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The map label for this gene is rfbS [H]

Identifier: 119944542

GI number: 119944542

Start: 975737

End: 976582

Strand: Direct

Name: rfbS [H]

Synonym: Ping_0776

Alternate gene names: 119944542

Gene position: 975737-976582 (Clockwise)

Preceding gene: 119944541

Following gene: 119944543

Centisome position: 21.4

GC content: 37.12

Gene sequence:

>846_bases
ATGGTTATATTTATTTCTGGTGCTTCGGGTTATTTAGGTAGTCACTTAGTTAAAGCATTATCAAAAGAAAATCGAGTTTT
CGCACTTATTCGTAGGTCATCATCAAGGGCACGCGTTGAAGGTGTTGATGTTATTTATATTGATGAAGGTGATGTACTAG
AAAAAGCTTTTGCACTGCATCAGCCCGGTGTAATTATCAACACCGCGGCGCTCTATGGTCGAAAAGGGGAATCTTTATCT
GCCTTGGTTAATGCAAACATTGATTTTCCAACTCAATTATTAGCTTTAGCAGATAAATATAAGAGTAAAGCATTTATTCA
TACTGGTACTTCTTTACCGGATGACATTTCACCTTATGCATTAACAAAAAACACCTTTGTTAAGCTTGCCCAATTCAATG
TAGAAAGTCCTCTTAAATTTGTGAATGTTGCTTTAGAGCATTTTTATGGCCCTGAAGATGACAGTAGTAAATTTACTTCG
TATGTTATCAATGCATGCCTTGCCGGTAACAAACTTGCTTTAACGAATGGCCTCCAACAACGTGATTTCATTTATATTAA
TGATGTTGTTGATGCGTATAAAGTGCTTATTGAAAATATAGACAAATTAGACTCTTTCGAGACAGTACCAGTTGGCTCCG
GTCTAGCACCTAGCGTTAGAGAGTTAGTTGAAATGATCCATTCTTGTAGTCATTCAAAATCAATGCTTGATTTCGGAGCA
GTAGCTATGAGAGTAAATGAGCTGATGTACAGTTGCGCCGATACAAGTAGATTAAAGCAATTAGGCTGGCAACCTGCATA
CTCTTTAGAGTCAGGCATTCAGGCCACATTAAAAGGAAATGTATAA

Upstream 100 bases:

>100_bases
ACAGATCATTTAGACTTTGTTATTGAAAAATTTGAAGAGTTTCTTGGTTTGAACTTCTGAAAAGGGTGGGAGGCTTTGGC
CTCCAAATGAGGATCTGTTT

Downstream 100 bases:

>100_bases
TGAAGTTATTAATCACTGGCGGATGTGGTTTTTTAGGCAGTAATTTAGCCTCTCATGGTATCAAAAAAGGCTATGAAGTG
ACGGTTTTTGATAATTTATC

Product: UDP-glucose 4-epimerase

Products: GDP-4-dehydro-6-L-deoxygalactose; guanosine diphosphate fucose; NADP [C]

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MVIFISGASGYLGSHLVKALSKENRVFALIRRSSSRARVEGVDVIYIDEGDVLEKAFALHQPGVIINTAALYGRKGESLS
ALVNANIDFPTQLLALADKYKSKAFIHTGTSLPDDISPYALTKNTFVKLAQFNVESPLKFVNVALEHFYGPEDDSSKFTS
YVINACLAGNKLALTNGLQQRDFIYINDVVDAYKVLIENIDKLDSFETVPVGSGLAPSVRELVEMIHSCSHSKSMLDFGA
VAMRVNELMYSCADTSRLKQLGWQPAYSLESGIQATLKGNV

Sequences:

>Translated_281_residues
MVIFISGASGYLGSHLVKALSKENRVFALIRRSSSRARVEGVDVIYIDEGDVLEKAFALHQPGVIINTAALYGRKGESLS
ALVNANIDFPTQLLALADKYKSKAFIHTGTSLPDDISPYALTKNTFVKLAQFNVESPLKFVNVALEHFYGPEDDSSKFTS
YVINACLAGNKLALTNGLQQRDFIYINDVVDAYKVLIENIDKLDSFETVPVGSGLAPSVRELVEMIHSCSHSKSMLDFGA
VAMRVNELMYSCADTSRLKQLGWQPAYSLESGIQATLKGNV
>Mature_281_residues
MVIFISGASGYLGSHLVKALSKENRVFALIRRSSSRARVEGVDVIYIDEGDVLEKAFALHQPGVIINTAALYGRKGESLS
ALVNANIDFPTQLLALADKYKSKAFIHTGTSLPDDISPYALTKNTFVKLAQFNVESPLKFVNVALEHFYGPEDDSSKFTS
YVINACLAGNKLALTNGLQQRDFIYINDVVDAYKVLIENIDKLDSFETVPVGSGLAPSVRELVEMIHSCSHSKSMLDFGA
VAMRVNELMYSCADTSRLKQLGWQPAYSLESGIQATLKGNV

Specific function: Two Step Nadp-Dependent Conversion Of Gdp-4-Dehydro-6- Deoxy-D-Mannose To Gdp-Fucose, Involving An Epimerase And A Reductase Reaction. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 1.1.1.271 [C]

Molecular weight: Translated: 30736; Mature: 30736

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVIFISGASGYLGSHLVKALSKENRVFALIRRSSSRARVEGVDVIYIDEGDVLEKAFALH
CEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCEECCEEEEEECCCHHHHHHHHHC
QPGVIINTAALYGRKGESLSALVNANIDFPTQLLALADKYKSKAFIHTGTSLPDDISPYA
CCCEEEEHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCE
LTKNTFVKLAQFNVESPLKFVNVALEHFYGPEDDSSKFTSYVINACLAGNKLALTNGLQQ
ECHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCC
RDFIYINDVVDAYKVLIENIDKLDSFETVPVGSGLAPSVRELVEMIHSCSHSKSMLDFGA
CCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHH
VAMRVNELMYSCADTSRLKQLGWQPAYSLESGIQATLKGNV
HHHHHHHHHHHHCCHHHHHHCCCCCCHHHHCCCEEEECCCC
>Mature Secondary Structure
MVIFISGASGYLGSHLVKALSKENRVFALIRRSSSRARVEGVDVIYIDEGDVLEKAFALH
CEEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCEECCEEEEEECCCHHHHHHHHHC
QPGVIINTAALYGRKGESLSALVNANIDFPTQLLALADKYKSKAFIHTGTSLPDDISPYA
CCCEEEEHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCCCCE
LTKNTFVKLAQFNVESPLKFVNVALEHFYGPEDDSSKFTSYVINACLAGNKLALTNGLQQ
ECHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCC
RDFIYINDVVDAYKVLIENIDKLDSFETVPVGSGLAPSVRELVEMIHSCSHSKSMLDFGA
CCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHH
VAMRVNELMYSCADTSRLKQLGWQPAYSLESGIQATLKGNV
HHHHHHHHHHHHCCHHHHHHCCCCCCHHHHCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDP-4-dehydro-6-deoxy-D-mannose; GDP-4-dehydro-6-L-deoxygalactose; NADPH; Proton [C]

Specific reaction: GDP-4-dehydro-6-deoxy-D-mannose = GDP-4-dehydro-6-L-deoxygalactose GDP-4-dehydro-6-L-deoxygalactose + NADPH + Proton = guanosine diphosphate fucose + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2793833; 11677608; 12644504 [H]