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The map label for this gene is pyrE [H]

Identifier: 119947080

GI number: 119947080

Start: 4302467

End: 4303108

Strand: Reverse

Name: pyrE [H]

Synonym: Ping_3478

Alternate gene names: 119947080

Gene position: 4303108-4302467 (Counterclockwise)

Preceding gene: 119947081

Following gene: 119947079

Centisome position: 94.37

GC content: 43.15

Gene sequence:

>642_bases
ATGAAAGATTATCAAAAAGAATTTATTGAATTTGCCTTAGAAAAACAGGTTTTAAAGTTTGGCCAGTTTACCCTGAAATC
GGGCCGTACCAGCCCTTATTTTTTTAATGCCGGGTTGTTTAATAGTGGACGTGATTTAGCCCGTTTAGGGCGCTTTTATG
CGGCCGCTTTAATGGACTCAGGCATTGAGTATGACTTGCTTTTTGGTCCTGCCTATAAAGGTATTCCTATTGCAACCACA
ACAGCGGTTGCATTGTCTAACGATTACGATTTAGATATCCCCTACTGCTTTAACCGCAAAGAAGCTAAAACCCACGGCGA
AGGGGGGAGTTTAGTCGGATCAAAGCTGCAGGGTAAAATAATGCTGGTTGATGATGTGATCACCGCAGGCACTGCAATTC
GTGAGTCAATGGATATTATCCAAGCGCATCATGCCCAATTAGCGGGTGTTTTAATTGCATTGGATCGACAGGAAAAGGGC
CGCGCTGAACTGTCTGCTATTCAGGAAGTAGAGCGCGACTACGGTGCTAAAGTTATTTCTATTGTTAAGCTTAGTGACTT
AATTACTTATCTGGAAGCAAAGCCTGAAATGCAGAAGGATCTTGCTGCGGTTAACACCTATCGGGAACAATACGGCATTT
GA

Upstream 100 bases:

>100_bases
TTAGTCATGAACAGCTGCTTAGCATGTTAAGTCTTGCCAAGGGCGGAATTGAAGCTCTTGTTGTGGAACAGAAAAAAGCA
CTCAACGCGGAATAAAAAAA

Downstream 100 bases:

>100_bases
TTTTATCTCTGCCGGAAAAGCAAAAAAACCGCATTCTATTGCCAATAGACTGCGGTTTTTTTTAATAGATCTATCTTAAT
CACCGATAGTCTCTATTTTT

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MKDYQKEFIEFALEKQVLKFGQFTLKSGRTSPYFFNAGLFNSGRDLARLGRFYAAALMDSGIEYDLLFGPAYKGIPIATT
TAVALSNDYDLDIPYCFNRKEAKTHGEGGSLVGSKLQGKIMLVDDVITAGTAIRESMDIIQAHHAQLAGVLIALDRQEKG
RAELSAIQEVERDYGAKVISIVKLSDLITYLEAKPEMQKDLAAVNTYREQYGI

Sequences:

>Translated_213_residues
MKDYQKEFIEFALEKQVLKFGQFTLKSGRTSPYFFNAGLFNSGRDLARLGRFYAAALMDSGIEYDLLFGPAYKGIPIATT
TAVALSNDYDLDIPYCFNRKEAKTHGEGGSLVGSKLQGKIMLVDDVITAGTAIRESMDIIQAHHAQLAGVLIALDRQEKG
RAELSAIQEVERDYGAKVISIVKLSDLITYLEAKPEMQKDLAAVNTYREQYGI
>Mature_213_residues
MKDYQKEFIEFALEKQVLKFGQFTLKSGRTSPYFFNAGLFNSGRDLARLGRFYAAALMDSGIEYDLLFGPAYKGIPIATT
TAVALSNDYDLDIPYCFNRKEAKTHGEGGSLVGSKLQGKIMLVDDVITAGTAIRESMDIIQAHHAQLAGVLIALDRQEKG
RAELSAIQEVERDYGAKVISIVKLSDLITYLEAKPEMQKDLAAVNTYREQYGI

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=179, Percent_Identity=27.9329608938547, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1790073, Length=213, Percent_Identity=79.3427230046948, Blast_Score=355, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI17508631, Length=163, Percent_Identity=28.8343558282209, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17554964, Length=189, Percent_Identity=25.9259259259259, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323530, Length=220, Percent_Identity=49.5454545454545, Blast_Score=207, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6323927, Length=223, Percent_Identity=44.3946188340807, Blast_Score=189, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 23618; Mature: 23618

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00136 SUBTILASE_ASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDYQKEFIEFALEKQVLKFGQFTLKSGRTSPYFFNAGLFNSGRDLARLGRFYAAALMDS
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHC
GIEYDLLFGPAYKGIPIATTTAVALSNDYDLDIPYCFNRKEAKTHGEGGSLVGSKLQGKI
CCCEEEEECCCCCCCCEEHHEEEEECCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCE
MLVDDVITAGTAIRESMDIIQAHHAQLAGVLIALDRQEKGRAELSAIQEVERDYGAKVIS
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHCCHHHH
IVKLSDLITYLEAKPEMQKDLAAVNTYREQYGI
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKDYQKEFIEFALEKQVLKFGQFTLKSGRTSPYFFNAGLFNSGRDLARLGRFYAAALMDS
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHC
GIEYDLLFGPAYKGIPIATTTAVALSNDYDLDIPYCFNRKEAKTHGEGGSLVGSKLQGKI
CCCEEEEECCCCCCCCEEHHEEEEECCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCE
MLVDDVITAGTAIRESMDIIQAHHAQLAGVLIALDRQEKGRAELSAIQEVERDYGAKVIS
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHCCHHHH
IVKLSDLITYLEAKPEMQKDLAAVNTYREQYGI
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]