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The map label for this gene is gyrB [H]

Identifier: 120608717

GI number: 120608717

Start: 2850

End: 5483

Strand: Direct

Name: gyrB [H]

Synonym: Aave_0003

Alternate gene names: 120608717

Gene position: 2850-5483 (Clockwise)

Preceding gene: 120608716

Following gene: 120608721

Centisome position: 0.05

GC content: 66.97

Gene sequence:

>2634_bases
ATGACCGCTGAGAACACCCTGCCCGAGCCCACCCTTCCCACGGGCGACAGCCAAGCGCAGTCCGTCGCAGCCCCCGACTC
CGTGCCCGCCCAGGCCGCCGCGCCCAGCGACGGCTACGGCGAAGGCGCGATCCAGATCCTGGAAGGCCTGGAGGCCGTGC
GCAAGCGCCCCGGCATGTACATCGGCGACACGTCGGACGGCACGGGCCTGCACCACCTCGTGTTCGAAGTGGTGGACAAC
TCCATCGACGAGGCGCTGGCCGGCCACTGCGACGACATCGTCGTCACCATCCACTCCGACAACTCCATCAGCGTGGTGGA
CAACGGCCGCGGCATCCCCACCGGCGTGAAGATGGACGACAAGCACGAGCCCAAGCGCTCGGCCGCCGAGATCGCTCTCA
CCGAACTGCACGCGGGCGGCAAGTTCAACCAGAACAGCTACAAGGTCTCGGGCGGCCTGCACGGCGTGGGCGTCTCGTGC
GTGAACGCGCTCAGCAAGATGCTGCGCCTCACCGTGCGCCGCGACGGCAAGGTGCACCTGCTGGAATTCAGCCAGGGCTT
TGTGCAGAACCGCATCCTGGAGACGGTGAACGGCGTCGAGGTCTCGCCCATGCGCGTGACCGGCGAAACCGATAAGCGCG
GCACCGAAGTGCACTTCCTGCCCGACACCGAGATCTTCAAGGAGAACAACGACTTCCACTACGAGATCCTCGCCAAGCGC
CTGCGCGAGCTCTCGTTCCTGAACAACGGCGTGCGCATCCGCCTCAAGGACGAGCGCACCGGCAAGGAAGACGACTTCTC
CGGCGCGGGCGGCGTGATGGGCTTCGTGCAGTTCATCAACGCCAGCAAGAAGGTGTTGCACCCCAACGCCTTCCACGCCA
CGGGCAGCCGCCCCGCGGAGACCTACGGCGGCATCCCCGGCACCGAGATCGGCGTGGAAGTGGCCATGCAGTGGAACGAC
GGCTTCAACGAATCCGTGCTCTGCTTCACCAACAACATTCCACAGCGCGACGGCGGCACCCACCTGACCGGCCTGCGCGC
CGCCATGACGCGCGTCATCGGCAAGTACATCGAGCAGAATGAGCTGGCCAAGAAGGCCAAGGTCGAGGTGAGCGGCGACG
