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The map label for this gene is nemA [H]

Identifier: 119946705

GI number: 119946705

Start: 3792043

End: 3793155

Strand: Reverse

Name: nemA [H]

Synonym: Ping_3087

Alternate gene names: 119946705

Gene position: 3793155-3792043 (Counterclockwise)

Preceding gene: 119946706

Following gene: 119946703

Centisome position: 83.19

GC content: 52.65

Gene sequence:

>1113_bases
ATGAGTAACGAAAATCAGAAAGACGTATTGTTCGATACATTTAAGCTGGGCAACCTGTCGCTGCGAAACCGCGTGCTGAT
GTCTCCGCTAACCCGCTCGCGCTCCAGCCAGCCGGGTGATATACCCAATGCGATGAACGTCCAGTACTATGAGCAGCGTG
CCAGTGCAGGCCTTATCATCAGCGAAGCGACCCAGGTATCGCCACAGGGCAAGGGCTACGCTTTTACCCCGGGCATTCAC
TCGCAAGAGCAGATTGCCGGCTGGAAGCGGGTCACCGACGCAGTTCATAAAGCGGGCGGTCTTATTCACATGCAGCTTTG
GCATGTTGGCCGGATTTCTCATCCGGACCTGCAACCCGATGGCCAAAAGCCAGTTGCTCCCTCTGCGATTGCACCCAAAG
GTGCTAAAACCTTTGTCAGTGCAACTTCCGGTATGGTGGATATTCTTGAGCCACGTGCTCTGGAAACAGATGAGATCCCC
TCTATCGTTGAGCAGTTCAGGGTTGGTGCGGAGAATGCTAAAGCCGCAGGCTTTGATGGTGTTGAAATCCATGCTGCCAA
CGGCTACCTGCTGGATCAGTTTCTTAAGAGTGACAGCAACCAGCGTACCGATCAGTATGGTGGCTCCGTCGAAAATCGTA
TCCGTCTAACCTTGATGGTAACTGAGGCCGTTGTCGAGGTCTGGGGCAAAGAAAACGTTGGTATTCGCGTTAGCCCAACC
GGGAGTTTTAACGCAGTGCATGATCAGAACCCAATTGAAACTTTTGGCACGCTGGCTAAACATCTGAACGATATCGGTAT
TGCTTATATCGAGGTGGTGGAAGACTCGTTTCAGGGCAACCATGCCAATGGTCGACCGCAGACTGTGATTGATGCTATTC
AGGCGGCTTTTAAAGGTGTATACATCGCCAATGGCGCCTACACCGCGGACGAAGCAAGAACGCGGATAGGTAAAGGCCAG
TGTGACCTGGTCACTTTTGGACGCCCATTCATCGCCAATCCGGATCTGCCTGAACGCTTCCGAACCCATGCAGAGCTTAA
CCGCTGGGACGACAGCACTTTCTACGGTGGTGACGCTGAGGGTTACATCGACTACCCAACGTTGTCTGTTTAA

Upstream 100 bases:

>100_bases
GTGTTTTTTGAACACTGCCTGCGGCCCAGCTAATTTTTTGAAAACCATTGTTAGTAAACTGGCCTAATTAATTCCAATTC
ATTTAAAAGGAAATAACACT

Downstream 100 bases:

>100_bases
CCGGGGTCTGTGGAGAATTACAGAGACCGCCAATATAAAAATAACGGCTCTGTAGGGCTTCTATTATAAGCCATGAAGGA
AAATAACCGCGAATATTAAG

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MSNENQKDVLFDTFKLGNLSLRNRVLMSPLTRSRSSQPGDIPNAMNVQYYEQRASAGLIISEATQVSPQGKGYAFTPGIH
SQEQIAGWKRVTDAVHKAGGLIHMQLWHVGRISHPDLQPDGQKPVAPSAIAPKGAKTFVSATSGMVDILEPRALETDEIP
SIVEQFRVGAENAKAAGFDGVEIHAANGYLLDQFLKSDSNQRTDQYGGSVENRIRLTLMVTEAVVEVWGKENVGIRVSPT
GSFNAVHDQNPIETFGTLAKHLNDIGIAYIEVVEDSFQGNHANGRPQTVIDAIQAAFKGVYIANGAYTADEARTRIGKGQ
CDLVTFGRPFIANPDLPERFRTHAELNRWDDSTFYGGDAEGYIDYPTLSV

Sequences:

>Translated_370_residues
MSNENQKDVLFDTFKLGNLSLRNRVLMSPLTRSRSSQPGDIPNAMNVQYYEQRASAGLIISEATQVSPQGKGYAFTPGIH
SQEQIAGWKRVTDAVHKAGGLIHMQLWHVGRISHPDLQPDGQKPVAPSAIAPKGAKTFVSATSGMVDILEPRALETDEIP
SIVEQFRVGAENAKAAGFDGVEIHAANGYLLDQFLKSDSNQRTDQYGGSVENRIRLTLMVTEAVVEVWGKENVGIRVSPT
GSFNAVHDQNPIETFGTLAKHLNDIGIAYIEVVEDSFQGNHANGRPQTVIDAIQAAFKGVYIANGAYTADEARTRIGKGQ
CDLVTFGRPFIANPDLPERFRTHAELNRWDDSTFYGGDAEGYIDYPTLSV
>Mature_369_residues
SNENQKDVLFDTFKLGNLSLRNRVLMSPLTRSRSSQPGDIPNAMNVQYYEQRASAGLIISEATQVSPQGKGYAFTPGIHS
QEQIAGWKRVTDAVHKAGGLIHMQLWHVGRISHPDLQPDGQKPVAPSAIAPKGAKTFVSATSGMVDILEPRALETDEIPS
IVEQFRVGAENAKAAGFDGVEIHAANGYLLDQFLKSDSNQRTDQYGGSVENRIRLTLMVTEAVVEVWGKENVGIRVSPTG
SFNAVHDQNPIETFGTLAKHLNDIGIAYIEVVEDSFQGNHANGRPQTVIDAIQAAFKGVYIANGAYTADEARTRIGKGQC
DLVTFGRPFIANPDLPERFRTHAELNRWDDSTFYGGDAEGYIDYPTLSV

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=375, Percent_Identity=52.5333333333333, Blast_Score=360, Evalue=1e-100,
Organism=Escherichia coli, GI1789463, Length=374, Percent_Identity=28.3422459893048, Blast_Score=115, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17559804, Length=362, Percent_Identity=28.7292817679558, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17559802, Length=298, Percent_Identity=27.8523489932886, Blast_Score=100, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17565138, Length=345, Percent_Identity=25.7971014492754, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI72001454, Length=364, Percent_Identity=27.1978021978022, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17564188, Length=360, Percent_Identity=26.9444444444444, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17540738, Length=351, Percent_Identity=25.6410256410256, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17566914, Length=349, Percent_Identity=25.214899713467, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6325086, Length=388, Percent_Identity=38.659793814433, Blast_Score=227, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6321973, Length=383, Percent_Identity=39.1644908616188, Blast_Score=221, Evalue=1e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 40429; Mature: 40297

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNENQKDVLFDTFKLGNLSLRNRVLMSPLTRSRSSQPGDIPNAMNVQYYEQRASAGLII
CCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCEEE
SEATQVSPQGKGYAFTPGIHSQEQIAGWKRVTDAVHKAGGLIHMQLWHVGRISHPDLQPD
ECCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCC
GQKPVAPSAIAPKGAKTFVSATSGMVDILEPRALETDEIPSIVEQFRVGAENAKAAGFDG
CCCCCCCCCCCCCCCHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCC
VEIHAANGYLLDQFLKSDSNQRTDQYGGSVENRIRLTLMVTEAVVEVWGKENVGIRVSPT
EEEEECCCHHHHHHHHCCCCCCHHHCCCCCCCEEEEEEEEHHHHHHHHCCCCCCEEECCC
GSFNAVHDQNPIETFGTLAKHLNDIGIAYIEVVEDSFQGNHANGRPQTVIDAIQAAFKGV
CCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEHHHCCCCCCCCCHHHHHHHHHHHHCCE
YIANGAYTADEARTRIGKGQCDLVTFGRPFIANPDLPERFRTHAELNRWDDSTFYGGDAE
EEECCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCEECCCCC
GYIDYPTLSV
CEECCCCCCC
>Mature Secondary Structure 
SNENQKDVLFDTFKLGNLSLRNRVLMSPLTRSRSSQPGDIPNAMNVQYYEQRASAGLII
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCEEE
SEATQVSPQGKGYAFTPGIHSQEQIAGWKRVTDAVHKAGGLIHMQLWHVGRISHPDLQPD
ECCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCC
GQKPVAPSAIAPKGAKTFVSATSGMVDILEPRALETDEIPSIVEQFRVGAENAKAAGFDG
CCCCCCCCCCCCCCCHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCC
VEIHAANGYLLDQFLKSDSNQRTDQYGGSVENRIRLTLMVTEAVVEVWGKENVGIRVSPT
EEEEECCCHHHHHHHHCCCCCCHHHCCCCCCCEEEEEEEEHHHHHHHHCCCCCCEEECCC
GSFNAVHDQNPIETFGTLAKHLNDIGIAYIEVVEDSFQGNHANGRPQTVIDAIQAAFKGV
CCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEHHHCCCCCCCCCHHHHHHHHHHHHCCE
YIANGAYTADEARTRIGKGQCDLVTFGRPFIANPDLPERFRTHAELNRWDDSTFYGGDAE
EEECCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCEECCCCC
GYIDYPTLSV
CEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]