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The map label for this gene is lpdA [H]

Identifier: 119947073

GI number: 119947073

Start: 4290994

End: 4292445

Strand: Reverse

Name: lpdA [H]

Synonym: Ping_3471

Alternate gene names: 119947073

Gene position: 4292445-4290994 (Counterclockwise)

Preceding gene: 119947074

Following gene: 119947072

Centisome position: 94.14

GC content: 45.66

Gene sequence:

>1452_bases
ATGAAAAAGTTAAAAGTAGAAGTTGCAGTTATTGGTGGTGGTACAGCGGGACTTGCTGCATACCGGTCTGCTAAAGAAAA
AACCGATTCAGTCATATTAATCGAAGGCGCACAATTTGGTACCACCTGTGCGCGTGTTGGTTGTATGCCCAGTAAATTGT
TAATTGCCGCGGCAGAAGCGGCGCATAGTGCTTCGCATACCGAACCCTTTGGTGTTTTTATTGATGGAAAAATACGAGTT
GACGGCAAAAAAGTAATGCAGCGGATCCGTGATGAGCGGGATCGTTTTGTGAGTTTTGTTGTTGAAGCGGTTGATAGCCT
GCCCGCGCCGGATAAAGTAATAGGTAACGCTAAATTTATCAGCACAACGGAGCTGCAAATTGGCGATCATACCATTGTAA
CAGCCGATAGAATGGTTATAGCAACGGGCTCCCGTCCAAGTTATCCTGGCGTTTGGAATGAACTTGGCGACAAGTTAATT
ATTAATGATGATCTGTTTAATTGGACTGATTTACCAAAATCAATCGCTGTTTTTGGTCCTGGTGTGATTGGTTTGGAGCT
GGGTCAATCATTACATCGTCTCGGTGTTGAAGTTCTAATGTTTGGCATGGGCGGCCAGGTAGGCCCGTTTACCGATCCAC
AGGTAATGGCTTATGCCCAAAAAACATTTTCTGATGAATTCTATTTAGATGCTGATGCCAATGTTAAAAATATGGTGCGC
AAAGGTGAAAATGTTGAAATCACCTACTTAGATAAACAGGGTATCGAGCAGGTCACTGAAGTTGAATATGTTTTAGCAGC
AACGGGGCGCCGTGCCAATGTAGATAACCTGGGGCTTGAAAATATTGAGATTGAAAAAGATGGACGTGGTGTGCCGATTG
CCGATCGCTTTACTATGCAGACATCGGTACCCTCTATTTTTATTGCAGGGGACGCCAGCAACCAACTCCCGCTGCTTCAT
GAAGCGGCAGATCAAGGTAAAATAGCAGGCAGTAACGCCGCTGTTTATCCCAATATTCGCGTGGGATTACGCCGCTCATC
GATTGCTGCGGTATTTACCGAACCTCAGCTGGCAATGGTGGGCTTAACCCATCAACAAGTCACTGAGAAATACACCGGCT
GCGACTGTTTTGAAACGGGAAGTGTTTCTTTTGAAAGTCAGGGGCGTAGCCGTGTGATCCTTAAAAACCAAGGTTTATTA
AATATTTATGGTGCGCATGGTACCGGGCGTTTTTTAGGTGCAGAAATGATTGGCCCGCAGGCTGAACATCTGGCACATCT
GTTAGCCTGGGCGCATCAGAACAGAATGACTATTTCCCAGATGCTGGATATGCCTTTTTATCATCCGGTAATCGAAGAGG
GGGTGCGTACCGCACTACGCGATTTGAATGCTAAACTGAGACTTGGCTCGGAAATGCTTAATGAATGTATGGAATGCGGA
CCCGGCTGTTAA

Upstream 100 bases:

>100_bases
CATTTAAGCTCATATAAAAAGCGCACTTTCAACAGGAAGCTGCGCTTTTTTTTTACCAAAATTTCACCAGATTGTGTAAT
CAATTTAAGCTAGGAGTTTT

Downstream 100 bases:

