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The map label for this gene is rph

Identifier: 119947081

GI number: 119947081

Start: 4303114

End: 4303833

Strand: Reverse

Name: rph

Synonym: Ping_3479

Alternate gene names: 119947081

Gene position: 4303833-4303114 (Counterclockwise)

Preceding gene: 119947082

Following gene: 119947080

Centisome position: 94.39

GC content: 47.36

Gene sequence:

>720_bases
ATGCGCCCTAGTAACCGTACTCCCGAACAAAACCGCGAAATCAAAATAACCCGTCATTATACTGACTATGCTGAAGGCTC
GGTATTAATTGAAATGGGTAATACTAAAGTACTTTGTAATGCCTCTGTTTCCGGTTCTGTGCCGCGTTTTTTAAAAGGTA
AAGGCAAAGGTTGGGTGACCGCTGAATACGCTATGTTGCCGCGTGCGACACATATCCGTAATATGCGTGAAGCCGCAAAA
GGCAAGCAGGGCGGACGGACGCTTGAAATCCAGCGCCTTATTGCACGTTCACTGCGCGCCGCTGTTGATCTCAATGCGCT
GGGTGAAAATATGATCACCGTTGATTGTGATGTGATTCAAGCCGATGGTGGCACCCGCACCGCTGCCATTACAGGCGGCT
GTATTGCCTTAGCTGACGCACTTAATTGGATGGTAAAGAAGGGTAAACTGAAAAAAAGTCCGCTGAAGCAGATGATTGCT
GCGGTTTCTGTCGGTATCTACCAAGGCACACCGGTATGTGATTTAGATTATGCGGAAGATTCCAACGCGGAAACAGACAC
GAATGTGGTGATGACCGAAAGCGGTGGTTTAATTGAGATTCAAGCAACAGCTGAAGATGGCGCATTTAGTCATGAACAGC
TGCTTAGCATGTTAAGTCTTGCCAAGGGCGGAATTGAAGCTCTTGTTGTGGAACAGAAAAAAGCACTCAACGCGGAATAA

Upstream 100 bases:

>100_bases
TTACCTGAAGCTGCAGCTTTAAGTAATGTGGGTATAACGTGACCTGTATTAACTTTACTCTTATAATAAACCTTCATTTT
TAAAGAAGGCTTATTAATTT

Downstream 100 bases:

>100_bases
AAAAAATGAAAGATTATCAAAAAGAATTTATTGAATTTGCCTTAGAAAAACAGGTTTTAAAGTTTGGCCAGTTTACCCTG
AAATCGGGCCGTACCAGCCC

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MRPSNRTPEQNREIKITRHYTDYAEGSVLIEMGNTKVLCNASVSGSVPRFLKGKGKGWVTAEYAMLPRATHIRNMREAAK
GKQGGRTLEIQRLIARSLRAAVDLNALGENMITVDCDVIQADGGTRTAAITGGCIALADALNWMVKKGKLKKSPLKQMIA
AVSVGIYQGTPVCDLDYAEDSNAETDTNVVMTESGGLIEIQATAEDGAFSHEQLLSMLSLAKGGIEALVVEQKKALNAE

Sequences:

>Translated_239_residues
MRPSNRTPEQNREIKITRHYTDYAEGSVLIEMGNTKVLCNASVSGSVPRFLKGKGKGWVTAEYAMLPRATHIRNMREAAK
GKQGGRTLEIQRLIARSLRAAVDLNALGENMITVDCDVIQADGGTRTAAITGGCIALADALNWMVKKGKLKKSPLKQMIA
AVSVGIYQGTPVCDLDYAEDSNAETDTNVVMTESGGLIEIQATAEDGAFSHEQLLSMLSLAKGGIEALVVEQKKALNAE
>Mature_239_residues
MRPSNRTPEQNREIKITRHYTDYAEGSVLIEMGNTKVLCNASVSGSVPRFLKGKGKGWVTAEYAMLPRATHIRNMREAAK
GKQGGRTLEIQRLIARSLRAAVDLNALGENMITVDCDVIQADGGTRTAAITGGCIALADALNWMVKKGKLKKSPLKQMIA
AVSVGIYQGTPVCDLDYAEDSNAETDTNVVMTESGGLIEIQATAEDGAFSHEQLLSMLSLAKGGIEALVVEQKKALNAE

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=211, Percent_Identity=68.7203791469194, Blast_Score=309, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI71981632, Length=184, Percent_Identity=27.7173913043478, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_PSYIN (A1T097)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_944761.1
- ProteinModelPortal:   A1T097
- SMR:   A1T097
- STRING:   A1T097
- GeneID:   4625552
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_3479
- eggNOG:   COG0689
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- PhylomeDB:   A1T097
- BioCyc:   PING357804:PING_3479-MONOMER
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 25694; Mature: 25694

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPSNRTPEQNREIKITRHYTDYAEGSVLIEMGNTKVLCNASVSGSVPRFLKGKGKGWVT
CCCCCCCCCCCCCEEEEEEECCCCCCEEEEEECCEEEEEECCCCCCCCHHHCCCCCCEEE
AEYAMLPRATHIRNMREAAKGKQGGRTLEIQRLIARSLRAAVDLNALGENMITVDCDVIQ
EHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEE
ADGGTRTAAITGGCIALADALNWMVKKGKLKKSPLKQMIAAVSVGIYQGTPVCDLDYAED
CCCCCEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCC
SNAETDTNVVMTESGGLIEIQATAEDGAFSHEQLLSMLSLAKGGIEALVVEQKKALNAE
CCCCCCCCEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEEHHHHCCCC
>Mature Secondary Structure
MRPSNRTPEQNREIKITRHYTDYAEGSVLIEMGNTKVLCNASVSGSVPRFLKGKGKGWVT
CCCCCCCCCCCCCEEEEEEECCCCCCEEEEEECCEEEEEECCCCCCCCHHHCCCCCCEEE
AEYAMLPRATHIRNMREAAKGKQGGRTLEIQRLIARSLRAAVDLNALGENMITVDCDVIQ
EHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEE
ADGGTRTAAITGGCIALADALNWMVKKGKLKKSPLKQMIAAVSVGIYQGTPVCDLDYAED
CCCCCEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCC
SNAETDTNVVMTESGGLIEIQATAEDGAFSHEQLLSMLSLAKGGIEALVVEQKKALNAE
CCCCCCCCEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEEHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA