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The map label for this gene is thiE [H]

Identifier: 119944142

GI number: 119944142

Start: 464642

End: 466144

Strand: Direct

Name: thiE [H]

Synonym: Ping_0361

Alternate gene names: 119944142

Gene position: 464642-466144 (Clockwise)

Preceding gene: 119944141

Following gene: 119944143

Centisome position: 10.19

GC content: 47.37

Gene sequence:

>1503_bases
ATGAATAAACTTGTTTGGAGTATTGGTGCTTCTGATTGCACTGGTGGTGGGGGGATTCAGGCGGATCTCCATACTTTTAA
AGACTTAGGCGCTGATGGCTGCTCGGTGATTAGCGCGGTTACCGCGCAAAACTCGGCGACCGTGAGTTTGGTTGAAAGTG
TATCGGAACAGATGTTCACCGATCAATTAGCCTGTTTAACGGCCGATATGCAGGCAAAGGTGATCAAAATTGGTTTAATT
ACCAGTGTGAAACATGTGCAGATTCTCGCTGAAAAATTGGCTGAATTTAAACAAACCTGGGCGGTGCCACCTTTTGTGAT
TTACGATCCGGTCGCAGTTGCATTGACGGGTGAGAAGATGGCAGAAGAAGGGCTTGTTGAGCTTATTAGAACAGCCTTAC
TACCCTGCATTGATTTGTTAACCGCCAATGCCGGTGAAGTACTTACCTTATCCGGTCATGCTTTAATCAGTGGTGAGTCG
CTAAAACCTGCCGCTTTAAAACTCATCTCGCTGGGCTGTCAAAGTGTCTTGATTAAAGGTGGTGATTTTGAACTGGTGGA
TAAAATAGCGCTTGATTACTGGACAGATGGCAAGCGTGAAATCGCATTAAGCAGCGCACGACTGACTCGTATGGAGAGTC
ACGGCACGGGGGGCATCTTAGCCAGTGCCATTGGCGCAACTATCGCCTTGGGCTACTTTATTGAAGATGCTTTGGTGGTT
GCGAAAGCCTATCTGAATCAAGAGCTAAAAGCGGCCAAAAAGCTTGGCTTCTATGGAATAGCCCATTTAGGCTGGCCAAC
CAATAAAACTGATTTTCCGGAAGTAGTGTTACCCGAGTCAAAAATCGGTTATGAGTTAGATCTGCCGGGGACATTGGAAG
CCGGGCCCGGTTTTGCTTCCTGTGGCACTTTGAAACTGGGGCTATACCCAGTGCTGGACAGTGTTGAATGGTTGGACAAA
GTCCTGAAGTTGGGGGTTAAAACCCTGCAGCTGCGAATTAAAGACAAACAACCGGAACAAGTTGAGCAGCAGATTATTGA
GGCCATTGCCCTTGGACGTAAATATAATGCGCGTTTGTTTATTAATGACTACTGGCAACTGGCCATCAAACATCAGGCCT
ACGGGGTGCATCTTGGTCAGGAAGATATGGACGTTGCGGATTTACCCATGATTGCCGATGCGGGCCTGCGCTTAGGGCTT
AGTACCCATGGTTATTATGAGATATTACGGGCGCAGCAGTTAAAACCCAGTTATATCGCACTGGGGCATATTTTCCCCAC
TCAAACGAAACAGATGCCATCGGATCCGCAGGGGCTAAGCCGTTTAGGAAAATATGCACAGTTGTTATCCGATATTCCAA
CGGTGGCGATTGGCGGCATTAATCTTGAGCGGGCTACCACAGTTTGGAAAACAGGGGTTGGCAGTATTGCAGTGGTTAGC
GCAATAACCCATGCCGACTCTCCCAAAATCGCCATTGAATCATTTAATCAAGTGATCCAGTAA

Upstream 100 bases:

>100_bases
AACGACTCTGGTGCTGAAATTTATCAGCCAGTTTTATAAAAAAGCTGTCGGCTGTCGGCTGTTAGCTGTCAGCAGATAGC
TGCTCTTGTTAAGGATAAAT

Downstream 100 bases:

>100_bases
AAAATAGCCAAGAGTGGTGAGTTTAAGGCTCTCTACTTGAAGTATTCATAATAATATTAAGGTAAATCATGTTAACGGAT
CCTGAAGTATTACGTTATAG

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MNKLVWSIGASDCTGGGGIQADLHTFKDLGADGCSVISAVTAQNSATVSLVESVSEQMFTDQLACLTADMQAKVIKIGLI
TSVKHVQILAEKLAEFKQTWAVPPFVIYDPVAVALTGEKMAEEGLVELIRTALLPCIDLLTANAGEVLTLSGHALISGES
LKPAALKLISLGCQSVLIKGGDFELVDKIALDYWTDGKREIALSSARLTRMESHGTGGILASAIGATIALGYFIEDALVV
AKAYLNQELKAAKKLGFYGIAHLGWPTNKTDFPEVVLPESKIGYELDLPGTLEAGPGFASCGTLKLGLYPVLDSVEWLDK
VLKLGVKTLQLRIKDKQPEQVEQQIIEAIALGRKYNARLFINDYWQLAIKHQAYGVHLGQEDMDVADLPMIADAGLRLGL
STHGYYEILRAQQLKPSYIALGHIFPTQTKQMPSDPQGLSRLGKYAQLLSDIPTVAIGGINLERATTVWKTGVGSIAVVS
AITHADSPKIAIESFNQVIQ

Sequences:

>Translated_500_residues
MNKLVWSIGASDCTGGGGIQADLHTFKDLGADGCSVISAVTAQNSATVSLVESVSEQMFTDQLACLTADMQAKVIKIGLI
TSVKHVQILAEKLAEFKQTWAVPPFVIYDPVAVALTGEKMAEEGLVELIRTALLPCIDLLTANAGEVLTLSGHALISGES
LKPAALKLISLGCQSVLIKGGDFELVDKIALDYWTDGKREIALSSARLTRMESHGTGGILASAIGATIALGYFIEDALVV
AKAYLNQELKAAKKLGFYGIAHLGWPTNKTDFPEVVLPESKIGYELDLPGTLEAGPGFASCGTLKLGLYPVLDSVEWLDK
VLKLGVKTLQLRIKDKQPEQVEQQIIEAIALGRKYNARLFINDYWQLAIKHQAYGVHLGQEDMDVADLPMIADAGLRLGL
STHGYYEILRAQQLKPSYIALGHIFPTQTKQMPSDPQGLSRLGKYAQLLSDIPTVAIGGINLERATTVWKTGVGSIAVVS
AITHADSPKIAIESFNQVIQ
>Mature_500_residues
MNKLVWSIGASDCTGGGGIQADLHTFKDLGADGCSVISAVTAQNSATVSLVESVSEQMFTDQLACLTADMQAKVIKIGLI
TSVKHVQILAEKLAEFKQTWAVPPFVIYDPVAVALTGEKMAEEGLVELIRTALLPCIDLLTANAGEVLTLSGHALISGES
LKPAALKLISLGCQSVLIKGGDFELVDKIALDYWTDGKREIALSSARLTRMESHGTGGILASAIGATIALGYFIEDALVV
AKAYLNQELKAAKKLGFYGIAHLGWPTNKTDFPEVVLPESKIGYELDLPGTLEAGPGFASCGTLKLGLYPVLDSVEWLDK
VLKLGVKTLQLRIKDKQPEQVEQQIIEAIALGRKYNARLFINDYWQLAIKHQAYGVHLGQEDMDVADLPMIADAGLRLGL
STHGYYEILRAQQLKPSYIALGHIFPTQTKQMPSDPQGLSRLGKYAQLLSDIPTVAIGGINLERATTVWKTGVGSIAVVS
AITHADSPKIAIESFNQVIQ

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=196, Percent_Identity=63.7755102040816, Blast_Score=254, Evalue=1e-68,
Organism=Escherichia coli, GI1788420, Length=251, Percent_Identity=32.2709163346614, Blast_Score=117, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6325378, Length=236, Percent_Identity=28.3898305084746, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324517, Length=237, Percent_Identity=30.379746835443, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6324997, Length=237, Percent_Identity=29.535864978903, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6325042, Length=157, Percent_Identity=32.484076433121, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 53691; Mature: 53691

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLVWSIGASDCTGGGGIQADLHTFKDLGADGCSVISAVTAQNSATVSLVESVSEQMFT
CCCEEEECCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
DQLACLTADMQAKVIKIGLITSVKHVQILAEKLAEFKQTWAVPPFVIYDPVAVALTGEKM
HHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEEEEECHHH
AEEGLVELIRTALLPCIDLLTANAGEVLTLSGHALISGESLKPAALKLISLGCQSVLIKG
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEECCCCCHHHHHHHHHCCHHEEEEC
GDFELVDKIALDYWTDGKREIALSSARLTRMESHGTGGILASAIGATIALGYFIEDALVV
CCHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AKAYLNQELKAAKKLGFYGIAHLGWPTNKTDFPEVVLPESKIGYELDLPGTLEAGPGFAS
HHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEECCCCCCCEEECCCCCCCCCCCCCC
CGTLKLGLYPVLDSVEWLDKVLKLGVKTLQLRIKDKQPEQVEQQIIEAIALGRKYNARLF
CCEEEECHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEE
INDYWQLAIKHQAYGVHLGQEDMDVADLPMIADAGLRLGLSTHGYYEILRAQQLKPSYIA
EECCEEEEEEEEEEEEECCCCCCCHHCCCCHHCCCCEECCCCCHHHHHHHHHCCCCCEEE
LGHIFPTQTKQMPSDPQGLSRLGKYAQLLSDIPTVAIGGINLERATTVWKTGVGSIAVVS
EECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCCHHHHHH
AITHADSPKIAIESFNQVIQ
HHHCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MNKLVWSIGASDCTGGGGIQADLHTFKDLGADGCSVISAVTAQNSATVSLVESVSEQMFT
CCCEEEECCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
DQLACLTADMQAKVIKIGLITSVKHVQILAEKLAEFKQTWAVPPFVIYDPVAVALTGEKM
HHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEEEEECHHH
AEEGLVELIRTALLPCIDLLTANAGEVLTLSGHALISGESLKPAALKLISLGCQSVLIKG
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEECCCCCHHHHHHHHHCCHHEEEEC
GDFELVDKIALDYWTDGKREIALSSARLTRMESHGTGGILASAIGATIALGYFIEDALVV
CCHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AKAYLNQELKAAKKLGFYGIAHLGWPTNKTDFPEVVLPESKIGYELDLPGTLEAGPGFAS
HHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEECCCCCCCEEECCCCCCCCCCCCCC
CGTLKLGLYPVLDSVEWLDKVLKLGVKTLQLRIKDKQPEQVEQQIIEAIALGRKYNARLF
CCEEEECHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEE
INDYWQLAIKHQAYGVHLGQEDMDVADLPMIADAGLRLGLSTHGYYEILRAQQLKPSYIA
EECCEEEEEEEEEEEEECCCCCCCHHCCCCHHCCCCEECCCCCHHHHHHHHHCCCCCEEE
LGHIFPTQTKQMPSDPQGLSRLGKYAQLLSDIPTVAIGGINLERATTVWKTGVGSIAVVS
EECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCCHHHHHH
AITHADSPKIAIESFNQVIQ
HHHCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA