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The map label for this gene is mak [H]

Identifier: 119945409

GI number: 119945409

Start: 2077076

End: 2077975

Strand: Reverse

Name: mak [H]

Synonym: Ping_1702

Alternate gene names: 119945409

Gene position: 2077975-2077076 (Counterclockwise)

Preceding gene: 119945411

Following gene: 119945403

Centisome position: 45.57

GC content: 38.89

Gene sequence:

>900_bases
ATGATTCTGGGATTAGATATTGGTGGAACAAAAATTGAAGGTGTTGGTCTTGATTCAGTGACATACGAAACACTGGTTAC
TTATCGCAATGTGACTTATACAAAGACTTATAAGGGTTTCTTGTTGTCGGTAATATCAGTCATTGATGAAGTGGCAAAGT
ACGGTAATATTGAATCTATTGGTATAGGTTGTTGCGGTTCGGTGGATAAAGCTGGACTAATGCAGGGGGCAAACCTACTC
ATTTTAAATGGTCAAGATTTTATTGGTGACATCAAAAATAAGAGTAACGTACCTGTTGCAATCGCTAATGATGCTGACTG
TTTGGCACTTTCTGAATTTAAAGATGGAGCAGCGAAAGAGGCACAGAATTCATGTGTTGCAATTATTCTTGGTACGGGTT
GTGGTTCAGGTCTAATTATTCACAATAAACTGGTTACTGGCTTGAATAATTTGGGCGGAGAACTAGGACATAGTCCGCTA
CCAAATTATTTAGCAGAAGTAGACGGTCCGGAAGTATTTTGCTACTGCGGATCTAAAAACTGTACTGAAACATTCGTTTC
TGGAACAGGTTTTGCGCGTACTTTCTCGGCACAATATCACCAGGCAAATTCAAAAGAAATTATGCATCAATACAAGCAAG
GGAATACACAGGCAGTCGTGCATCTTGATTTGTATTGTGATCAATTGGCGCGAGTTTGCGCAAATATAGTCAACTTTGTT
GATCCTGAAATGATAGTACTGGGGGGTGGAATGTCTAATATTGATGAAATTTATCCACTGGTTAATGAGAAATTAAATCA
GTATACCTTTACGAAAAAAACAGTTACCGAAATTGTTAAAAATGTTTACGGTGATTCATCCGGTGTCCGGGGGGCTGCTT
TTTTGCATCTGCAGGGATAA

Upstream 100 bases:

>100_bases
TATGAATCATTATTTGCATTTACATCTGGCTAAGTTAATTATGGTGGCGTGAGCCGTTATTACTCACAGCCTCATAAATT
GAATATTTAAGGTAGTTAAT

Downstream 100 bases:

>100_bases
AAAGATACCAGATAAAAGTAAGCGCCGGTTATTTTTTCCAATAAGTTAGTGTTTAAAAGCCCCCTCTTTAGCGCAAGGGC
CCAAATAAAACTGAAGCGGT

Product: ROK family protein

Products: NA

Alternate protein names: D-fructose kinase; Manno(fructo)kinase [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MILGLDIGGTKIEGVGLDSVTYETLVTYRNVTYTKTYKGFLLSVISVIDEVAKYGNIESIGIGCCGSVDKAGLMQGANLL
ILNGQDFIGDIKNKSNVPVAIANDADCLALSEFKDGAAKEAQNSCVAIILGTGCGSGLIIHNKLVTGLNNLGGELGHSPL
PNYLAEVDGPEVFCYCGSKNCTETFVSGTGFARTFSAQYHQANSKEIMHQYKQGNTQAVVHLDLYCDQLARVCANIVNFV
DPEMIVLGGGMSNIDEIYPLVNEKLNQYTFTKKTVTEIVKNVYGDSSGVRGAAFLHLQG

Sequences:

>Translated_299_residues
MILGLDIGGTKIEGVGLDSVTYETLVTYRNVTYTKTYKGFLLSVISVIDEVAKYGNIESIGIGCCGSVDKAGLMQGANLL
ILNGQDFIGDIKNKSNVPVAIANDADCLALSEFKDGAAKEAQNSCVAIILGTGCGSGLIIHNKLVTGLNNLGGELGHSPL
PNYLAEVDGPEVFCYCGSKNCTETFVSGTGFARTFSAQYHQANSKEIMHQYKQGNTQAVVHLDLYCDQLARVCANIVNFV
DPEMIVLGGGMSNIDEIYPLVNEKLNQYTFTKKTVTEIVKNVYGDSSGVRGAAFLHLQG
>Mature_299_residues
MILGLDIGGTKIEGVGLDSVTYETLVTYRNVTYTKTYKGFLLSVISVIDEVAKYGNIESIGIGCCGSVDKAGLMQGANLL
ILNGQDFIGDIKNKSNVPVAIANDADCLALSEFKDGAAKEAQNSCVAIILGTGCGSGLIIHNKLVTGLNNLGGELGHSPL
PNYLAEVDGPEVFCYCGSKNCTETFVSGTGFARTFSAQYHQANSKEIMHQYKQGNTQAVVHLDLYCDQLARVCANIVNFV
DPEMIVLGGGMSNIDEIYPLVNEKLNQYTFTKKTVTEIVKNVYGDSSGVRGAAFLHLQG

Specific function: Catalyzes the phosphorylation of fructose to fructose-6- P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Escherichia coli, GI87081733, Length=304, Percent_Identity=39.4736842105263, Blast_Score=204, Evalue=5e-54,
Organism=Escherichia coli, GI1787363, Length=303, Percent_Identity=35.973597359736, Blast_Score=175, Evalue=3e-45,
Organism=Escherichia coli, GI1790522, Length=308, Percent_Identity=25.3246753246753, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI87082230, Length=278, Percent_Identity=28.4172661870504, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 31931; Mature: 31931

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILGLDIGGTKIEGVGLDSVTYETLVTYRNVTYTKTYKGFLLSVISVIDEVAKYGNIESI
CEEEEECCCCEEECCCCCHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCEE
GIGCCGSVDKAGLMQGANLLILNGQDFIGDIKNKSNVPVAIANDADCLALSEFKDGAAKE
CEECCCCCCHHHHHCCCCEEEECCCHHHHHCCCCCCCCEEEECCCCEEEEHHHCCCHHHH
AQNSCVAIILGTGCGSGLIIHNKLVTGLNNLGGELGHSPLPNYLAEVDGPEVFCYCGSKN
HCCCEEEEEEECCCCCCEEEEHHHHHHHHHCCHHCCCCCCCHHHHHCCCCEEEEEECCCC
CTETFVSGTGFARTFSAQYHQANSKEIMHQYKQGNTQAVVHLDLYCDQLARVCANIVNFV
CHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHC
DPEMIVLGGGMSNIDEIYPLVNEKLNQYTFTKKTVTEIVKNVYGDSSGVRGAAFLHLQG
CCCEEEEECCCCCHHHHHHHHHHHHHHEECHHHHHHHHHHHHCCCCCCCCEEEEEEECC
>Mature Secondary Structure
MILGLDIGGTKIEGVGLDSVTYETLVTYRNVTYTKTYKGFLLSVISVIDEVAKYGNIESI
CEEEEECCCCEEECCCCCHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCEE
GIGCCGSVDKAGLMQGANLLILNGQDFIGDIKNKSNVPVAIANDADCLALSEFKDGAAKE
CEECCCCCCHHHHHCCCCEEEECCCHHHHHCCCCCCCCEEEECCCCEEEEHHHCCCHHHH
AQNSCVAIILGTGCGSGLIIHNKLVTGLNNLGGELGHSPLPNYLAEVDGPEVFCYCGSKN
HCCCEEEEEEECCCCCCEEEEHHHHHHHHHCCHHCCCCCCCHHHHHCCCCEEEEEECCCC
CTETFVSGTGFARTFSAQYHQANSKEIMHQYKQGNTQAVVHLDLYCDQLARVCANIVNFV
CHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHC
DPEMIVLGGGMSNIDEIYPLVNEKLNQYTFTKKTVTEIVKNVYGDSSGVRGAAFLHLQG
CCCEEEEECCCCCHHHHHHHHHHHHHHEECHHHHHHHHHHHHCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8807285; 9278503; 1744033 [H]