Click here to switch to the map view.

The map label for this gene is smpB

Identifier: 119945318

GI number: 119945318

Start: 1958394

End: 1958879

Strand: Reverse

Name: smpB

Synonym: Ping_1602

Alternate gene names: 119945318

Gene position: 1958879-1958394 (Counterclockwise)

Preceding gene: 119945321

Following gene: 119945317

Centisome position: 42.96

GC content: 41.15

Gene sequence:

>486_bases
ATGGCTAAAAAAAATTCAAAAACTAAAAACAATGACAATACGATTGCACGAAATAAACGTGCTAGTCACGACTACCACCT
AGAAGAGCGCTTTGAAGCAGGTCTTGAATTACAGGGTTGGGAAGTAAAATCGTTACGCGCGGGCAAAGCGAATATCGCTG
ATAGTTATATTTTCCTGAAAAATGGAGAAGCATGGCTACTGGGTGCCACTTTTCCTCCTCTTCTTGTCGCATCTTCACAT
GTTGTATGTGATCCTATGCGTTATCGTAAATTACTGCTTAACAGGCGAGAACTCGACAACTTAGTCGGTAAAGTTGAACG
TCAGGGTTACTCTATTATTCCCATCTCTTTATACTGGAAACACGCGTGGGTAAAAATATCATTTGCACTGGCAAAAGGTA
AGCAAGAACACGACAAACGTGCTGATGTGAAAGATAGGGAATGGAAAGTAGACAAAGAACGTATGATGAAACACAGCGTT
CGCTAG

Upstream 100 bases:

>100_bases
AGGATTTAATCCTAAATTTAAGCGATTTTTATACAAATCAATGAGTAATTTATAATAGTCACTCTATCAATTTATAATAG
TCACTCTATAATACGAGACT

Downstream 100 bases:

>100_bases
CAACAAATGTGAAAATTAATTAATAGCAAAATAATGTGATATAGATAACAATAACACTATCTAAAGAATATTTTTTTTGC
TATCATTAAAACGTAAGCAG

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 161; Mature: 160

Protein sequence:

>161_residues
MAKKNSKTKNNDNTIARNKRASHDYHLEERFEAGLELQGWEVKSLRAGKANIADSYIFLKNGEAWLLGATFPPLLVASSH
VVCDPMRYRKLLLNRRELDNLVGKVERQGYSIIPISLYWKHAWVKISFALAKGKQEHDKRADVKDREWKVDKERMMKHSV
R

Sequences:

>Translated_161_residues
MAKKNSKTKNNDNTIARNKRASHDYHLEERFEAGLELQGWEVKSLRAGKANIADSYIFLKNGEAWLLGATFPPLLVASSH
VVCDPMRYRKLLLNRRELDNLVGKVERQGYSIIPISLYWKHAWVKISFALAKGKQEHDKRADVKDREWKVDKERMMKHSV
R
>Mature_160_residues
AKKNSKTKNNDNTIARNKRASHDYHLEERFEAGLELQGWEVKSLRAGKANIADSYIFLKNGEAWLLGATFPPLLVASSHV
VCDPMRYRKLLLNRRELDNLVGKVERQGYSIIPISLYWKHAWVKISFALAKGKQEHDKRADVKDREWKVDKERMMKHSVR

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=161, Percent_Identity=62.7329192546584, Blast_Score=219, Evalue=7e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_PSYIN (A1SV84)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_942998.1
- ProteinModelPortal:   A1SV84
- SMR:   A1SV84
- STRING:   A1SV84
- GeneID:   4624631
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_1602
- eggNOG:   COG0691
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- PhylomeDB:   A1SV84
- ProtClustDB:   PRK05422
- BioCyc:   PING357804:PING_1602-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 18746; Mature: 18615

Theoretical pI: Translated: 10.50; Mature: 10.50

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKNSKTKNNDNTIARNKRASHDYHLEERFEAGLELQGWEVKSLRAGKANIADSYIFLK
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCEECCCEECCCCCCCCCCCCCEEEEE
NGEAWLLGATFPPLLVASSHVVCDPMRYRKLLLNRRELDNLVGKVERQGYSIIPISLYWK
CCCEEEEECCCCHHHHCCCCEEECHHHHHHHHHCHHHHHHHHHHHHHCCCEEEEEEEEEE
HAWVKISFALAKGKQEHDKRADVKDREWKVDKERMMKHSVR
EEEEEEEEEECCCCHHHHHCCCCCCCCCCCCHHHHHHHCCC
>Mature Secondary Structure 
AKKNSKTKNNDNTIARNKRASHDYHLEERFEAGLELQGWEVKSLRAGKANIADSYIFLK
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCEECCCEECCCCCCCCCCCCCEEEEE
NGEAWLLGATFPPLLVASSHVVCDPMRYRKLLLNRRELDNLVGKVERQGYSIIPISLYWK
CCCEEEEECCCCHHHHCCCCEEECHHHHHHHHHCHHHHHHHHHHHHHCCCEEEEEEEEEE
HAWVKISFALAKGKQEHDKRADVKDREWKVDKERMMKHSVR
EEEEEEEEEECCCCHHHHHCCCCCCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA