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The map label for this gene is ispE

Identifier: 119944673

GI number: 119944673

Start: 1141705

End: 1142559

Strand: Reverse

Name: ispE

Synonym: Ping_0912

Alternate gene names: 119944673

Gene position: 1142559-1141705 (Counterclockwise)

Preceding gene: 119944674

Following gene: 119944672

Centisome position: 25.06

GC content: 38.83

Gene sequence:

>855_bases
ATGATACATAATGAAACTATTCGTTGGCCTGCTCCTGCTAAACTGAACTTATTCTTATATATAACGGGCCAACGGGAAGA
TGGTTACCATGAATTACAAACCCTATTCCAATTTATCGATCTATGTGATTATTTAACTATCACGCCTAATCTCTCTGGAA
AAATAACGCTTACGCCAAACATCGAAGGGTTGGCATTAGAAAATAACCTAATCTATAAAGCGGCGATGATATTAAAAACC
CATACGGCAGCAAATAATGGGGCACATATTACTCTGGAAAAGAATTTACCGATGGGCGGAGGCTTAGGTGGAGGTTCATC
CGATGCAGCAACAACACTGGTTGCATTAAATCATCAATGGAACATCAATTTAACAAAAGATAAATTGGCAGAAATAGGCG
TTTTATTGGGCGCGGACGTACCTGTTTTTATCTTTGGCAAAGCCGCGATAGCAGAAGGTATAGGTGAAAAATTAACCCCG
GCTTACCCCGCAGAGCGGAGTTATCTGATTGCTGTACCCGATTGCCATATTTCAACAGCCGCCGTTTTTCAGGCAAAAAA
TCTCATTCGCAATACCAAAAAAAGAACCCATTTACAGTTAATAAATCAAAATTGGCTGAACGACTGCCAGCCCTATGTGA
AAAAAAATTATCCTAAGGTTGCCAAGGTTATAGAGTGGTTGATAGAATATGCGCCAACTCAATTGACTGGCACTGGTGCA
TGTGTTTTTAGCACATTTAACAGTATTAATGAAGCTGAAATTGTGTTACATAATACTCCTGACTGGTTAGCAGCATTGAC
CGCAAAAGGATTAAATAATTCGCCATTAAATGAATTATTAGCAACACTTAAATAA

Upstream 100 bases:

>100_bases
TATCATCTTCTGAGCTGTCTTTATAAACCATAAATCGCTACTATCTTGTCTTTAAAATAACCTCCTCAACGATAATAATT
TTCAAATAGAGAGATAACCC

Downstream 100 bases:

>100_bases
AATTTATTAATTGCATTAAGGTAATTAATAACAAATGTTAATCAGCATTATTGCTTTTTGACACACACCCCTTAGGATTA
CAGAGGTCCTCACAGTGCCA

Product: 4-diphosphocytidyl-2C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MIHNETIRWPAPAKLNLFLYITGQREDGYHELQTLFQFIDLCDYLTITPNLSGKITLTPNIEGLALENNLIYKAAMILKT
HTAANNGAHITLEKNLPMGGGLGGGSSDAATTLVALNHQWNINLTKDKLAEIGVLLGADVPVFIFGKAAIAEGIGEKLTP
AYPAERSYLIAVPDCHISTAAVFQAKNLIRNTKKRTHLQLINQNWLNDCQPYVKKNYPKVAKVIEWLIEYAPTQLTGTGA
CVFSTFNSINEAEIVLHNTPDWLAALTAKGLNNSPLNELLATLK

Sequences:

>Translated_284_residues
MIHNETIRWPAPAKLNLFLYITGQREDGYHELQTLFQFIDLCDYLTITPNLSGKITLTPNIEGLALENNLIYKAAMILKT
HTAANNGAHITLEKNLPMGGGLGGGSSDAATTLVALNHQWNINLTKDKLAEIGVLLGADVPVFIFGKAAIAEGIGEKLTP
AYPAERSYLIAVPDCHISTAAVFQAKNLIRNTKKRTHLQLINQNWLNDCQPYVKKNYPKVAKVIEWLIEYAPTQLTGTGA
CVFSTFNSINEAEIVLHNTPDWLAALTAKGLNNSPLNELLATLK
>Mature_284_residues
MIHNETIRWPAPAKLNLFLYITGQREDGYHELQTLFQFIDLCDYLTITPNLSGKITLTPNIEGLALENNLIYKAAMILKT
HTAANNGAHITLEKNLPMGGGLGGGSSDAATTLVALNHQWNINLTKDKLAEIGVLLGADVPVFIFGKAAIAEGIGEKLTP
AYPAERSYLIAVPDCHISTAAVFQAKNLIRNTKKRTHLQLINQNWLNDCQPYVKKNYPKVAKVIEWLIEYAPTQLTGTGA
CVFSTFNSINEAEIVLHNTPDWLAALTAKGLNNSPLNELLATLK

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=280, Percent_Identity=52.8571428571429, Blast_Score=275, Evalue=3e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_PSYIN (A1STD9)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_942353.1
- ProteinModelPortal:   A1STD9
- SMR:   A1STD9
- STRING:   A1STD9
- GeneID:   4623567
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_0912
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   LNLFLHV
- PhylomeDB:   A1STD9
- BioCyc:   PING357804:PING_0912-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 31143; Mature: 31143

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: NA

Important sites: ACT_SITE 14-14 ACT_SITE 139-139

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHNETIRWPAPAKLNLFLYITGQREDGYHELQTLFQFIDLCDYLTITPNLSGKITLTPN
CCCCCEECCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHCCEEECCCCCEEEEECCC
IEGLALENNLIYKAAMILKTHTAANNGAHITLEKNLPMGGGLGGGSSDAATTLVALNHQW
CCEEEECCCEEEEEEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEECCEE
NINLTKDKLAEIGVLLGADVPVFIFGKAAIAEGIGEKLTPAYPAERSYLIAVPDCHISTA
EEEECHHHHHHHHHHCCCCCCEEEECHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHH
AVFQAKNLIRNTKKRTHLQLINQNWLNDCQPYVKKNYPKVAKVIEWLIEYAPTQLTGTGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCH
CVFSTFNSINEAEIVLHNTPDWLAALTAKGLNNSPLNELLATLK
HEEHHHCCCCCEEEEEECCCHHHHHHHHCCCCCCCHHHHHHHCC
>Mature Secondary Structure
MIHNETIRWPAPAKLNLFLYITGQREDGYHELQTLFQFIDLCDYLTITPNLSGKITLTPN
CCCCCEECCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHCCEEECCCCCEEEEECCC
IEGLALENNLIYKAAMILKTHTAANNGAHITLEKNLPMGGGLGGGSSDAATTLVALNHQW
CCEEEECCCEEEEEEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEEEECCEE
NINLTKDKLAEIGVLLGADVPVFIFGKAAIAEGIGEKLTPAYPAERSYLIAVPDCHISTA
EEEECHHHHHHHHHHCCCCCCEEEECHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHH
AVFQAKNLIRNTKKRTHLQLINQNWLNDCQPYVKKNYPKVAKVIEWLIEYAPTQLTGTGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCH
CVFSTFNSINEAEIVLHNTPDWLAALTAKGLNNSPLNELLATLK
HEEHHHCCCCCEEEEEECCCHHHHHHHHCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA