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The map label for this gene is ycgF [H]

Identifier: 119946710

GI number: 119946710

Start: 3800391

End: 3801110

Strand: Reverse

Name: ycgF [H]

Synonym: Ping_3093

Alternate gene names: 119946710

Gene position: 3801110-3800391 (Counterclockwise)

Preceding gene: 119946711

Following gene: 119946709

Centisome position: 83.37

GC content: 39.72

Gene sequence:

>720_bases
ATGATCAAAGGTATTGAATATACTCACGCATTTCAACCAATCATTAATATTGACGCTGGCAAAGTGGTTTCTTATGAAGT
GTTGTTGAGAGGAAAACATAATGAACCTCCCGGTTTCATATTTGACCAAGTGCCCACTGAAAGCTTAATGGAATTCGATC
AAGCTAATCGAGAGCGCGCTATAAAGCAAGCTTCTAATCTTGGGCTTGAGTGCAGCTTAAATTTAAACTTTACACCTGGT
TCTATTTTGTTTGAAGGCGGAAAATATGTGGAGGAGACCATTGCTGTTTCTCAATATCACGGTATAGATGCCAAACAACT
CGTCATAGAGATGACAGAATGCGAAGCAATTAATACCGCCAGCGAGTTGTCGAATGTACTAAATAAATTGCGTAGAACTG
GGGTTACAATTGCACTTGATGATTTTGGTTCCGGATATGCCGGTTTAAACTTGCTCGTAGATGTGCAGCCTGACTTGATA
AAGCTGGATATGTATTTGCTAAGGAATATTGATAAAAATGGTCCACGGCAAGCAATCGTCAGGGCTATATTTGATGTTTG
TCTGGATCTTGGGATAGATGTGCTCGCTGAAGGAGTTGAGACCGAAGAGGAATTCGACTTTTTAAATAAATTAGGAGTGT
CCTTATATCAGGGATATCTTTTTGCAAAGCCAGGGTTTGAGAGTTTTCCGCCTGTTTCAATCCAATATCTGAAAAGATGA

Upstream 100 bases:

>100_bases
TGAACGATTTTTTTACTGGAAACACGTGTCTGGTCAATATTAACGTAGGCAGAGCAAAGAAAATTCTTGATGCTTTTGCG
CAAGGGCGATGGAGATTGGA

Downstream 100 bases:

>100_bases
TCAAGCTATAATGAATAACATAGTCAGCCATAATCCTTGGCGCTCACCCTTAGGGCCAGCTAAAGCTGTTCTCATTTATT
CCTTATAAATGATTGGGAAG

Product: cyclic-di-GMP regulatory protein

Products: NA

Alternate protein names: Blrp [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MIKGIEYTHAFQPIINIDAGKVVSYEVLLRGKHNEPPGFIFDQVPTESLMEFDQANRERAIKQASNLGLECSLNLNFTPG
SILFEGGKYVEETIAVSQYHGIDAKQLVIEMTECEAINTASELSNVLNKLRRTGVTIALDDFGSGYAGLNLLVDVQPDLI
KLDMYLLRNIDKNGPRQAIVRAIFDVCLDLGIDVLAEGVETEEEFDFLNKLGVSLYQGYLFAKPGFESFPPVSIQYLKR

Sequences:

>Translated_239_residues
MIKGIEYTHAFQPIINIDAGKVVSYEVLLRGKHNEPPGFIFDQVPTESLMEFDQANRERAIKQASNLGLECSLNLNFTPG
SILFEGGKYVEETIAVSQYHGIDAKQLVIEMTECEAINTASELSNVLNKLRRTGVTIALDDFGSGYAGLNLLVDVQPDLI
KLDMYLLRNIDKNGPRQAIVRAIFDVCLDLGIDVLAEGVETEEEFDFLNKLGVSLYQGYLFAKPGFESFPPVSIQYLKR
>Mature_239_residues
MIKGIEYTHAFQPIINIDAGKVVSYEVLLRGKHNEPPGFIFDQVPTESLMEFDQANRERAIKQASNLGLECSLNLNFTPG
SILFEGGKYVEETIAVSQYHGIDAKQLVIEMTECEAINTASELSNVLNKLRRTGVTIALDDFGSGYAGLNLLVDVQPDLI
KLDMYLLRNIDKNGPRQAIVRAIFDVCLDLGIDVLAEGVETEEEFDFLNKLGVSLYQGYLFAKPGFESFPPVSIQYLKR

Specific function: Binds to and releases the YcgE repressor from its bound DNA target in a blue-light-dependent (470 nm) fashion. A shift to low temperature also triggers a YcgF-medisted relief of repression by YcgE, suggesting YcgF may serve as a thermometer. Blue light ma

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1787410, Length=231, Percent_Identity=31.6017316017316, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI87081845, Length=223, Percent_Identity=31.8385650224215, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI226510982, Length=233, Percent_Identity=31.3304721030043, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1790496, Length=241, Percent_Identity=27.8008298755187, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI87081921, Length=224, Percent_Identity=28.125, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1787541, Length=252, Percent_Identity=31.7460317460317, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1788849, Length=241, Percent_Identity=30.7053941908714, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787055, Length=233, Percent_Identity=31.7596566523605, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1786507, Length=232, Percent_Identity=28.0172413793103, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87082096, Length=127, Percent_Identity=37.7952755905512, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87081980, Length=230, Percent_Identity=26.9565217391304, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788502, Length=239, Percent_Identity=28.8702928870293, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI87081743, Length=230, Percent_Identity=26.0869565217391, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR007024
- InterPro:   IPR001633 [H]

Pfam domain/function: PF04940 BLUF; PF00563 EAL [H]

EC number: NA

Molecular weight: Translated: 26628; Mature: 26628

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKGIEYTHAFQPIINIDAGKVVSYEVLLRGKHNEPPGFIFDQVPTESLMEFDQANRERA
CCCCCCHHHHHCCEEECCCCCEEEEEEEEECCCCCCCCCEECCCCHHHHHHHHHHHHHHH
IKQASNLGLECSLNLNFTPGSILFEGGKYVEETIAVSQYHGIDAKQLVIEMTECEAINTA
HHHHHCCCEEEEEEECCCCCCEEECCCHHHHHHHHHHHHCCCCHHHHHEEHHHHHHHCCH
SELSNVLNKLRRTGVTIALDDFGSGYAGLNLLVDVQPDLIKLDMYLLRNIDKNGPRQAIV
HHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCHHHHHH
RAIFDVCLDLGIDVLAEGVETEEEFDFLNKLGVSLYQGYLFAKPGFESFPPVSIQYLKR
HHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCHHHHHCC
>Mature Secondary Structure
MIKGIEYTHAFQPIINIDAGKVVSYEVLLRGKHNEPPGFIFDQVPTESLMEFDQANRERA
CCCCCCHHHHHCCEEECCCCCEEEEEEEEECCCCCCCCCEECCCCHHHHHHHHHHHHHHH
IKQASNLGLECSLNLNFTPGSILFEGGKYVEETIAVSQYHGIDAKQLVIEMTECEAINTA
HHHHHCCCEEEEEEECCCCCCEEECCCHHHHHHHHHHHHCCCCHHHHHEEHHHHHHHCCH
SELSNVLNKLRRTGVTIALDDFGSGYAGLNLLVDVQPDLIKLDMYLLRNIDKNGPRQAIV
HHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCHHHHHH
RAIFDVCLDLGIDVLAEGVETEEEFDFLNKLGVSLYQGYLFAKPGFESFPPVSIQYLKR
HHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHCEEEECCCCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]