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The map label for this gene is ansA [H]

Identifier: 119946999

GI number: 119946999

Start: 4195300

End: 4196310

Strand: Reverse

Name: ansA [H]

Synonym: Ping_3394

Alternate gene names: 119946999

Gene position: 4196310-4195300 (Counterclockwise)

Preceding gene: 119947001

Following gene: 119946998

Centisome position: 92.03

GC content: 41.64

Gene sequence:

>1011_bases
ATGGCACATAAAAAAAGAATTTATATCGCATACACAGGCGGCACCATTGGCATGAAACCCTCAAAAGAAGGCTTCATTCC
GGTAGCAGGTTATCTTTCAAAAACCATTGCTAATATGCCGGAGTTTTATCATAGCCAAATGCCTGATTTTGAGATTCATG
AATATGATCCGTTGATTGATTCAGCCAATGTATCCCCTTCTGTCTGGCAGGATCTTGCGCAGGATATTCAAGCGCGTTAC
CACGACTTTGATGGTTTTATTATTCTCCATGGTACCGATACCATGGCTTATACCGCTTCGGCATTATCCTTTGTATTTGA
AAATTTATCCAAGCCAGTGATTGTGACGGGATCACAAATTCCATTGTCACAATTACGTTCCGATGCGCGCGCTAATTTAT
TAAACTCTTTGTATATTGCCGCGCATCATCCTATTAATGAAGTCTGTGTTTTTTTTAATAATCAATTGTTTCGCGGTAAC
CGTGCAAGCAAACAACATGCCGATGGATTTAGTGCTTTTGTTTCTCCTAACCACCCAGTTTTGCTGGAAGCAGGGATTGA
AATCAAGAATATTGCCGGTATTACCCAAAAAGCGGTTAATACCCGTTTACGCATTACTGATATTCGTGCGCATTCGATTG
CCATTCTGCACCTTTTCCCCGGCTTAGATTGGAATATTCTGGATACTTTATTAAAAAGCCCATTAAAGGCACTTGTACTG
TTAACCTATGGGGTGGGAAATGCGCAGCAAGATGAGCAATTATTAGCGATTCTAAAAGAAGCCACGGATCGTGGTATTTT
AATTGTTAATTGCAGTCAGTGTTTAAAAGGCAGCGTGAATATGGAGGGTTATGCCACCGGGCATGCACTGAGTGAGGCGG
GCGTTATCAGCGGGCTGGATATGACCACAGAAACCGTTATTGCCAAGCTATATTATCTGCTCAGTCAAGAGTTACCAACG
AAAGTGATTCGTGAATTGTTAGGGACTAATCTGCGCGGTGAATTAACTTAA

Upstream 100 bases:

>100_bases
TAGAATTAGACAGATAAAAACAGTCTGAATCTAATCGCTAAAAATGGTTTAAATGAGAACCTAAATGAAAACCTAAATGA
AAACCTAAATGAGAAGTTAA

Downstream 100 bases:

>100_bases
TTTATTGCATTTTGTACATAAAAAAACCAGCAATACTGCTTAATGCCGATCAGTTAAGAAAAATATATTGATTTTTAGCT
GAACGAGATCAAAATCCGAT

Product: cytoplasmic asparaginase I

Products: NA

Alternate protein names: L-asparaginase I; L-ASNase I; L-asparagine amidohydrolase I [H]

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MAHKKRIYIAYTGGTIGMKPSKEGFIPVAGYLSKTIANMPEFYHSQMPDFEIHEYDPLIDSANVSPSVWQDLAQDIQARY
HDFDGFIILHGTDTMAYTASALSFVFENLSKPVIVTGSQIPLSQLRSDARANLLNSLYIAAHHPINEVCVFFNNQLFRGN
RASKQHADGFSAFVSPNHPVLLEAGIEIKNIAGITQKAVNTRLRITDIRAHSIAILHLFPGLDWNILDTLLKSPLKALVL
LTYGVGNAQQDEQLLAILKEATDRGILIVNCSQCLKGSVNMEGYATGHALSEAGVISGLDMTTETVIAKLYYLLSQELPT
KVIRELLGTNLRGELT

Sequences:

>Translated_336_residues
MAHKKRIYIAYTGGTIGMKPSKEGFIPVAGYLSKTIANMPEFYHSQMPDFEIHEYDPLIDSANVSPSVWQDLAQDIQARY
HDFDGFIILHGTDTMAYTASALSFVFENLSKPVIVTGSQIPLSQLRSDARANLLNSLYIAAHHPINEVCVFFNNQLFRGN
RASKQHADGFSAFVSPNHPVLLEAGIEIKNIAGITQKAVNTRLRITDIRAHSIAILHLFPGLDWNILDTLLKSPLKALVL
LTYGVGNAQQDEQLLAILKEATDRGILIVNCSQCLKGSVNMEGYATGHALSEAGVISGLDMTTETVIAKLYYLLSQELPT
KVIRELLGTNLRGELT
>Mature_335_residues
AHKKRIYIAYTGGTIGMKPSKEGFIPVAGYLSKTIANMPEFYHSQMPDFEIHEYDPLIDSANVSPSVWQDLAQDIQARYH
DFDGFIILHGTDTMAYTASALSFVFENLSKPVIVTGSQIPLSQLRSDARANLLNSLYIAAHHPINEVCVFFNNQLFRGNR
ASKQHADGFSAFVSPNHPVLLEAGIEIKNIAGITQKAVNTRLRITDIRAHSIAILHLFPGLDWNILDTLLKSPLKALVLL
TYGVGNAQQDEQLLAILKEATDRGILIVNCSQCLKGSVNMEGYATGHALSEAGVISGLDMTTETVIAKLYYLLSQELPTK
VIRELLGTNLRGELT

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=356, Percent_Identity=40.7303370786517, Blast_Score=248, Evalue=8e-66,
Organism=Escherichia coli, GI1788065, Length=334, Percent_Identity=58.3832335329341, Blast_Score=422, Evalue=1e-119,
Organism=Escherichia coli, GI1789327, Length=234, Percent_Identity=27.3504273504274, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI193207372, Length=362, Percent_Identity=41.7127071823204, Blast_Score=274, Evalue=5e-74,
Organism=Caenorhabditis elegans, GI17558280, Length=362, Percent_Identity=41.7127071823204, Blast_Score=273, Evalue=7e-74,
Organism=Caenorhabditis elegans, GI17558278, Length=362, Percent_Identity=41.7127071823204, Blast_Score=273, Evalue=8e-74,
Organism=Caenorhabditis elegans, GI193207374, Length=362, Percent_Identity=41.7127071823204, Blast_Score=273, Evalue=9e-74,
Organism=Drosophila melanogaster, GI24639609, Length=362, Percent_Identity=36.7403314917127, Blast_Score=243, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24645530, Length=367, Percent_Identity=37.3297002724796, Blast_Score=231, Evalue=4e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 36995; Mature: 36864

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHKKRIYIAYTGGTIGMKPSKEGFIPVAGYLSKTIANMPEFYHSQMPDFEIHEYDPLID
CCCCEEEEEEEECCEECCCCCCCCCEEHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCC
SANVSPSVWQDLAQDIQARYHDFDGFIILHGTDTMAYTASALSFVFENLSKPVIVTGSQI
CCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEECCCC
PLSQLRSDARANLLNSLYIAAHHPINEVCVFFNNQLFRGNRASKQHADGFSAFVSPNHPV
CHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHCCCEECCCCCCCHHCCCCEEEECCCCCE
LLEAGIEIKNIAGITQKAVNTRLRITDIRAHSIAILHLFPGLDWNILDTLLKSPLKALVL
EEECCCCEEHHHHHHHHHHCCEEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHH
LTYGVGNAQQDEQLLAILKEATDRGILIVNCSQCLKGSVNMEGYATGHALSEAGVISGLD
HEECCCCCCHHHHHHHHHHHHCCCCEEEEECHHHHCCCCCCCCEECCCHHHHCCCCCCCC
MTTETVIAKLYYLLSQELPTKVIRELLGTNLRGELT
HHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AHKKRIYIAYTGGTIGMKPSKEGFIPVAGYLSKTIANMPEFYHSQMPDFEIHEYDPLID
CCCEEEEEEEECCEECCCCCCCCCEEHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCC
SANVSPSVWQDLAQDIQARYHDFDGFIILHGTDTMAYTASALSFVFENLSKPVIVTGSQI
CCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEECCCC
PLSQLRSDARANLLNSLYIAAHHPINEVCVFFNNQLFRGNRASKQHADGFSAFVSPNHPV
CHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHCCCEECCCCCCCHHCCCCEEEECCCCCE
LLEAGIEIKNIAGITQKAVNTRLRITDIRAHSIAILHLFPGLDWNILDTLLKSPLKALVL
EEECCCCEEHHHHHHHHHHCCEEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHH
LTYGVGNAQQDEQLLAILKEATDRGILIVNCSQCLKGSVNMEGYATGHALSEAGVISGLD
HEECCCCCCHHHHHHHHHHHHCCCCEEEEECHHHHCCCCCCCCEECCCHHHHCCCCCCCC
MTTETVIAKLYYLLSQELPTKVIRELLGTNLRGELT
HHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]