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The map label for this gene is aroA

Identifier: 119944874

GI number: 119944874

Start: 1400837

End: 1402123

Strand: Direct

Name: aroA

Synonym: Ping_1117

Alternate gene names: 119944874

Gene position: 1400837-1402123 (Clockwise)

Preceding gene: 119944873

Following gene: 119944875

Centisome position: 30.72

GC content: 42.19

Gene sequence:

>1287_bases
ATGGAACAATTATTATTACAGCCAATTGCTAAGGTGGACGGTGAAATTAACCTACCTGGCTCTAAGAGCCTTTCTAATCG
TGCGCTTTTGCTGGCCGCTTTAGCGCAGGGTACAACGACACTGACCAACCTGTTAGACAGTGATGATATTCGTCATATGT
TAAATGCATTAAAAAAATTAGGGGTCAATTATCAGCTTTCAAAGGATAAAAAACAGTGTGTTGTTGAAGGTTTAGGACGG
GCGTTTAATACTGCTGAATCAGGTCTATTGGAACTCTTTTTAGGCAATGCCGGCACAGCGATGCGGCCGCTATGTGCCGC
TTTGTGTTTAGGTCAAGGAGAATACATTCTAACCGGTGAGGCGCGAATGTTTGAACGTCCTATCGGCAGTTTAGTGGATG
CATTACAGCAGGCCGGTGCACAGGTGACATACCTTGAAAATGAAAATTATCCGCCCTTAAAAATCAAAGGAACGGGGCTT
AAGGGCGGTAAAATAAAAATTGACGGCAGTGTTTCCAGTCAATTTTTAACCGCTTTTTTAATGGCCGCACCGATGGCAAT
GGAAGATACTGAAATTGAAATAGTCGGTGAATTGGTGTCAAAACCTTATATTAAAATCACCCTGCAAATCATGCATGATT
TTGGCATTGATGTTGATAATCATAATTTCGAACGTTTTATTATTAAAGGCAAACAAACTTATAGTGCGCCCGGGCATTAT
TTAGTTGAAGGAGACGCCTCTTCAGCTTCCTATTTTTTAGCCGCAGGCGCGATTGCCGGGGGCTGTATTAAAGTGACTGG
TATCGGTAAAAAATCGGTTCAGGGTGATATTCAATTTGCCGATGCATTGGAAGCAATGGGGGCAAAAATAGAATGGGGTG
ATGATTATATTAAAGCCAGTAAAGGTGAGTTAAAAGCCATTGATATGGATATGAATCACATTCCTGATGCCGCAATGACG
ATTGCTGTTGCCGCTCTCTTCGCAACGGGTACGACCAAAATTCGCAACGTTTATAACTGGCGCGTTAAAGAGACCGACCG
GTTATACGCGATGGCGACAGAATTACGTAAAGTTGGCGCAACCGTTGTTGAAGGGCATGATTTTATTGAGGTCACTGCGC
CCGAAAAATTAATTCATGCTGAAATTGATACCTATGACGATCACCGAATGGCGATGTGTTTTTCATTAGTGGCGTTGGGT
AATACACAGGTGACTATTAACGATCCTAAATGTACCTCAAAAACCTTTCCTGATTATTTTGAAAAGTTGGCTTCTGTGAG
TTGCTAA

Upstream 100 bases:

>100_bases
GCGACCATCTGCGTATTAGCATCGGCCTGTTCGATGAAAACCAGCGTTTTAAACAAGCCTTAGTTAAAGTGCTTAAGTTA
GATCAAAAATAGAGGATAGG

Downstream 100 bases:

>100_bases
TCAATAGCGCTTACCCATAATCACGCCCTGCAGTGCAGGGCGTGATTATGTTTTTCTGTTCTTTTCATTTCCCATTATTC
CCATGCCTGTTTTTCGGTAG

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MEQLLLQPIAKVDGEINLPGSKSLSNRALLLAALAQGTTTLTNLLDSDDIRHMLNALKKLGVNYQLSKDKKQCVVEGLGR
AFNTAESGLLELFLGNAGTAMRPLCAALCLGQGEYILTGEARMFERPIGSLVDALQQAGAQVTYLENENYPPLKIKGTGL
KGGKIKIDGSVSSQFLTAFLMAAPMAMEDTEIEIVGELVSKPYIKITLQIMHDFGIDVDNHNFERFIIKGKQTYSAPGHY
LVEGDASSASYFLAAGAIAGGCIKVTGIGKKSVQGDIQFADALEAMGAKIEWGDDYIKASKGELKAIDMDMNHIPDAAMT
IAVAALFATGTTKIRNVYNWRVKETDRLYAMATELRKVGATVVEGHDFIEVTAPEKLIHAEIDTYDDHRMAMCFSLVALG
NTQVTINDPKCTSKTFPDYFEKLASVSC

Sequences:

>Translated_428_residues
MEQLLLQPIAKVDGEINLPGSKSLSNRALLLAALAQGTTTLTNLLDSDDIRHMLNALKKLGVNYQLSKDKKQCVVEGLGR
AFNTAESGLLELFLGNAGTAMRPLCAALCLGQGEYILTGEARMFERPIGSLVDALQQAGAQVTYLENENYPPLKIKGTGL
KGGKIKIDGSVSSQFLTAFLMAAPMAMEDTEIEIVGELVSKPYIKITLQIMHDFGIDVDNHNFERFIIKGKQTYSAPGHY
LVEGDASSASYFLAAGAIAGGCIKVTGIGKKSVQGDIQFADALEAMGAKIEWGDDYIKASKGELKAIDMDMNHIPDAAMT
IAVAALFATGTTKIRNVYNWRVKETDRLYAMATELRKVGATVVEGHDFIEVTAPEKLIHAEIDTYDDHRMAMCFSLVALG
NTQVTINDPKCTSKTFPDYFEKLASVSC
>Mature_428_residues
MEQLLLQPIAKVDGEINLPGSKSLSNRALLLAALAQGTTTLTNLLDSDDIRHMLNALKKLGVNYQLSKDKKQCVVEGLGR
AFNTAESGLLELFLGNAGTAMRPLCAALCLGQGEYILTGEARMFERPIGSLVDALQQAGAQVTYLENENYPPLKIKGTGL
KGGKIKIDGSVSSQFLTAFLMAAPMAMEDTEIEIVGELVSKPYIKITLQIMHDFGIDVDNHNFERFIIKGKQTYSAPGHY
LVEGDASSASYFLAAGAIAGGCIKVTGIGKKSVQGDIQFADALEAMGAKIEWGDDYIKASKGELKAIDMDMNHIPDAAMT
IAVAALFATGTTKIRNVYNWRVKETDRLYAMATELRKVGATVVEGHDFIEVTAPEKLIHAEIDTYDDHRMAMCFSLVALG
NTQVTINDPKCTSKTFPDYFEKLASVSC

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family

Homologues:

Organism=Escherichia coli, GI1787137, Length=427, Percent_Identity=70.7259953161593, Blast_Score=615, Evalue=1e-177,
Organism=Escherichia coli, GI1789580, Length=301, Percent_Identity=27.2425249169435, Blast_Score=62, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6320332, Length=450, Percent_Identity=39.5555555555556, Blast_Score=256, Evalue=3e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROA_PSYIN (A1STZ0)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_942554.1
- ProteinModelPortal:   A1STZ0
- SMR:   A1STZ0
- STRING:   A1STZ0
- GeneID:   4626247
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_1117
- eggNOG:   COG0128
- HOGENOM:   HBG646626
- OMA:   RIAAMAC
- PhylomeDB:   A1STZ0
- BioCyc:   PING357804:PING_1117-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00210
- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.10
- PIRSF:   PIRSF000505
- TIGRFAMs:   TIGR01356

Pfam domain/function: PF00275 EPSP_synthase; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =2.5.1.19

Molecular weight: Translated: 46363; Mature: 46363

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00104 EPSP_SYNTHASE_1; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQLLLQPIAKVDGEINLPGSKSLSNRALLLAALAQGTTTLTNLLDSDDIRHMLNALKKL
CCHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHC
GVNYQLSKDKKQCVVEGLGRAFNTAESGLLELFLGNAGTAMRPLCAALCLGQGEYILTGE
CCCEEECCHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHHHCCCCCEEEECC
ARMFERPIGSLVDALQQAGAQVTYLENENYPPLKIKGTGLKGGKIKIDGSVSSQFLTAFL
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEECCCCCCEEEECCCCHHHHHHHHH
MAAPMAMEDTEIEIVGELVSKPYIKITLQIMHDFGIDVDNHNFERFIIKGKQTYSAPGHY
HHCCCCCCCCHHHHHHHHHCCCEEEEEEEEHHHHCCCCCCCCCEEEEEECCEECCCCCCE
LVEGDASSASYFLAAGAIAGGCIKVTGIGKKSVQGDIQFADALEAMGAKIEWGDDYIKAS
EEECCCCCCEEEEEHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCCCEEEECCHHHCCC
KGELKAIDMDMNHIPDAAMTIAVAALFATGTTKIRNVYNWRVKETDRLYAMATELRKVGA
CCCEEEEECCHHHCCHHHHHHHHHHHHHCCCHHHHHHEEEEECCCHHHHHHHHHHHHHCC
TVVEGHDFIEVTAPEKLIHAEIDTYDDHRMAMCFSLVALGNTQVTINDPKCTSKTFPDYF
EEECCCCEEEEECCHHEEEEECCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHH
EKLASVSC
HHHHCCCC
>Mature Secondary Structure
MEQLLLQPIAKVDGEINLPGSKSLSNRALLLAALAQGTTTLTNLLDSDDIRHMLNALKKL
CCHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHC
GVNYQLSKDKKQCVVEGLGRAFNTAESGLLELFLGNAGTAMRPLCAALCLGQGEYILTGE
CCCEEECCHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHHHCCCCCEEEECC
ARMFERPIGSLVDALQQAGAQVTYLENENYPPLKIKGTGLKGGKIKIDGSVSSQFLTAFL
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEECCCCCCEEEECCCCHHHHHHHHH
MAAPMAMEDTEIEIVGELVSKPYIKITLQIMHDFGIDVDNHNFERFIIKGKQTYSAPGHY
HHCCCCCCCCHHHHHHHHHCCCEEEEEEEEHHHHCCCCCCCCCEEEEEECCEECCCCCCE
LVEGDASSASYFLAAGAIAGGCIKVTGIGKKSVQGDIQFADALEAMGAKIEWGDDYIKAS
EEECCCCCCEEEEEHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCCCEEEECCHHHCCC
KGELKAIDMDMNHIPDAAMTIAVAALFATGTTKIRNVYNWRVKETDRLYAMATELRKVGA
CCCEEEEECCHHHCCHHHHHHHHHHHHHCCCHHHHHHEEEEECCCHHHHHHHHHHHHHCC
TVVEGHDFIEVTAPEKLIHAEIDTYDDHRMAMCFSLVALGNTQVTINDPKCTSKTFPDYF
EEECCCCEEEEECCHHEEEEECCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHH
EKLASVSC
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA