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The map label for this gene is thiH [H]

Identifier: 119944146

GI number: 119944146

Start: 467974

End: 469092

Strand: Direct

Name: thiH [H]

Synonym: Ping_0365

Alternate gene names: 119944146

Gene position: 467974-469092 (Clockwise)

Preceding gene: 119944145

Following gene: 119944147

Centisome position: 10.26

GC content: 40.39

Gene sequence:

>1119_bases
ATGAGCTTTAGTGAACAGATAAAAAATTATCAGTGGGATGATATCCGCCTGTCTATTTATGGTAAAAGTGAAAATGATGT
AAAGCGCGCTTTGTCTAAAGAACGTTTAGATTTAGAAGACTTTAAAGCGTTAATCTCTCCCGCAGCTGAGCCTTTCCTCG
AGCAAATGGCACAAAAATCACAACAACTGACCCAGCAGCGTTTTGGTAAAACACAGCAATTTTTTATTCCCCTCTATTTG
TCCAATATGTGCAGCAATATCTGCACCTATTGTGGTTTTTCTATGCATAATGCTATTCGTCGCAAGACCTTGGATATGAA
AGAGATTGAAGATGAGTGTTTAGCGATTAAAAAAATGGGTTTTGCCCATATTTTACTGGTGACCGGGGAGTCTGAACGCA
AGGTTGGGGTTGAGTATTTTAAACAGGCGCTGCCTATTATAAAAAAACATTTTTCGCATATCTCCATTGAGGTGCAGCCT
TTAGATCAGCACGAATATGAAGCGCTTATTGAGTATGGTGTTGATGCCGTATTGGTGTATCAGGAAACCTATAATCCGGT
TACCTATGCAGAGCATCATTTAAAGGGTAAAAAATCTGATTTTAAGTACCGTTTAGATACCCATGATAGACTCGGCAAAG
CGGGTATGCTTAAAATGGGCTTAGGCTGTTTAATTGGTCTTGAGGAATGGCGCACCGATTGTTTTTATGTGGCCGCACAT
CTGAATTATTTAGAGAAAATATACTGGCAAAGCCGCTATGCAATCTCATTTCCACGTCTACGTCCCTGTGCAGGCGGCAT
GGAAATAAAGTCGGTGATGGATGATAAAGAACTGGTACAATTGATTTGTGCCTATCGATTATTTAGTCCCGAGGTTGAAT
TATCACTTTCAACACGCGAATCTGAACATTTTAGAGATCATGTTTTACCTTTAGGTATTACATCATTAAGTGCGGGATCC
AGTACACAGCCCGGTGGTTATGCAGCAACATCGACAAAAGCCCTTGAGCAGTTTGAAATATCCGATGACAGATCCCCGGC
TAAAATGGCGAAGTTGGTTAAATCAAAAGGTTTTGAGGTGGTTTGGAAAGATTGGGATCATAGTCTGACGGGTAAATAA

Upstream 100 bases:

>100_bases
GCCGGGCGACAGGCATATCTTGCAGGTTTAGCGGGACAGGTGAATCATGCTGTTGCGTCAAGTCCACTGACTGCCTTTTT
ACAGGCGGGGTAAGCAAATT

Downstream 100 bases:

>100_bases
GTCAGTTAATATAATCATAAAGAAGGTGATATGTCAGAGCAAGAAATTTACGCAACGCTGGTGGAAATTAAAGAGGAGCC
AACAGCCCTTTATAAAATAC

Product: thiazole biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MSFSEQIKNYQWDDIRLSIYGKSENDVKRALSKERLDLEDFKALISPAAEPFLEQMAQKSQQLTQQRFGKTQQFFIPLYL
SNMCSNICTYCGFSMHNAIRRKTLDMKEIEDECLAIKKMGFAHILLVTGESERKVGVEYFKQALPIIKKHFSHISIEVQP
LDQHEYEALIEYGVDAVLVYQETYNPVTYAEHHLKGKKSDFKYRLDTHDRLGKAGMLKMGLGCLIGLEEWRTDCFYVAAH
LNYLEKIYWQSRYAISFPRLRPCAGGMEIKSVMDDKELVQLICAYRLFSPEVELSLSTRESEHFRDHVLPLGITSLSAGS
STQPGGYAATSTKALEQFEISDDRSPAKMAKLVKSKGFEVVWKDWDHSLTGK

Sequences:

>Translated_372_residues
MSFSEQIKNYQWDDIRLSIYGKSENDVKRALSKERLDLEDFKALISPAAEPFLEQMAQKSQQLTQQRFGKTQQFFIPLYL
SNMCSNICTYCGFSMHNAIRRKTLDMKEIEDECLAIKKMGFAHILLVTGESERKVGVEYFKQALPIIKKHFSHISIEVQP
LDQHEYEALIEYGVDAVLVYQETYNPVTYAEHHLKGKKSDFKYRLDTHDRLGKAGMLKMGLGCLIGLEEWRTDCFYVAAH
LNYLEKIYWQSRYAISFPRLRPCAGGMEIKSVMDDKELVQLICAYRLFSPEVELSLSTRESEHFRDHVLPLGITSLSAGS
STQPGGYAATSTKALEQFEISDDRSPAKMAKLVKSKGFEVVWKDWDHSLTGK
>Mature_371_residues
SFSEQIKNYQWDDIRLSIYGKSENDVKRALSKERLDLEDFKALISPAAEPFLEQMAQKSQQLTQQRFGKTQQFFIPLYLS
NMCSNICTYCGFSMHNAIRRKTLDMKEIEDECLAIKKMGFAHILLVTGESERKVGVEYFKQALPIIKKHFSHISIEVQPL
DQHEYEALIEYGVDAVLVYQETYNPVTYAEHHLKGKKSDFKYRLDTHDRLGKAGMLKMGLGCLIGLEEWRTDCFYVAAHL
NYLEKIYWQSRYAISFPRLRPCAGGMEIKSVMDDKELVQLICAYRLFSPEVELSLSTRESEHFRDHVLPLGITSLSAGSS
TQPGGYAATSTKALEQFEISDDRSPAKMAKLVKSKGFEVVWKDWDHSLTGK

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=369, Percent_Identity=57.1815718157182, Blast_Score=454, Evalue=1e-129,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 42721; Mature: 42590

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFSEQIKNYQWDDIRLSIYGKSENDVKRALSKERLDLEDFKALISPAAEPFLEQMAQKS
CCHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
QQLTQQRFGKTQQFFIPLYLSNMCSNICTYCGFSMHNAIRRKTLDMKEIEDECLAIKKMG
HHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHCC
FAHILLVTGESERKVGVEYFKQALPIIKKHFSHISIEVQPLDQHEYEALIEYGVDAVLVY
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCEEEEEEEECCCHHHHHHHHHCCCEEEEE
QETYNPVTYAEHHLKGKKSDFKYRLDTHDRLGKAGMLKMGLGCLIGLEEWRTDCFYVAAH
ECCCCCHHHHHHHCCCCCCCCEEEECCHHHCCCCCHHHHHHHHHEEHHHHCCHHHHHHHH
LNYLEKIYWQSRYAISFPRLRPCAGGMEIKSVMDDKELVQLICAYRLFSPEVELSLSTRE
HHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHCCCCEEEEEECCC
SEHFRDHVLPLGITSLSAGSSTQPGGYAATSTKALEQFEISDDRSPAKMAKLVKSKGFEV
HHHHHHHCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEE
VWKDWDHSLTGK
EEECCCCCCCCC
>Mature Secondary Structure 
SFSEQIKNYQWDDIRLSIYGKSENDVKRALSKERLDLEDFKALISPAAEPFLEQMAQKS
CHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
QQLTQQRFGKTQQFFIPLYLSNMCSNICTYCGFSMHNAIRRKTLDMKEIEDECLAIKKMG
HHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHCC
FAHILLVTGESERKVGVEYFKQALPIIKKHFSHISIEVQPLDQHEYEALIEYGVDAVLVY
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCEEEEEEEECCCHHHHHHHHHCCCEEEEE
QETYNPVTYAEHHLKGKKSDFKYRLDTHDRLGKAGMLKMGLGCLIGLEEWRTDCFYVAAH
ECCCCCHHHHHHHCCCCCCCCEEEECCHHHCCCCCHHHHHHHHHEEHHHHCCHHHHHHHH
LNYLEKIYWQSRYAISFPRLRPCAGGMEIKSVMDDKELVQLICAYRLFSPEVELSLSTRE
HHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHCCCCEEEEEECCC
SEHFRDHVLPLGITSLSAGSSTQPGGYAATSTKALEQFEISDDRSPAKMAKLVKSKGFEV
HHHHHHHCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEE
VWKDWDHSLTGK
EEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]