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The map label for this gene is dnaN [H]

Identifier: 120608716

GI number: 120608716

Start: 1658

End: 2776

Strand: Direct

Name: dnaN [H]

Synonym: Aave_0002

Alternate gene names: 120608716

Gene position: 1658-2776 (Clockwise)

Preceding gene: 120608715

Following gene: 120608717

Centisome position: 0.03

GC content: 64.61

Gene sequence:

>1119_bases
ATGATCGTCCTGAAGGCAACACAAGACAAGGTTCTCGCGGTACTGCAATCGGTCGCGGGCATCGTCGAGCGCCGGCACAC
GCTGCCCATCCTGGCCAACGTGCTGATCCGCAAGACCGGCAATGCCCTGCAGCTCACCACCAGCGACCTGGAAATCCAGA
TCCGCACCACCGCGGAACTCGGTGGCGACACGGGCGACTTCACCACCACCGTGGGCGCGCGCAAGCTCATCGACATCCTC
AAGACCATGCCCGCCGACCAGACGGTGAGCCTGGAGTCGCAGCAGTCCAAGCTGGTGCTCAAGGGCGGCAAGAGCCGCTT
CACCCTGCAGTCGCTGCCCGCGGAAGACTTCCCCCTGGTGCAGGAATCCGCCGCCTTCGGCCCCGCCTTCAGCGTGCCGC
AGAAGGTGCTCAAGGACCTGCTCGGCCAGGTGTCGTTCGCCATGGCGGTGCAGGACATCCGCTACTACCTCAACGGCATC
CTGTTCGTGGCCGAGGGCAACACGCTGAGCCTGGTCGCCACCGACGGCCACCGCCTCGCCTTCGCCAGCGCCACGCTGGA
TGTGGAAGTGCCCAAGCAGGAAGTCATCCTGCCGCGCAAGACCGTGCTGGAACTGCAGCGCCTGCTGTCGGACGCCGGCG
GCGAGAACCAGCCCCACATCGAGATGCAGTTCGCCAACAACCAGGCGAAATTCACCTTCGGCGGCATGGAGTTCGTCACC
AAGCTGGTCGAGGGCAAGTTCCCCGACTACAACCGCGTCATCCCCAAGAACCACAAGAACAGCGTCACCCTGGGCCGCGC
GCCCCTGCTGGCCAGCCTGCAGCGCACGGCCATCATGACCAGCGACAAGTTCAAGGGCGTGCGCCTGAACATCGAACCCG
GCACCCTGCGCGTGGCGTCCAACAACGCCGAGCAGGAAGAGGCCGTGGACGAGCTCGACATCGACTACGGCGGCGACACC
ATCGAGATCGGCTTCAACGTCACCTACCTCATCGACGCGCTCGCGAACATGGGCCAGGACATGGTGCGCGTGGACCTCTC
CGACGGCAACAGCTCCGCGCTGATGACCATCCCCGAGAACGACAACTTCAAGTACGTCGTGATGCCCATGCGCATCTGA

Upstream 100 bases:

>100_bases
GGCAAAATGCCAGGTTGGCCGAGGGGTGCCGGCAAAGGCATCGCCCACGCGGCCGCCGGGCGGCCCGGCACCGTCGCACA
AATCACTAGGGGTTGAAGAC

Downstream 100 bases:

>100_bases
CGCACCGGTCCCCTGACTTTCTTTCGACCCCACCGGAAGCCGCGCGCGGCTTCCCCGGACCCCAAGGCCTTCCATGACCG
CTGAGAACACCCTGCCCGAG

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MIVLKATQDKVLAVLQSVAGIVERRHTLPILANVLIRKTGNALQLTTSDLEIQIRTTAELGGDTGDFTTTVGARKLIDIL
KTMPADQTVSLESQQSKLVLKGGKSRFTLQSLPAEDFPLVQESAAFGPAFSVPQKVLKDLLGQVSFAMAVQDIRYYLNGI
LFVAEGNTLSLVATDGHRLAFASATLDVEVPKQEVILPRKTVLELQRLLSDAGGENQPHIEMQFANNQAKFTFGGMEFVT
KLVEGKFPDYNRVIPKNHKNSVTLGRAPLLASLQRTAIMTSDKFKGVRLNIEPGTLRVASNNAEQEEAVDELDIDYGGDT
IEIGFNVTYLIDALANMGQDMVRVDLSDGNSSALMTIPENDNFKYVVMPMRI

Sequences:

