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The map label for this gene is tnsA [H]

Identifier: 119946725

GI number: 119946725

Start: 3823778

End: 3824578

Strand: Reverse

Name: tnsA [H]

Synonym: Ping_3109

Alternate gene names: 119946725

Gene position: 3824578-3823778 (Counterclockwise)

Preceding gene: 119946726

Following gene: 119946724

Centisome position: 83.88

GC content: 43.45

Gene sequence:

>801_bases
ATGGATTTGCAGCGTCAGCATAATTGGTATGTAGCTCACCGACTGAGTCTCGAATTTGAAGAAATTATGTATGACCCGTT
CTGGCGCGTGGAAGACGTCCGAAGTTCGGGTATAAAAACCAAAATCGCACATTTTTCGACACCAGGGAGGGTAATGCATC
TGCTGTCGCAGAATGAATTGTGGATGTATTTAGATTTGGCACAAAACTCAGATATTGTTGAAACCTATGAGCAGTTTGCC
ATCCCGCTCGATTTTTCTCTTCCTATAGCCAAGGAATTAGGGGTAAAGCATCCCGTTTACATCGGCAGTCAAAATGTTCC
GATTATTCAAACCATCGATTTTGTTTCTATCCGCGATGATGGCACTCGAGTGGCCCATCCTGTGAAACAGGAATCTGATG
CGTTACGTGAGCGCACCATGGAAAAATTGGCGATACAGGAGGCTTATTGCGAACTGGAATACATTGATTATCAGTTAACG
ACTAGTACTGAACTGCGCACTGTAAAAAGTGAAAGTCTTGAAGCCATGTATCGTCATCGTAATGTAAATCCATTTTTAAA
ATCCGCGTTTGAGGTTTGGCTTTCTAATTTTTTGGGGTGCCTATCAGATGACAGACATGATCGCGCTTCCAACATTCTTG
AGCGCTCTGCTCTGATAAGTGGCGTCGAATATCAACAAGCAGCTTCATTTTTTTACTGTGCTCTTTGGAATGATCGCCTT
CAGATGGATTGGAAAAAGCGATTAAAGCTCGAGTTACCCGCATCGGATCTGGGGATACACGAACATGTCCTTGTCGCTTA
A

Upstream 100 bases:

>100_bases
TCAAACTCAAAATTTCAAACAGAAAAAAAATCACCGAGTCGATGTTACCGCATTGATGATTTCGTATGAGTAGATAGTTG
TGGCAAAAAAAACTCAACGA

Downstream 100 bases:

>100_bases
TCCTAAACGCTCAGCAGAGCTTAAGTTAAATTCTGGATTTGGTCCCTCTGAAGAGGCTTTATTACCCGTTTATATCGGAG
AATATGAAATCTCTGAAGAC

Product: transposon Tn7 transposition protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MDLQRQHNWYVAHRLSLEFEEIMYDPFWRVEDVRSSGIKTKIAHFSTPGRVMHLLSQNELWMYLDLAQNSDIVETYEQFA
IPLDFSLPIAKELGVKHPVYIGSQNVPIIQTIDFVSIRDDGTRVAHPVKQESDALRERTMEKLAIQEAYCELEYIDYQLT
TSTELRTVKSESLEAMYRHRNVNPFLKSAFEVWLSNFLGCLSDDRHDRASNILERSALISGVEYQQAASFFYCALWNDRL
QMDWKKRLKLELPASDLGIHEHVLVA

Sequences:

>Translated_266_residues
MDLQRQHNWYVAHRLSLEFEEIMYDPFWRVEDVRSSGIKTKIAHFSTPGRVMHLLSQNELWMYLDLAQNSDIVETYEQFA
IPLDFSLPIAKELGVKHPVYIGSQNVPIIQTIDFVSIRDDGTRVAHPVKQESDALRERTMEKLAIQEAYCELEYIDYQLT
TSTELRTVKSESLEAMYRHRNVNPFLKSAFEVWLSNFLGCLSDDRHDRASNILERSALISGVEYQQAASFFYCALWNDRL
QMDWKKRLKLELPASDLGIHEHVLVA
>Mature_266_residues
MDLQRQHNWYVAHRLSLEFEEIMYDPFWRVEDVRSSGIKTKIAHFSTPGRVMHLLSQNELWMYLDLAQNSDIVETYEQFA
IPLDFSLPIAKELGVKHPVYIGSQNVPIIQTIDFVSIRDDGTRVAHPVKQESDALRERTMEKLAIQEAYCELEYIDYQLT
TSTELRTVKSESLEAMYRHRNVNPFLKSAFEVWLSNFLGCLSDDRHDRASNILERSALISGVEYQQAASFFYCALWNDRL
QMDWKKRLKLELPASDLGIHEHVLVA

Specific function: Required for Tn7 transposition. Forms the transposase, together with TnsB. TnsA executes the 5'-DNA strand breakage reaction. TnsABC and tnsD promote high-frequency insertion of Tn7 into a specific target site known as ATT-Tn7 whereas tnsABC and tnsD prom

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011856
- InterPro:   IPR011335
- InterPro:   IPR014832
- InterPro:   IPR014833
- InterPro:   IPR011991 [H]

Pfam domain/function: PF08721 TnsA_C; PF08722 TnsA_N [H]

EC number: NA

Molecular weight: Translated: 31044; Mature: 31044

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLQRQHNWYVAHRLSLEFEEIMYDPFWRVEDVRSSGIKTKIAHFSTPGRVMHLLSQNEL
CCCCCCCCEEEEEEECCCHHHHHCCCCHHHHHHHHCCCEEEEEECCCCHHHHHHHCCCCE
WMYLDLAQNSDIVETYEQFAIPLDFSLPIAKELGVKHPVYIGSQNVPIIQTIDFVSIRDD
EEEEEECCCCCHHHHHHHHCCCCCCCCCCHHHCCCCCCEEECCCCCCEEEEEEEEEECCC
GTRVAHPVKQESDALRERTMEKLAIQEAYCELEYIDYQLTTSTELRTVKSESLEAMYRHR
CCHHCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCHHHHHHHHHHHHHHHHHC
NVNPFLKSAFEVWLSNFLGCLSDDRHDRASNILERSALISGVEYQQAASFFYCALWNDRL
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHEEEEEEECCCH
QMDWKKRLKLELPASDLGIHEHVLVA
HHCHHHCEEEECCHHHCCCCCEEEEC
>Mature Secondary Structure
MDLQRQHNWYVAHRLSLEFEEIMYDPFWRVEDVRSSGIKTKIAHFSTPGRVMHLLSQNEL
CCCCCCCCEEEEEEECCCHHHHHCCCCHHHHHHHHCCCEEEEEECCCCHHHHHHHCCCCE
WMYLDLAQNSDIVETYEQFAIPLDFSLPIAKELGVKHPVYIGSQNVPIIQTIDFVSIRDD
EEEEEECCCCCHHHHHHHHCCCCCCCCCCHHHCCCCCCEEECCCCCCEEEEEEEEEECCC
GTRVAHPVKQESDALRERTMEKLAIQEAYCELEYIDYQLTTSTELRTVKSESLEAMYRHR
CCHHCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCHHHHHHHHHHHHHHHHHC
NVNPFLKSAFEVWLSNFLGCLSDDRHDRASNILERSALISGVEYQQAASFFYCALWNDRL
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHEEEEEEECCCH
QMDWKKRLKLELPASDLGIHEHVLVA
HHCHHHCEEEECCHHHCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2156235; 1655576; 3010949; 8947057; 10704304 [H]