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The map label for this gene is dusA [H]

Identifier: 119946739

GI number: 119946739

Start: 3844081

End: 3845073

Strand: Reverse

Name: dusA [H]

Synonym: Ping_3123

Alternate gene names: 119946739

Gene position: 3845073-3844081 (Counterclockwise)

Preceding gene: 119946741

Following gene: 119946738

Centisome position: 84.33

GC content: 45.22

Gene sequence:

>993_bases
ATGATAAACCCAGATACAGCCTGCATTTACCCAAGCAACCGCTTCTCCATAGCACCGATGCTCGATTGGACGGACAGGCA
CTGCCGATACTTTCATCGTGTTATGAGTAAAAATACTTTGCTCTATAGTGAGATGGTAACGACTGGCGCTATCTTATATG
GCAAAGGGGATTACCTGCAAAAAGATGCGGCTGAACATCCGGTTTGTCTGCAATTAGGCGGATCAGCACCGGTTGATTTA
GCCACCTCGGCACTGGCAGGTAAAGAACGCGGCTTTGATGAGATTAATTTAAATGTTGGCTGCCCATCGGATCGTGTGCA
AAACGGTCGCTTTGGTGCTTGTCTGATGTCGGAAGGTGAGTTGGTTGGTGATGCCATAAAAGCCATGAAAGATGCAACGG
GTTTACCCGTCTCGGTGAAAACACGGATCGGCATTGATGATTTAGACTCCTACGCTTTTTTATGTGACTTTGTTGGTAAG
GTTGCGGAAAAAGGCTGTGATACTTTTGTCGTGCATGCACGTAAAGCCTGGTTGAGTGGTTTAAGCCCTAAACAAAACCG
TGATGTACCACCACTTGATTATGAGCGTGTATACCAACTAAAAAAAGATTTTCCGCAGTTGACCATTGCTATCAATGGCG
GTATTAAAACGCTTGATGAATGTGAAACTCATCTACAATATCTTGATGGTGTTATGTTGGGCAGAGAAATTTACACTAAT
CCTTATTTATTAAGTTCTGTTGATCAGCGCTTGTTTGCCGGCAATGAAAAAATATTAACCCGCTTTGAGGTGTTAGAAAA
GATGTATCCCTATATTGAGCAGCAACTCAGCAATGGGGCGTATTTTAAGCATGTTGCCCGTCATATGTTGGGATTATTCC
AAGGTCTGCCGGGTGCGCGGGCTTGGCGTCGCCATCTGAGTGAAAACGGCCACTTGCCGGGAGCTGGCATTGAAGTGATG
GAAAAAGCGGCGGCATTTGTTGTTGAGGGTTAA

Upstream 100 bases:

>100_bases
TTTTGAGGGGGACTATAGGAATACTATAAGTAAGGCAAAATTAACGATAATTAAACGCAATTAAACGCAAAACCAGTATA
TAGAAAAGTGAGCAATAGCA

Downstream 100 bases:

>100_bases
TTTTTAGTGGAGATAATATGAGAAAAACAGTTTTACTGGTTGGTTTTTTGGTTGCCTGGTTAAGCCTTAGCGGGTGTACT
GCCTTATCCCCAGAAGCCAA

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MINPDTACIYPSNRFSIAPMLDWTDRHCRYFHRVMSKNTLLYSEMVTTGAILYGKGDYLQKDAAEHPVCLQLGGSAPVDL
ATSALAGKERGFDEINLNVGCPSDRVQNGRFGACLMSEGELVGDAIKAMKDATGLPVSVKTRIGIDDLDSYAFLCDFVGK
VAEKGCDTFVVHARKAWLSGLSPKQNRDVPPLDYERVYQLKKDFPQLTIAINGGIKTLDECETHLQYLDGVMLGREIYTN
PYLLSSVDQRLFAGNEKILTRFEVLEKMYPYIEQQLSNGAYFKHVARHMLGLFQGLPGARAWRRHLSENGHLPGAGIEVM
EKAAAFVVEG

Sequences:

>Translated_330_residues
MINPDTACIYPSNRFSIAPMLDWTDRHCRYFHRVMSKNTLLYSEMVTTGAILYGKGDYLQKDAAEHPVCLQLGGSAPVDL
ATSALAGKERGFDEINLNVGCPSDRVQNGRFGACLMSEGELVGDAIKAMKDATGLPVSVKTRIGIDDLDSYAFLCDFVGK
VAEKGCDTFVVHARKAWLSGLSPKQNRDVPPLDYERVYQLKKDFPQLTIAINGGIKTLDECETHLQYLDGVMLGREIYTN
PYLLSSVDQRLFAGNEKILTRFEVLEKMYPYIEQQLSNGAYFKHVARHMLGLFQGLPGARAWRRHLSENGHLPGAGIEVM
EKAAAFVVEG
>Mature_330_residues
MINPDTACIYPSNRFSIAPMLDWTDRHCRYFHRVMSKNTLLYSEMVTTGAILYGKGDYLQKDAAEHPVCLQLGGSAPVDL
ATSALAGKERGFDEINLNVGCPSDRVQNGRFGACLMSEGELVGDAIKAMKDATGLPVSVKTRIGIDDLDSYAFLCDFVGK
VAEKGCDTFVVHARKAWLSGLSPKQNRDVPPLDYERVYQLKKDFPQLTIAINGGIKTLDECETHLQYLDGVMLGREIYTN
PYLLSSVDQRLFAGNEKILTRFEVLEKMYPYIEQQLSNGAYFKHVARHMLGLFQGLPGARAWRRHLSENGHLPGAGIEVM
EKAAAFVVEG

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Escherichia coli, GI145693211, Length=327, Percent_Identity=62.9969418960245, Blast_Score=452, Evalue=1e-128,
Organism=Escherichia coli, GI1788462, Length=227, Percent_Identity=30.8370044052863, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 36648; Mature: 36648

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINPDTACIYPSNRFSIAPMLDWTDRHCRYFHRVMSKNTLLYSEMVTTGAILYGKGDYLQ
CCCCCCEEEECCCCEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHCEEEECCCCHHH
KDAAEHPVCLQLGGSAPVDLATSALAGKERGFDEINLNVGCPSDRVQNGRFGACLMSEGE
HHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCCEEEECCCH
LVGDAIKAMKDATGLPVSVKTRIGIDDLDSYAFLCDFVGKVAEKGCDTFVVHARKAWLSG
HHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
LSPKQNRDVPPLDYERVYQLKKDFPQLTIAINGGIKTLDECETHLQYLDGVMLGREIYTN
CCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCC
PYLLSSVDQRLFAGNEKILTRFEVLEKMYPYIEQQLSNGAYFKHVARHMLGLFQGLPGAR
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHH
AWRRHLSENGHLPGAGIEVMEKAAAFVVEG
HHHHHHHCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MINPDTACIYPSNRFSIAPMLDWTDRHCRYFHRVMSKNTLLYSEMVTTGAILYGKGDYLQ
CCCCCCEEEECCCCEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHCEEEECCCCHHH
KDAAEHPVCLQLGGSAPVDLATSALAGKERGFDEINLNVGCPSDRVQNGRFGACLMSEGE
HHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCCEEEECCCH
LVGDAIKAMKDATGLPVSVKTRIGIDDLDSYAFLCDFVGKVAEKGCDTFVVHARKAWLSG
HHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
LSPKQNRDVPPLDYERVYQLKKDFPQLTIAINGGIKTLDECETHLQYLDGVMLGREIYTN
CCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCC
PYLLSSVDQRLFAGNEKILTRFEVLEKMYPYIEQQLSNGAYFKHVARHMLGLFQGLPGAR
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHH
AWRRHLSENGHLPGAGIEVMEKAAAFVVEG
HHHHHHHCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]