSQQGFEQKPFHRALVELGGHEVGDTDHMVTETLIRGMYEYWRGVMEKE
CCCCCCCCEEEEHHCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
D
C
>Mature Secondary Structure 
STPHPVFPLQPVWESPGTHRVPFAAYTSEEIYRRELERFFYRGHWCYVGLEAEVPNPGD
CCCCCCCCCCHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCC
FKRTAIGERSVILVRGEDMQLHVVENVCAHRGMRFCRERHGNRKEFVCPYHQWSYRLDGA
CCHHHCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHHHHCCCCCCEEECHHHCCEEECCC
LQGVPFRRGVKQDGKVHGGMPADFKLEEHSLTRLKVAVRGGVVFASFDHGVESLEDFMGP
CCCCCHHCCCCCCCCCCCCCCCCCEECCCCCEEEEEEEECCEEEEECHHHHHHHHHHHHH
VILGYFDRMFNGRKLKILGYNRQRIPGNWKLMQENIKDPYHPGLLHTWFVTFGLWRADNK
HHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCH
SELKMDARHRHAAMVSTRGEAKKQSDVSNVSSFKAGMQLEDPRFLDIVPEPWWGGPTAVM
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCHHHHH
TTLFPSVILQQQVNSVSTRHIQPVGHDAFDFVWTHFGYEDDTDEMTERRLRQSNLFGPAG
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCC
FVSADDGEVIEFSQQGFEQKPFHRALVELGGHEVGDTDHMVTETLIRGMYEYWRGVMEKE
CCCCCCCCEEEEHHCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
D
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1569021; 7592331 [H]