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The map label for this gene is tapD [H]

Identifier: 119946931

GI number: 119946931

Start: 4106700

End: 4107572

Strand: Reverse

Name: tapD [H]

Synonym: Ping_3325

Alternate gene names: 119946931

Gene position: 4107572-4106700 (Counterclockwise)

Preceding gene: 119946932

Following gene: 119946930

Centisome position: 90.09

GC content: 41.12

Gene sequence:

>873_bases
ATGAATGATATTGTCATCTTGTTAGAGTATTCCCCTCCATACCTTTACGGGATAGTCACTATTCTGGGCTTGTTGATAGG
TTCATTTTTAAATGTTGTTATCTATCGTTTGCCTGTGATGATGGAGCGGGAATGGAAAGCGGAATGTGCGGACTCTTTTC
CCGATGTCAATATCAATATTGAGCCAGATACCTTTAATCTTGTCTTACCTCATTCGCGTTGTCCAAGCTGTCATCATTTG
ATAGGTCCAACGGAAAATATCCCGGTGATCAGCTGGTTGTTACAAAAGGGACGCTGTAAGCATTGTAGCTGTAAAATCAG
CAAACGTTATCCAAGTATTGAATTATTAACCGCTATGCTGTCCTTGGCTGTTGCTTATCTGGTACCGTTTGGCTGGCCAT
TATTATTTGCTTTAGTCTTTACCTGGGTATTAATCAGCCTGACTTTTATTGATTTTGATACGATGTTATTACCCGATAAC
CTGACGCTGCCGTTATTGTGGTTAGGTCTATTAATCAATATTTCCGGCACTTTTACCGATATAAGCAGTGCGGTATTAGG
CGCCATCTTTGGTTATTTAACACTTTGGTCTATCTATTGGGGATTTAAAATTGTCACCGGCAAAGAGGGAATGGGATATG
GTGATTTTAAATTATTGGCGGCTTTAGGGGCGTGGTTCGGCTGGCAGGCATTACCGCTTATTATTTTACTTTCTTCTTTT
TCCGGGGCAATTATCGGTATTGCCATTATGTTAGCCTCCAAGGATAAACAGAGTCGGCCAATGCCCTTTGGTCCCTATCT
TGCCGTTGCGGGGTGGTTATTTCTGGTTTACGGGGATAGGTTAACTGAAATCTATTATTCACTGGTTTTATAA

Upstream 100 bases:

>100_bases
TAGCCCCGCTTCTGCGGCAAAAGAAATCGCCTTCTTTTTTAGCGATGATGAAATATGCCCACCAGCAGCAGATTAAAATG
AGTGAAAGCTGGGTAATTTT

Downstream 100 bases:

>100_bases
TGTCAAATGACAAGACATTGACGGCGCAGCAACACAATGATCAAAAATGGATGGCCTACGCGCTCTCTTTAGCAGATAAA
GCCGAATCGTTCGGTGAGAT

Product: prepilin peptidase

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MNDIVILLEYSPPYLYGIVTILGLLIGSFLNVVIYRLPVMMEREWKAECADSFPDVNINIEPDTFNLVLPHSRCPSCHHL
IGPTENIPVISWLLQKGRCKHCSCKISKRYPSIELLTAMLSLAVAYLVPFGWPLLFALVFTWVLISLTFIDFDTMLLPDN
LTLPLLWLGLLINISGTFTDISSAVLGAIFGYLTLWSIYWGFKIVTGKEGMGYGDFKLLAALGAWFGWQALPLIILLSSF
SGAIIGIAIMLASKDKQSRPMPFGPYLAVAGWLFLVYGDRLTEIYYSLVL

Sequences:

>Translated_290_residues
MNDIVILLEYSPPYLYGIVTILGLLIGSFLNVVIYRLPVMMEREWKAECADSFPDVNINIEPDTFNLVLPHSRCPSCHHL
IGPTENIPVISWLLQKGRCKHCSCKISKRYPSIELLTAMLSLAVAYLVPFGWPLLFALVFTWVLISLTFIDFDTMLLPDN
LTLPLLWLGLLINISGTFTDISSAVLGAIFGYLTLWSIYWGFKIVTGKEGMGYGDFKLLAALGAWFGWQALPLIILLSSF
SGAIIGIAIMLASKDKQSRPMPFGPYLAVAGWLFLVYGDRLTEIYYSLVL
>Mature_290_residues
MNDIVILLEYSPPYLYGIVTILGLLIGSFLNVVIYRLPVMMEREWKAECADSFPDVNINIEPDTFNLVLPHSRCPSCHHL
IGPTENIPVISWLLQKGRCKHCSCKISKRYPSIELLTAMLSLAVAYLVPFGWPLLFALVFTWVLISLTFIDFDTMLLPDN
LTLPLLWLGLLINISGTFTDISSAVLGAIFGYLTLWSIYWGFKIVTGKEGMGYGDFKLLAALGAWFGWQALPLIILLSSF
SGAIIGIAIMLASKDKQSRPMPFGPYLAVAGWLFLVYGDRLTEIYYSLVL

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue. Processes the tapA pilin precursor during membrane translocation [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=267, Percent_Identity=38.9513108614232, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1789732, Length=135, Percent_Identity=51.1111111111111, Blast_Score=104, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 32427; Mature: 32427

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDIVILLEYSPPYLYGIVTILGLLIGSFLNVVIYRLPVMMEREWKAECADSFPDVNINI
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
EPDTFNLVLPHSRCPSCHHLIGPTENIPVISWLLQKGRCKHCSCKISKRYPSIELLTAML
CCCEEEEEECCCCCCCCHHCCCCCCCCHHHHHHHHCCCCCCCCEEHHCCCCCHHHHHHHH
SLAVAYLVPFGWPLLFALVFTWVLISLTFIDFDTMLLPDNLTLPLLWLGLLINISGTFTD
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHCCCCHHH
ISSAVLGAIFGYLTLWSIYWGFKIVTGKEGMGYGDFKLLAALGAWFGWQALPLIILLSSF
HHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
SGAIIGIAIMLASKDKQSRPMPFGPYLAVAGWLFLVYGDRLTEIYYSLVL
CCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNDIVILLEYSPPYLYGIVTILGLLIGSFLNVVIYRLPVMMEREWKAECADSFPDVNINI
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
EPDTFNLVLPHSRCPSCHHLIGPTENIPVISWLLQKGRCKHCSCKISKRYPSIELLTAML
CCCEEEEEECCCCCCCCHHCCCCCCCCHHHHHHHHCCCCCCCCEEHHCCCCCHHHHHHHH
SLAVAYLVPFGWPLLFALVFTWVLISLTFIDFDTMLLPDNLTLPLLWLGLLINISGTFTD
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHCCCCHHH
ISSAVLGAIFGYLTLWSIYWGFKIVTGKEGMGYGDFKLLAALGAWFGWQALPLIILLSSF
HHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
SGAIIGIAIMLASKDKQSRPMPFGPYLAVAGWLFLVYGDRLTEIYYSLVL
CCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA