Click here to switch to the map view.

The map label for this gene is fruR [H]

Identifier: 119947152

GI number: 119947152

Start: 4367303

End: 4368286

Strand: Reverse

Name: fruR [H]

Synonym: Ping_3550

Alternate gene names: 119947152

Gene position: 4368286-4367303 (Counterclockwise)

Preceding gene: 119947158

Following gene: 119947148

Centisome position: 95.8

GC content: 41.77

Gene sequence:

>984_bases
ATGAAATTAGTAGAAATAGCGCGCTTAGCAGGTGTCTCTCGTACCACGGCCAGTTACGTTATTAATGGTAAAGCAGAGCA
ATATAGGATCAGTGAAAAAACGCGTCTGAAAGTGATGGCCGTTGTGGATAAATATAATTATAAACCTAACTATGCGGCCA
GTACTCTACGGGTGGGCAATAGCCGTACCTTTGGTTTAATTATCCCGGATCTGGAAAATACCAGTTACGCTAAAATCGCT
AAATTATTAGAGCGCGATGCCAGAAAATCGGGTTTTCAATTAATAATCTGTGGTTCGGGTGATGACATTAATACGGAAAT
GAATGTTGCTGAAAATCTTGTCCGGCGGGGTATTGATGTGTTAATTGTTGCCACTGCATTACCCGGTGATAATGAATTTT
ATCGCAATATACAGGCTAAAGGAGTGCCGGTCGTGGCGATAGACAGGTGTTTGGATGATGAGTGTTTCAGCAGCGTTATT
AGTGAAGATCTTGAAGGCGCTTATCAGTTATGTGAAGCCCTATTAGAAAAAAATATTAACAGTATTGGTTTAATTGGCGC
TGGGCCACTGCTGGGCGTCTCCAAAGAGCGAGAATTAGGGTTTAAAGCTGCACTGGCACAATACAGTGGCTCGCTTAACA
TTCAATACGCTTATGGTGAAGATTTTAGTATTGAACAAGGTCAATATTTAGCGCAGCAATGGATAGATCAGGGTTGTTTC
CCCGATGCCATTTTAATTACCTCTTATACATTATTTGTTGGAGTACTGGATTGTTTATTAGCACATCCCGAATTACAGGA
AAAAACTGAACTGGCAACTTTTGGTGATAACCGCCTGTTAGATTTTTTACCTATCAAAGTACAGTCCTTACCGCAGCAGT
TAGAAGTTATTGCAGAAAAAGCACTGCAACTGGCCTTGCTTGCTGCACAGGGAACTTATAATCCCAGTGTGGAAGTTGTC
CCGCGCCGGTTGATTAAACGCTAG

Upstream 100 bases:

>100_bases
TTTCAGCTACAATTGCAACTGCTTTTTGATGGTTATTGGTTTGGTGATTTCCTATACTGTGATACGGATCTCGATAATTG
TTTTTTATAAAGGATTTTTG

Downstream 100 bases:

>100_bases
TGCAGCGCAAATAATAAGGTGTGCGGTTAACTACATATTGAAAAAACCTAAAAATTCACACCATTGTGACGATTTTTCTT
CAATATCTGCATCGTAAAAG

Product: DNA-binding transcriptional regulator FruR

Products: NA

Alternate protein names: Catabolite repressor/activator [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MKLVEIARLAGVSRTTASYVINGKAEQYRISEKTRLKVMAVVDKYNYKPNYAASTLRVGNSRTFGLIIPDLENTSYAKIA
KLLERDARKSGFQLIICGSGDDINTEMNVAENLVRRGIDVLIVATALPGDNEFYRNIQAKGVPVVAIDRCLDDECFSSVI
SEDLEGAYQLCEALLEKNINSIGLIGAGPLLGVSKERELGFKAALAQYSGSLNIQYAYGEDFSIEQGQYLAQQWIDQGCF
PDAILITSYTLFVGVLDCLLAHPELQEKTELATFGDNRLLDFLPIKVQSLPQQLEVIAEKALQLALLAAQGTYNPSVEVV
PRRLIKR

Sequences:

>Translated_327_residues
MKLVEIARLAGVSRTTASYVINGKAEQYRISEKTRLKVMAVVDKYNYKPNYAASTLRVGNSRTFGLIIPDLENTSYAKIA
KLLERDARKSGFQLIICGSGDDINTEMNVAENLVRRGIDVLIVATALPGDNEFYRNIQAKGVPVVAIDRCLDDECFSSVI
SEDLEGAYQLCEALLEKNINSIGLIGAGPLLGVSKERELGFKAALAQYSGSLNIQYAYGEDFSIEQGQYLAQQWIDQGCF
PDAILITSYTLFVGVLDCLLAHPELQEKTELATFGDNRLLDFLPIKVQSLPQQLEVIAEKALQLALLAAQGTYNPSVEVV
PRRLIKR
>Mature_327_residues
MKLVEIARLAGVSRTTASYVINGKAEQYRISEKTRLKVMAVVDKYNYKPNYAASTLRVGNSRTFGLIIPDLENTSYAKIA
KLLERDARKSGFQLIICGSGDDINTEMNVAENLVRRGIDVLIVATALPGDNEFYRNIQAKGVPVVAIDRCLDDECFSSVI
SEDLEGAYQLCEALLEKNINSIGLIGAGPLLGVSKERELGFKAALAQYSGSLNIQYAYGEDFSIEQGQYLAQQWIDQGCF
PDAILITSYTLFVGVLDCLLAHPELQEKTELATFGDNRLLDFLPIKVQSLPQQLEVIAEKALQLALLAAQGTYNPSVEVV
PRRLIKR

Specific function: Represses some operons but activates others. It is implicated in the regulation of a large number of operons encoding enzymes which comprise central pathways of carbon metabolism. Binds D-fructose as an inducer [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786268, Length=330, Percent_Identity=44.2424242424242, Blast_Score=265, Evalue=3e-72,
Organism=Escherichia coli, GI1790194, Length=306, Percent_Identity=26.4705882352941, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1787948, Length=334, Percent_Identity=25.4491017964072, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787580, Length=251, Percent_Identity=23.9043824701195, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1787906, Length=337, Percent_Identity=24.6290801186944, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789068, Length=262, Percent_Identity=23.6641221374046, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1789202, Length=204, Percent_Identity=27.4509803921569, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1788474, Length=206, Percent_Identity=27.1844660194175, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790369, Length=257, Percent_Identity=23.7354085603113, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI48994940, Length=189, Percent_Identity=26.4550264550265, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012781
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36062; Mature: 36062

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLVEIARLAGVSRTTASYVINGKAEQYRISEKTRLKVMAVVDKYNYKPNYAASTLRVGN
CCHHHHHHHHCCCCCCHHEEECCCHHHEECCCHHHEEEEEEEECCCCCCCCCHHEEEECC
SRTFGLIIPDLENTSYAKIAKLLERDARKSGFQLIICGSGDDINTEMNVAENLVRRGIDV
CCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCCEE
LIVATALPGDNEFYRNIQAKGVPVVAIDRCLDDECFSSVISEDLEGAYQLCEALLEKNIN
EEEEEECCCCHHHHHHHHCCCCCEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SIGLIGAGPLLGVSKERELGFKAALAQYSGSLNIQYAYGEDFSIEQGQYLAQQWIDQGCF
CEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCC
PDAILITSYTLFVGVLDCLLAHPELQEKTELATFGDNRLLDFLPIKVQSLPQQLEVIAEK
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHCCHHHHHHHHHHHHHHHH
ALQLALLAAQGTYNPSVEVVPRRLIKR
HHHHHHHHCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKLVEIARLAGVSRTTASYVINGKAEQYRISEKTRLKVMAVVDKYNYKPNYAASTLRVGN
CCHHHHHHHHCCCCCCHHEEECCCHHHEECCCHHHEEEEEEEECCCCCCCCCHHEEEECC
SRTFGLIIPDLENTSYAKIAKLLERDARKSGFQLIICGSGDDINTEMNVAENLVRRGIDV
CCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHCCCEE
LIVATALPGDNEFYRNIQAKGVPVVAIDRCLDDECFSSVISEDLEGAYQLCEALLEKNIN
EEEEEECCCCHHHHHHHHCCCCCEEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SIGLIGAGPLLGVSKERELGFKAALAQYSGSLNIQYAYGEDFSIEQGQYLAQQWIDQGCF
CEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCC
PDAILITSYTLFVGVLDCLLAHPELQEKTELATFGDNRLLDFLPIKVQSLPQQLEVIAEK
CCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHCCHHHHHHHHHHHHHHHH
ALQLALLAAQGTYNPSVEVVPRRLIKR
HHHHHHHHCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]