ACATGCGCGAAGGCCTGTGCTGCGTGCTCTCCGTGAAGGTGCCCGAGCCCAAGTTCAGCAGCCAGACCAAGGACAAGCTC
GTCTCCAGCGAGGTGCGCGCGCCCGTGGAAGACATCGTCGCCAAGGCCCTCACCGACTACCTGGAAGAGCGCCCCAACGA
CGCCAAGATCCTCTGCGGCAAGATCGTGGAAGCCGCCCGCGCCCGCGAGGCCGCGCGCAAGGCCCGCGAGATGACGCGCC
GCAAGGGCGTGCTCGACGGCATGGGCCTGCCCGGCAAGCTCGCCGACTGCCAGGAGAAGGACCCGGCCCTGAGCGAGATC
TACATCGTGGAGGGCGACTCCGCCGGCGGCTCCGCCAAGCAGGGCCGCGACCGGAAGTTCCAGGCCATCCTGCCGCTGCG
CGGCAAGATCCTGAATGTGGAGAAGGCGCGCTACGAGAAGCTGCTCACCTCCAACGAAATCCTCACGCTCATCACCGCCC
TGGGCACCGGCATCGGCAAGGCCAGCGCCGACGGCACCGGCAAGGGCGGCGCGGACGACTTCAACGTGGACAAGCTGCGC
TACCACCGCATCATCATCATGACCGATGCCGACGTGGACGGCGCGCACATCCGCACCCTGCTGCTCACCTTCTTCTACCG
CCAGATGCCCGAACTGGTCGAGCGCGGCCACATCTACATCGCCCAGCCGCCGCTCTACAAGGTGAAGAACGGCAAGGAAG
AGCTCTACCTGAAGGACGGCCCCGCGCTGGACACCTTCCTGCTGCGCGTGGCCCTGCGCGACGCCAGCGTGACCACCGGC
GGCGAACAGGCCCGCACCCTGGACGGAGAGACCCTGAGCACCCTGGCGCAGAAGCACCAGGTGGCCGAGGCCGTCATCGA
ACGCCTCTCCGCCTTCATGGACCGCGAGGCCCTGCGCGCCATCGCCGACGGCGTCGCGCTCAACCTGGACACCGTGGAAG
ACGCCGAAGCCAGCGCCGTCGCCCTGCAGGCCAAGCTGCGCGAACTCACCACCACCGGCGTGCCCGCCGAAGTGGCCGGC
GAATTCGACGCCCGCACCGACAAGCCCGTGCTGCGCATCAGCCGCCGCCACCACGGCAACATCAAGAGCAGCATCATCAC
GCAGGACTTCGTGCACGGCGCCGACTACGCAGCCCTGGCCGAAGCCGCCCACACGTTCCGCGGCCTGCTCGGCGAAGGCG
CCAAGGTGCTGCGCGGCGAAGGCGAACGCCAGCGCGAGGAGAAAGTGAGCGACTTCCGCCAGGCCATGGCCTGGCTCATC
AAGGAAGCCGAACGCACCACCAGCCGCCAGCGCTACAAGGGTCTGGGCGAGATGAACCCCGAACAGCTGTGGGAAACGAC
CATGGACCCTGAAGTGCGCCGCCTGCTGCGCGTGCAGATCGAGGACGCGATCGAGGCGGACCGGGTGTTCACGATGCTGA
TGGGGGATGAGGTCGAGCCGCGAAGGGACTTTATTGAGACAAATGCGTTGCGGGCGGGGAACATTGATGTCTAA

Upstream 100 bases:

>100_bases
TACGTCGTGATGCCCATGCGCATCTGACGCACCGGTCCCCTGACTTTCTTTCGACCCCACCGGAAGCCGCGCGCGGCTTC
CCCGGACCCCAAGGCCTTCC

Downstream 100 bases:

>100_bases
CGACATCGATCTTTGTTGTCTAAATAACGCTTAAAAACCGCGCTAGACAATATACAAATCAATAACTTAGGACAACGCCA
TGGTTAAACAACCCTGGTGG

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 877; Mature: 876

Protein sequence:

>877_residues
MTAENTLPEPTLPTGDSQAQSVAAPDSVPAQAAAPSDGYGEGAIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVVDN
SIDEALAGHCDDIVVTIHSDNSISVVDNGRGIPTGVKMDDKHEPKRSAAEIALTELHAGGKFNQNSYKVSGGLHGVGVSC
VNALSKMLRLTVRRDGKVHLLEFSQGFVQNRILETVNGVEVSPMRVTGETDKRGTEVHFLPDTEIFKENNDFHYEILAKR
LRELSFLNNGVRIRLKDERTGKEDDFSGAGGVMGFVQFINASKKVLHPNAFHATGSRPAETYGGIPGTEIGVEVAMQWND
GFNESVLCFTNNIPQRDGGTHLTGLRAAMTRVIGKYIEQNELAKKAKVEVSGDDMREGLCCVLSVKVPEPKFSSQTKDKL
VSSEVRAPVEDIVAKALTDYLEERPNDAKILCGKIVEAARAREAARKAREMTRRKGVLDGMGLPGKLADCQEKDPALSEI
YIVEGDSAGGSAKQGRDRKFQAILPLRGKILNVEKARYEKLLTSNEILTLITALGTGIGKASADGTGKGGADDFNVDKLR
YHRIIIMTDADVDGAHIRTLLLTFFYRQMPELVERGHIYIAQPPLYKVKNGKEELYLKDGPALDTFLLRVALRDASVTTG
GEQARTLDGETLSTLAQKHQVAEAVIERLSAFMDREALRAIADGVALNLDTVEDAEASAVALQAKLRELTTTGVPAEVAG
EFDARTDKPVLRISRRHHGNIKSSIITQDFVHGADYAALAEAAHTFRGLLGEGAKVLRGEGERQREEKVSDFRQAMAWLI
KEAERTTSRQRYKGLGEMNPEQLWETTMDPEVRRLLRVQIEDAIEADRVFTMLMGDEVEPRRDFIETNALRAGNIDV

Sequences:

>Translated_877_residues
MTAENTLPEPTLPTGDSQAQSVAAPDSVPAQAAAPSDGYGEGAIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVVDN
SIDEALAGHCDDIVVTIHSDNSISVVDNGRGIPTGVKMDDKHEPKRSAAEIALTELHAGGKFNQNSYKVSGGLHGVGVSC
VNALSKMLRLTVRRDGKVHLLEFSQGFVQNRILETVNGVEVSPMRVTGETDKRGTEVHFLPDTEIFKENNDFHYEILAKR
LRELSFLNNGVRIRLKDERTGKEDDFSGAGGVMGFVQFINASKKVLHPNAFHATGSRPAETYGGIPGTEIGVEVAMQWND
GFNESVLCFTNNIPQRDGGTHLTGLRAAMTRVIGKYIEQNELAKKAKVEVSGDDMREGLCCVLSVKVPEPKFSSQTKDKL
VSSEVRAPVEDIVAKALTDYLEERPNDAKILCGKIVEAARAREAARKAREMTRRKGVLDGMGLPGKLADCQEKDPALSEI
YIVEGDSAGGSAKQGRDRKFQAILPLRGKILNVEKARYEKLLTSNEILTLITALGTGIGKASADGTGKGGADDFNVDKLR
YHRIIIMTDADVDGAHIRTLLLTFFYRQMPELVERGHIYIAQPPLYKVKNGKEELYLKDGPALDTFLLRVALRDASVTTG
GEQARTLDGETLSTLAQKHQVAEAVIERLSAFMDREALRAIADGVALNLDTVEDAEASAVALQAKLRELTTTGVPAEVAG
EFDARTDKPVLRISRRHHGNIKSSIITQDFVHGADYAALAEAAHTFRGLLGEGAKVLRGEGERQREEKVSDFRQAMAWLI
