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The map label for this gene is trmD

Identifier: 119946970

GI number: 119946970

Start: 4157828

End: 4158574

Strand: Reverse

Name: trmD

Synonym: Ping_3365

Alternate gene names: 119946970

Gene position: 4158574-4157828 (Counterclockwise)

Preceding gene: 119946971

Following gene: 119946969

Centisome position: 91.2

GC content: 46.18

Gene sequence:

>747_bases
ATGTGGATAGGGGTTGTAAGCCTCTTCCCGGATATGTTCCGGGCCATTAGCGACAATGGGGTAACAGGTCGAGCGGTAAA
AAATAAGCTGCTTTCTATTCAATGTTGGAATCCTCGTGATTTCACCTTGGATAAACATAGAACGGTTGATGATCGTCCCT
ATGGTGGTGGGCCTGGCATGTTAATGATGGTGAAACCTTTAAGGGATGCCATTTTAGCTGCCAAAAAGGCTGCAAGGGAA
GCAGGGCACGAGGCAAAGGTGATTTATCTTTCTCCTCAAGGTAAGCGTTTAGATCATTGTGGCGCGCAGTTATTATCAGG
CTCTGATGCTTTGATATTAGTGGCTGGGCGTTATGAGGGTGTAGATGAGCGTTTAATAGAAAGCCTGGTTGACGAAGAAT
GGTCAGTTGGTGATTATGTTTTAAGTGGTGGTGAGTTGCCAGCCATGATTTTAATTGATGCTGTGGTGCGTTTTGTACCC
GGTGTGTTAGGTCATGTGTTATCAGCCGAGCAGGACTCCTTCGCGGATGGATTGCTTGATTGCCCCCACTATACGCGACC
TGAGGTGCTAGATGGAAAGCAAGTGCCAAGTGTATTATTAAGCGGTGATCATGAAAAGATTCGTCTTTGGCGATCAAAAC
AAGCACTGCAACGCACGAAACAAAGAAGGCCCGATTTATTAAATAACCTAGCTCTGACTGACGAGCAAGCTAAATTGCTT
GCTGCACTGGAAAGTGACTCCGACTGA

Upstream 100 bases:

>100_bases
CCGTTCATCGACAAGCAAGTAATTTCCAACGTGGATATTACAAGCAAATTAATTCAAGTTAATTGGGAACCTGATTTCTG
AATACCCTAGGAGAAAAGTG

Downstream 100 bases:

>100_bases
CCAGCGATGTTATTGGCTAACTAGGTAATAAAGGTATTAAAATGAGCAATATTATACAAGCAATTGAAAAAGAACAAATG
CGTAGCGATATGCCTAAATT

Product: tRNA (guanine-N1)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MWIGVVSLFPDMFRAISDNGVTGRAVKNKLLSIQCWNPRDFTLDKHRTVDDRPYGGGPGMLMMVKPLRDAILAAKKAARE
AGHEAKVIYLSPQGKRLDHCGAQLLSGSDALILVAGRYEGVDERLIESLVDEEWSVGDYVLSGGELPAMILIDAVVRFVP
GVLGHVLSAEQDSFADGLLDCPHYTRPEVLDGKQVPSVLLSGDHEKIRLWRSKQALQRTKQRRPDLLNNLALTDEQAKLL
AALESDSD

Sequences:

>Translated_248_residues
MWIGVVSLFPDMFRAISDNGVTGRAVKNKLLSIQCWNPRDFTLDKHRTVDDRPYGGGPGMLMMVKPLRDAILAAKKAARE
AGHEAKVIYLSPQGKRLDHCGAQLLSGSDALILVAGRYEGVDERLIESLVDEEWSVGDYVLSGGELPAMILIDAVVRFVP
GVLGHVLSAEQDSFADGLLDCPHYTRPEVLDGKQVPSVLLSGDHEKIRLWRSKQALQRTKQRRPDLLNNLALTDEQAKLL
AALESDSD
>Mature_248_residues
MWIGVVSLFPDMFRAISDNGVTGRAVKNKLLSIQCWNPRDFTLDKHRTVDDRPYGGGPGMLMMVKPLRDAILAAKKAARE
AGHEAKVIYLSPQGKRLDHCGAQLLSGSDALILVAGRYEGVDERLIESLVDEEWSVGDYVLSGGELPAMILIDAVVRFVP
GVLGHVLSAEQDSFADGLLDCPHYTRPEVLDGKQVPSVLLSGDHEKIRLWRSKQALQRTKQRRPDLLNNLALTDEQAKLL
AALESDSD

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=246, Percent_Identity=71.9512195121951, Blast_Score=351, Evalue=2e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_PSYIN (A1SZY6)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_944650.1
- ProteinModelPortal:   A1SZY6
- SMR:   A1SZY6
- STRING:   A1SZY6
- GeneID:   4625710
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_3365
- eggNOG:   COG0336
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- PhylomeDB:   A1SZY6
- BioCyc:   PING357804:PING_3365-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 27308; Mature: 27308

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: BINDING 116-116

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWIGVVSLFPDMFRAISDNGVTGRAVKNKLLSIQCWNPRDFTLDKHRTVDDRPYGGGPGM
CEEEHHHHHHHHHHHHCCCCCCHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
LMMVKPLRDAILAAKKAAREAGHEAKVIYLSPQGKRLDHCGAQLLSGSDALILVAGRYEG
EEEHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCCCC
VDERLIESLVDEEWSVGDYVLSGGELPAMILIDAVVRFVPGVLGHVLSAEQDSFADGLLD
HHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHC
CPHYTRPEVLDGKQVPSVLLSGDHEKIRLWRSKQALQRTKQRRPDLLNNLALTDEQAKLL
CCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCHHHHHH
AALESDSD
HHHCCCCC
>Mature Secondary Structure
MWIGVVSLFPDMFRAISDNGVTGRAVKNKLLSIQCWNPRDFTLDKHRTVDDRPYGGGPGM
CEEEHHHHHHHHHHHHCCCCCCHHHHHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
LMMVKPLRDAILAAKKAAREAGHEAKVIYLSPQGKRLDHCGAQLLSGSDALILVAGRYEG
EEEHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCCCC
VDERLIESLVDEEWSVGDYVLSGGELPAMILIDAVVRFVPGVLGHVLSAEQDSFADGLLD
HHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHC
CPHYTRPEVLDGKQVPSVLLSGDHEKIRLWRSKQALQRTKQRRPDLLNNLALTDEQAKLL
CCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCHHHHHH
AALESDSD
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA