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The map label for this gene is tnsB [H]

Identifier: 119946724

GI number: 119946724

Start: 3821581

End: 3823788

Strand: Reverse

Name: tnsB [H]

Synonym: Ping_3108

Alternate gene names: 119946724

Gene position: 3823788-3821581 (Counterclockwise)

Preceding gene: 119946725

Following gene: 119946723

Centisome position: 83.86

GC content: 40.35

Gene sequence:

>2208_bases
TTGTCGCTTAATCCTAAACGCTCAGCAGAGCTTAAGTTAAATTCTGGATTTGGTCCCTCTGAAGAGGCTTTATTACCCGT
TTATATCGGAGAATATGAAATCTCTGAAGACTACCTTATTGTTTTTGTTGATCTTTCAATTAATGTGTTGTGCGCCATCA
ATCTGGACTCTACTGCTCGATTTCCGATAAAACTGAATTATAAGGAGGTGTTAGCGCAGTTAGAAACTGAGCAGATTCTT
TTATTTGAGATGGATTGGCCCAAAGAAGCGTTGCAGGACATTGATAGTCAACCAGAGTGGAGGCGGGAAAAATGTATCGC
GCGCTATGCACTGATTGGACCGTTAGTAGATGACTTAGAAAATGTACTAAGGAATGATTATGGTCAGGGGTGTTTTCAAA
GCATTATTAAAGAAACAGGTAAGAGTAAGCAGACCGTCTATGATTGCTTTTACGCATTTTTGCGCATGGGAAGACGTAAA
TCGGGTCTTTCTTTTCCGATCGGAAAAAATTCAAATCACATTCCCAAGCAAAGAAAGCAGTATGTTAAGCCAGGTCGTCC
AAGTAGAGGAATCGCCAGAGGTAAGTTATTAAATGAAAGAGATTTCGATATTTTTAAAAAAGGTTTGCGCTGGTACGGAC
AGAGGAATGGCCCATCTATACTCTACGTTTATAACAAGATTTTGCGAAAACATTACCATAAATCACGTGTAAAGAATGAC
TTTCTGACAGCAGAAAAAACAGGGGAGCGCTTTAAAGTCGTGCTGCTTCCTCCTGATGAAATACCGTCTTATAACCAGTT
TTATTACTGGTTGGTAAAACAGTTTGGCGGCAACCTACCTAAACGAGACAAAAGTCGCCAAAACGCCATTGAAAATAAAA
AAGACAACGCGGGCAGAACGGGAAATGCATATATGCATGTTATCGGTCCAGGGCAAGTTTTCGAACTGGATGAAACCCCT
TTTACAGAGGAACTGGTATCGGTTTTTGACCCTTCTCGGTCAACAAAAATCGGCAAAGGGACACTCTATTTCATCATCGA
TGTGTTCACAAAGCTAATTGTCGGCCTGTTTATTACCACCGAAAATCCATCCTATAACACGGTAAAGCAAGCCATTTTTA
ATGCAGGCCGAGACAAGCAAGGATGGTTTGATGAACTGGGGTTTAACTTTAATGCTAAATACTGGCCTCAGTCTGGGGTA
CCAACAATTATTTTTGTGGATAAAGCGGAATTTCATAATAAAGTTTCCGAGGGACCTATTGCAGACCTGCCTGTGGTGTT
GAAGTTTTCCCGTTCGGGACGCGGTGATGACAAACCAAATATTGAGCAGTTATTTCATATTTTTCTACGTTATTTTATGG
GAGTAAGTCAGGGGAATCAAACAAAGTCTCAGCAAGATATTGCACGGCAAGTTGCACGTAAAAATGCGTGTTTAACTATT
CCTGAACTTTATCAAATTGCGATTGTTTACGTGCTTTATCACAACAATAATCGAAAATTACCAAAATATCCTTTAGAGCG
TGAAATGGTGCGTGACAAGGTACCTGCTATTCCAGTCAAACTGTGGGAATGGGGGATGAAAAATAGACCCGGTTATTTGA
TTAATGTCCCCGAGGATGAGCTTTATATGAAATTACTCGCCAAGGGTGAAATGACTGTTCATAAGTATGGTTTGTTCTTG
AAGGATAAGGGACTTTTGTATAACTGTGACTGGACCTTAGCCTCGGGTATACAGGAACGTAAGCTTAAAAATCAAAGGTG
TCTAACGCTTGCATGTCGTTATAACGCGGAGCTTGTTGATATTATCTTAATTTGTACCGATGACGGCCTTAAGCTCGCCA
CGCTGGATCACAAAGATAACCGTTTTGCAGGGCTCTCTTTCGCCCAAGTCGCACAACAAAAAGATAAAGAATTCAAAGAA
GATGCGTTAGCTGCGGAGGAAGAGATTCAATATCTACTCGGAGTTGATGAATTTATAGAGCAGAAACTGTTACAAGCAAA
TAAAGAAAAAGTGTCGGGTTCTGTTCCTAATATCTCCAAGATTAAAGACAACCGAAAATTTGAGGCATTGCTTAATCGAC
ACCAAGATATGCACCGTTTTCTGCAAGTCACACAACAGCAGGTTTTACCCCAGATAAAAAATCCCCCTTATGATCAAGAT
GTTTCTGAACACAAGGGATACGCAGAATTTGATGAGGATGATGAATAA

Upstream 100 bases:

>100_bases
TTTTACTGTGCTCTTTGGAATGATCGCCTTCAGATGGATTGGAAAAAGCGATTAAAGCTCGAGTTACCCGCATCGGATCT
GGGGATACACGAACATGTCC

Downstream 100 bases:

>100_bases
TGGCTATTAAGAATAAAAAATTACCAGTGACAATAACCCCCGCGCAATACCGCGATACGGAAGTTGAAGCATATAAAAAC
AACCCATTGATACTTGGGTT

Product: transposon Tn7 transposition protein B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 735; Mature: 734

Protein sequence:

>735_residues
MSLNPKRSAELKLNSGFGPSEEALLPVYIGEYEISEDYLIVFVDLSINVLCAINLDSTARFPIKLNYKEVLAQLETEQIL
LFEMDWPKEALQDIDSQPEWRREKCIARYALIGPLVDDLENVLRNDYGQGCFQSIIKETGKSKQTVYDCFYAFLRMGRRK
SGLSFPIGKNSNHIPKQRKQYVKPGRPSRGIARGKLLNERDFDIFKKGLRWYGQRNGPSILYVYNKILRKHYHKSRVKND
FLTAEKTGERFKVVLLPPDEIPSYNQFYYWLVKQFGGNLPKRDKSRQNAIENKKDNAGRTGNAYMHVIGPGQVFELDETP
FTEELVSVFDPSRSTKIGKGTLYFIIDVFTKLIVGLFITTENPSYNTVKQAIFNAGRDKQGWFDELGFNFNAKYWPQSGV
PTIIFVDKAEFHNKVSEGPIADLPVVLKFSRSGRGDDKPNIEQLFHIFLRYFMGVSQGNQTKSQQDIARQVARKNACLTI
PELYQIAIVYVLYHNNNRKLPKYPLEREMVRDKVPAIPVKLWEWGMKNRPGYLINVPEDELYMKLLAKGEMTVHKYGLFL
KDKGLLYNCDWTLASGIQERKLKNQRCLTLACRYNAELVDIILICTDDGLKLATLDHKDNRFAGLSFAQVAQQKDKEFKE
DALAAEEEIQYLLGVDEFIEQKLLQANKEKVSGSVPNISKIKDNRKFEALLNRHQDMHRFLQVTQQQVLPQIKNPPYDQD
VSEHKGYAEFDEDDE

Sequences:

>Translated_735_residues
MSLNPKRSAELKLNSGFGPSEEALLPVYIGEYEISEDYLIVFVDLSINVLCAINLDSTARFPIKLNYKEVLAQLETEQIL
LFEMDWPKEALQDIDSQPEWRREKCIARYALIGPLVDDLENVLRNDYGQGCFQSIIKETGKSKQTVYDCFYAFLRMGRRK
SGLSFPIGKNSNHIPKQRKQYVKPGRPSRGIARGKLLNERDFDIFKKGLRWYGQRNGPSILYVYNKILRKHYHKSRVKND
FLTAEKTGERFKVVLLPPDEIPSYNQFYYWLVKQFGGNLPKRDKSRQNAIENKKDNAGRTGNAYMHVIGPGQVFELDETP
FTEELVSVFDPSRSTKIGKGTLYFIIDVFTKLIVGLFITTENPSYNTVKQAIFNAGRDKQGWFDELGFNFNAKYWPQSGV
PTIIFVDKAEFHNKVSEGPIADLPVVLKFSRSGRGDDKPNIEQLFHIFLRYFMGVSQGNQTKSQQDIARQVARKNACLTI
PELYQIAIVYVLYHNNNRKLPKYPLEREMVRDKVPAIPVKLWEWGMKNRPGYLINVPEDELYMKLLAKGEMTVHKYGLFL
KDKGLLYNCDWTLASGIQERKLKNQRCLTLACRYNAELVDIILICTDDGLKLATLDHKDNRFAGLSFAQVAQQKDKEFKE
DALAAEEEIQYLLGVDEFIEQKLLQANKEKVSGSVPNISKIKDNRKFEALLNRHQDMHRFLQVTQQQVLPQIKNPPYDQD
VSEHKGYAEFDEDDE
>Mature_734_residues
SLNPKRSAELKLNSGFGPSEEALLPVYIGEYEISEDYLIVFVDLSINVLCAINLDSTARFPIKLNYKEVLAQLETEQILL
FEMDWPKEALQDIDSQPEWRREKCIARYALIGPLVDDLENVLRNDYGQGCFQSIIKETGKSKQTVYDCFYAFLRMGRRKS
GLSFPIGKNSNHIPKQRKQYVKPGRPSRGIARGKLLNERDFDIFKKGLRWYGQRNGPSILYVYNKILRKHYHKSRVKNDF
LTAEKTGERFKVVLLPPDEIPSYNQFYYWLVKQFGGNLPKRDKSRQNAIENKKDNAGRTGNAYMHVIGPGQVFELDETPF
TEELVSVFDPSRSTKIGKGTLYFIIDVFTKLIVGLFITTENPSYNTVKQAIFNAGRDKQGWFDELGFNFNAKYWPQSGVP
TIIFVDKAEFHNKVSEGPIADLPVVLKFSRSGRGDDKPNIEQLFHIFLRYFMGVSQGNQTKSQQDIARQVARKNACLTIP
ELYQIAIVYVLYHNNNRKLPKYPLEREMVRDKVPAIPVKLWEWGMKNRPGYLINVPEDELYMKLLAKGEMTVHKYGLFLK
DKGLLYNCDWTLASGIQERKLKNQRCLTLACRYNAELVDIILICTDDGLKLATLDHKDNRFAGLSFAQVAQQKDKEFKED
ALAAEEEIQYLLGVDEFIEQKLLQANKEKVSGSVPNISKIKDNRKFEALLNRHQDMHRFLQVTQQQVLPQIKNPPYDQDV
SEHKGYAEFDEDDE

Specific function: Sequence-specific, DNA-binding protein required for Tn7 transposition. Recognizes sequences necessary for recombination at both left and right ends of Tn7 and, together with tnsA, forms the transposase. TnsB executes the 5'-DNA strand breakage and joining

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 84854; Mature: 84723

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLNPKRSAELKLNSGFGPSEEALLPVYIGEYEISEDYLIVFVDLSINVLCAINLDSTAR
CCCCCCCCCEEEECCCCCCCCCCEEEEEECCEEECCCEEEEEEECCEEEEEEEECCCCCC
FPIKLNYKEVLAQLETEQILLFEMDWPKEALQDIDSQPEWRREKCIARYALIGPLVDDLE
CCEEECHHHHHHHCCCCEEEEEECCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
NVLRNDYGQGCFQSIIKETGKSKQTVYDCFYAFLRMGRRKSGLSFPIGKNSNHIPKQRKQ
HHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHH
YVKPGRPSRGIARGKLLNERDFDIFKKGLRWYGQRNGPSILYVYNKILRKHYHKSRVKND
HCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHH
FLTAEKTGERFKVVLLPPDEIPSYNQFYYWLVKQFGGNLPKRDKSRQNAIENKKDNAGRT
HCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCC
GNAYMHVIGPGQVFELDETPFTEELVSVFDPSRSTKIGKGTLYFIIDVFTKLIVGLFITT
CCEEEEEECCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHEEEEC
ENPSYNTVKQAIFNAGRDKQGWFDELGFNFNAKYWPQSGVPTIIFVDKAEFHNKVSEGPI
CCCCHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCCEEEEEECHHHHCCCCCCCC
ADLPVVLKFSRSGRGDDKPNIEQLFHIFLRYFMGVSQGNQTKSQQDIARQVARKNACLTI
CCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEH
PELYQIAIVYVLYHNNNRKLPKYPLEREMVRDKVPAIPVKLWEWGMKNRPGYLINVPEDE
HHHHHEEEEEEEEECCCCCCCCCCHHHHHHHHCCCCCCHHHHHCCCCCCCCEEEECCHHH
LYMKLLAKGEMTVHKYGLFLKDKGLLYNCDWTLASGIQERKLKNQRCLTLACRYNAELVD
HHHHHHHCCCEEEEEECEEEECCCEEEECCHHHHCCHHHHHHCCCCEEEEEEECCCCEEE
IILICTDDGLKLATLDHKDNRFAGLSFAQVAQQKDKEFKEDALAAEEEIQYLLGVDEFIE
EEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
QKLLQANKEKVSGSVPNISKIKDNRKFEALLNRHQDMHRFLQVTQQQVLPQIKNPPYDQD
HHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
VSEHKGYAEFDEDDE
HHHCCCCCCCCCCCC
>Mature Secondary Structure 
SLNPKRSAELKLNSGFGPSEEALLPVYIGEYEISEDYLIVFVDLSINVLCAINLDSTAR
CCCCCCCCEEEECCCCCCCCCCEEEEEECCEEECCCEEEEEEECCEEEEEEEECCCCCC
FPIKLNYKEVLAQLETEQILLFEMDWPKEALQDIDSQPEWRREKCIARYALIGPLVDDLE
CCEEECHHHHHHHCCCCEEEEEECCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
NVLRNDYGQGCFQSIIKETGKSKQTVYDCFYAFLRMGRRKSGLSFPIGKNSNHIPKQRKQ
HHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHH
YVKPGRPSRGIARGKLLNERDFDIFKKGLRWYGQRNGPSILYVYNKILRKHYHKSRVKND
HCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHH
FLTAEKTGERFKVVLLPPDEIPSYNQFYYWLVKQFGGNLPKRDKSRQNAIENKKDNAGRT
HCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCC
GNAYMHVIGPGQVFELDETPFTEELVSVFDPSRSTKIGKGTLYFIIDVFTKLIVGLFITT
CCEEEEEECCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHEEEEC
ENPSYNTVKQAIFNAGRDKQGWFDELGFNFNAKYWPQSGVPTIIFVDKAEFHNKVSEGPI
CCCCHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCCCCEEEEEECHHHHCCCCCCCC
ADLPVVLKFSRSGRGDDKPNIEQLFHIFLRYFMGVSQGNQTKSQQDIARQVARKNACLTI
CCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEH
PELYQIAIVYVLYHNNNRKLPKYPLEREMVRDKVPAIPVKLWEWGMKNRPGYLINVPEDE
HHHHHEEEEEEEEECCCCCCCCCCHHHHHHHHCCCCCCHHHHHCCCCCCCCEEEECCHHH
LYMKLLAKGEMTVHKYGLFLKDKGLLYNCDWTLASGIQERKLKNQRCLTLACRYNAELVD
HHHHHHHCCCEEEEEECEEEECCCEEEECCHHHHCCHHHHHHCCCCEEEEEEECCCCEEE
IILICTDDGLKLATLDHKDNRFAGLSFAQVAQQKDKEFKEDALAAEEEIQYLLGVDEFIE
EEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
QKLLQANKEKVSGSVPNISKIKDNRKFEALLNRHQDMHRFLQVTQQQVLPQIKNPPYDQD
HHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
VSEHKGYAEFDEDDE
HHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2156235; 1655576; 1657979; 8947057; 10704304 [H]