KEAERTTSRQRYKGLGEMNPEQLWETTMDPEVRRLLRVQIEDAIEADRVFTMLMGDEVEPRRDFIETNALRAGNIDV
>Mature_876_residues
TAENTLPEPTLPTGDSQAQSVAAPDSVPAQAAAPSDGYGEGAIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVVDNS
IDEALAGHCDDIVVTIHSDNSISVVDNGRGIPTGVKMDDKHEPKRSAAEIALTELHAGGKFNQNSYKVSGGLHGVGVSCV
NALSKMLRLTVRRDGKVHLLEFSQGFVQNRILETVNGVEVSPMRVTGETDKRGTEVHFLPDTEIFKENNDFHYEILAKRL
RELSFLNNGVRIRLKDERTGKEDDFSGAGGVMGFVQFINASKKVLHPNAFHATGSRPAETYGGIPGTEIGVEVAMQWNDG
FNESVLCFTNNIPQRDGGTHLTGLRAAMTRVIGKYIEQNELAKKAKVEVSGDDMREGLCCVLSVKVPEPKFSSQTKDKLV
SSEVRAPVEDIVAKALTDYLEERPNDAKILCGKIVEAARAREAARKAREMTRRKGVLDGMGLPGKLADCQEKDPALSEIY
IVEGDSAGGSAKQGRDRKFQAILPLRGKILNVEKARYEKLLTSNEILTLITALGTGIGKASADGTGKGGADDFNVDKLRY
HRIIIMTDADVDGAHIRTLLLTFFYRQMPELVERGHIYIAQPPLYKVKNGKEELYLKDGPALDTFLLRVALRDASVTTGG
EQARTLDGETLSTLAQKHQVAEAVIERLSAFMDREALRAIADGVALNLDTVEDAEASAVALQAKLRELTTTGVPAEVAGE
FDARTDKPVLRISRRHHGNIKSSIITQDFVHGADYAALAEAAHTFRGLLGEGAKVLRGEGERQREEKVSDFRQAMAWLIK
EAERTTSRQRYKGLGEMNPEQLWETTMDPEVRRLLRVQIEDAIEADRVFTMLMGDEVEPRRDFIETNALRAGNIDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=595, Percent_Identity=24.3697478991597, Blast_Score=121, Evalue=3e-27,
Organism=Homo sapiens, GI19913406, Length=589, Percent_Identity=24.1086587436333, Blast_Score=113, Evalue=9e-25,
Organism=Escherichia coli, GI48994957, Length=848, Percent_Identity=57.0754716981132, Blast_Score=920, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=582, Percent_Identity=38.1443298969072, Blast_Score=367, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17535065, Length=621, Percent_Identity=24.6376811594203, Blast_Score=129, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI212645845, Length=542, Percent_Identity=24.5387453874539, Blast_Score=104, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI212645657, Length=168, Percent_Identity=32.1428571428571, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324241, Length=631, Percent_Identity=25.1980982567353, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI17136538, Length=574, Percent_Identity=23.8675958188153, Blast_Score=111, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 95966; Mature: 95835

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAENTLPEPTLPTGDSQAQSVAAPDSVPAQAAAPSDGYGEGAIQILEGLEAVRKRPGMY
CCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEE
IGDTSDGTGLHHLVFEVVDNSIDEALAGHCDDIVVTIHSDNSISVVDNGRGIPTGVKMDD
EECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEEECCCCCCCCCCCCC
KHEPKRSAAEIALTELHAGGKFNQNSYKVSGGLHGVGVSCVNALSKMLRLTVRRDGKVHL
CCCCHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEE
LEFSQGFVQNRILETVNGVEVSPMRVTGETDKRGTEVHFLPDTEIFKENNDFHYEILAKR
EECHHHHHHHHHHHHHCCCEECEEEEECCCCCCCCEEEECCCHHHHCCCCCCHHHHHHHH
LRELSFLNNGVRIRLKDERTGKEDDFSGAGGVMGFVQFINASKKVLHPNAFHATGSRPAE
HHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCHHHCCCCCCCCCCCCCHH
TYGGIPGTEIGVEVAMQWNDGFNESVLCFTNNIPQRDGGTHLTGLRAAMTRVIGKYIEQN
HHCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ELAKKAKVEVSGDDMREGLCCVLSVKVPEPKFSSQTKDKLVSSEVRAPVEDIVAKALTDY
HHHHHHEEEECCCHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
LEERPNDAKILCGKIVEAARAREAARKAREMTRRKGVLDGMGLPGKLADCQEKDPALSEI
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEE
YIVEGDSAGGSAKQGRDRKFQAILPLRGKILNVEKARYEKLLTSNEILTLITALGTGIGK
EEEECCCCCCCCCCCCCCCEEEECCCCCCEECHHHHHHHHHHCCHHHHHHHHHHHCCCCC
ASADGTGKGGADDFNVDKLRYHRIIIMTDADVDGAHIRTLLLTFFYRQMPELVERGHIYI
CCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEE
AQPPLYKVKNGKEELYLKDGPALDTFLLRVALRDASVTTGGEQARTLDGETLSTLAQKHQ
ECCCCEECCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHHH
VAEAVIERLSAFMDREALRAIADGVALNLDTVEDAEASAVALQAKLRELTTTGVPAEVAG
HHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCHHHCC
EFDARTDKPVLRISRRHHGNIKSSIITQDFVHGADYAALAEAAHTFRGLLGEGAKVLRGE
CCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHCCC
GERQREEKVSDFRQAMAWLIKEAERTTSRQRYKGLGEMNPEQLWETTMDPEVRRLLRVQI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHH
EDAIEADRVFTMLMGDEVEPRRDFIETNALRAGNIDV
HHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TAENTLPEPTLPTGDSQAQSVAAPDSVPAQAAAPSDGYGEGAIQILEGLEAVRKRPGMY
CCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEE
IGDTSDGTGLHHLVFEVVDNSIDEALAGHCDDIVVTIHSDNSISVVDNGRGIPTGVKMDD
EECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEEEEECCCCCCCCCCCCC
KHEPKRSAAEIALTELHAGGKFNQNSYKVSGGLHGVGVSCVNALSKMLRLTVRRDGKVHL
CCCCHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEE
LEFSQGFVQNRILETVNGVEVSPMRVTGETDKRGTEVHFLPDTEIFKENNDFHYEILAKR
EECHHHHHHHHHHHHHCCCEECEEEEECCCCCCCCEEEECCCHHHHCCCCCCHHHHHHHH
LRELSFLNNGVRIRLKDERTGKEDDFSGAGGVMGFVQFINASKKVLHPNAFHATGSRPAE
HHHHHHHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCHHHCCCCCCCCCCCCCHH
TYGGIPGTEIGVEVAMQWNDGFNESVLCFTNNIPQRDGGTHLTGLRAAMTRVIGKYIEQN
HHCCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ELAKKAKVEVSGDDMREGLCCVLSVKVPEPKFSSQTKDKLVSSEVRAPVEDIVAKALTDY
HHHHHHEEEECCCHHCCCEEEEEEEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
LEERPNDAKILCGKIVEAARAREAARKAREMTRRKGVLDGMGLPGKLADCQEKDPALSEI
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEE
YIVEGDSAGGSAKQGRDRKFQAILPLRGKILNVEKARYEKLLTSNEILTLITALGTGIGK
EEEECCCCCCCCCCCCCCCEEEECCCCCCEECHHHHHHHHHHCCHHHHHHHHHHHCCCCC
ASADGTGKGGADDFNVDKLRYHRIIIMTDADVDGAHIRTLLLTFFYRQMPELVERGHIYI
CCCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEE
AQPPLYKVKNGKEELYLKDGPALDTFLLRVALRDASVTTGGEQARTLDGETLSTLAQKHQ
ECCCCEECCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHHH
VAEAVIERLSAFMDREALRAIADGVALNLDTVEDAEASAVALQAKLRELTTTGVPAEVAG
HHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCHHHCC
EFDARTDKPVLRISRRHHGNIKSSIITQDFVHGADYAALAEAAHTFRGLLGEGAKVLRGE
CCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHCCC
GERQREEKVSDFRQAMAWLIKEAERTTSRQRYKGLGEMNPEQLWETTMDPEVRRLLRVQI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHH
EDAIEADRVFTMLMGDEVEPRRDFIETNALRAGNIDV
HHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]