>Translated_372_residues
MIVLKATQDKVLAVLQSVAGIVERRHTLPILANVLIRKTGNALQLTTSDLEIQIRTTAELGGDTGDFTTTVGARKLIDIL
KTMPADQTVSLESQQSKLVLKGGKSRFTLQSLPAEDFPLVQESAAFGPAFSVPQKVLKDLLGQVSFAMAVQDIRYYLNGI
LFVAEGNTLSLVATDGHRLAFASATLDVEVPKQEVILPRKTVLELQRLLSDAGGENQPHIEMQFANNQAKFTFGGMEFVT
KLVEGKFPDYNRVIPKNHKNSVTLGRAPLLASLQRTAIMTSDKFKGVRLNIEPGTLRVASNNAEQEEAVDELDIDYGGDT
IEIGFNVTYLIDALANMGQDMVRVDLSDGNSSALMTIPENDNFKYVVMPMRI
>Mature_372_residues
MIVLKATQDKVLAVLQSVAGIVERRHTLPILANVLIRKTGNALQLTTSDLEIQIRTTAELGGDTGDFTTTVGARKLIDIL
KTMPADQTVSLESQQSKLVLKGGKSRFTLQSLPAEDFPLVQESAAFGPAFSVPQKVLKDLLGQVSFAMAVQDIRYYLNGI
LFVAEGNTLSLVATDGHRLAFASATLDVEVPKQEVILPRKTVLELQRLLSDAGGENQPHIEMQFANNQAKFTFGGMEFVT
KLVEGKFPDYNRVIPKNHKNSVTLGRAPLLASLQRTAIMTSDKFKGVRLNIEPGTLRVASNNAEQEEAVDELDIDYGGDT
IEIGFNVTYLIDALANMGQDMVRVDLSDGNSSALMTIPENDNFKYVVMPMRI

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=365, Percent_Identity=40.2739726027397, Blast_Score=274, Evalue=7e-75,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40680; Mature: 40680

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVLKATQDKVLAVLQSVAGIVERRHTLPILANVLIRKTGNALQLTTSDLEIQIRTTAEL
CEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCEEEEEEEEHCC
GGDTGDFTTTVGARKLIDILKTMPADQTVSLESQQSKLVLKGGKSRFTLQSLPAEDFPLV
CCCCCCCEEHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEECCCCEEEEECCCCCCCCCH
QESAAFGPAFSVPQKVLKDLLGQVSFAMAVQDIRYYLNGILFVAEGNTLSLVATDGHRLA
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCEEEEEEECCCEEE
FASATLDVEVPKQEVILPRKTVLELQRLLSDAGGENQPHIEMQFANNQAKFTFGGMEFVT
EEEEEEEEECCHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEECHHHHHH
KLVEGKFPDYNRVIPKNHKNSVTLGRAPLLASLQRTAIMTSDKFKGVRLNIEPGTLRVAS
HHHCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHEEECCCCCCEEEEECCCEEEEEC
NNAEQEEAVDELDIDYGGDTIEIGFNVTYLIDALANMGQDMVRVDLSDGNSSALMTIPEN
CCCHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHCCCEEEEEEECCCCCCEEEECCCC
DNFKYVVMPMRI
CCEEEEEEEECC
>Mature Secondary Structure
MIVLKATQDKVLAVLQSVAGIVERRHTLPILANVLIRKTGNALQLTTSDLEIQIRTTAEL
CEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCEEEEEEEEHCC
GGDTGDFTTTVGARKLIDILKTMPADQTVSLESQQSKLVLKGGKSRFTLQSLPAEDFPLV
CCCCCCCEEHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEECCCCEEEEECCCCCCCCCH
QESAAFGPAFSVPQKVLKDLLGQVSFAMAVQDIRYYLNGILFVAEGNTLSLVATDGHRLA
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCEEEEEEECCCEEE
FASATLDVEVPKQEVILPRKTVLELQRLLSDAGGENQPHIEMQFANNQAKFTFGGMEFVT
EEEEEEEEECCHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEECHHHHHH
KLVEGKFPDYNRVIPKNHKNSVTLGRAPLLASLQRTAIMTSDKFKGVRLNIEPGTLRVAS
HHHCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHEEECCCCCCEEEEECCCEEEEEC
NNAEQEEAVDELDIDYGGDTIEIGFNVTYLIDALANMGQDMVRVDLSDGNSSALMTIPEN
CCCHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHCCCEEEEEEECCCCCCEEEECCCC
DNFKYVVMPMRI
CCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]