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The map label for this gene is eno

Identifier: 119944441

GI number: 119944441

Start: 857814

End: 859121

Strand: Direct

Name: eno

Synonym: Ping_0669

Alternate gene names: 119944441

Gene position: 857814-859121 (Clockwise)

Preceding gene: 119944440

Following gene: 119944442

Centisome position: 18.81

GC content: 42.51

Gene sequence:

>1308_bases
ATGTCCACTATCGTAAAAGTACTTGGTCGCGAAATCATGGATTCACGTGGTAACCCAACTGTTGAAGCTGAAGTTCACTT
AGCTGACGGTTCAATCGGTATGGCTGCAGCACCTTCTGGCGCATCTACCGGTTCTCGTGAAGCATTAGAACTGCGTGACG
GTGATAAAGCGCGTTACTTAGGTAAAGGCGTGCTTAAAGCTGTTACTGCTGTTAACGGCCCAATCGCTGAAGCGCTTATT
GGTAAAGATGCAGTTAAACAAGCTGAACTTGATCAAATCATGATTGATTTAGATGGTACTGAAAACAAAGCTAAATTTGG
CGCAAATGCTATTTTGGCTGTTTCACTGGCGGCAGCAAAAGCGGCTGCAGTATCGAAAAAAGTGCCTCTGTACGCGCATA
TCGCTGACCTTAACGGTACACCCGGTGTTTACTCTATGCCGCTTCCAATGATGAATATCATCAATGGTGGTGAGCATGCT
GATAACTCTGTTGATATCCAAGAGTTCATGATTCAACCTGTTGGCGCATCTACATTCCGTGAAGGTCTGCGTATGGGCGC
TGAAGTATTCCATAGCCTTGCTAAAGTACTTAAAGCTGATGGCCACTCAACGGCTGTTGGTGATGAAGGTGGTTTTGCTC
CAAACCTTGAGTCTAACGCTGCTGCACTTGCTGCCATTAAAGTGGCTGTTGCAAACGCAGGTTACGAACTAGGTAAAGAC
ATCACTCTAGCAATGGATTGTGCTGCATCTGAGTTCTATAACAAAGAAACTGGCATGTACGAACTTAAAGGTGAAGGTAA
GACTTTTACTGCTAAAGAGTTCAACTACTTCCTTGAAGATCTTGTTAACCAATACCCAATTGTTTCTATCGAAGATGGTC
TTGACGAGTCTGATTGGGATGGTTTCAAACACCAAACTGAACTACTAGGTGATAAAATTCAACTAGTCGGTGACGATTTG
TTTGTCACAAACACTAAGATTCTGGCTCGTGGTATCAAAGAAGGTATTACTAACTCTATCTTAATCAAATTCAACCAAAT
CGGTTCTTTAACTGAAACTCTTGCTGCAATCAAAATGGCTAAAGATGCTGGCTTTACTGCTGTTATCTCTCACCGTTCTG
GTGAAACAGAAGATTCAACTATTGCAGACCTTGCTGTTGGTACTGCTGCTGGCCAAATCAAAACGGGTTCATTAAGCCGT
AGTGATCGTGTTGCTAAATATAACCAGCTTCTACGTATTGAAGAAGCGCTAGGTTCAAAAGCACCTTACAATGGTCTTAA
AGAAGTTAAAGGTACTTTTAACTTTTAA

Upstream 100 bases:

>100_bases
ACCAGATCACTTTTTTTTGTTAAAAAATAACGCATTAAAGTGATCTGGAATTATATAACCCATTTTTAAACTTATTTTTA
ATACAACTAAAGGAACAATT

Downstream 100 bases:

>100_bases
TTTAAACTAATTCAGTTTGAATCATAATCCTCACTCCGGTGGGGATTTTTTTTGCCTGAAGAAAAGCTGTCGAGCTGTCG
GCTGTCCGTTGATGGCTGTC

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MSTIVKVLGREIMDSRGNPTVEAEVHLADGSIGMAAAPSGASTGSREALELRDGDKARYLGKGVLKAVTAVNGPIAEALI
GKDAVKQAELDQIMIDLDGTENKAKFGANAILAVSLAAAKAAAVSKKVPLYAHIADLNGTPGVYSMPLPMMNIINGGEHA
DNSVDIQEFMIQPVGASTFREGLRMGAEVFHSLAKVLKADGHSTAVGDEGGFAPNLESNAAALAAIKVAVANAGYELGKD
ITLAMDCAASEFYNKETGMYELKGEGKTFTAKEFNYFLEDLVNQYPIVSIEDGLDESDWDGFKHQTELLGDKIQLVGDDL
FVTNTKILARGIKEGITNSILIKFNQIGSLTETLAAIKMAKDAGFTAVISHRSGETEDSTIADLAVGTAAGQIKTGSLSR
SDRVAKYNQLLRIEEALGSKAPYNGLKEVKGTFNF

Sequences:

>Translated_435_residues
MSTIVKVLGREIMDSRGNPTVEAEVHLADGSIGMAAAPSGASTGSREALELRDGDKARYLGKGVLKAVTAVNGPIAEALI
GKDAVKQAELDQIMIDLDGTENKAKFGANAILAVSLAAAKAAAVSKKVPLYAHIADLNGTPGVYSMPLPMMNIINGGEHA
DNSVDIQEFMIQPVGASTFREGLRMGAEVFHSLAKVLKADGHSTAVGDEGGFAPNLESNAAALAAIKVAVANAGYELGKD
ITLAMDCAASEFYNKETGMYELKGEGKTFTAKEFNYFLEDLVNQYPIVSIEDGLDESDWDGFKHQTELLGDKIQLVGDDL
FVTNTKILARGIKEGITNSILIKFNQIGSLTETLAAIKMAKDAGFTAVISHRSGETEDSTIADLAVGTAAGQIKTGSLSR
SDRVAKYNQLLRIEEALGSKAPYNGLKEVKGTFNF
>Mature_434_residues
STIVKVLGREIMDSRGNPTVEAEVHLADGSIGMAAAPSGASTGSREALELRDGDKARYLGKGVLKAVTAVNGPIAEALIG
KDAVKQAELDQIMIDLDGTENKAKFGANAILAVSLAAAKAAAVSKKVPLYAHIADLNGTPGVYSMPLPMMNIINGGEHAD
NSVDIQEFMIQPVGASTFREGLRMGAEVFHSLAKVLKADGHSTAVGDEGGFAPNLESNAAALAAIKVAVANAGYELGKDI
TLAMDCAASEFYNKETGMYELKGEGKTFTAKEFNYFLEDLVNQYPIVSIEDGLDESDWDGFKHQTELLGDKIQLVGDDLF
VTNTKILARGIKEGITNSILIKFNQIGSLTETLAAIKMAKDAGFTAVISHRSGETEDSTIADLAVGTAAGQIKTGSLSRS
DRVAKYNQLLRIEEALGSKAPYNGLKEVKGTFNF

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI301897477, Length=431, Percent_Identity=53.5962877030162, Blast_Score=433, Evalue=1e-121,
Organism=Homo sapiens, GI301897469, Length=431, Percent_Identity=53.5962877030162, Blast_Score=433, Evalue=1e-121,
Organism=Homo sapiens, GI5803011, Length=432, Percent_Identity=53.2407407407407, Blast_Score=430, Evalue=1e-120,
Organism=Homo sapiens, GI4503571, Length=432, Percent_Identity=52.3148148148148, Blast_Score=424, Evalue=1e-118,
Organism=Homo sapiens, GI301897479, Length=429, Percent_Identity=48.951048951049, Blast_Score=377, Evalue=1e-104,
Organism=Homo sapiens, GI169201331, Length=357, Percent_Identity=30.5322128851541, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI169201757, Length=357, Percent_Identity=30.5322128851541, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI239744207, Length=357, Percent_Identity=30.5322128851541, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI310129182, Length=227, Percent_Identity=26.431718061674, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI310110045, Length=227, Percent_Identity=26.431718061674, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI310120572, Length=227, Percent_Identity=26.431718061674, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1789141, Length=432, Percent_Identity=81.712962962963, Blast_Score=696, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17536383, Length=433, Percent_Identity=52.6558891454965, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI71995829, Length=433, Percent_Identity=52.6558891454965, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI32563855, Length=230, Percent_Identity=43.9130434782609, Blast_Score=172, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6321693, Length=433, Percent_Identity=48.2678983833718, Blast_Score=365, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6323985, Length=437, Percent_Identity=45.9954233409611, Blast_Score=359, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6324974, Length=437, Percent_Identity=45.766590389016, Blast_Score=357, Evalue=1e-99,
Organism=Saccharomyces cerevisiae, GI6324969, Length=437, Percent_Identity=45.766590389016, Blast_Score=357, Evalue=1e-99,
Organism=Saccharomyces cerevisiae, GI6321968, Length=433, Percent_Identity=47.3441108545035, Blast_Score=341, Evalue=1e-94,
Organism=Drosophila melanogaster, GI24580918, Length=433, Percent_Identity=51.7321016166282, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580916, Length=433, Percent_Identity=51.7321016166282, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580920, Length=433, Percent_Identity=51.7321016166282, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580914, Length=433, Percent_Identity=51.7321016166282, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI281360527, Length=433, Percent_Identity=51.7321016166282, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI17137654, Length=433, Percent_Identity=51.7321016166282, Blast_Score=388, Evalue=1e-108,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_PSYIN (A1SSQ7)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_942121.1
- ProteinModelPortal:   A1SSQ7
- SMR:   A1SSQ7
- STRING:   A1SSQ7
- GeneID:   4626906
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_0669
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- PhylomeDB:   A1SSQ7
- ProtClustDB:   PRK00077
- BioCyc:   PING357804:PING_0669-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 45966; Mature: 45834

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 209-209 ACT_SITE 343-343 BINDING 159-159 BINDING 168-168 BINDING 291-291 BINDING 318-318 BINDING 343-343 BINDING 394-394

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIVKVLGREIMDSRGNPTVEAEVHLADGSIGMAAAPSGASTGSREALELRDGDKARYL
CHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCEECCCCCCCCCCCEEEECCCCHHHHH
GKGVLKAVTAVNGPIAEALIGKDAVKQAELDQIMIDLDGTENKAKFGANAILAVSLAAAK
HHHHHHHHHHCCCHHHHHHHCHHHHHHHCCCEEEEEECCCCCCHHHCCCCEEEHHHHHHH
AAAVSKKVPLYAHIADLNGTPGVYSMPLPMMNIINGGEHADNSVDIQEFMIQPVGASTFR
HHHHHCCCCEEEEEECCCCCCCEEECCCHHHHHHCCCCCCCCCCCHHHHHHCCCCHHHHH
EGLRMGAEVFHSLAKVLKADGHSTAVGDEGGFAPNLESNAAALAAIKVAVANAGYELGKD
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHCCC
ITLAMDCAASEFYNKETGMYELKGEGKTFTAKEFNYFLEDLVNQYPIVSIEDGLDESDWD
EEEEEHHHHHHHHCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHCCEEEECCCCCCCCCC
GFKHQTELLGDKIQLVGDDLFVTNTKILARGIKEGITNSILIKFNQIGSLTETLAAIKMA
CHHHHHHHHCCCEEEECCCEEEECHHHHHHHHHHCCCCEEEEEEHHHCHHHHHHHHHHHH
KDAGFTAVISHRSGETEDSTIADLAVGTAAGQIKTGSLSRSDRVAKYNQLLRIEEALGSK
HCCCCEEEEECCCCCCCCCHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCC
APYNGLKEVKGTFNF
CCCCCHHHHCCCCCC
>Mature Secondary Structure 
STIVKVLGREIMDSRGNPTVEAEVHLADGSIGMAAAPSGASTGSREALELRDGDKARYL
HHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCEECCCCCCCCCCCEEEECCCCHHHHH
GKGVLKAVTAVNGPIAEALIGKDAVKQAELDQIMIDLDGTENKAKFGANAILAVSLAAAK
HHHHHHHHHHCCCHHHHHHHCHHHHHHHCCCEEEEEECCCCCCHHHCCCCEEEHHHHHHH
AAAVSKKVPLYAHIADLNGTPGVYSMPLPMMNIINGGEHADNSVDIQEFMIQPVGASTFR
HHHHHCCCCEEEEEECCCCCCCEEECCCHHHHHHCCCCCCCCCCCHHHHHHCCCCHHHHH
EGLRMGAEVFHSLAKVLKADGHSTAVGDEGGFAPNLESNAAALAAIKVAVANAGYELGKD
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHCCC
ITLAMDCAASEFYNKETGMYELKGEGKTFTAKEFNYFLEDLVNQYPIVSIEDGLDESDWD
EEEEEHHHHHHHHCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHCCEEEECCCCCCCCCC
GFKHQTELLGDKIQLVGDDLFVTNTKILARGIKEGITNSILIKFNQIGSLTETLAAIKMA
CHHHHHHHHCCCEEEECCCEEEECHHHHHHHHHHCCCCEEEEEEHHHCHHHHHHHHHHHH
KDAGFTAVISHRSGETEDSTIADLAVGTAAGQIKTGSLSRSDRVAKYNQLLRIEEALGSK
HCCCCEEEEECCCCCCCCCHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCC
APYNGLKEVKGTFNF
CCCCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA