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The map label for this gene is fre [H]

Identifier: 119946794

GI number: 119946794

Start: 3935848

End: 3936543

Strand: Reverse

Name: fre [H]

Synonym: Ping_3187

Alternate gene names: 119946794

Gene position: 3936543-3935848 (Counterclockwise)

Preceding gene: 119946795

Following gene: 119946793

Centisome position: 86.34

GC content: 39.22

Gene sequence:

>696_bases
ATGCAACCTGTTACTTGTAAGATCACTTCCATCGAAAAATTAAATACTTTTCTATACCGCGTATTTTTAAAACCCATTGA
GCCTGTTTCTTTTAAGGCCGGACAATACGTTTCAATTGTGATGGGAGAAAATGATAAACGTTATTTTTCAATAGCGAACG
CACCAACAGGAGATCAAATTGAGCTGCATATAGGGGCCACACCCGAAAACAGTTATGCAATGGAGGTTATTGAAAAGATG
CAGGATGAAGGCTGTGTTGATGCTGAAATTGGCAATGGTGTTGCTTATTTACGTGAAAGCTCGGAGCGGCCGATTATATT
AGTGGCAGGAGGAACGGGTTTCTCATATGTAAAATCTCTGCTGGAACAAATTGTTCATCTGCAATTAGAAAACCCGGTTT
ATTTATACTGGGGAGTGAAAGAATACGCACACTTTTATTTTGAAGATGAAGCCTCAAGTTGGGCAAATGATCATAAAAAT
ATTCACTTTCATCCAGTGATTGAATTGCCTGAAGCGCATTGGCAAGGTCATCGAGGTTATGTCCATCAAGCTGTTTTGAA
TGAATTTGAAGATTTAAGCAGTTTTGATATCTATGTTGTCGGGCGATTTGAGATGGCAAAAATTGCCCGCGATGATTTTG
TCAAACAAGGTGCGTTGATTGAACATATCTACGGAGATGCGTTTGCTTTCCTTTGA

Upstream 100 bases:

>100_bases
AGATGATCTTTGGCAAGACTTAGATATCCTGTCATAATCTTAATGTTATTTTTTTGTTACATTCCTGTTTTTCTGGGACC
TATTCTAAGAAGAGAAATTA

Downstream 100 bases:

>100_bases
TTTATCCTATCACTTTATTGTTACCACTTAGTGTTCGCGGTATCTCATTTTTTTGAGATACCGCATCTCCGTAAGACTTA
ACTGCCTCCCTTATTTAATT

Product: FMN reductase

Products: NAD(P); FMNH2; 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: NAD(P)H:flavin oxidoreductase [H]

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MQPVTCKITSIEKLNTFLYRVFLKPIEPVSFKAGQYVSIVMGENDKRYFSIANAPTGDQIELHIGATPENSYAMEVIEKM
QDEGCVDAEIGNGVAYLRESSERPIILVAGGTGFSYVKSLLEQIVHLQLENPVYLYWGVKEYAHFYFEDEASSWANDHKN
IHFHPVIELPEAHWQGHRGYVHQAVLNEFEDLSSFDIYVVGRFEMAKIARDDFVKQGALIEHIYGDAFAFL

Sequences:

>Translated_231_residues
MQPVTCKITSIEKLNTFLYRVFLKPIEPVSFKAGQYVSIVMGENDKRYFSIANAPTGDQIELHIGATPENSYAMEVIEKM
QDEGCVDAEIGNGVAYLRESSERPIILVAGGTGFSYVKSLLEQIVHLQLENPVYLYWGVKEYAHFYFEDEASSWANDHKN
IHFHPVIELPEAHWQGHRGYVHQAVLNEFEDLSSFDIYVVGRFEMAKIARDDFVKQGALIEHIYGDAFAFL
>Mature_231_residues
MQPVTCKITSIEKLNTFLYRVFLKPIEPVSFKAGQYVSIVMGENDKRYFSIANAPTGDQIELHIGATPENSYAMEVIEKM
QDEGCVDAEIGNGVAYLRESSERPIILVAGGTGFSYVKSLLEQIVHLQLENPVYLYWGVKEYAHFYFEDEASSWANDHKN
IHFHPVIELPEAHWQGHRGYVHQAVLNEFEDLSSFDIYVVGRFEMAKIARDDFVKQGALIEHIYGDAFAFL

Specific function: Involved in bioluminescence. It is a good supplier of reduced flavin mononucleotide (FMNH2) to the bioluminescence reaction. Preferably uses riboflavin as an electron acceptor when NADPH is used as an electron donor [H]

COG id: COG0543

COG function: function code HC; 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367314, Length=233, Percent_Identity=43.3476394849785, Blast_Score=194, Evalue=4e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00175 NAD_binding_1 [H]

EC number: 1.5.1.29 [C]

Molecular weight: Translated: 26318; Mature: 26318

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPVTCKITSIEKLNTFLYRVFLKPIEPVSFKAGQYVSIVMGENDKRYFSIANAPTGDQI
CCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCEE
ELHIGATPENSYAMEVIEKMQDEGCVDAEIGNGVAYLRESSERPIILVAGGTGFSYVKSL
EEEEECCCCCHHHHHHHHHHHHCCCEEEECCCCEEEEECCCCCCEEEEECCCCHHHHHHH
LEQIVHLQLENPVYLYWGVKEYAHFYFEDEASSWANDHKNIHFHPVIELPEAHWQGHRGY
HHHHHHEEECCCEEEEECHHHHEEEEECCCCHHHHCCCCCEEEEEEEECCHHHCCCCCCH
VHQAVLNEFEDLSSFDIYVVGRFEMAKIARDDFVKQGALIEHIYGDAFAFL
HHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MQPVTCKITSIEKLNTFLYRVFLKPIEPVSFKAGQYVSIVMGENDKRYFSIANAPTGDQI
CCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCEE
ELHIGATPENSYAMEVIEKMQDEGCVDAEIGNGVAYLRESSERPIILVAGGTGFSYVKSL
EEEEECCCCCHHHHHHHHHHHHCCCEEEECCCCEEEEECCCCCCEEEEECCCCHHHHHHH
LEQIVHLQLENPVYLYWGVKEYAHFYFEDEASSWANDHKNIHFHPVIELPEAHWQGHRGY
HHHHHHEEECCCEEEEECHHHHEEEEECCCCHHHHCCCCCEEEEEEEECCHHHCCCCCCH
VHQAVLNEFEDLSSFDIYVVGRFEMAKIARDDFVKQGALIEHIYGDAFAFL
HHHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD(P)H2; FMN [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H2; FMN; 2 2-octaprenylphenol; O2 [C]

Specific reaction: NAD(P)H2 + FMN = NAD(P) + FMNH2 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8167139; 8206831; 6981058 [H]