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The map label for this gene is metG

Identifier: 119945339

GI number: 119945339

Start: 1976194

End: 1978254

Strand: Reverse

Name: metG

Synonym: Ping_1623

Alternate gene names: 119945339

Gene position: 1978254-1976194 (Counterclockwise)

Preceding gene: 119945341

Following gene: 119945336

Centisome position: 43.39

GC content: 42.26

Gene sequence:

>2061_bases
ATGGAAATTCAACAACGTAAAATCCTTGTTACCTGCGCCCTACCCTATGCAAACGGTCCGATTCATCTCGGACACATGCT
TGAACATGTTCAAGCTGATATCTGGGTTCGTTTTCAGCGTTTACGTGGTCACAATATCCATTTTATTTGTGCAGATGATG
CGCACGGCACACCTATTATGCTTAAAGCACAAGAGATGGGTATTAAGCCGACCGATATGATAGCGGACATTCGAGCTTCT
CACGAAAGTGATTTTTCCGGGTTTAATATTAGCTTTGATAACTACCACAGCACCCATAGCGAAGAGAACCAGGCGTTGTC
TTCACAAATTTATATCGCACTACGTGAAAATGGTTATATTACCAATAGAACTATCTCACAATTATTTGATCCTGAAAAAA
AGATGTTTTTACCGGACCGTTTTGTCAAAGGGACCTGTCCTGACTGTAAAGCTGACGATCAATACGGCGATAATTGTGAC
GCTTGTGGCGCAACGTATAGCCCAACAGAGATGATTAACCCCAAATCAGCGGTTTCAGGCAGCACGCCCATTATGAAAGA
CACTGAGCATTTCTTTTTCGATCTGCCGCAATTTGAAGATATGTTAAAAGTCTGGACTAAATCAGGTGCGCTACAGACAG
AGATGGCAAATAAAGTCGGTGAATGGTTTGAAACCGGTCTAAAGCAGTGGGATATTACCCGTGATGCGCCTTACTTTGGT
TTTGAAATACCCGATGCACCGGGTAAATTTTTCTATGTTTGGTTAGACGCACCGATTGGTTACATGGGCAGCTTTAAAAA
CCTCTGTGATAAAACCGAGGGACTTAACTTTGATGATTATTGGAAAAAAGACAGCGCAGCTGAGTTATACCATTTCATCG
GTAAAGACATTGTTAACTTCCACAGCCTATTTTGGCCAGCTGTTTTAGAAGGTGCCGGATTCCGTAAACCAACGGCTGTT
AATGTACACGGTTATGTCACAGTAAATGGCGCGAAAATGTCAAAATCTAAAGGCACTTTCATTAAAGCCAGCACCTACTT
AGATAACCTGGATCCTGAGTGTTTACGTTATTATTATGCAGCTAAATTAACCAGTCGCATTGACGATTTAGATTTAAACC
TTGAAGATTTTACACAGAGGGTAAACTCCGATGTGGTTAACAAACTGGTTAATCTTGCATCACGTACAGCGGGTTTTATT
ACTAAAAAATACGATGGTCAATTATCAAACCATGTTTCTGAACCGCAGTTATATCAATCATTTATTGATGCAGGGACAAG
CATTGCAGCATTATTTGAACAACGTGATTTTGCCCGTGCGATTCGTGAAATAATGGCATTGGCTGATATCGCTAACAAAT
ACATTGATGAAAAAGCACCCTGGGCATTAGCGAAACAGGAAGGCAGCGAAGTGAAAGTGCAGGAGATCTGCTCAATGGGG
ATCAACTTATTCCGTGTTCTAATGACTTATCTAAAACCTATTATCCCAAAACTGGCGGCACGCAGTGAGGACTTTCTGGC
GGAAACACTGGATTGGGAAATCATTAAAAAGCCTCTTGCCGGTCACCGCATCAATAAATTCAAAGCGTTATTCCAACGTA
TTGATCCTAAACATGTAGAAGCCATGGTTGACGCATCGAAAGACAGTCTGGAAGCGGATAAAGCTGCCAAGCAGAAACAA
GCAGCAGCACAACTTGATGCTGCAAAATCAGAACTGGATAAAGAGCCGCTGGCACCGGAAATTGACTTTGATACTTTTGC
TAAAACAGACTTACGTGTCGCTCTAATCACAAAAGCGGAAGCAGTACCTAAGGCTAACAAATTACTTAAACTAACCTTAG
ATTTAGGCGGCGAAACACGCACAGTATTCGCCGGTATTAAGTCAGCCTATACGCCTGAAGAATTAGAGGGCAGGTTAACG
GTCATGGTTGCGAACTTAGCGCCACGTCAAATGAAGTTCGGTTTATCAGAAGGCATGGTATTAGCAACGGGTCCTGGCGG
TAAAGAGATCCACATTCTAAATCCAGATGATGGTGCAAAACCGGGCCAACGTATAATGTAA

Upstream 100 bases:

>100_bases
AGGATTTTTCAAGCCCATTTAAGAGTGGGATTTCAAGCCTTTTTTGGGTAGAATCGCAACCTTAAAATTTAGATTTAATA
CAACAGGAATAATAAATAAA

Downstream 100 bases:

>100_bases
GTCTTAGGCCCATTTCGTTTTTAGCAAAATAATGCTTGTCAGTTAAAGCCGAAAAAAAATGTAAAAGCCCTAAACATATT
AATTGTTTGGGGCTTTTTTA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 686; Mature: 686

Protein sequence:

>686_residues
MEIQQRKILVTCALPYANGPIHLGHMLEHVQADIWVRFQRLRGHNIHFICADDAHGTPIMLKAQEMGIKPTDMIADIRAS
HESDFSGFNISFDNYHSTHSEENQALSSQIYIALRENGYITNRTISQLFDPEKKMFLPDRFVKGTCPDCKADDQYGDNCD
ACGATYSPTEMINPKSAVSGSTPIMKDTEHFFFDLPQFEDMLKVWTKSGALQTEMANKVGEWFETGLKQWDITRDAPYFG
FEIPDAPGKFFYVWLDAPIGYMGSFKNLCDKTEGLNFDDYWKKDSAAELYHFIGKDIVNFHSLFWPAVLEGAGFRKPTAV
NVHGYVTVNGAKMSKSKGTFIKASTYLDNLDPECLRYYYAAKLTSRIDDLDLNLEDFTQRVNSDVVNKLVNLASRTAGFI
TKKYDGQLSNHVSEPQLYQSFIDAGTSIAALFEQRDFARAIREIMALADIANKYIDEKAPWALAKQEGSEVKVQEICSMG
INLFRVLMTYLKPIIPKLAARSEDFLAETLDWEIIKKPLAGHRINKFKALFQRIDPKHVEAMVDASKDSLEADKAAKQKQ
AAAQLDAAKSELDKEPLAPEIDFDTFAKTDLRVALITKAEAVPKANKLLKLTLDLGGETRTVFAGIKSAYTPEELEGRLT
VMVANLAPRQMKFGLSEGMVLATGPGGKEIHILNPDDGAKPGQRIM

Sequences:

>Translated_686_residues
MEIQQRKILVTCALPYANGPIHLGHMLEHVQADIWVRFQRLRGHNIHFICADDAHGTPIMLKAQEMGIKPTDMIADIRAS
HESDFSGFNISFDNYHSTHSEENQALSSQIYIALRENGYITNRTISQLFDPEKKMFLPDRFVKGTCPDCKADDQYGDNCD
ACGATYSPTEMINPKSAVSGSTPIMKDTEHFFFDLPQFEDMLKVWTKSGALQTEMANKVGEWFETGLKQWDITRDAPYFG
FEIPDAPGKFFYVWLDAPIGYMGSFKNLCDKTEGLNFDDYWKKDSAAELYHFIGKDIVNFHSLFWPAVLEGAGFRKPTAV
NVHGYVTVNGAKMSKSKGTFIKASTYLDNLDPECLRYYYAAKLTSRIDDLDLNLEDFTQRVNSDVVNKLVNLASRTAGFI
TKKYDGQLSNHVSEPQLYQSFIDAGTSIAALFEQRDFARAIREIMALADIANKYIDEKAPWALAKQEGSEVKVQEICSMG
INLFRVLMTYLKPIIPKLAARSEDFLAETLDWEIIKKPLAGHRINKFKALFQRIDPKHVEAMVDASKDSLEADKAAKQKQ
AAAQLDAAKSELDKEPLAPEIDFDTFAKTDLRVALITKAEAVPKANKLLKLTLDLGGETRTVFAGIKSAYTPEELEGRLT
VMVANLAPRQMKFGLSEGMVLATGPGGKEIHILNPDDGAKPGQRIM
>Mature_686_residues
MEIQQRKILVTCALPYANGPIHLGHMLEHVQADIWVRFQRLRGHNIHFICADDAHGTPIMLKAQEMGIKPTDMIADIRAS
HESDFSGFNISFDNYHSTHSEENQALSSQIYIALRENGYITNRTISQLFDPEKKMFLPDRFVKGTCPDCKADDQYGDNCD
ACGATYSPTEMINPKSAVSGSTPIMKDTEHFFFDLPQFEDMLKVWTKSGALQTEMANKVGEWFETGLKQWDITRDAPYFG
FEIPDAPGKFFYVWLDAPIGYMGSFKNLCDKTEGLNFDDYWKKDSAAELYHFIGKDIVNFHSLFWPAVLEGAGFRKPTAV
NVHGYVTVNGAKMSKSKGTFIKASTYLDNLDPECLRYYYAAKLTSRIDDLDLNLEDFTQRVNSDVVNKLVNLASRTAGFI
TKKYDGQLSNHVSEPQLYQSFIDAGTSIAALFEQRDFARAIREIMALADIANKYIDEKAPWALAKQEGSEVKVQEICSMG
INLFRVLMTYLKPIIPKLAARSEDFLAETLDWEIIKKPLAGHRINKFKALFQRIDPKHVEAMVDASKDSLEADKAAKQKQ
AAAQLDAAKSELDKEPLAPEIDFDTFAKTDLRVALITKAEAVPKANKLLKLTLDLGGETRTVFAGIKSAYTPEELEGRLT
VMVANLAPRQMKFGLSEGMVLATGPGGKEIHILNPDDGAKPGQRIM

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI14043022, Length=651, Percent_Identity=29.3394777265745, Blast_Score=260, Evalue=3e-69,
Organism=Homo sapiens, GI24308436, Length=511, Percent_Identity=24.853228962818, Blast_Score=146, Evalue=8e-35,
Organism=Escherichia coli, GI1788432, Length=682, Percent_Identity=67.8885630498534, Blast_Score=1015, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17540794, Length=600, Percent_Identity=28.6666666666667, Blast_Score=252, Evalue=5e-67,
Organism=Caenorhabditis elegans, GI212645096, Length=521, Percent_Identity=23.9923224568138, Blast_Score=141, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71992781, Length=410, Percent_Identity=24.390243902439, Blast_Score=108, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6321704, Length=559, Percent_Identity=29.695885509839, Blast_Score=254, Evalue=3e-68,
Organism=Saccharomyces cerevisiae, GI6321610, Length=548, Percent_Identity=22.0802919708029, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24655311, Length=644, Percent_Identity=28.7267080745342, Blast_Score=248, Evalue=9e-66,
Organism=Drosophila melanogaster, GI21358483, Length=519, Percent_Identity=25.2408477842004, Blast_Score=146, Evalue=6e-35,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_PSYIN (A1SVA5)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_943019.1
- ProteinModelPortal:   A1SVA5
- STRING:   A1SVA5
- GeneID:   4623609
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_1623
- eggNOG:   COG0143
- HOGENOM:   HBG721667
- OMA:   SAYQPEQ
- PhylomeDB:   A1SVA5
- BioCyc:   PING357804:PING_1623-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 76891; Mature: 76891

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 335-335

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIQQRKILVTCALPYANGPIHLGHMLEHVQADIWVRFQRLRGHNIHFICADDAHGTPIM
CCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEE
LKAQEMGIKPTDMIADIRASHESDFSGFNISFDNYHSTHSEENQALSSQIYIALRENGYI
EEHHHCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHCCEEEEEEECCCEE
TNRTISQLFDPEKKMFLPDRFVKGTCPDCKADDQYGDNCDACGATYSPTEMINPKSAVSG
EHHHHHHHHCCCHHCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCHHHCCC
STPIMKDTEHFFFDLPQFEDMLKVWTKSGALQTEMANKVGEWFETGLKQWDITRDAPYFG
CCCCCCCCHHHEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
FEIPDAPGKFFYVWLDAPIGYMGSFKNLCDKTEGLNFDDYWKKDSAAELYHFIGKDIVNF
EECCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHH
HSLFWPAVLEGAGFRKPTAVNVHGYVTVNGAKMSKSKGTFIKASTYLDNLDPECLRYYYA
HHHHHHHHHCCCCCCCCEEEEEEEEEEECCCEECCCCCCEEEEEHHHCCCCHHHHHHHHH
AKLTSRIDDLDLNLEDFTQRVNSDVVNKLVNLASRTAGFITKKYDGQLSNHVSEPQLYQS
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHCCCCHHHHHH
FIDAGTSIAALFEQRDFARAIREIMALADIANKYIDEKAPWALAKQEGSEVKVQEICSMG
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHH
INLFRVLMTYLKPIIPKLAARSEDFLAETLDWEIIKKPLAGHRINKFKALFQRIDPKHVE
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHH
AMVDASKDSLEADKAAKQKQAAAQLDAAKSELDKEPLAPEIDFDTFAKTDLRVALITKAE
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCEEEEEEECHH
AVPKANKLLKLTLDLGGETRTVFAGIKSAYTPEELEGRLTVMVANLAPRQMKFGLSEGMV
CCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCHHHCCEEEEEEECCCCHHHHCCCCCCEE
LATGPGGKEIHILNPDDGAKPGQRIM
EEECCCCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MEIQQRKILVTCALPYANGPIHLGHMLEHVQADIWVRFQRLRGHNIHFICADDAHGTPIM
CCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEE
LKAQEMGIKPTDMIADIRASHESDFSGFNISFDNYHSTHSEENQALSSQIYIALRENGYI
EEHHHCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHCCEEEEEEECCCEE
TNRTISQLFDPEKKMFLPDRFVKGTCPDCKADDQYGDNCDACGATYSPTEMINPKSAVSG
EHHHHHHHHCCCHHCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCHHHCCC
STPIMKDTEHFFFDLPQFEDMLKVWTKSGALQTEMANKVGEWFETGLKQWDITRDAPYFG
CCCCCCCCHHHEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
FEIPDAPGKFFYVWLDAPIGYMGSFKNLCDKTEGLNFDDYWKKDSAAELYHFIGKDIVNF
EECCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHH
HSLFWPAVLEGAGFRKPTAVNVHGYVTVNGAKMSKSKGTFIKASTYLDNLDPECLRYYYA
HHHHHHHHHCCCCCCCCEEEEEEEEEEECCCEECCCCCCEEEEEHHHCCCCHHHHHHHHH
AKLTSRIDDLDLNLEDFTQRVNSDVVNKLVNLASRTAGFITKKYDGQLSNHVSEPQLYQS
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHCCCCHHHHHH
FIDAGTSIAALFEQRDFARAIREIMALADIANKYIDEKAPWALAKQEGSEVKVQEICSMG
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHH
INLFRVLMTYLKPIIPKLAARSEDFLAETLDWEIIKKPLAGHRINKFKALFQRIDPKHVE
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHH
AMVDASKDSLEADKAAKQKQAAAQLDAAKSELDKEPLAPEIDFDTFAKTDLRVALITKAE
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCEEEEEEECHH
AVPKANKLLKLTLDLGGETRTVFAGIKSAYTPEELEGRLTVMVANLAPRQMKFGLSEGMV
CCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCHHHCCEEEEEEECCCCHHHHCCCCCCEE
LATGPGGKEIHILNPDDGAKPGQRIM
EEECCCCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA