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The map label for this gene is bphA [H]

Identifier: 119855031

GI number: 119855031

Start: 151178

End: 152569

Strand: Direct

Name: bphA [H]

Synonym: Mkms_5639

Alternate gene names: 119855031

Gene position: 151178-152569 (Clockwise)

Preceding gene: 119855030

Following gene: 119855032

Centisome position: 50.04

GC content: 59.12

Gene sequence:

>1392_bases
ATGAGTGCTCACGTTCTAGGGGCACAAATCGATAGGAAGGTGCGCCCGGTGGATTCGATGGCGCCTGATGCGACGACAAT
GCGAACCTTAGAGAATGCGCGCGGCTCCATCCTAAAGGGTCGCCTCCCTGCGTCTCTCATCGCTAATGCAGCGCTTTACG
AGCTTGAATTGAAGCGAGTATTTGGTAGGACCTGGCAGTTTCTCTGCCACGAAGACGAGATCCCCAATGCGGGTGACTAT
GTAGTGCGCTACATCGCTGATAACTCAATTATTGTCGCGCGGCAGCAGGATATGACGATTCGGGCGATGTCGAACTCGTG
TCGGCACCGCGGCACGCTGCTTTGCCGAACCGAGTCTGGGAATGAGTCGGCGTTCCAGTGTCCGTACCACGGTTGGACCT
ATCGAAACAACGGTGATCTCATCGCGATACCTGCGCAGCAGGCAGTGTACGGTGCTGCGTTCGACAAGAGTCGGCTAGGG
TTGCGCGCTCTGCCGATGCTGGACTCGTACGCGGGCCTTGTCTTCGGGTGTGTGTCGGATGAGGCGCCGGGACTGGATGA
GTACCTCGGGGACATGCGCTGGTATCTCGACTTGATGATGAAGAAGAGCCCGGCCGGCCTTGAGGCGTGGGGTGCCCCGC
AGCGTTGGGTGATTGACGCGAACTGGAAGACCGGCGCCGATAACTTTGTTGGGGACGGCTATCACACGGTCATGACGCAC
CGTTCGATGTGTGAGCTGGGGTTGTTACCGCCCGATAATGTGGCCGTTTCGCCGGCCCACGTCAGCCTATCGGGCGGGCA
CGGGGCGGGCGTTCTAGGCGCACCACCCGGCATACCCGCACCGCCGTACATGGGCTATCCCGAGGAAGTCGTCTCCGGTC
TCAGCGAGGGTTACGGCGATGACGTCCATGGCGAGTTGCTGAAACGGACGATGTTCATTCATGGCAATGTGTTCCCGAAC
TTGTCCTTCTTGAACGCCTTCATCGCCAAGGACGGGCAGTCTATGCCGATGCCCATTCTGACCTTGCGGCAATGGCGTCC
CTTGGACGCAGCGCGTATGGAGGTGTGGTCGTGGTTCTTCGTGGAGCGCAACGCGCCCGAAGAGTTCAAGCAGCAGTCGT
TTGAGACTTATGTTCGGACGTTCGGGGTCGGGGGTGTCTTCGAGCAGGATGACGCCGAGATATTCCAGGCTATTACCAAG
GGAACACGCGGCGAGTTGGCTGGTGGTGTGGAGCTGAACCTGGAGATGGGACTGGACAATCTGGCTCCTGATCCAACGTG
GCTGGGCCCGGGACGACCGTTGGCCAGTGGCTACGCCGAACAGAATCAGCGCGAGTACTGGAAGCAGTACTTCGACTATC
TGGCCACACCGAGAAGGGATGAGAACGTATGA

Upstream 100 bases:

>100_bases
GCGAGGGGATGCCGGCTGATACCGCCGTTACCGCGGTGGCCTAAATAATAGCTTGCAAACAGTTGTCATCCTATGTAAGC
TTCCGTGTGGTGTAGCCCAC

Downstream 100 bases:

>100_bases
CGACAACGGTGCCGCAGGATCCACCTCAGGCTGCGAGGGTTTCGCGCGACGTGCGCGAGGAAATCGAAGACTTCCTTTTC
GACGAGGCCGATCTGTTGGA

Product: ring hydroxylating dioxygenase, alpha subunit

Products: NA

Alternate protein names: Biphenyl 2,3-dioxygenase [H]

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MSAHVLGAQIDRKVRPVDSMAPDATTMRTLENARGSILKGRLPASLIANAALYELELKRVFGRTWQFLCHEDEIPNAGDY
VVRYIADNSIIVARQQDMTIRAMSNSCRHRGTLLCRTESGNESAFQCPYHGWTYRNNGDLIAIPAQQAVYGAAFDKSRLG
LRALPMLDSYAGLVFGCVSDEAPGLDEYLGDMRWYLDLMMKKSPAGLEAWGAPQRWVIDANWKTGADNFVGDGYHTVMTH
RSMCELGLLPPDNVAVSPAHVSLSGGHGAGVLGAPPGIPAPPYMGYPEEVVSGLSEGYGDDVHGELLKRTMFIHGNVFPN
LSFLNAFIAKDGQSMPMPILTLRQWRPLDAARMEVWSWFFVERNAPEEFKQQSFETYVRTFGVGGVFEQDDAEIFQAITK
GTRGELAGGVELNLEMGLDNLAPDPTWLGPGRPLASGYAEQNQREYWKQYFDYLATPRRDENV

Sequences:

>Translated_463_residues
MSAHVLGAQIDRKVRPVDSMAPDATTMRTLENARGSILKGRLPASLIANAALYELELKRVFGRTWQFLCHEDEIPNAGDY
VVRYIADNSIIVARQQDMTIRAMSNSCRHRGTLLCRTESGNESAFQCPYHGWTYRNNGDLIAIPAQQAVYGAAFDKSRLG
LRALPMLDSYAGLVFGCVSDEAPGLDEYLGDMRWYLDLMMKKSPAGLEAWGAPQRWVIDANWKTGADNFVGDGYHTVMTH
RSMCELGLLPPDNVAVSPAHVSLSGGHGAGVLGAPPGIPAPPYMGYPEEVVSGLSEGYGDDVHGELLKRTMFIHGNVFPN
LSFLNAFIAKDGQSMPMPILTLRQWRPLDAARMEVWSWFFVERNAPEEFKQQSFETYVRTFGVGGVFEQDDAEIFQAITK
GTRGELAGGVELNLEMGLDNLAPDPTWLGPGRPLASGYAEQNQREYWKQYFDYLATPRRDENV
>Mature_462_residues
SAHVLGAQIDRKVRPVDSMAPDATTMRTLENARGSILKGRLPASLIANAALYELELKRVFGRTWQFLCHEDEIPNAGDYV
VRYIADNSIIVARQQDMTIRAMSNSCRHRGTLLCRTESGNESAFQCPYHGWTYRNNGDLIAIPAQQAVYGAAFDKSRLGL
RALPMLDSYAGLVFGCVSDEAPGLDEYLGDMRWYLDLMMKKSPAGLEAWGAPQRWVIDANWKTGADNFVGDGYHTVMTHR
SMCELGLLPPDNVAVSPAHVSLSGGHGAGVLGAPPGIPAPPYMGYPEEVVSGLSEGYGDDVHGELLKRTMFIHGNVFPNL
SFLNAFIAKDGQSMPMPILTLRQWRPLDAARMEVWSWFFVERNAPEEFKQQSFETYVRTFGVGGVFEQDDAEIFQAITKG
TRGELAGGVELNLEMGLDNLAPDPTWLGPGRPLASGYAEQNQREYWKQYFDYLATPRRDENV

Specific function: Part Of The Multicomponent 3-Phenylpropionate Dioxygenase. Converts 3-Phenylpropionic Acid (Pp) Into Cis-3-(3- Carboxyethyl)-3,5-Cyclohexadiene-1,2-Diol (Pp-Dihydrodiol). [C]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=397, Percent_Identity=35.7682619647355, Blast_Score=245, Evalue=5e-66,
Organism=Escherichia coli, GI1788103, Length=207, Percent_Identity=30.9178743961353, Blast_Score=93, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: =1.14.12.18 [H]

Molecular weight: Translated: 51369; Mature: 51238

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00570 RING_HYDROXYL_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAHVLGAQIDRKVRPVDSMAPDATTMRTLENARGSILKGRLPASLIANAALYELELKRV
CCCCCCCHHHCCCCCCCHHCCCCHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHH
FGRTWQFLCHEDEIPNAGDYVVRYIADNSIIVARQQDMTIRAMSNSCRHRGTLLCRTESG
HHHHHHHHCCCCCCCCCCCEEEEEEECCCEEEEECCCCEEEHHHHHHHCCCEEEEEECCC
NESAFQCPYHGWTYRNNGDLIAIPAQQAVYGAAFDKSRLGLRALPMLDSYAGLVFGCVSD
CCCEEECCCCCCEEECCCCEEEECCHHHHHHHHCCCCCCCCEECCHHHHHHHHEEEECCC
EAPGLDEYLGDMRWYLDLMMKKSPAGLEAWGAPQRWVIDANWKTGADNFVGDGYHTVMTH
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHH
RSMCELGLLPPDNVAVSPAHVSLSGGHGAGVLGAPPGIPAPPYMGYPEEVVSGLSEGYGD
HHHHHHCCCCCCCEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
DVHGELLKRTMFIHGNVFPNLSFLNAFIAKDGQSMPMPILTLRQWRPLDAARMEVWSWFF
CHHHHHHHHHHEEECCCCCCHHHHHHHHHCCCCCCCCCHHHHCCCCCCCHHHHHHHEEEE
VERNAPEEFKQQSFETYVRTFGVGGVFEQDDAEIFQAITKGTRGELAGGVELNLEMGLDN
EECCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCC
LAPDPTWLGPGRPLASGYAEQNQREYWKQYFDYLATPRRDENV
CCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SAHVLGAQIDRKVRPVDSMAPDATTMRTLENARGSILKGRLPASLIANAALYELELKRV
CCCCCCHHHCCCCCCCHHCCCCHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHH
FGRTWQFLCHEDEIPNAGDYVVRYIADNSIIVARQQDMTIRAMSNSCRHRGTLLCRTESG
HHHHHHHHCCCCCCCCCCCEEEEEEECCCEEEEECCCCEEEHHHHHHHCCCEEEEEECCC
NESAFQCPYHGWTYRNNGDLIAIPAQQAVYGAAFDKSRLGLRALPMLDSYAGLVFGCVSD
CCCEEECCCCCCEEECCCCEEEECCHHHHHHHHCCCCCCCCEECCHHHHHHHHEEEECCC
EAPGLDEYLGDMRWYLDLMMKKSPAGLEAWGAPQRWVIDANWKTGADNFVGDGYHTVMTH
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHH
RSMCELGLLPPDNVAVSPAHVSLSGGHGAGVLGAPPGIPAPPYMGYPEEVVSGLSEGYGD
HHHHHHCCCCCCCEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
DVHGELLKRTMFIHGNVFPNLSFLNAFIAKDGQSMPMPILTLRQWRPLDAARMEVWSWFF
CHHHHHHHHHHEEECCCCCCHHHHHHHHHCCCCCCCCCHHHHCCCCCCCHHHHHHHEEEE
VERNAPEEFKQQSFETYVRTFGVGGVFEQDDAEIFQAITKGTRGELAGGVELNLEMGLDN
EECCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEEEECCCC
LAPDPTWLGPGRPLASGYAEQNQREYWKQYFDYLATPRRDENV
CCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1569021; 7592331 [H]