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The map label for this gene is dacB [H]

Identifier: 119946899

GI number: 119946899

Start: 4065027

End: 4066469

Strand: Reverse

Name: dacB [H]

Synonym: Ping_3293

Alternate gene names: 119946899

Gene position: 4066469-4065027 (Counterclockwise)

Preceding gene: 119946903

Following gene: 119946894

Centisome position: 89.18

GC content: 39.71

Gene sequence:

>1443_bases
ATGTTTAAGCGTATTCTGTGTTTTGCTTTATTTCTACTGCCGGTAAATTTACTGGCTGATCAATGGTCAGCACTGCATGC
TTTATTACCTAAAGGGACACAGCTTTCTTATTTGGTATTGGATCCTGCCAATAATCAATTGCTTGCTGAGTCCAATGCCG
AGATCCTGCGTACTCCGGCAAGCTTACAGAAGTTATTAACAGCGACGGCTGCCAAGTTATATCTAGGTGAAAATTTTCGC
TATCAAACAACCCTTGAGGGCAACAAAAGTCACATTAAAAATAATCATTATCAGGGTGATATTCGCTTACGTTTTGTTGG
GGATCCCACCCTGTCGCGTGCAGATATCAAAGAGATGCTAACATCTTTTAAAGCATTGGGTGTCAAGCAGATAAGGGGTG
ATCTTTTAATTAACGCGGCTCATTTTAGCGGCTATCAATGGAGTGATGGTCAGGCCTGGAATGATTTGGGTGTCTGTTAT
ACCTCTCCGTCAAATGCCATTATTGTTAACAGAAATTGCGTATTAGGTAACCTCAGCGTAGTGAGTGCAGATGCCAAAAA
AGCGCGTTTATTTATTCCAAGTTATGAACCCGTTGAGATAAGCAGTAACGTCGATGTGGTCACATCTGCGCAGCGAGATG
AGCAATTTTGCGCCTTAAAAATGACCCGTAATAGTGATAACCGTTACCATTTATGGGGTTGTATGGTGCCGAGGAATCGT
CCTTTTGGTTTGGCTTTTTCGGTCAACGATCCCTTTGCATATGCGAGTCAAATTATTGAATCAGAACTAAAAAATGTCGG
TATTAAGTTGACTGGTAAGGTGAAAATTGAGCAGATAAAAACAGCTAAGAAAGTCTCGCAAGTATTAGTTAAGTATCAAT
CCCCTGAGCTAGGGAAATTATTGGAAATTATGCTCAAAGACTCAGATAATTTAATTGCGGATAGTCTTTTCAAAACACTG
GGTGCTGCCTATTTTCAACAGGACGGCAATTTTCTCAATGGTGCTAAGGCGCTTAAATTAATTCTGCAGGAGAACGGTGT
TGATTTAGAAAATGCTTATCTGGCCGATGGTTCCGGGTTATCTCGTCATAATTTAATGAGTGCTAAATTGTTTATGACTG
TCCTTAAATATGTTTACCAAAATGATGCTAAATTAAACTTGTTAGACAGTTTTTCAATTGCTGGAGTAGACGGCACACTG
CATTACCATAAAGGGGTCGCAGGACCTTTGTTAAAAGGTAATATTGTTGCAAAAACGGGGTCGATGAAAGGTGTGGCAAA
CTTATTGGGTATTGTTAAAACGGCGCGTGGGGAGCGTCTTTTTGCTTTAATTATAAACGGCTATAATTGGCCTGAGACCG
ATTTAACTAAAGCCACAAAATCACCTGTTGTTATTTTTGAACATGCTTTTTTTGAAACAATAATAAAGTCTTCCCGCAAA
TAA

Upstream 100 bases:

>100_bases
TCTGCCTTTTTTAAATGCTTTAATCAAAGTGAAATGTTTTTTTGAACGCTTTGTTATTGTTAGGGGTTATGATGAGACTT
GACAAATAATTGGAGTTTTT

Downstream 100 bases:

>100_bases
ATAAATCAAAAAGAGGTTTATGCTTCTTTTGGATAAATATGAATTTATTGTATTTTAATCGGGAACCATTTTTTAGGTCT
ACCTTGGCTAAATAAAATAA

Product: D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; D-alanyl-D-alanine endopeptidase; DD-endopeptidase; Penicillin-binding protein 4; PBP-4 [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MFKRILCFALFLLPVNLLADQWSALHALLPKGTQLSYLVLDPANNQLLAESNAEILRTPASLQKLLTATAAKLYLGENFR
YQTTLEGNKSHIKNNHYQGDIRLRFVGDPTLSRADIKEMLTSFKALGVKQIRGDLLINAAHFSGYQWSDGQAWNDLGVCY
TSPSNAIIVNRNCVLGNLSVVSADAKKARLFIPSYEPVEISSNVDVVTSAQRDEQFCALKMTRNSDNRYHLWGCMVPRNR
PFGLAFSVNDPFAYASQIIESELKNVGIKLTGKVKIEQIKTAKKVSQVLVKYQSPELGKLLEIMLKDSDNLIADSLFKTL
GAAYFQQDGNFLNGAKALKLILQENGVDLENAYLADGSGLSRHNLMSAKLFMTVLKYVYQNDAKLNLLDSFSIAGVDGTL
HYHKGVAGPLLKGNIVAKTGSMKGVANLLGIVKTARGERLFALIINGYNWPETDLTKATKSPVVIFEHAFFETIIKSSRK

Sequences:

>Translated_480_residues
MFKRILCFALFLLPVNLLADQWSALHALLPKGTQLSYLVLDPANNQLLAESNAEILRTPASLQKLLTATAAKLYLGENFR
YQTTLEGNKSHIKNNHYQGDIRLRFVGDPTLSRADIKEMLTSFKALGVKQIRGDLLINAAHFSGYQWSDGQAWNDLGVCY
TSPSNAIIVNRNCVLGNLSVVSADAKKARLFIPSYEPVEISSNVDVVTSAQRDEQFCALKMTRNSDNRYHLWGCMVPRNR
PFGLAFSVNDPFAYASQIIESELKNVGIKLTGKVKIEQIKTAKKVSQVLVKYQSPELGKLLEIMLKDSDNLIADSLFKTL
GAAYFQQDGNFLNGAKALKLILQENGVDLENAYLADGSGLSRHNLMSAKLFMTVLKYVYQNDAKLNLLDSFSIAGVDGTL
HYHKGVAGPLLKGNIVAKTGSMKGVANLLGIVKTARGERLFALIINGYNWPETDLTKATKSPVVIFEHAFFETIIKSSRK
>Mature_480_residues
MFKRILCFALFLLPVNLLADQWSALHALLPKGTQLSYLVLDPANNQLLAESNAEILRTPASLQKLLTATAAKLYLGENFR
YQTTLEGNKSHIKNNHYQGDIRLRFVGDPTLSRADIKEMLTSFKALGVKQIRGDLLINAAHFSGYQWSDGQAWNDLGVCY
TSPSNAIIVNRNCVLGNLSVVSADAKKARLFIPSYEPVEISSNVDVVTSAQRDEQFCALKMTRNSDNRYHLWGCMVPRNR
PFGLAFSVNDPFAYASQIIESELKNVGIKLTGKVKIEQIKTAKKVSQVLVKYQSPELGKLLEIMLKDSDNLIADSLFKTL
GAAYFQQDGNFLNGAKALKLILQENGVDLENAYLADGSGLSRHNLMSAKLFMTVLKYVYQNDAKLNLLDSFSIAGVDGTL
HYHKGVAGPLLKGNIVAKTGSMKGVANLLGIVKTARGERLFALIINGYNWPETDLTKATKSPVVIFEHAFFETIIKSSRK

Specific function: Not involved in transpeptidation but exclusively catalyzes a DD-carboxypeptidase and DD-endopeptidase reaction [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=452, Percent_Identity=37.6106194690265, Blast_Score=306, Evalue=1e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 53099; Mature: 53099

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKRILCFALFLLPVNLLADQWSALHALLPKGTQLSYLVLDPANNQLLAESNAEILRTPA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEECCCCCEEECCH
SLQKLLTATAAKLYLGENFRYQTTLEGNKSHIKNNHYQGDIRLRFVGDPTLSRADIKEML
HHHHHHHHHHHHHEECCCCEEEEEECCCHHHHCCCCEECEEEEEEECCCCCCHHHHHHHH
TSFKALGVKQIRGDLLINAAHFSGYQWSDGQAWNDLGVCYTSPSNAIIVNRNCVLGNLSV
HHHHHHCHHHCCCCEEEEEECCCCEECCCCCCCCCCEEEEECCCCEEEEECCEEEECEEE
VSADAKKARLFIPSYEPVEISSNVDVVTSAQRDEQFCALKMTRNSDNRYHLWGCMVPRNR
EECCCCCCEEECCCCCCEEECCCCCEEECCCCCCCEEEEEEECCCCCEEEEEEEECCCCC
PFGLAFSVNDPFAYASQIIESELKNVGIKLTGKVKIEQIKTAKKVSQVLVKYQSPELGKL
CCEEEEECCCCHHHHHHHHHHHHHHCCEEEEEEEEHHHHHHHHHHHHHHHHCCCCCHHHH
LEIMLKDSDNLIADSLFKTLGAAYFQQDGNFLNGAKALKLILQENGVDLENAYLADGSGL
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCEEEECCCCC
SRHNLMSAKLFMTVLKYVYQNDAKLNLLDSFSIAGVDGTLHYHKGVAGPLLKGNIVAKTG
CHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEECCCCEEEECCCCCCCCCCCCEEEECC
SMKGVANLLGIVKTARGERLFALIINGYNWPETDLTKATKSPVVIFEHAFFETIIKSSRK
CCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHCCCCCEEEEHHHHHHHHHHHCCC
>Mature Secondary Structure
MFKRILCFALFLLPVNLLADQWSALHALLPKGTQLSYLVLDPANNQLLAESNAEILRTPA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEECCCCCEEECCH
SLQKLLTATAAKLYLGENFRYQTTLEGNKSHIKNNHYQGDIRLRFVGDPTLSRADIKEML
HHHHHHHHHHHHHEECCCCEEEEEECCCHHHHCCCCEECEEEEEEECCCCCCHHHHHHHH
TSFKALGVKQIRGDLLINAAHFSGYQWSDGQAWNDLGVCYTSPSNAIIVNRNCVLGNLSV
HHHHHHCHHHCCCCEEEEEECCCCEECCCCCCCCCCEEEEECCCCEEEEECCEEEECEEE
VSADAKKARLFIPSYEPVEISSNVDVVTSAQRDEQFCALKMTRNSDNRYHLWGCMVPRNR
EECCCCCCEEECCCCCCEEECCCCCEEECCCCCCCEEEEEEECCCCCEEEEEEEECCCCC
PFGLAFSVNDPFAYASQIIESELKNVGIKLTGKVKIEQIKTAKKVSQVLVKYQSPELGKL
CCEEEEECCCCHHHHHHHHHHHHHHCCEEEEEEEEHHHHHHHHHHHHHHHHCCCCCHHHH
LEIMLKDSDNLIADSLFKTLGAAYFQQDGNFLNGAKALKLILQENGVDLENAYLADGSGL
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCEEEECCCCC
SRHNLMSAKLFMTVLKYVYQNDAKLNLLDSFSIAGVDGTLHYHKGVAGPLLKGNIVAKTG
CHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEECCCCEEEECCCCCCCCCCCCEEEECC
SMKGVANLLGIVKTARGERLFALIINGYNWPETDLTKATKSPVVIFEHAFFETIIKSSRK
CCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHCCCCCEEEEHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]