>100_bases
TTAGTTAAGCTTCAGTCACATTGTTTGCTTAAATATTGGTGCTTCAACTGTTTTTTTCAACCTGTTAAATACTTTCAAAA
ATTTTAATAGGTTGTTATTA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MKKLKVEVAVIGGGTAGLAAYRSAKEKTDSVILIEGAQFGTTCARVGCMPSKLLIAAAEAAHSASHTEPFGVFIDGKIRV
DGKKVMQRIRDERDRFVSFVVEAVDSLPAPDKVIGNAKFISTTELQIGDHTIVTADRMVIATGSRPSYPGVWNELGDKLI
INDDLFNWTDLPKSIAVFGPGVIGLELGQSLHRLGVEVLMFGMGGQVGPFTDPQVMAYAQKTFSDEFYLDADANVKNMVR
KGENVEITYLDKQGIEQVTEVEYVLAATGRRANVDNLGLENIEIEKDGRGVPIADRFTMQTSVPSIFIAGDASNQLPLLH
EAADQGKIAGSNAAVYPNIRVGLRRSSIAAVFTEPQLAMVGLTHQQVTEKYTGCDCFETGSVSFESQGRSRVILKNQGLL
NIYGAHGTGRFLGAEMIGPQAEHLAHLLAWAHQNRMTISQMLDMPFYHPVIEEGVRTALRDLNAKLRLGSEMLNECMECG
PGC

Sequences:

>Translated_483_residues
MKKLKVEVAVIGGGTAGLAAYRSAKEKTDSVILIEGAQFGTTCARVGCMPSKLLIAAAEAAHSASHTEPFGVFIDGKIRV
DGKKVMQRIRDERDRFVSFVVEAVDSLPAPDKVIGNAKFISTTELQIGDHTIVTADRMVIATGSRPSYPGVWNELGDKLI
INDDLFNWTDLPKSIAVFGPGVIGLELGQSLHRLGVEVLMFGMGGQVGPFTDPQVMAYAQKTFSDEFYLDADANVKNMVR
KGENVEITYLDKQGIEQVTEVEYVLAATGRRANVDNLGLENIEIEKDGRGVPIADRFTMQTSVPSIFIAGDASNQLPLLH
EAADQGKIAGSNAAVYPNIRVGLRRSSIAAVFTEPQLAMVGLTHQQVTEKYTGCDCFETGSVSFESQGRSRVILKNQGLL
NIYGAHGTGRFLGAEMIGPQAEHLAHLLAWAHQNRMTISQMLDMPFYHPVIEEGVRTALRDLNAKLRLGSEMLNECMECG
PGC
>Mature_483_residues
MKKLKVEVAVIGGGTAGLAAYRSAKEKTDSVILIEGAQFGTTCARVGCMPSKLLIAAAEAAHSASHTEPFGVFIDGKIRV
DGKKVMQRIRDERDRFVSFVVEAVDSLPAPDKVIGNAKFISTTELQIGDHTIVTADRMVIATGSRPSYPGVWNELGDKLI
INDDLFNWTDLPKSIAVFGPGVIGLELGQSLHRLGVEVLMFGMGGQVGPFTDPQVMAYAQKTFSDEFYLDADANVKNMVR
KGENVEITYLDKQGIEQVTEVEYVLAATGRRANVDNLGLENIEIEKDGRGVPIADRFTMQTSVPSIFIAGDASNQLPLLH
EAADQGKIAGSNAAVYPNIRVGLRRSSIAAVFTEPQLAMVGLTHQQVTEKYTGCDCFETGSVSFESQGRSRVILKNQGLL
NIYGAHGTGRFLGAEMIGPQAEHLAHLLAWAHQNRMTISQMLDMPFYHPVIEEGVRTALRDLNAKLRLGSEMLNECMECG
PGC

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=473, Percent_Identity=27.061310782241, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI50301238, Length=460, Percent_Identity=24.7826086956522, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI291045266, Length=451, Percent_Identity=25.0554323725055, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI33519430, Length=461, Percent_Identity=24.0780911062907, Blast_Score=86, Evalue=9e-17,
Organism=Homo sapiens, GI33519428, Length=461, Percent_Identity=24.0780911062907, Blast_Score=86, Evalue=9e-17,
Organism=Homo sapiens, GI33519426, Length=461, Percent_Identity=24.0780911062907, Blast_Score=86, Evalue=9e-17,
Organism=Homo sapiens, GI148277071, Length=463, Percent_Identity=24.4060475161987, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI148277065, Length=462, Percent_Identity=24.025974025974, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI291045268, Length=315, Percent_Identity=27.6190476190476, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1786307, Length=471, Percent_Identity=26.5392781316348, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI87082354, Length=476, Percent_Identity=25.4201680672269, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1789915, Length=359, Percent_Identity=27.5766016713092, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI87081717, Length=464, Percent_Identity=22.8448275862069, Blast_Score=83, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI32565766, Length=467, Percent_Identity=28.051391862955, Blast_Score=152, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17557007, Length=472, Percent_Identity=25.2118644067797, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71983419, Length=368, Percent_Identity=25.2717391304348, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI71983429, Length=368, Percent_Identity=25.2717391304348, Blast_Score=92, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6321091, Length=482, Percent_Identity=27.8008298755187, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6325166, Length=387, Percent_Identity=27.390180878553, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21358499, Length=473, Percent_Identity=27.061310782241, Blast_Score=142, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24640553, Length=492, Percent_Identity=27.0325203252033, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24640549, Length=490, Percent_Identity=26.734693877551, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24640551, Length=495, Percent_Identity=27.8787878787879, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI17737741, Length=498, Percent_Identity=24.2971887550201, Blast_Score=86, Evalue=5e-17,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 52388; Mature: 52388

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLKVEVAVIGGGTAGLAAYRSAKEKTDSVILIEGAQFGTTCARVGCMPSKLLIAAAEA
CCCEEEEEEEEECCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHCCCCHHHHHHHHHH
AHSASHTEPFGVFIDGKIRVDGKKVMQRIRDERDRFVSFVVEAVDSLPAPDKVIGNAKFI
HHCCCCCCCEEEEEECEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEE
STTELQIGDHTIVTADRMVIATGSRPSYPGVWNELGDKLIINDDLFNWTDLPKSIAVFGP
EEEEEEECCEEEEEECEEEEEECCCCCCCCHHHHHCCEEEEECCCCCCCCCCCHHHHHCC
GVIGLELGQSLHRLGVEVLMFGMGGQVGPFTDPQVMAYAQKTFSDEFYLDADANVKNMVR
CEEHHHHHHHHHHCCHHEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCHHHHHH
KGENVEITYLDKQGIEQVTEVEYVLAATGRRANVDNLGLENIEIEKDGRGVPIADRFTMQ
CCCCEEEEEECHHHHHHHHHHHHHEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEE
TSVPSIFIAGDASNQLPLLHEAADQGKIAGSNAAVYPNIRVGLRRSSIAAVFTEPQLAMV
CCCCEEEEECCCCCCCCHHHHHCCCCCCCCCCCEECCCEEEEEECCCEEEEEECCCEEEE
GLTHQQVTEKYTGCDCFETGSVSFESQGRSRVILKNQGLLNIYGAHGTGRFLGAEMIGPQ
ECCHHHHHHHHCCCCCEECCCEEECCCCCEEEEEECCCEEEEEECCCCCCEECHHHCCCC
AEHLAHLLAWAHQNRMTISQMLDMPFYHPVIEEGVRTALRDLNAKLRLGSEMLNECMECG
HHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHCC
PGC
CCC
>Mature Secondary Structure
MKKLKVEVAVIGGGTAGLAAYRSAKEKTDSVILIEGAQFGTTCARVGCMPSKLLIAAAEA
CCCEEEEEEEEECCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHCCCCHHHHHHHHHH
AHSASHTEPFGVFIDGKIRVDGKKVMQRIRDERDRFVSFVVEAVDSLPAPDKVIGNAKFI
HHCCCCCCCEEEEEECEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEE
STTELQIGDHTIVTADRMVIATGSRPSYPGVWNELGDKLIINDDLFNWTDLPKSIAVFGP
EEEEEEECCEEEEEECEEEEEECCCCCCCCHHHHHCCEEEEECCCCCCCCCCCHHHHHCC
GVIGLELGQSLHRLGVEVLMFGMGGQVGPFTDPQVMAYAQKTFSDEFYLDADANVKNMVR
CEEHHHHHHHHHHCCHHEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCHHHHHH
KGENVEITYLDKQGIEQVTEVEYVLAATGRRANVDNLGLENIEIEKDGRGVPIADRFTMQ
CCCCEEEEEECHHHHHHHHHHHHHEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEE
TSVPSIFIAGDASNQLPLLHEAADQGKIAGSNAAVYPNIRVGLRRSSIAAVFTEPQLAMV
CCCCEEEEECCCCCCCCHHHHHCCCCCCCCCCCEECCCEEEEEECCCEEEEEECCCEEEE
GLTHQQVTEKYTGCDCFETGSVSFESQGRSRVILKNQGLLNIYGAHGTGRFLGAEMIGPQ
ECCHHHHHHHHCCCCCEECCCEEECCCCCEEEEEECCCEEEEEECCCCCCEECHHHCCCC
AEHLAHLLAWAHQNRMTISQMLDMPFYHPVIEEGVRTALRDLNAKLRLGSEMLNECMECG
HHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHCC
PGC
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10192388; 10684935; 10871362 [H]