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The map label for this gene is gltX

Identifier: 119945341

GI number: 119945341

Start: 1981260

End: 1982672

Strand: Reverse

Name: gltX

Synonym: Ping_1626

Alternate gene names: 119945341

Gene position: 1982672-1981260 (Counterclockwise)

Preceding gene: 119945352

Following gene: 119945339

Centisome position: 43.48

GC content: 42.6

Gene sequence:

>1413_bases
ATGAAAGTTAAAACACGTTTTGCCCCTAGCCCCACTGGTTTTTTACATGTTGGTGGCGCGCGTACTGCGCTCTATTCCTG
GTTACATGCGAAAAGTAAAGGGGGTGAGTTTGTACTGCGCATAGAGGATACGGATATTGCGCGTTCAACTCAAGAAGCAG
TTGATGCGATCTTAGAAGGGATGACTTGGATGGGTTTGACTTGGGATGAAGGTCCTTATTACCAGACCAAACGTTTTGAT
CGCTATAAAGAAATCATAGCAAAAATGCTTTCCGATGGAACAGCTTACAAATGTTACTGCACAAAAGAACGCGTTAATGA
ACTGCGTGAAGCGCAGGAAAAGGCAGGCGAGTCACCACGCTATGATAGCAAGTGTCGAGGCTTAGCACCGCAGAACACAG
ATGCCCCTTATGTTATACGTTTCAAAAATCCTAAAGAAGGCAGTGTTAAATTTGACGATCATGTACGTGGTCCTATTGAA
TTTTCAAATACCGAGCTTGACGATTTAATTATTCAGCGTACCGATGGCACACCGACTTATAATTTTTGTGTGGTCGTTGA
TGATTGGGACATGGGTATTACTTATGTTGTTCGAGGCGAAGATCATATCAACAATACACCGCGGCAAATTAATATATTAA
AAGCATTAGGAGCCCCTCTCCCTGAATATGCGCATGTTGCTATGATTTTAGGGGATGACGGCGCAAAACTGTCTAAACGT
CATGGTGCAGTCTCTGTCATGCAATTCCGTGATGAAGGTTATTTACCTGAAGCACTTAAAAACTATCTGGTACGTTTAGG
TTGGTCACACGGTGATCAGGAAATTTTCTCAGAAAAAGAGATGATTGAGCTATTTTCCTTAGATGCGATTAATAAAGCTC
CTTCTGCATTTAATACTGAAAAATTACTTTGGTTAAATCAACATTATATTAAATCACTGGATCCGGCTTACATTGCACAG
CATCTTGCATGGCACATGACGAATCAAAAAATAGATATGACTAATGGTCCAGCCCTTCCTGAAATTGTCAGCGCACTTTC
TGAACGCGCTAAAACATTAGTTGAGTTAGCGACCACCAGCCGCTACTTCTTTGAAGAGTATGAAGACTTTGATGCTACAG
CTGCCAAGAAACATCTTCGCCCAGTGGCTGAAGATGCACTTGTTTTAGTTAAGCAAAAACTGCAAACAAGCGATGACTGG
ACAGATCCAGCACTGCATCAAATAATCAATGATACTGCCAATGAATTAGGGGTTGGTATGGGTAAAGTAGGTATGCCTCT
GCGGGTTGCTATCACAGGCGGCGGGCAATCACCTTCTTTAGATGTCACCCTGCGCTTAATTGGTAAAGAGCGTAGTATTA
AACGTATCGATCGCGCACTTGAATTTATCGCTGCGCGCGTAGCTGCTTCATAA

Upstream 100 bases:

>100_bases
TACTTTGTTTTCGAGAAAACAGAAATAGTTAATGTAATTACAATGGTATCCGTTAAAATACGCCTATTATTTTAGCGCCT
ATACATAAGGACATTTTTCA

Downstream 100 bases:

>100_bases
AAATGCTTAAATATAATTTATTTTGCAAGTTTTGATTGACAGTTAAGAGCAAGGTGCATATTATGCGCCCTGTTCTTGTT
ATACCTAGCCAATTCAAACG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MKVKTRFAPSPTGFLHVGGARTALYSWLHAKSKGGEFVLRIEDTDIARSTQEAVDAILEGMTWMGLTWDEGPYYQTKRFD
RYKEIIAKMLSDGTAYKCYCTKERVNELREAQEKAGESPRYDSKCRGLAPQNTDAPYVIRFKNPKEGSVKFDDHVRGPIE
FSNTELDDLIIQRTDGTPTYNFCVVVDDWDMGITYVVRGEDHINNTPRQINILKALGAPLPEYAHVAMILGDDGAKLSKR
HGAVSVMQFRDEGYLPEALKNYLVRLGWSHGDQEIFSEKEMIELFSLDAINKAPSAFNTEKLLWLNQHYIKSLDPAYIAQ
HLAWHMTNQKIDMTNGPALPEIVSALSERAKTLVELATTSRYFFEEYEDFDATAAKKHLRPVAEDALVLVKQKLQTSDDW
TDPALHQIINDTANELGVGMGKVGMPLRVAITGGGQSPSLDVTLRLIGKERSIKRIDRALEFIAARVAAS

Sequences:

>Translated_470_residues
MKVKTRFAPSPTGFLHVGGARTALYSWLHAKSKGGEFVLRIEDTDIARSTQEAVDAILEGMTWMGLTWDEGPYYQTKRFD
RYKEIIAKMLSDGTAYKCYCTKERVNELREAQEKAGESPRYDSKCRGLAPQNTDAPYVIRFKNPKEGSVKFDDHVRGPIE
FSNTELDDLIIQRTDGTPTYNFCVVVDDWDMGITYVVRGEDHINNTPRQINILKALGAPLPEYAHVAMILGDDGAKLSKR
HGAVSVMQFRDEGYLPEALKNYLVRLGWSHGDQEIFSEKEMIELFSLDAINKAPSAFNTEKLLWLNQHYIKSLDPAYIAQ
HLAWHMTNQKIDMTNGPALPEIVSALSERAKTLVELATTSRYFFEEYEDFDATAAKKHLRPVAEDALVLVKQKLQTSDDW
TDPALHQIINDTANELGVGMGKVGMPLRVAITGGGQSPSLDVTLRLIGKERSIKRIDRALEFIAARVAAS
>Mature_470_residues
MKVKTRFAPSPTGFLHVGGARTALYSWLHAKSKGGEFVLRIEDTDIARSTQEAVDAILEGMTWMGLTWDEGPYYQTKRFD
RYKEIIAKMLSDGTAYKCYCTKERVNELREAQEKAGESPRYDSKCRGLAPQNTDAPYVIRFKNPKEGSVKFDDHVRGPIE
FSNTELDDLIIQRTDGTPTYNFCVVVDDWDMGITYVVRGEDHINNTPRQINILKALGAPLPEYAHVAMILGDDGAKLSKR
HGAVSVMQFRDEGYLPEALKNYLVRLGWSHGDQEIFSEKEMIELFSLDAINKAPSAFNTEKLLWLNQHYIKSLDPAYIAQ
HLAWHMTNQKIDMTNGPALPEIVSALSERAKTLVELATTSRYFFEEYEDFDATAAKKHLRPVAEDALVLVKQKLQTSDDW
TDPALHQIINDTANELGVGMGKVGMPLRVAITGGGQSPSLDVTLRLIGKERSIKRIDRALEFIAARVAAS

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=328, Percent_Identity=35.0609756097561, Blast_Score=210, Evalue=2e-54,
Organism=Escherichia coli, GI1788743, Length=467, Percent_Identity=68.9507494646681, Blast_Score=697, Evalue=0.0,
Organism=Escherichia coli, GI226510925, Length=243, Percent_Identity=37.8600823045267, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1786895, Length=119, Percent_Identity=29.4117647058824, Blast_Score=70, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71993394, Length=480, Percent_Identity=30.4166666666667, Blast_Score=207, Evalue=7e-54,
Organism=Saccharomyces cerevisiae, GI6324540, Length=490, Percent_Identity=31.4285714285714, Blast_Score=247, Evalue=3e-66,
Organism=Saccharomyces cerevisiae, GI6321192, Length=229, Percent_Identity=27.9475982532751, Blast_Score=87, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6324742, Length=129, Percent_Identity=31.7829457364341, Blast_Score=73, Evalue=9e-14,
Organism=Drosophila melanogaster, GI24665321, Length=497, Percent_Identity=31.58953722334, Blast_Score=251, Evalue=8e-67,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_PSYIN (A1SVA7)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_943021.1
- ProteinModelPortal:   A1SVA7
- SMR:   A1SVA7
- STRING:   A1SVA7
- GeneID:   4623613
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_1626
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- PhylomeDB:   A1SVA7
- BioCyc:   PING357804:PING_1626-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 52808; Mature: 52808

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 239-239

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVKTRFAPSPTGFLHVGGARTALYSWLHAKSKGGEFVLRIEDTDIARSTQEAVDAILEG
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHCC
MTWMGLTWDEGPYYQTKRFDRYKEIIAKMLSDGTAYKCYCTKERVNELREAQEKAGESPR
CEEECEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHCCCCCC
YDSKCRGLAPQNTDAPYVIRFKNPKEGSVKFDDHVRGPIEFSNTELDDLIIQRTDGTPTY
CCCHHCCCCCCCCCCCEEEEECCCCCCCEEECCCCCCCCEECCCCHHHHEEEECCCCCCE
NFCVVVDDWDMGITYVVRGEDHINNTPRQINILKALGAPLPEYAHVAMILGDDGAKLSKR
EEEEEEECCCCCEEEEEECCHHCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHH
HGAVSVMQFRDEGYLPEALKNYLVRLGWSHGDQEIFSEKEMIELFSLDAINKAPSAFNTE
CCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
KLLWLNQHYIKSLDPAYIAQHLAWHMTNQKIDMTNGPALPEIVSALSERAKTLVELATTS
EEEEECHHHHHCCCHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
RYFFEEYEDFDATAAKKHLRPVAEDALVLVKQKLQTSDDWTDPALHQIINDTANELGVGM
HHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCC
GKVGMPLRVAITGGGQSPSLDVTLRLIGKERSIKRIDRALEFIAARVAAS
CCCCCCEEEEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKVKTRFAPSPTGFLHVGGARTALYSWLHAKSKGGEFVLRIEDTDIARSTQEAVDAILEG
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHCC
MTWMGLTWDEGPYYQTKRFDRYKEIIAKMLSDGTAYKCYCTKERVNELREAQEKAGESPR
CEEECEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHCCCCCC
YDSKCRGLAPQNTDAPYVIRFKNPKEGSVKFDDHVRGPIEFSNTELDDLIIQRTDGTPTY
CCCHHCCCCCCCCCCCEEEEECCCCCCCEEECCCCCCCCEECCCCHHHHEEEECCCCCCE
NFCVVVDDWDMGITYVVRGEDHINNTPRQINILKALGAPLPEYAHVAMILGDDGAKLSKR
EEEEEEECCCCCEEEEEECCHHCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHH
HGAVSVMQFRDEGYLPEALKNYLVRLGWSHGDQEIFSEKEMIELFSLDAINKAPSAFNTE
CCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCC
KLLWLNQHYIKSLDPAYIAQHLAWHMTNQKIDMTNGPALPEIVSALSERAKTLVELATTS
EEEEECHHHHHCCCHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
RYFFEEYEDFDATAAKKHLRPVAEDALVLVKQKLQTSDDWTDPALHQIINDTANELGVGM
HHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCC
GKVGMPLRVAITGGGQSPSLDVTLRLIGKERSIKRIDRALEFIAARVAAS
CCCCCCEEEEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA