Click here to switch to the map view.

The map label for this gene is mhpC2 [H]

Identifier: 120608836

GI number: 120608836

Start: 138855

End: 139643

Strand: Direct

Name: mhpC2 [H]

Synonym: Aave_0128

Alternate gene names: 120608836

Gene position: 138855-139643 (Clockwise)

Preceding gene: 120608834

Following gene: 120608837

Centisome position: 2.59

GC content: 73.38

Gene sequence:

>789_bases
ATGGCCCTGCTTCCCACTTTCACCACCCTTGGCGCCGGCCCCACCGTGCTCATGCTGCACGATGCCGACGGCGGCCACCT
GACTTTCGCTCCCCAGGTCGAAACCCTCGCGGGCTCCGGCTACCGTGCCGTGGCCTGGGACATGCCGGGTTACGGCCGCA
GCGCGCCCATCGAGCCCTACAGCTTCAAGGGGCTGGCGCAGAGCTGCATCGCCCTGCTCGATGCCCTGCAGTGCACGCAG
GGCACGACCCTCGTCGGCCACGGCCTGGGCGCCATGGTGGCGGTCGAGGTCGCCTTGCGGGCGCCCCGCCGCGTGGGCCG
GCTCGTGCTGTGCGCTGGCGGCCCGGCCCTGGACGGCGAAGGCCTGGACACCTGGGTGGCGCCCCGCGTCGCCGCGCTGG
AGGACGACCAGGCCATGGAGCGCGTGGCCGAACTGCTGGTGCCGCGCCAGACCGGTACCGGGGCGTTGCCCGAAGGCGCG
CGCCTGGCCGGCCATGCGATGGGCCAGGTCCACCGTTCCGCCTTCCGCCGCGCGCTGGAATTGCTGCCGGCGTTCGCGCG
CGATGCCGCGGACCTCGCACACCTCTCCATGCCCACCCTGCTCGTGAGCGGCGCCCAGGACCGCTGCATGCCGCCCGAGG
CCCTGCAGGCCCTGGCCCACGTGCTGCCCGACGCGCGGCACCTGAGCCTGCCGAACGTGGGGCACTGGCCGCAACTGGAG
GATCCGGACGGTTTCGATGGCGCGCTGCTGGACTTCCTCGGCTCCTCCGCTCCGCGGACCATCCACTGA

Upstream 100 bases:

>100_bases
GGCGGAAGGAATGGGTCGGCTATTATGGTCCGGTGCCCGCGCCCCCGCCGCCGACCGGGGCCGAATCCCCACCGGGCGCG
TTGTCGTCGTCCCACCTTCC

Downstream 100 bases:

>100_bases
TGGCGGCGCGCACGAACGGCACTTCCGGGGTGCCGGCCATCCCGCGCCGTCCGGGGTTCCTTTCCCCATGGCTGATGCCC
CCGGCGGCCGCGCTGGCGCT

Product: alpha/beta hydrolase fold protein

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: 2-hydroxy-6-ketonona-2,4-diene-1,9-dioic acid 5,6-hydrolase 2; 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioic acid 5,6-hydrolase 2; 2-hydroxy-6-oxonona-2,4-diene-1,9-dioic acid 5,6-hydrolase 2 [H]

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MALLPTFTTLGAGPTVLMLHDADGGHLTFAPQVETLAGSGYRAVAWDMPGYGRSAPIEPYSFKGLAQSCIALLDALQCTQ
GTTLVGHGLGAMVAVEVALRAPRRVGRLVLCAGGPALDGEGLDTWVAPRVAALEDDQAMERVAELLVPRQTGTGALPEGA
RLAGHAMGQVHRSAFRRALELLPAFARDAADLAHLSMPTLLVSGAQDRCMPPEALQALAHVLPDARHLSLPNVGHWPQLE
DPDGFDGALLDFLGSSAPRTIH

Sequences:

>Translated_262_residues
MALLPTFTTLGAGPTVLMLHDADGGHLTFAPQVETLAGSGYRAVAWDMPGYGRSAPIEPYSFKGLAQSCIALLDALQCTQ
GTTLVGHGLGAMVAVEVALRAPRRVGRLVLCAGGPALDGEGLDTWVAPRVAALEDDQAMERVAELLVPRQTGTGALPEGA
RLAGHAMGQVHRSAFRRALELLPAFARDAADLAHLSMPTLLVSGAQDRCMPPEALQALAHVLPDARHLSLPNVGHWPQLE
DPDGFDGALLDFLGSSAPRTIH
>Mature_261_residues
ALLPTFTTLGAGPTVLMLHDADGGHLTFAPQVETLAGSGYRAVAWDMPGYGRSAPIEPYSFKGLAQSCIALLDALQCTQG
TTLVGHGLGAMVAVEVALRAPRRVGRLVLCAGGPALDGEGLDTWVAPRVAALEDDQAMERVAELLVPRQTGTGALPEGAR
LAGHAMGQVHRSAFRRALELLPAFARDAADLAHLSMPTLLVSGAQDRCMPPEALQALAHVLPDARHLSLPNVGHWPQLED
PDGFDGALLDFLGSSAPRTIH

Specific function: Catalyzes the cleavage of the C5-C6 bond of 2-hydroxy-6- oxononadienedioate and 2-hydroxy-6-oxononatrienedioate, a dienol ring fission product of the bacterial meta-cleavage pathway for degradation of phenylpropionic acid [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. MhpC family [H]

Homologues:

Organism=Escherichia coli, GI87081721, Length=265, Percent_Identity=27.9245283018868, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 27478; Mature: 27347

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLPTFTTLGAGPTVLMLHDADGGHLTFAPQVETLAGSGYRAVAWDMPGYGRSAPIEPY
CCCCCCHHHCCCCCEEEEEECCCCCEEEECCCHHHHCCCCCEEEEECCCCCCCCCCCCCC
SFKGLAQSCIALLDALQCTQGTTLVGHGLGAMVAVEVALRAPRRVGRLVLCAGGPALDGE
CHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHCHHHHCEEEEECCCCCCCCC
GLDTWVAPRVAALEDDQAMERVAELLVPRQTGTGALPEGARLAGHAMGQVHRSAFRRALE
CCCHHHCCHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LLPAFARDAADLAHLSMPTLLVSGAQDRCMPPEALQALAHVLPDARHLSLPNVGHWPQLE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
DPDGFDGALLDFLGSSAPRTIH
CCCCCCHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
ALLPTFTTLGAGPTVLMLHDADGGHLTFAPQVETLAGSGYRAVAWDMPGYGRSAPIEPY
CCCCCHHHCCCCCEEEEEECCCCCEEEECCCHHHHCCCCCEEEEECCCCCCCCCCCCCC
SFKGLAQSCIALLDALQCTQGTTLVGHGLGAMVAVEVALRAPRRVGRLVLCAGGPALDGE
CHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHCHHHHCEEEEECCCCCCCCC
GLDTWVAPRVAALEDDQAMERVAELLVPRQTGTGALPEGARLAGHAMGQVHRSAFRRALE
CCCHHHCCHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LLPAFARDAADLAHLSMPTLLVSGAQDRCMPPEALQALAHVLPDARHLSLPNVGHWPQLE
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
DPDGFDGALLDFLGSSAPRTIH
CCCCCCHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA