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The map label for this gene is thiG [H]

Identifier: 119944145

GI number: 119944145

Start: 467169

End: 467966

Strand: Direct

Name: thiG [H]

Synonym: Ping_0364

Alternate gene names: 119944145

Gene position: 467169-467966 (Clockwise)

Preceding gene: 119944144

Following gene: 119944146

Centisome position: 10.25

GC content: 45.61

Gene sequence:

>798_bases
ATGTTATTTAATAAAATGCAGGGCAATGATCCATTAGTGATTGCCGATAAAACCTTCACTTCGCGATTATTTACTGGCAC
AGGTAAATTTAATGACCAAAAAACTATGATTGCAGCGCTCAGGGCATCCCAATCTGAATTAGTCACTATGGCGTTAAAGC
GTATTGATATGGAGCATAAGGATGATGATATTCTCGCGCCCTTAATTAAAAACGGGATGAATTTATTACCTAATACTTCC
GGTGCCCGTAATGCCAAAGAGGCGATATTTGCTGCACAACTCGCCCGTGAAGCCTTGCAGACCAACTGGATAAAATTAGA
AATTCATCCGGATCCTAAGTATCTGCTGCCGGATCCTATTGAAACATTAAAAGCCGCTGAATTTTTAGCGCGTGATGGTT
TTGTGGTACTGCCCTATGTGCATGCCGATCCTGTTTTGTGTAAACATTTAGAAGCCGCCGGCTGTGCGGCGGTAATGCCA
TTGGGCGCCCCCATTGGTTCTAATAAAGGGTTAAAAACCATTGATTTTTTAGAGATTATTATTGAGCAGGCGAATATTCC
GGTGATTGTGGATGCAGGCATTGGCAGTCCTTCGCATGCGGCGCTGGCAATGGAGTTAGGTGCCGATGCAGTGTTAGTGA
ATACCGCCATGGCGGTGGCAAATGATCCCGTTTTGATGAGTGAAGCTTTTGCTAAAGCGGTGCAGGCCGGGCGACAGGCA
TATCTTGCAGGTTTAGCGGGACAGGTGAATCATGCTGTTGCGTCAAGTCCACTGACTGCCTTTTTACAGGCGGGGTAA

Upstream 100 bases:

>100_bases
TAATCAGCATATTATAAGCCGTTCAAAATGGGCTGAATATAAACTTAATGAAGGCGATCAAATTTCGCTTTTTCAAGCCA
TAGCAGGAGGCTAGCCATCA

Downstream 100 bases:

>100_bases
GCAAATTATGAGCTTTAGTGAACAGATAAAAAATTATCAGTGGGATGATATCCGCCTGTCTATTTATGGTAAAAGTGAAA
ATGATGTAAAGCGCGCTTTG

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MLFNKMQGNDPLVIADKTFTSRLFTGTGKFNDQKTMIAALRASQSELVTMALKRIDMEHKDDDILAPLIKNGMNLLPNTS
GARNAKEAIFAAQLAREALQTNWIKLEIHPDPKYLLPDPIETLKAAEFLARDGFVVLPYVHADPVLCKHLEAAGCAAVMP
LGAPIGSNKGLKTIDFLEIIIEQANIPVIVDAGIGSPSHAALAMELGADAVLVNTAMAVANDPVLMSEAFAKAVQAGRQA
YLAGLAGQVNHAVASSPLTAFLQAG

Sequences:

>Translated_265_residues
MLFNKMQGNDPLVIADKTFTSRLFTGTGKFNDQKTMIAALRASQSELVTMALKRIDMEHKDDDILAPLIKNGMNLLPNTS
GARNAKEAIFAAQLAREALQTNWIKLEIHPDPKYLLPDPIETLKAAEFLARDGFVVLPYVHADPVLCKHLEAAGCAAVMP
LGAPIGSNKGLKTIDFLEIIIEQANIPVIVDAGIGSPSHAALAMELGADAVLVNTAMAVANDPVLMSEAFAKAVQAGRQA
YLAGLAGQVNHAVASSPLTAFLQAG
>Mature_265_residues
MLFNKMQGNDPLVIADKTFTSRLFTGTGKFNDQKTMIAALRASQSELVTMALKRIDMEHKDDDILAPLIKNGMNLLPNTS
GARNAKEAIFAAQLAREALQTNWIKLEIHPDPKYLLPDPIETLKAAEFLARDGFVVLPYVHADPVLCKHLEAAGCAAVMP
LGAPIGSNKGLKTIDFLEIIIEQANIPVIVDAGIGSPSHAALAMELGADAVLVNTAMAVANDPVLMSEAFAKAVQAGRQA
YLAGLAGQVNHAVASSPLTAFLQAG

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family [H]

Homologues:

Organism=Escherichia coli, GI48994993, Length=253, Percent_Identity=67.5889328063241, Blast_Score=341, Evalue=3e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR008867 [H]

Pfam domain/function: PF05690 ThiG [H]

EC number: NA

Molecular weight: Translated: 28148; Mature: 28148

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFNKMQGNDPLVIADKTFTSRLFTGTGKFNDQKTMIAALRASQSELVTMALKRIDMEHK
CCCCCCCCCCCEEEECCCHHHHEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCC
DDDILAPLIKNGMNLLPNTSGARNAKEAIFAAQLAREALQTNWIKLEIHPDPKYLLPDPI
CCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHH
ETLKAAEFLARDGFVVLPYVHADPVLCKHLEAAGCAAVMPLGAPIGSNKGLKTIDFLEII
HHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHH
IEQANIPVIVDAGIGSPSHAALAMELGADAVLVNTAMAVANDPVLMSEAFAKAVQAGRQA
HHCCCCCEEEECCCCCCCHHEEEEECCCCEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHH
YLAGLAGQVNHAVASSPLTAFLQAG
HHHHHHHHHHHHHHCCCHHHHEECC
>Mature Secondary Structure
MLFNKMQGNDPLVIADKTFTSRLFTGTGKFNDQKTMIAALRASQSELVTMALKRIDMEHK
CCCCCCCCCCCEEEECCCHHHHEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCC
DDDILAPLIKNGMNLLPNTSGARNAKEAIFAAQLAREALQTNWIKLEIHPDPKYLLPDPI
CCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHH
ETLKAAEFLARDGFVVLPYVHADPVLCKHLEAAGCAAVMPLGAPIGSNKGLKTIDFLEII
HHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHH
IEQANIPVIVDAGIGSPSHAALAMELGADAVLVNTAMAVANDPVLMSEAFAKAVQAGRQA
HHCCCCCEEEECCCCCCCHHEEEEECCCCEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHH
YLAGLAGQVNHAVASSPLTAFLQAG
HHHHHHHHHHHHHHCCCHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA