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The map label for this gene is panB

Identifier: 119944353

GI number: 119944353

Start: 759294

End: 760088

Strand: Reverse

Name: panB

Synonym: Ping_0580

Alternate gene names: 119944353

Gene position: 760088-759294 (Counterclockwise)

Preceding gene: 119944354

Following gene: 119944352

Centisome position: 16.67

GC content: 43.65

Gene sequence:

>795_bases
ATGGCTAAAATCAGCGTTTCTCGTCTGACTAAAATGAAACAAGACAATCAAAAAATCACCTGCATGACCGCATATGATGC
GAGTTTTGCAGCTATTTTTGACCAGGCCGGTATCCAAGTATTATTAGTAGGTGACTCACTCGGCATGGTATTACAGGGAC
ATGATTCTACGCTGCCAGTGACAGTTGACGATATCAGATACCATACAGCTGCGGTTGTAAGTACCGCTAAAAGTGCATTC
ATTATCGCTGATTTACCCTTTATGAGTTATTCAACGCCAGAAATGAGCTATGAAAATGCCGCAATATTAATGCGTGCAGG
CGCTAATATGGTAAAAATGGAGGGCGGCGAATGGCTCACTGATTCCGTAAAAGGATTAGTGGAACGTGGTATTCCAGTCT
GTGGTCACCTTGGTTTAACACCACAATCGGTCAATATTTTTGGTGGTTACAAAGTACAAGGACGCGAGGAAGAACAGGCA
GACGAGCTGCTCAACGACGCATTATTGCTCCAGGAAGCAGGGATACAACTGTTAGTTCTAGAATGCGTTCCGGTCAGTTT
AGCCGATCGCATTACGACAGCCTTAAAAATTCCAGTGATTGGGATTGGAGCAGGGTGTGAAACCGACGGACAAATTTTAG
TGATGCATGATGCCTTTGGTGTCTCGGCTGGTTTTTGTCCAAAATTTTCCAAAAACTTCCTGCTTGAAACAGGCGATATT
CGCCAGGCGGTAACGCTTTATATAGATCAAGTTGAAAGCAGACAATTCCCGTCTGCTGAACACAGTTTTTATTAA

Upstream 100 bases:

>100_bases
ATACAAAAAGTTGCCGTCATTAATTTTTTATCTCAGATTTTTCCTACTCTGAATTAATTAATGTGACAGGTATAAATGTA
ATTTTAATTTTGGAGTAATC

Downstream 100 bases:

>100_bases
GTATATTGGCAGATAAAAACATGCTTATTATTGATAATCCACAGCAACTACGTGAAAAGATAAAACAACTCAAACAGCAA
GGGTCTGAAATTGCTTTTGT

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MAKISVSRLTKMKQDNQKITCMTAYDASFAAIFDQAGIQVLLVGDSLGMVLQGHDSTLPVTVDDIRYHTAAVVSTAKSAF
IIADLPFMSYSTPEMSYENAAILMRAGANMVKMEGGEWLTDSVKGLVERGIPVCGHLGLTPQSVNIFGGYKVQGREEEQA
DELLNDALLLQEAGIQLLVLECVPVSLADRITTALKIPVIGIGAGCETDGQILVMHDAFGVSAGFCPKFSKNFLLETGDI
RQAVTLYIDQVESRQFPSAEHSFY

Sequences:

>Translated_264_residues
MAKISVSRLTKMKQDNQKITCMTAYDASFAAIFDQAGIQVLLVGDSLGMVLQGHDSTLPVTVDDIRYHTAAVVSTAKSAF
IIADLPFMSYSTPEMSYENAAILMRAGANMVKMEGGEWLTDSVKGLVERGIPVCGHLGLTPQSVNIFGGYKVQGREEEQA
DELLNDALLLQEAGIQLLVLECVPVSLADRITTALKIPVIGIGAGCETDGQILVMHDAFGVSAGFCPKFSKNFLLETGDI
RQAVTLYIDQVESRQFPSAEHSFY
>Mature_263_residues
AKISVSRLTKMKQDNQKITCMTAYDASFAAIFDQAGIQVLLVGDSLGMVLQGHDSTLPVTVDDIRYHTAAVVSTAKSAFI
IADLPFMSYSTPEMSYENAAILMRAGANMVKMEGGEWLTDSVKGLVERGIPVCGHLGLTPQSVNIFGGYKVQGREEEQAD
ELLNDALLLQEAGIQLLVLECVPVSLADRITTALKIPVIGIGAGCETDGQILVMHDAFGVSAGFCPKFSKNFLLETGDIR
QAVTLYIDQVESRQFPSAEHSFY

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=264, Percent_Identity=66.2878787878788, Blast_Score=369, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6319653, Length=251, Percent_Identity=39.4422310756972, Blast_Score=169, Evalue=3e-43,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_PSYIN (A1SSG9)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_942033.1
- ProteinModelPortal:   A1SSG9
- SMR:   A1SSG9
- STRING:   A1SSG9
- GeneID:   4624135
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_0580
- eggNOG:   COG0413
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- PhylomeDB:   A1SSG9
- BioCyc:   PING357804:PING_0580-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 28545; Mature: 28414

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: ACT_SITE 181-181 BINDING 84-84 BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKISVSRLTKMKQDNQKITCMTAYDASFAAIFDQAGIQVLLVGDSLGMVLQGHDSTLPV
CCCCCHHHHHHHHCCCCEEEEEEECCCHHHHHHHCCCEEEEEEECCCCEEEECCCCCCCE
TVDDIRYHTAAVVSTAKSAFIIADLPFMSYSTPEMSYENAAILMRAGANMVKMEGGEWLT
EEHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCHH
DSVKGLVERGIPVCGHLGLTPQSVNIFGGYKVQGREEEQADELLNDALLLQEAGIQLLVL
HHHHHHHHCCCCEEECCCCCCCEEEEEECEEECCCCHHHHHHHHHHHHHHHHCCCEEEEE
ECVPVSLADRITTALKIPVIGIGAGCETDGQILVMHDAFGVSAGFCPKFSKNFLLETGDI
EECCCHHHHHHHHEEEEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEECCCH
RQAVTLYIDQVESRQFPSAEHSFY
HHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
AKISVSRLTKMKQDNQKITCMTAYDASFAAIFDQAGIQVLLVGDSLGMVLQGHDSTLPV
CCCCHHHHHHHHCCCCEEEEEEECCCHHHHHHHCCCEEEEEEECCCCEEEECCCCCCCE
TVDDIRYHTAAVVSTAKSAFIIADLPFMSYSTPEMSYENAAILMRAGANMVKMEGGEWLT
EEHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCHH
DSVKGLVERGIPVCGHLGLTPQSVNIFGGYKVQGREEEQADELLNDALLLQEAGIQLLVL
HHHHHHHHCCCCEEECCCCCCCEEEEEECEEECCCCHHHHHHHHHHHHHHHHCCCEEEEE
ECVPVSLADRITTALKIPVIGIGAGCETDGQILVMHDAFGVSAGFCPKFSKNFLLETGDI
EECCCHHHHHHHHEEEEEEEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEECCCH
RQAVTLYIDQVESRQFPSAEHSFY
HHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA