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The map label for this gene is thiF [H]

Identifier: 119944143

GI number: 119944143

Start: 466213

End: 466965

Strand: Direct

Name: thiF [H]

Synonym: Ping_0362

Alternate gene names: 119944143

Gene position: 466213-466965 (Clockwise)

Preceding gene: 119944142

Following gene: 119944144

Centisome position: 10.22

GC content: 44.09

Gene sequence:

>753_bases
ATGTTAACGGATCCTGAAGTATTACGTTATAGCCGTCATCTATTATTGGAAGATGTTGGTGAGTCCGGGCAAAGCGCTTT
GAAATTTTCCAAGGTACTGATTATTGGTATGGGCGGACTCGGCTCACCCGCCGCACTGTATTTAGCCGCTGCGGGGGTAG
GCACACTGGTAATATCTGATTTTGACCATTTGGAAGTGAGCAATCTGCAACGCCAAATCGCTTATCAGAACAGTGATCTA
GGCGCGCCTAAAGTGACCTTAATGAAACAGCGTTTAGAGGCACTTAATCCTGAAGTACGAGTACGCGTCATTAATACTCA
AATGAGTCAATCACAACTTTCGATGGAGCTTATTCTGGCGGATTTAGTCTTAGATTGTACCGATAATATGGCCAGTCGGC
AGATGATTAATAACGCCTGTGTACAGGCGAAAACCCCCTTAATTGTGGGTGCTGCTATTCGCTTCGAAGGGCAGTTAATG
TTTTTTGACCACAGTAAAGCCGATTCTGCTTGTTACCACTGCCTATTCCCCAATAGTGAAGAGCCGGCTTTGAATTGTAG
CAATGCAGGGGTGCTTGGTCCTGTGGTTGGTACCATTGGCACCTTGCAGGCACTTGAGGCATTGAAGTATCTACTTGGTT
TACCTTCAGGCATTAATAATAAATTAAAACTTTTTGATGCTAAAACCTTAGAGTGGCAAACCTTTGTGATTAATAAAGAT
CCCAAATGCCCCGTATGCGGACAAATTCAATGA

Upstream 100 bases:

>100_bases
CCATTGAATCATTTAATCAAGTGATCCAGTAAAAAATAGCCAAGAGTGGTGAGTTTAAGGCTCTCTACTTGAAGTATTCA
TAATAATATTAAGGTAAATC

Downstream 100 bases:

>100_bases
ATATCTTTGTAAACGAACAGGTAATAACAATCCCCGCCAACTGTAATATTGAACAATTATTATTGCTGTTAGAAAGTCCT
TTAAAAGGCAGCGCAGTTGC

Product: adenylyl transferase

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MLTDPEVLRYSRHLLLEDVGESGQSALKFSKVLIIGMGGLGSPAALYLAAAGVGTLVISDFDHLEVSNLQRQIAYQNSDL
GAPKVTLMKQRLEALNPEVRVRVINTQMSQSQLSMELILADLVLDCTDNMASRQMINNACVQAKTPLIVGAAIRFEGQLM
FFDHSKADSACYHCLFPNSEEPALNCSNAGVLGPVVGTIGTLQALEALKYLLGLPSGINNKLKLFDAKTLEWQTFVINKD
PKCPVCGQIQ

Sequences:

>Translated_250_residues
MLTDPEVLRYSRHLLLEDVGESGQSALKFSKVLIIGMGGLGSPAALYLAAAGVGTLVISDFDHLEVSNLQRQIAYQNSDL
GAPKVTLMKQRLEALNPEVRVRVINTQMSQSQLSMELILADLVLDCTDNMASRQMINNACVQAKTPLIVGAAIRFEGQLM
FFDHSKADSACYHCLFPNSEEPALNCSNAGVLGPVVGTIGTLQALEALKYLLGLPSGINNKLKLFDAKTLEWQTFVINKD
PKCPVCGQIQ
>Mature_250_residues
MLTDPEVLRYSRHLLLEDVGESGQSALKFSKVLIIGMGGLGSPAALYLAAAGVGTLVISDFDHLEVSNLQRQIAYQNSDL
GAPKVTLMKQRLEALNPEVRVRVINTQMSQSQLSMELILADLVLDCTDNMASRQMINNACVQAKTPLIVGAAIRFEGQLM
FFDHSKADSACYHCLFPNSEEPALNCSNAGVLGPVVGTIGTLQALEALKYLLGLPSGINNKLKLFDAKTLEWQTFVINKD
PKCPVCGQIQ

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=229, Percent_Identity=43.2314410480349, Blast_Score=150, Evalue=7e-37,
Organism=Homo sapiens, GI4885649, Length=150, Percent_Identity=27.3333333333333, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI87082356, Length=248, Percent_Identity=49.5967741935484, Blast_Score=229, Evalue=1e-61,
Organism=Escherichia coli, GI1787048, Length=247, Percent_Identity=43.7246963562753, Blast_Score=218, Evalue=2e-58,
Organism=Escherichia coli, GI1789177, Length=130, Percent_Identity=29.2307692307692, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17540406, Length=242, Percent_Identity=43.801652892562, Blast_Score=212, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI193203301, Length=147, Percent_Identity=29.2517006802721, Blast_Score=78, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6321903, Length=252, Percent_Identity=33.7301587301587, Blast_Score=140, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6320598, Length=157, Percent_Identity=26.1146496815287, Blast_Score=65, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24582879, Length=249, Percent_Identity=41.3654618473896, Blast_Score=190, Evalue=8e-49,
Organism=Drosophila melanogaster, GI24660640, Length=152, Percent_Identity=28.2894736842105, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012731
- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 27121; Mature: 27121

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTDPEVLRYSRHLLLEDVGESGQSALKFSKVLIIGMGGLGSPAALYLAAAGVGTLVISD
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHEEEEEEECCCCCCHHHHHHHHCCCCEEEECC
FDHLEVSNLQRQIAYQNSDLGAPKVTLMKQRLEALNPEVRVRVINTQMSQSQLSMELILA
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHH
DLVLDCTDNMASRQMINNACVQAKTPLIVGAAIRFEGQLMFFDHSKADSACYHCLFPNSE
HHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEEECCCCCCCEEEEECCCCC
EPALNCSNAGVLGPVVGTIGTLQALEALKYLLGLPSGINNKLKLFDAKTLEWQTFVINKD
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCEEEEEEEEECCC
PKCPVCGQIQ
CCCCCCCCCC
>Mature Secondary Structure
MLTDPEVLRYSRHLLLEDVGESGQSALKFSKVLIIGMGGLGSPAALYLAAAGVGTLVISD
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHEEEEEEECCCCCCHHHHHHHHCCCCEEEECC
FDHLEVSNLQRQIAYQNSDLGAPKVTLMKQRLEALNPEVRVRVINTQMSQSQLSMELILA
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHH
DLVLDCTDNMASRQMINNACVQAKTPLIVGAAIRFEGQLMFFDHSKADSACYHCLFPNSE
HHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEEECCCCCCCEEEEECCCCC
EPALNCSNAGVLGPVVGTIGTLQALEALKYLLGLPSGINNKLKLFDAKTLEWQTFVINKD
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCEEEEEEEEECCC
PKCPVCGQIQ
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 9632726; 10082377 [H]