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The map label for this gene is rfbB1 [H]

Identifier: 119947067

GI number: 119947067

Start: 4285636

End: 4286718

Strand: Reverse

Name: rfbB1 [H]

Synonym: Ping_3465

Alternate gene names: 119947067

Gene position: 4286718-4285636 (Counterclockwise)

Preceding gene: 119947072

Following gene: 119947066

Centisome position: 94.02

GC content: 40.35

Gene sequence:

>1083_bases
ATGAAAATTTTAGTGACAGGAGGAGCTGGTTTTATTGGCTCAGCGGTTATCCGCAATATCATTAGCAACACAAAAGATAG
TGTTATCAATGTTGATAAATTGACCTATGCAGGAAACTTAGAATCCCTATCTGAAATCGAAGCGAGTGAACGTTATTCAT
TTGAACAAGTCGATATTTGTAATCGTACCGAGTTAGATGGTGTATTTAAAAAACATCAACCCGATGCTGTGATGCATTTA
GCTGCTGAATCCCACGTTGATCGCTCAATTGATGGCCCCGCTGCGTTTATCGAGACGAATATAGTAGGTACTTACACCCT
GCTCGAAGCGTCTCGTCAATATTGGAACCAGCTAAAAGCATCTCGTAAAGAAGCATTTCGTTTTCATCATATTTCAACGG
ATGAAGTTTATGGTGATCTGGAAGGCACTGATGATCTTTTTACTGAAACGACATCTTATGAGCCATCAAGCCCTTATTCA
GCTTCAAAAGCATCCAGTGACCATTTAGTTCGAGCGTGGCAGCGTACTTACGGGCTGCCAACGTTAATAACGAACTGTTC
AAATAATTATGGTCCTTTCCATTTCCCAGAAAAATTGATACCGCTGATAATTTTAAACGCATTAGATGGAAAACCCCTGC
CAGTTTATGGCAATGGCATGCAAATTCGTGATTGGCTGTATGTTGAAGATCATGCTAGTGCACTTTATACCGTTGTAACT
AAAGGTGAAATTGGTGAGACTTACAATATTGGCGGGCATAATGAAAAAGCAAATATTGAAGTAGTAACAACCATCTGTTC
GTTGCTTGAAGAACTAGTTGCAAATAAGCCTGCAAATGTGACTAAATACGAAGATCTTATTACTTATGTAACAGATCGCC
CAGGGCATGATGTGCGCTACGCAATTGACGCGTCAAAAATTGAACGTGAGCTTGGCTGGGCGCCTGCAGAAACATTTGAA
TCTGGGCTTCGTAAAACAGTCGAGTGGTACCTAAATAATAAAAAATGGTGGAGCCGTGTTCTTGATGGTTCATATAATTT
TGAGCGTCTAGGCACAGCAAGTACAAGTGAGGAGAATAAATGA

Upstream 100 bases:

>100_bases
CATTAATGTTAACTTATTTGTGGTTTATTTATAACTAAAATCAGTTTTGAGGTTAGCCGTACATAAGAATTAAACTCACC
ATTATTTAAAAGAAGATAAA

Downstream 100 bases:

>100_bases
AAGGCATTATCTTAGCTGGTGGCTCGGGAACGCGTTTATACCCGATTACTCGTGGTGTTTCAAAGCAACTATTACCAGTA
TATGACAAACCAATGGTTTA

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKILVTGGAGFIGSAVIRNIISNTKDSVINVDKLTYAGNLESLSEIEASERYSFEQVDICNRTELDGVFKKHQPDAVMHL
AAESHVDRSIDGPAAFIETNIVGTYTLLEASRQYWNQLKASRKEAFRFHHISTDEVYGDLEGTDDLFTETTSYEPSSPYS
ASKASSDHLVRAWQRTYGLPTLITNCSNNYGPFHFPEKLIPLIILNALDGKPLPVYGNGMQIRDWLYVEDHASALYTVVT
KGEIGETYNIGGHNEKANIEVVTTICSLLEELVANKPANVTKYEDLITYVTDRPGHDVRYAIDASKIERELGWAPAETFE
SGLRKTVEWYLNNKKWWSRVLDGSYNFERLGTASTSEENK

Sequences:

>Translated_360_residues
MKILVTGGAGFIGSAVIRNIISNTKDSVINVDKLTYAGNLESLSEIEASERYSFEQVDICNRTELDGVFKKHQPDAVMHL
AAESHVDRSIDGPAAFIETNIVGTYTLLEASRQYWNQLKASRKEAFRFHHISTDEVYGDLEGTDDLFTETTSYEPSSPYS
ASKASSDHLVRAWQRTYGLPTLITNCSNNYGPFHFPEKLIPLIILNALDGKPLPVYGNGMQIRDWLYVEDHASALYTVVT
KGEIGETYNIGGHNEKANIEVVTTICSLLEELVANKPANVTKYEDLITYVTDRPGHDVRYAIDASKIERELGWAPAETFE
SGLRKTVEWYLNNKKWWSRVLDGSYNFERLGTASTSEENK
>Mature_360_residues
MKILVTGGAGFIGSAVIRNIISNTKDSVINVDKLTYAGNLESLSEIEASERYSFEQVDICNRTELDGVFKKHQPDAVMHL
AAESHVDRSIDGPAAFIETNIVGTYTLLEASRQYWNQLKASRKEAFRFHHISTDEVYGDLEGTDDLFTETTSYEPSSPYS
ASKASSDHLVRAWQRTYGLPTLITNCSNNYGPFHFPEKLIPLIILNALDGKPLPVYGNGMQIRDWLYVEDHASALYTVVT
KGEIGETYNIGGHNEKANIEVVTTICSLLEELVANKPANVTKYEDLITYVTDRPGHDVRYAIDASKIERELGWAPAETFE
SGLRKTVEWYLNNKKWWSRVLDGSYNFERLGTASTSEENK

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=340, Percent_Identity=35.8823529411765, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI42516563, Length=334, Percent_Identity=23.9520958083832, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI56237023, Length=369, Percent_Identity=27.10027100271, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI56118217, Length=369, Percent_Identity=27.10027100271, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI189083684, Length=369, Percent_Identity=27.10027100271, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI48994969, Length=350, Percent_Identity=77.1428571428572, Blast_Score=579, Evalue=1e-166,
Organism=Escherichia coli, GI1788353, Length=354, Percent_Identity=72.0338983050847, Blast_Score=535, Evalue=1e-153,
Organism=Escherichia coli, GI1786974, Length=352, Percent_Identity=25.5681818181818, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788366, Length=351, Percent_Identity=23.9316239316239, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788365, Length=346, Percent_Identity=21.9653179190751, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17568069, Length=332, Percent_Identity=37.9518072289157, Blast_Score=212, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI115532424, Length=341, Percent_Identity=33.1378299120235, Blast_Score=176, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17539532, Length=340, Percent_Identity=25.2941176470588, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71982035, Length=368, Percent_Identity=24.7282608695652, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71982038, Length=370, Percent_Identity=24.5945945945946, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17507723, Length=199, Percent_Identity=26.1306532663317, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI133901788, Length=193, Percent_Identity=26.9430051813472, Blast_Score=65, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI133901786, Length=193, Percent_Identity=26.9430051813472, Blast_Score=65, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17539422, Length=193, Percent_Identity=26.9430051813472, Blast_Score=65, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI133901790, Length=193, Percent_Identity=26.9430051813472, Blast_Score=65, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17539424, Length=193, Percent_Identity=26.9430051813472, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=365, Percent_Identity=26.5753424657534, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19923002, Length=372, Percent_Identity=26.8817204301075, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21356223, Length=336, Percent_Identity=23.8095238095238, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 40394; Mature: 40394

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVTGGAGFIGSAVIRNIISNTKDSVINVDKLTYAGNLESLSEIEASERYSFEQVDIC
CEEEEECCCCHHHHHHHHHHHCCCCCCEEEEHHEEECCCHHHHHHHHHHHCCCCHHCCCC
NRTELDGVFKKHQPDAVMHLAAESHVDRSIDGPAAFIETNIVGTYTLLEASRQYWNQLKA
CCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHH
SRKEAFRFHHISTDEVYGDLEGTDDLFTETTSYEPSSPYSASKASSDHLVRAWQRTYGLP
HHHHHHHEECCCHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCH
TLITNCSNNYGPFHFPEKLIPLIILNALDGKPLPVYGNGMQIRDWLYVEDHASALYTVVT
HHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCEEEEEEEEEECCCCEEEEEEE
KGEIGETYNIGGHNEKANIEVVTTICSLLEELVANKPANVTKYEDLITYVTDRPGHDVRY
CCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEE
AIDASKIERELGWAPAETFESGLRKTVEWYLNNKKWWSRVLDGSYNFERLGTASTSEENK
EECHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHCCCCCCCCCCC
>Mature Secondary Structure
MKILVTGGAGFIGSAVIRNIISNTKDSVINVDKLTYAGNLESLSEIEASERYSFEQVDIC
CEEEEECCCCHHHHHHHHHHHCCCCCCEEEEHHEEECCCHHHHHHHHHHHCCCCHHCCCC
NRTELDGVFKKHQPDAVMHLAAESHVDRSIDGPAAFIETNIVGTYTLLEASRQYWNQLKA
CCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHH
SRKEAFRFHHISTDEVYGDLEGTDDLFTETTSYEPSSPYSASKASSDHLVRAWQRTYGLP
HHHHHHHEECCCHHHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCH
TLITNCSNNYGPFHFPEKLIPLIILNALDGKPLPVYGNGMQIRDWLYVEDHASALYTVVT
HHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCEEEEEEEEEECCCCEEEEEEE
KGEIGETYNIGGHNEKANIEVVTTICSLLEELVANKPANVTKYEDLITYVTDRPGHDVRY
CCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCEEE
AIDASKIERELGWAPAETFESGLRKTVEWYLNNKKWWSRVLDGSYNFERLGTASTSEENK
EECHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8022265; 10710307 [H]